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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... in blocking solution with the addition of the following secondary antibodies (dependent on primary antibody used, all secondary antibodies were used at 1:1000): donkey anti-goat A647 (Invitrogen A-21447), donkey anti-rabbit A488 (Invitrogen A-21206) ...
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bioRxiv - Plant Biology 2019Quote: ... First strand cDNA synthesis was performed from 1 µg – 2.5 µg of total RNA using Superscript II RT (Invitrogen) with oligo(dT ...
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bioRxiv - Microbiology 2019Quote: ... 1 kb regions up- and downstream of sosA were PCR amplified (Phusion Hot Start II DNA Polymerase, Thermo Scientific) using primer pairs Up-sosA_fw-Kpnl/Up-sosA_rev and Dw-sosA_fw/Dw-sosA_rev-SacI ...
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bioRxiv - Systems Biology 2021Quote: We isolated pre-adipocyte cells from subcutaneous adipose tissue by dissecting tissue into small (1-2 mm) pieces with scissors and performing a 0.1% (w/v) collagenase II (Gibco) digestion in DMEM at 37oC for 1 hour ...
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bioRxiv - Evolutionary Biology 2020Quote: ... stained for 20 min in SYBR™ Green II RNA Gel Stain (1:10,000 in 0.5x TBE, ThermoFisher Scientific) and imaged on the iBright™ (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Each 20-μlL PCR mixture contained 2 μL of 10X Accuprime PCR buffer II (Life Technologies, CA, 1 USA), 0.15 μL of Accuprime high-fidelity polymerase (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... BS-C-1 cells were seeded into Nunc™ 8-well Lab-Tek II chambered coverglass slides (Thermo Scientific) to obtain 50 to 70% confluence on the day of tracking ...
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bioRxiv - Genomics 2020Quote: ... pCVM-G pCMV-rev and pHIV7-IMPDH2-U6 at 1:0.5:0.25:1.5 ratio using SuperScript II and PLUS reagent (Thermo Fisher Scientific) in serum depleted DMEM media ...
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bioRxiv - Immunology 2020Quote: ... 1×106 CAR T cells were co-cultured with γ-irradiated K562-CD19 or K562-CD19-PD-L1 at 1:3 effector:target (E:T) ratio for 7 days and counted with the countess II Automated Cell Counter (ThermoFisher, USA).
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bioRxiv - Biochemistry 2020Quote: ... Total RNA samples (1 μg) were reverse-transcribed using RNase H-MMLV reverse transcriptase (Superscript II, Invitrogen, Carlsbad, California) and Oligo (dT)™ primers (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 mM DTT and 1.25 mM dNTPs) and 1 µL of Superscript II RT (Thermo Fisher, 200 U/µL) for 42 °C for 3 hours ...
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bioRxiv - Molecular Biology 2020Quote: BMDMs were seeded at 1×105 cells/well in 8 wells Nunc Lab-Tek II Chamber Slide (Thermo Scientific). The next day ...
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bioRxiv - Developmental Biology 2021Quote: ... Reverse transcription was performed from 1 μg of total RNA using SuperScript™ II Reverse Transcriptase (Thermo Fisher Scientific). Quantitative reverse transcription polymerase chain reaction (qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... a microdispenser machine Nanodrop II (BioNex) was used to dispend in each well the ERCC spike-in control RNAs (1:50,000, Invitrogen), reagents used for the reverse transcription (RT ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 μg of total RNA was used to synthesize complementary DNA using random primers (SuperScript II RT; Invitrogen). For quantitative PCR ...
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bioRxiv - Microbiology 2020Quote: ... The Madin-Darby Canine Kidney II (MDCK-II) cell line was maintained in Eagle’s Minimum Essential Medium (EMEM, Gibco), supplemented with 5% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... Gateway recombination reactions were performed with BP Clonase II and LR clonase II enzyme mixes (Invitrogen, Carlsbad, CA, USA) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... followed by injection of 20 ml of pre-warmed digestion medium (1 mg/ml collagenase P, 1 mg/ml dispase II, 50 µg/ml DNase I in Gibco Hank’s Balanced Salt Solution (HBSS) with 10 mM HEPES and 1.5 mM calcium chloride ...
