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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Streptavidin ZAP Antibody Kit rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Sections were subsequently washed in multiple changes of PBS and incubated with fluorescent dye–conjugated secondary antibodies (1:1,000; mouse, rat, or rabbit; Alexa Fluor; Life Technologies) in gel block or MOM-containing gel block when appropriate ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of CD4-expressing cells was measured after incubation for 10 minutes with 1 µl of anti-CD4 antibody coupled to Alexa 647 (rat isotype, RM4-5, Invitrogen). For each cell line ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the tissues were incubated for 24 hours in secondary goat anti-rat IgG antibodies: Alexa Fluor 488 conjugated (Molecular Probes, Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Donkey anti-Mouse Alexa Fluor 568 (1:500, no. A10037), and Donkey anti-Rat Alexa Fluor 594 (1:500, no. A21209)–conjugated secondary antibodies were from ThermoFisher Scientific.
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed twice with PB then incubated with species-specific secondary antibodies diluted in PBS-T for two hours (goat anti-rat conjugated to Alexa 488; ThermoFisher; #A-11006 ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibody: goat-derived anti-Rat IgG (H+L) conjugated to Alexa Fluor 488 (AF488) (dilution 1:250; Thermo Fisher). Samples were incubated at room temperature for 1h in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were then stained using a rat anti-tubulin primary antibody solution (diluted 1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4°C overnight incubation ...
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bioRxiv - Molecular Biology 2022Quote: mLECs were isolated from collagenase I-digested lung tissue using rat anti-mouse CD31 monoclonal antibody-coated Dynabeads (11035, Invitrogen) and directly used for RNA analysis.
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... 1:100) followed by anti-rat secondary antibody conjugated with Alexa Fluor 647 (Thermo Fisher, catalog no. A21247, 1:200). Subcapsular sinus macrophages were labeled using anti-sialoadhesin (CD169 ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were first incubated with rat anti-HA (1:100) antibody overnight at 4°C followed by donkey anti-rat Alexa Fluor (AF) 488 (1:500; Invitrogen) for 1.5 h at 20°C before staining for other markers ...
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bioRxiv - Immunology 2022Quote: ... the cells were incubated for 90 min at 37 °C with AlexaFluor-488 conjugated goat anti-mouse and AlexaFluor-568 goat anti-rat antibodies and DAPI (Invitrogen). Fluorescence microscopy was performed using a Nikon Structured illumination microscope (N-SIM ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with the primary antibodies (rabbit anti–GFP, 1:1,000 dilution; rat anti–mCD8, 1:200 dilution, ThermoFisher Scientific) at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... used for nNOS immunolabeling, and 568-conjugated donkey anti-rat secondary antibody (1:500; Cat#?, Lot#) used for GnRH were purchased from Invitrogen.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-mouse or anti-rat or anti-rabbit fluorescein-conjugated secondary antibodies (dilution 1:500, Invitrogen) and cell nuclei were stained with DAPI (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-rat Alexa 488 and goat anti-mouse IgM Alexa 555 (dilution 1:250; all secondary antibodies were obtained from Invitrogen). Specificity of the secondary antibody was verified by including a control staining in which the primary antibody was omitted (data not shown) ...
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bioRxiv - Synthetic Biology 2023Quote: ... the samples were again incubated overnight with secondary antibodies (ThermoFisher, anti-rat polyclonal conjugated with Alexa 594, 1:300; ThermoFisher anti-rabbit polyclonal conjugated with STAR RED 1:300 ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked in 5% BSA in PBS and rat-anti-DILP2 was incubated overnight at 4°C (Geminard et al., 2009) followed by Alexa488 anti-rat-secondary antibodies (Invitrogen). Tissues were washed and mounted in Vectashield with DAPI (Vector Laboratories ...
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bioRxiv - Developmental Biology 2023Quote: ... This was followed by overnight incubation in the goat anti-rat Alexa Fluor 647 secondary antibody (A21247, ThermoFisher, 1:500). The embryos were washed with PBT 4X15 min and kept at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were incubated with horseradish peroxidase-conjugated (HRP-linked) secondary antibodies anti-rat IgG (ThermoFisher Scientific, 31470, 1:5000), anti-rabbit IgG (Promega ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Secondary antibodies were: goat anti-rat Alexa Fluor 647 (1:500, for anti-N-Cadherin, Life Technologies, cat. no. A21247), goat anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were stained for 1 hour at room temperature with anti-rat secondary antibody (Alexa Fluor 488; Thermo Fisher Scientific) followed by 2 minutes of Sudan Black (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with goat anti-rat IgG (H+L) secondary antibody tagged with Alexa Fluor 488 (Invitrogen, A-21470). Tissue sections were washed and blocked with 1:100 mouse Fc block in 5% goat serum ...
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bioRxiv - Immunology 2023Quote: ... Sections were then washed with 1% goat serum and 1% triton for 30 minutes and were incubated with the secondary antibody -anti-rat Alexa fluor647 (1:500, ThermoFisher)-for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... sections were probed with biotinylated monoclonal antibody E492 (3 µg/mL; steamer pH 6.1) alongside a monoclonal antibody either against Clec4F (clone 370901, rat IgG2a, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed with TBS 3 times and incubated with donkey secondary antibodies (1:500, Alexa-Fluor 488/594/647 anti-rabbit, anti-mouse, or anti-rat, Invitrogen, A21202 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times with PBS and subjected to conjugated secondary antibody diluted in IFF buffer (anti-Mouse-AF488 A21202 Invitrogen, anti-Rabbit-568 A11036 Invitrogen, anti-Rat-AF647 A78947 Invitrogen) at 1:2000 for 2h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow cells from MycWT/WT or MycT58A/T58A mice were first depleted using purified lineage antibodies described above either using anti-rat IgG Dynabeads (ThermoFisher) or MACS lineage depletion beads on an AutoMACS system (Miltenyi Biotech) ...
