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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Saint Louis Encephalitis Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Protein concentrations were quantified using Pierce™ BCA Protein Assay kit (ThermoFisher, 23225) and normalized before proteins were loaded on 4∼20% Tris-Glycine gels for electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined using Pierce® BCA Protein Assay Kit (Thermo Scientific). Equal amounts of protein (20-30 μg ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined using a Pierce BCA protein assay kit (ThermoFisher Scientific) and 10 µg protein loaded into 15-well NuPage 4-12% Bis-Tris gels (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... and protein concentration determined using the Pierce BCA Protein Assay Kit (Thermo Fisher). A cellulose acetate membrane filter (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2023Quote: ... Total protein concentration was determined by a Pierce BCA Protein Assay Kit (ThermoFisher Scientific Inc. ...
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bioRxiv - Physiology 2022Quote: ... Protein abundance was normalized to total protein using Pierce™ Reversible Protein Stain Kit for PVDF Membranes (cat 24585; Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific; cat # 23227). Twenty or 30 µg samples were loaded into SDS-PAGE gels and transferred onto either Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Purity and integrity of all proteins were checked and protein concentrations estimated using quantitative densitometry on GelCode Blue-stained protein gels (Thermo Fisher Scientific) containing bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were kept and protein concentration was estimated using the micro-BCA Protein Assay Kit (Pierce BCA Protein Assay Kit (Thermo Scientific: 23225)) ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was measured using the Pierce 660nm Protein Assay Kit or the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Plant Biology 2019Quote: ... 2002) containing the 35S Cauliflower Mosaic virus promoter and eGFP gene using the Gateway® LR Clonase™ II kit (Invitrogen Corp., Carlsbad, CA). A single colony was used to amplify the [Gene-pK7FW2/pK7WGF2] plasmid and it was sequenced before transforming Agrobacterium (strain GV3101 ...
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bioRxiv - Microbiology 2019Quote: ... The number of JEV RNA copies present in BBB-crossing samples was determined by RT-qPCR using TaqMan® Fast Virus 1-Step Master Mix kit (Applied Biosystems®, 4444432) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and Mayaro virus (MAYV, TRVL 15537) were propagated in Aedes albopictus C6/36 cells grown in Minimum Essential Medium (MEM, Thermo Fisher Scientific, Waltham, MA). SARS-CoV-2 (SARS-CoV-2/CANADA/VIDO 01/2020 ...
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bioRxiv - Neuroscience 2021Quote: Images of whole brain slices for analysis of virus expression were obtained from a multichannel fluorescence microscope (EVOS FL Auto Imaging System, Thermo Fisher Scientific, Waltham, MA) with an automatic objective at 10X magnification ...
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bioRxiv - Microbiology 2021Quote: ... cDNA were synthesized from 100 pg of RNA and quantified using the TaqMan® Fast Virus 1-Step Master Mix (Thermofisher, Waltham, Massachusetts, USA) following manufacter’s instructions with appropriate oligonucleotides and Taqman probes (Table S1) ...
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bioRxiv - Immunology 2020Quote: Subgenomic RT-qPCR was performed on the QuantStudio™ 7 Flex Real-Time PCR System using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with forward primer ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was collected into 500 μL fractions and the virus containing fractions were detected using NanoDrop 1000 Spectrophotometer (Thermo Scientific, Waltham, Massachusetts, US). The virus containing sucrose fractions were diluted at least 1:5 in 2 mM MgCl2-PBS and the virus was pelleted by centrifugation (70Ti rotor ...