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bioRxiv - Cell Biology 2019Quote: ... clone K124C (1:10 dilution) served as primary antibody and goat anti-mouse Alexa Fluor™594 antibody (1:500 dilution; Invitrogen, Thermo Fisher Scientific) as the secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the secondary antibodies (biotinylated goat anti-rat antibody, Jackson 112-066-072, 1/500; goat anti-rabbit Alexa fluor 635, Life technologies A31577, 1/500) and DAPI (4/1000 ...
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bioRxiv - Bioengineering 2020Quote: ... (primary Rabbit anti-mouse alpha-SMA antibody; Cat. No. ab5694; Abcam; 1:50 dilution; secondary Goat anti-rabbit antibody; Cat. No. A11008; Life Technologies; 1:150 dilution). Fluorescent images were acquired using a Leica SP8 confocal microscope ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed three times in 1X PBS and then incubated with the appropriate PLA-antibodies (PLAminus, PLAplus, Duolink, 1:10) diluted in blocking buffer and a secondary antibody to visualize MAP2 (A488, ThermoFisher, A-11073, 1:1000) for one hour in a pre-warmed humidified chamber at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were permeabilised with phosphate buffer for 2 hours and then incubated with self-made anti-HA antibody (1:500) followed by Alexa488-conjugated secondary antibody (1:2,000) (Life Technologies, Thermo Fisher Scientific A11008). Specimens were mounted with Roti®-Mount FluorCare DAPI (Carl Roth ...
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bioRxiv - Bioengineering 2022Quote: ... followed by incubation with secondary antibodies for 1 hour by using an Alexa Fluor-488 anti-mouse IgG antibody (Thermo Fisher Scientific, 1:250), Alexa Fluor-488 anti-rat IgG antibody (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and subsequently for 16 hours at 4°C in blocking solution containing primary antibodies (anti-A2AR mouse antibody, sc-32261 Santa Cruz Biotechnology, at 1:100 and anti-CD31 rat monoclonal antibody, ER-MP12 Thermo Scientific, 1:20). For detection of A2AR and CD31 ...
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bioRxiv - Cell Biology 2022Quote: ... The following commercially available monoclonal antibodies were used in this study: monoclonal mouse anti-myc antibody (Invitrogen, 132500; IB 1:2’000, IF 1:50), monoclonal mouse anti-HA antibody (Enzo Life Sciences AG ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary antibodies used were the following: Alexa 488/Alexa 594 (both 1:1000) /Alexa 647 (1:500) -conjugated secondary antibodies (Invitrogen; Villebon sur Yvette, France) directed against rabbit /rat polyclonal or mouse monoclonal primary antibodies.
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bioRxiv - Neuroscience 2022Quote: ... A subset of sections from brains with FLEX-SomArchon expression were stained with a guinea pig anti-PV antibody (GP72, Swant, 1:1000) following by Alexa Fluor 568 goat anti-guinea pig secondary antibody (No: A-11075, Thermo Fisher Scientific, 1:500). All antibodies were diluted in 2% normal goat serum and 0.5% Triton-X (No ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained for 1h at RT with a monoclonal Anti-HA tag antibody [16B12] (ab130275, 1/100) and a secondary goat anti-mouse antibody labeled with Alexa 546 (Thermofisher #A-21123, 1/500) then counterstained with Prolong Gold mounting medium containing DAPI (Life Technologies #P36941).
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bioRxiv - Cell Biology 2023Quote: ... or detected with a monoclonal mouse antibody (SantaCruz, sc-245, 1:250) and visualised with a donkey anti-mouse Alexa Fluor 647 secondary antibody (Molecular Probes, A31571, 1:1000). SCC1/ RAD21 was detected with a monoclonal mouse antibody (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NK-CD16 cells with positive SmBiT expression were selected via cell sorting by probing HA tag (anti-HA antibody [Invitrogen #26183, 1:500]; Anti-mouse secondary antibody Alexa Fluor 647 [Invitrogen #A-21235, 1:200]), probing CD16 (anti-CD16 antibody [Abcam #246222 ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were diluted in 1 % non-fat dry milk in TBS-T: mouse anti-TFR1 antibody (1:1,000, Thermo Fisher Scientific, 13-6800, RRID:AB_2533029), rabbit anti-FPN antibody (1:2,000 ...
