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Citations for Thermo Fisher :
151 - 200 of 1033 citations for Ebola Virus Nucleoprotein NP Zaire since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and Moloney murine leukemia virus reverse-transcriptase (MMLV-RT, Invitrogen). Subsequent PCR amplification performed in a thermocycler using specific primers ...
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bioRxiv - Genetics 2020Quote: Fibroblasts were infected by Sendai virus(Thermo Fisher Scientific, A16517) Transfected fibroblasts (approximately 1*105 cells per nucleofection ...
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bioRxiv - Microbiology 2021Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix (sequences shown in Table 1) ...
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bioRxiv - Immunology 2020Quote: ... the virus inoculum was replaced with fresh medium (MEM, Gibco) containing 1.25% Avicel® (FMC Biopolymer ...
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bioRxiv - Immunology 2021Quote: ... Virus was diluted in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) and maintained on ice prior to infection ...
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and virus amplification in Spodopetera frugiperda 9 (Sf9) cells (ThermoFisher) was performed using standard procedures and as described in Zeqiraj et al ...
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bioRxiv - Immunology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific), primers ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus (M-MLV) Reverse Transcriptase (Invitrogen). One microgram of total RNA was retrotranscribed and the cDNA was diluted 1:10 to the quantitative PCR (qPCR) ...
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bioRxiv - Immunology 2024Quote: ... Virus inoculum was prepared in DMEM (Thermo Fisher Scientific, 119905040) supplemented with 2% FBS and allowed for 1 hour at 37°C for virus adsorption onto cells ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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bioRxiv - Immunology 2021Quote: MHC-I tetramer targeting the immunodominant epitope of the influenza nucleoprotein (DbNP366-374 – ASNENMETM, DbPA224-233 - SSLENFRAYV) was produced in-house and conjugated to streptavidin-APC/PE (Life Technologies Australia Pty Ltd) at a 1:250 dilution at room temperature for 1h ...
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bioRxiv - Biophysics 2020Quote: ... NPs preparation starts with their incubation in 10 μM Neutravidin (31000, Thermo Fisher) for 20 min at 25 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... or NP-40 buffer supplemented with 1x Halt protease inhibitor cocktail (Thermo Scientific) and 1x Halt phosphatase inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1% NP-40 with 1x Halt protease inhibitor cocktail (Thermo Fisher Scientific, 78425), 1 mM PMSF ...
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bioRxiv - Microbiology 2020Quote: ... Triton X-100 and NP-40 Surfact-Amps Detergent Solutions (all Thermo Scientific), and UltraPure SDS 10% solution (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5% NP-40) supplemented with HALT protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) for 30 min at 4°C before clarification.
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bioRxiv - Cancer Biology 2019Quote: ... 1% NP-40.) supplemented with 1μl of 100x Protease inhibitor cocktail (ThermoFisher Scientific). Lysate was incubated for five minutes at room temperature before centrifugation at 16,000 x g at 4oC for 10 minutes (Eppendorf Centrifuge 5415R) ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.45% NP-40) containing 0,1 mg/mL Proteinase K (EO0491;Thermo Fisher Scientific) and incubating for 1h at 56°C followed by a 10 min heat inactivation of Proteinase K by 96°C ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were washed with digestion buffer (ThermoFisher Scientific ER0932 plus 0.2% NP-40), pelleted at 6,000 xg for 5 min and incubated with digestion buffer (ThermoFisher Scientific ER0932 plus 0.2% NP-40 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed in NP-40 lysis buffer (Thermo Scientific, no. J60766.AP) supplemented with protease inhibitor (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole-cell extracts were prepared using NP-40 lysis buffer (Thermo Fisher Scientific) supplemented with complete protease inhibitor cocktail (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 mM MgCl2 and 0.5% NP-40) substituted with RNase Inhibitor (Thermo Fisher) and incubated on ice for 5mins ...
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bioRxiv - Microbiology 2022Quote: ... and then stained with monoclonal anti-SARS-NP antibody conjugated to AlexaFluor488 (Invitrogen) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% NP-40) supplemented with HALT protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) for 30 min at 4°C before clarification ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were prepared in serum-free VP-SF media (Invitrogen). Viruses were harvested before CPE development ...
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bioRxiv - Cell Biology 2021Quote: ... The virus packaging was performed in HEK293FT cells (Thermo Fisher Scientific) based on a calcium precipitation method using pUMVC and pCMV-VSV-G vectors (37 ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). According to the manufacturer’s instruction (QIAamp Viral RNA mini kit (Qiagen)) ...
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bioRxiv - Microbiology 2022Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 genomic RNA was amplified and detected using forward (5’- CGTGTAGTCTTTAATGGTGTTTCC-3’ ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... TaqMan™ Fast Virus 1-Step Master Mix (4444432, Life Technologies) was used for one-step RT-qPCR and TaqMan Fast Advanced Master Mix (4444556 ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were treated with 40 U/mL DNase I (Invitrogen) for 1 hr at 37°C before they were used to infect cells for 2 hr at an MOI of 0.5-2 ...
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bioRxiv - Physiology 2022Quote: ... and 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final reaction volume of 20 μL (Evans-Molina et al. ...
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bioRxiv - Microbiology 2020Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 N gene RNA was amplified using forward (5’-GACCCCAAAATCAGCGAAAT ...
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bioRxiv - Genetics 2020Quote: ... Cells were then transduced using CytoTune-iPS 2.0 Sendai virus (Invitrogen) as outlined in the supplier’s protocol at an MOI of 5:5:3 (KOS [Klf4 ...
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bioRxiv - Microbiology 2021Quote: Virus replication assays were performed in RPMI 1640 (Gibco 11875-093) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... 5 μl of TaqMan Fast Virus 1-Step Master Mix (ThermoFisher), and 9 μl of molecular-grade water per reaction ...
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bioRxiv - Immunology 2023Quote: ... TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat No. 5555532) and a commercially prepared RNAse P primer/probe combination (IDT ...
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bioRxiv - Immunology 2023Quote: ... Taqman Fast Virus 1-step kit (Thermo Fisher; catalog number 4444434) with oligos and probe specific to the N gene of SARS-CoV2 was used for SARS-CoV-2 RNA detection according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
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bioRxiv - Molecular Biology 2023Quote: Virus particles were fixed in 1 % glutaraldehyde (Cat# 233281000, Thermo Scientific). A 4 µL aliquot of sample was adsorbed onto holey carbon-coated grid (Lacey ...
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bioRxiv - Cell Biology 2020Quote: ... 0.3% NP-40 and 1mM DTT] and Halt protease inhibitor cocktail (Thermo Fisher Scientific) with incubation on ice for 5 min ...
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bioRxiv - Genomics 2019Quote: ... 5 mM EDTA and 0.5% NP-40 supplemented with 1U/μl SUPERase-In (Invitrogen) and 1x cOmplete protease inhibitor (Roche)] followed by preclearing with 50 μl Dynabeads G (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... As-prepared AuCOOH NPs were collected by centrifugation (Sorvall RC-6 Plus, Thermo Scientific) at ~11,000g for 1 h and thrice washed with DI water ...
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bioRxiv - Cell Biology 2019Quote: ... and 1% NP-40 with 2X Halt Protease Inhibitor Cocktail (78430, Thermo Fisher Scientific) and then sonicated on ice (Sonifier 250 ...
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bioRxiv - Genomics 2021Quote: ... The proteins were extracted in NP-40 cell lysis buffer (Thermo Fisher Scientific, #FNN0021) with HaltTM phosphatase inhibitor Cocktail (Thermo Fisher Scientific ...