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bioRxiv - Microbiology 2023Quote: Cell culture supernatants or P1 virus cultures were mixed with a five-fold excess of TRIzol™ LS Reagent (Thermo Fisher Scientific, Waltham, MA). Viral RNAs were extracted by Direct-Zol™ RNA miniprep Plus Kit (ZYMO RESEARCH ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was subjected to one-step RTqPCR analysis as described using primers specific for the viral genome encoding for the RNA-dependent RNA polymerase (RdRp)23 and for E24 genes with TaqMan fast virus 1-step master mix (ThermoFisher Scientific, catalogue number 4444432) using AriaMx instrumentation (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... The infusion line was primed with a loading line solution that was identical to the virus suspension solution [Corning phosphate-buffered saline without Ca2+ or Mg2+ with 350 mM NaCl (Gibco, ThermoFisher Scientific, Waltham, MA) containing 0.001% F68 surfactant (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was directly subjected to one-step RT-qPCR analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA) and Bio-Rad CFX instrumentation (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 hrs protein-chromatin pull-down with a 1:1 mix of protein A and protein G Dynabeads (Thermo Fisher; 10001D and 10003D). ChIP samples were washed extensively ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA in the protein samples was sheared by sonication and the amount of protein was estimated using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were separated on 4–20% Mini-PROTEAN® TGX™ Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™, PI23227). The desirable amount of the protein lysate (15 – 30 μg ...
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bioRxiv - Microbiology 2022Quote: ... The EBOVLP input was normalized based on the concentration of EBOV structural proteins as determined with a bicinchoninic acid (BCA) protein assay (Pierce™ BCA protein assay kit, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014, respectively) for one hour ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified with a Micro BCA protein assay kit (Thermo Scientific, Rockford, IL) using the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein concentration was determined using the Bicinchoninic Acid protein assay (Thermo Scientific, US). Lysates (3μg/μl in 1xSDS ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was adjusted using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). For co-immunoprecipitation assay ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins separated by SDS-PAGE were stained with PageBlue Protein Staining Solution (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentrations were determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), before extracts were stored at −80°C ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were determined using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were estimated using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific# 23227). 400-500μg of protein from the stably transfected HEK293 cells were used to immunoprecipitate FLAG-tagged MIB1 using EZview™ Red ANTI-FLAG® M2 affinity gel (MilliporeSigma# F2426 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a Pierce BCA Protein Assay kit (Thermofisher Scientific, 23225) colorimetric reaction and quantified on a SpectraMax i3 Multi-Mode Platform (Molecular Devices) ...
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bioRxiv - Immunology 2021Quote: ... 40uL of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) were added to each well and then incubated at 4ºC for 4 hours on a rotator ...
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bioRxiv - Immunology 2021Quote: ... Total protein concentration was assessed using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of whole-cell protein lysates samples were boiled in Laemmli loading buffer ...
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bioRxiv - Genetics 2021Quote: ... Protein amounts were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein (in vitro samples ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined via the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted with Tissue Protein extraction reagent (T-PER, Thermofisher Scientific, USA) + Protease inhibitor cocktail (Pierce #A32961) ...
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bioRxiv - Neuroscience 2020Quote: ... Data were normalized to total protein concentration as determined by BCA protein assay (ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: ... protein concentration of lysates was measured using Pierce™ BCA Protein Assay Kit (ThermoFisher), to ensure equal loading of proteins ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts (100 µg of protein) were incubated with streptavidin agarose beads (Thermo Scientific) for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were measured using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). Cytoplasmic and nuclear samples from each brain region containing 5μg total protein was boiled in Laemmli loading buffer and run on a 4%–20% Bio-Rad precast gel ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein content was quantified with a Pierce BCA Protein Assay Kit (Thermo Scientific). The lysate [50 µg protein/50 µL] was incubated with 0.5 µL of 1 M DTT solution at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and the protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo Fisher). 50 μg of protein was loaded into each lane of Mini-Protean TGX gels (Biorad ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein amount was confirmed with a BCA protein assay kit (Thermo Fisher Scientific). Proteins were reduced by treatment with 10 mM DTT for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific), then 30 μg protein lysates were mixed with NuPAGE™ LDS Sample Buffer (4x ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Protein gels were stained with Coomassie blue (PageBlue Protein Staining Solution; Thermo Fisher Scientific).