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bioRxiv - Microbiology 2023Quote: ... and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A, custom antibody from Thermo Fisher 1:1000). Secondary antibody staining was performed using an anti-rabbit antibody (Azure anti-rabbit NIR700 or Azure anti-rabbit NIR800 at 1:20000 dilution) ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody-bound membranes were incubated in fluorescence-conjugated secondary antibodies (AF680, Life Technologies; AF800, LiCor; 1:5000). Fluorescent images of the membranes were obtained using the Odyssey CLx infrared imaging system ...
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bioRxiv - Microbiology 2020Quote: ... slides were incubated with secondary antibodies at 1:200 (Alexa 633 Goat anti-Rabbit antibody (Invitrogen A21071) and Alexa 488 Goat anti-Mouse antibody (Life technologies A11029) ...
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bioRxiv - Neuroscience 2021Quote: ... with diluted secondary antibodies: Alexa-fluor 555/647 Donkey anti Rabbit/Goat IgG antibodies (Invitrogen, 1:400).
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bioRxiv - Molecular Biology 2020Quote: ... Antibody binding was detected with secondary antibodies: goat anti-mouse (1:2500) (Thermo Fisher Scientific, 62-6520), mouse anti-rabbit (1:3000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody binding was detected with secondary antibodies: goat anti-mouse (1:2500) (Thermo Fisher Scientific, 62-6520) and mouse anti-rabbit (1:3000 ...
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bioRxiv - Neuroscience 2020Quote: The following primary antibodies were used—rabbit polyclonal GFP antibody 1:1000 (Molecular Probes, Eugene, Oregon, USA), mouse anti-GFP 1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Dilution of the secondary antibodies are as follows: Anti-rabbit Alexa Fluor 568 antibody (1:200; Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... revealed with an Alexa Fluor 555-coupled secondary antibody against rabbit antibodies (Molecular Probes A27039, 1/500). 2×107 cells per round 25 mm coverslip were harvested and fixed using the sequential PFA methanol protocol described above ...
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bioRxiv - Neuroscience 2020Quote: ... antibodies were probed overnight followed by incubation for 1 hour with secondary antibodies conjugated to HRP (Invitrogen). Densitometry was performed using ImageJ (Schneider ...
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bioRxiv - Biophysics 2020Quote: ... The membranes were incubated with the primary V5 HRP-conjugated antibody (1:5,000, V5-HRP antibody, Invitrogen) in TTBS with 5% blotting-grade blocker overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... source: Geneva antibody facility) and secondary antibody anti-guinea pig Alexa 488 (1:400 dilution, source: ThermoFisher). Images were acquired on a Leica TCS SP8 microscope ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with primary antibodies overnight at 4 °C (1:100 mouse anti CYP3A4 antibody, Life Technologies MA5-17064 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were incubated with appropriate fluorophore-conjugated secondary antibodies (1:1000, Life Technologies, Carlsbad, California, USA) depending on the desired fluorescence colour.
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibodies used were: Alexa-488 and Alexa-568 conjugated anti-rat antibody (Molecular Probes; 1:200), Alexa-488 and Alexa-568 conjugated anti-mouse antibody (Molecular Probes ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The antibody was detected with a fluorescently labeled secondary donkey-anti-mouse antibody (Invitrogen, dilution 1:200) with Alexa Fluor 488 ...
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bioRxiv - Genomics 2019Quote: ... The following primary antibodies were used in this study: chicken GFP polyclonal antibody (1:500, Invitrogen, A10262); rat anti-Deadpan (1:100 ...
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bioRxiv - Developmental Biology 2019Quote: ... Secondary antibodies used in this study were: Alexa546 fluorophore-conjugated secondary antibody (1:500, Invitrogen Molecular Probes), Alexa488 fluorophore-conjugated secondary antibody (1:500 ...