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bioRxiv - Developmental Biology 2024Quote: ... for two nights at 4°C and then with Alexa Fluor 555-conjugated Donkey anti-rat secondary antibody (1:50; Cat. No. A11077, Invitrogen). Light-sheet images were captured by LiTone XL Light-sheet Microscope (Light Innovation Technology Limited ...
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bioRxiv - Cell Biology 2024Quote: ... Microtubules were then stained using the rat anti-tubulin primary antibody (1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4 °C overnight incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... AlexaFluor555 (anti-mouse) and AlexaFluor488 (anti-rat, both secondary antibodies were from Invitrogen by Thermo Fisher Scientific, Waltham, MA, USA) for 45 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated for 3 hours at RT in secondary antibody (anti-rat AlexaFluor-488, #A21208, or anti-mouse AlexaFluor-488, Invitrogen #A11001 ...
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bioRxiv - Neuroscience 2024Quote: ... Appropriate secondary antibodies were used (1:500, goat anti-rat Alexa 488 Invitrogen A11006; 1:500, goat anti-rabbit Alexa 555 Invitrogen A21429 ...
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bioRxiv - Neuroscience 2024Quote: ... Remaining cells were washed with PBS and blocked with 10% rat serum and then incubated with following fluorescent monoclonal antibodies from Invitrogen: CD11b eFluor450 (Clone M1/70) ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysate was centrifuged for 30 min at 15000 g and supernatant was applied to streptavidin-conjugated magnetic beads (Dynabeads® MyOne™ Streptavidin C1, Invitrogen, # 65001). Beads were extensively washed 5x with lysis buffer followed by 5x washes with ice-cold PBS ...
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bioRxiv - Microbiology 2020Quote: ... After sonication samples were spun at 16,000 × g for 20 min at 4°C and the supernatant was added to 400 µl of streptavidin resin (Pierce® High Capacity Streptavidin-agarose Resin, Thermo Scientific Pierce) and incubated overnight at 4°C.
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bioRxiv - Microbiology 2022Quote: ... The biotin-marked ligation products were then enriched by affinity capture with streptavidin magnetic beads (DynaBeads MyOne Streptavidin C1; Invitrogen, Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... and allowed to incubate in streptavidin for at least three days (1:500 Streptavidin, Alexa Fluor™ 488/647 conjugate, ThermoFisher S11223/S21374). The tissue was then washed 3 x 5 minutes in 0.01M PBS ...
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bioRxiv - Immunology 2024Quote: ... Antibody conjugation was performed in house for some of the antibodies using the antibody labelling kit (Invitrogen).
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bioRxiv - Physiology 2022Quote: ... primary antibodies were labeled directly using Zenon antibody labeling kits (Thermo Fisher Scientific) for AF546 or AF647 as described (22 ...
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bioRxiv - Developmental Biology 2022Quote: ... For antibody coupling using the Dynabeads™ Antibody Coupling Kit (Thermo Fisher Scientific) 10 µg antibody per mg beads were used ...
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bioRxiv - Immunology 2024Quote: ... Anti-Aqp3 antibody was labeled using the Zenon antibody labeling kit (Molecular Probes). FACS results were plotted and analyzed using FlowJo software (TreeStar).
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bioRxiv - Bioengineering 2021Quote: ... were sub-cultured on 0.1 mg/ml rat tail collagen type I (rat tail collagen I, ThermoFisher) T-75 coated flasks and passaged at about 80% confluency ...
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bioRxiv - Synthetic Biology 2019Quote: ... The newly generated RNA transcripts were separated from the streptavidin magnetic beads and their quantity measured using the Qubit RNA HS Assay Kit (Thermo Fisher, Q32852).
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bioRxiv - Genomics 2019Quote: ... The DNA fragments biotinylated at 8-oxoG were eluted with 125 µl Tris buffer using the QIAquick PCR purification kit and extracted using Dynabeads MyOne Streptavidin C1 (Thermo Fisher Scientific); strands complementary to those with bound biotinylated 8-oxoG were released by incubation in 150 mM NaOH at 20 °C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... tertiary processing was used to enhance the strength of the punctate labeling in which a biotinylated secondary antibody (1:200) was followed by fluorescently conjugated streptavidin (SA-Alexa488, Invitrogen, 1-2 µg/ml).
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bioRxiv - Neuroscience 2020Quote: ... washed in the permeabilizing solution for 1 day, and incubated in the secondary antibody (goat anti-rabbit Alexa Fluor 488, 1:200) and Streptavidin-Alexa 594 (ThermoFisher Scientific S11227, 1:100) for 6 days at 4°C.
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated with the secondary antibodies goat anti-mouse Alexa Fluor® 568 (1:500) and Alexa Fluor® 488 conjugated to streptavidin (1:500) (Invitrogen, MA, USA) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... the libraries were labeled with streptavidin (SA)-phycoerythrin (PE) (eBioscience, 12-4317-8) and goat anti-mouse-Alexa Fluor 647 antibodies (Thermo Fisher Scientific, A-21235). The population with a weak PE signal and a strong Alexa Fluor 647 signal was sorted in a FACSAria II (BD ...
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bioRxiv - Cell Biology 2022Quote: ... IgG and anti-streptavidin treated PS DAAM-particles were stained with donkey anti-rabbit-Alexa Fluor-647 antibodies (Invitrogen, A31573, 1:1000 in PBS) for 30 min ...