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Citations for Thermo Fisher :
1901 - 1950 of 5129 citations for SPFH1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and blocked with Pierce™ SuperBlock™ T20 (TBS) Blocking Buffer (cat. no. 37581, Thermo Fisher) 0.5% Triton X-100 (cat ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by the addition of secondary antibodies in blocking buffer (goat-anti-rabbit-IgG-AlexaFluor488 [ThermoFisher Scientific #A11034] and goat-anti-mouse-IgG-AlexaFluor568 [ThermoFisher Scientific #A11031] both 1:200 ...
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bioRxiv - Microbiology 2023Quote: ... primary antibody and 488 (FLAG) Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer), then washed and imaged as described above.
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bioRxiv - Immunology 2023Quote: ... Samples were blocked for 1 hours with BlockAid blocking solution (Thermo Fisher Scientific, catalog no. B10710)) ...
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bioRxiv - Cancer Biology 2023Quote: ... specific sites were blocked with a blocking buffer solution (5% fetal bovine serum [FBS, Life Technologies, #10270-098] ...
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bioRxiv - Cell Biology 2023Quote: ... incubated for 1 hr in blocking buffer + secondary antibody (1:1000 donkey anti-rabbit alexa555 (Invitrogen), then washed 3 times for 5 min with TBST ...
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bioRxiv - Cell Biology 2023Quote: ... incubated for 1 hr in blocking buffer + secondary antibodies (1:1000 donkey anti-rabbit alexa555 (Invitrogen); goat anti-rabbit alexa488(Invitrogen)) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections then were incubated in blocking solution with chicken anti-GFP (1:1,000, Life Technologies, A10262) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were first incubated for blocking with 5% BSA (bovine serum albumin, BP1600-1 Fisher Scientific) in PBT (0.3% Triton X-100 (ACROS ...
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bioRxiv - Immunology 2023Quote: ... Wells were washed thrice with PBS and blocked with casein blocking buffer (Thermo Fisher Scientific, 37528) for 90 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: Secondary antibodies were diluted in blocking buffer at 1:10,000 anti-mouse-HRP (Thermo Fisher; 31340) and 1:10,000 anti-rabbit-HRP (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with secondary antibody in blocking buffer (Alexa conjugated antibodies, Thermo Fisher Scientific, 1:500) for 2 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... cells were first incubated for blocking with 5% BSA (bovine serum albumin, BP1600-1 Fisher Scientific) in PBT (0.3% Triton X-100 (ACROS ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were incubated with blocking solution (1% w/v BSA; BP1600-100, Fisher Scientific, in DPBS) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with secondary antibodies in blocking buffer (Alexa conjugated antibodies, Thermo Fisher Scientific, 1:500) for 2 h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Serum dilutions were prepared during the blocking step in a conical bottom plate (Thermo Scientific, 249570) in diluent buffer starting at 1:100 (1 μL serum into 99 μL diluent buffer ...
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bioRxiv - Physiology 2024Quote: ... then blocked for 15 minutes at room temperature in Superblock blocking buffer in PBS (Thermo Scientific) with 1% Triton™ X-100 (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... peptides were trapped using a 0.3×5 mm PepMap-100 C18 pre-column (Thermo Scientific) of 5 µm particle size and 100 Å pore size prior to separation on an analytical column (50 cm of length ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting peptides were analyzed by Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific). The peptides were subjected to ESI source followed by tandem mass spectrometry (MS/MS ...
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bioRxiv - Systems Biology 2020Quote: Peptides were injected to either a Q Exactive Plus Orbitrap (QE Plus, Thermo Fisher Scientific) or Orbitrap Fusion Lumos (FL ...
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bioRxiv - Cell Biology 2019Quote: ... Eluting peptides were electrosprayed into the mass spectrometer using a nanoelectrospray ion source (Thermo Scientific). An Active Background Ion Reduction Device (ESI Source Solutions ...
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bioRxiv - Cell Biology 2020Quote: ... The dried extracted peptides were resuspendedand analyzed by online nano-LC (Ultimate 3000, Thermo Scientific) directly linked to an impact IITM Hybrid Quadrupole Time of-Flight (QTOF ...
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bioRxiv - Microbiology 2019Quote: ... All peptide separations were carried out on an Easy-nLC 1000 nanosystem (Thermo Fisher Scientific). For each analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were directly eluted into an Orbitrap Exploris 480 instrument (Thermo Fisher Scientific, Bremen, Germany). Spray voltage were set to 1.8 kV ...
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bioRxiv - Developmental Biology 2022Quote: Digested peptides were analyzed in an Dionex UltiMate 3000 RSLC nano System (Thermo Fisher Scientific) coupled on-line to Orbitrap Fusion Lumos Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Peptides were electro-sprayed directly into an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Fisher Scientific) and analysed in Top Speed data-dependent mode ...
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bioRxiv - Immunology 2022Quote: ... peptides from the lung homogenates were labeled with a Tandem Mass Tag (TMT; Thermo Fisher) and mixed in equal proportions ...
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bioRxiv - Cancer Biology 2019Quote: ... The peptide mixture was gradient eluted into the Orbitrap Lumos Fusion mass spectrometer (Thermo Scientific) using the following gradient ...
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bioRxiv - Plant Biology 2019Quote: Eluted peptides were analyzed online on a Q Exactive Plus Orbitrap mass spectrometer (Thermo Scientific) operated with nanoESI in positive mode ...
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bioRxiv - Cell Biology 2019Quote: ... Peptides from each fraction were loaded onto a precolumn (4 cm POROS 10R2, Applied Biosystems) and eluted with an HPLC gradient (NanoAcquity UPLC system ...
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bioRxiv - Microbiology 2019Quote: Eluting peptides were analyzed with an Orbitrap Fusion Lumos (Thermo Fisher Scientific, Waltham, MA, USA). Survey scans were performed at R = 240,000 with wide isolation analysis of 300–1,350 mz ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were trapped onto a Acclaim PepMap 100 (Thermo Fisher Scientific Inc., Waltham, MA U.S.A.) precolumn ...
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bioRxiv - Genomics 2020Quote: ... The LC MSn data of digested peptides were collected on Orbitrap Fusion Tribrid (Thermo Scientific) with an on-line NanoLC system and analyzed using CID-MS2-MS3 strategy as previously described [62] ...
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bioRxiv - Biochemistry 2019Quote: ... The biotinylated peptides are retrieved by incubating the lysate with Dynabeads MyOne Streptavidin C1 (Invitrogen) 1h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... reconstituted peptides were separated using an online nanoflow EASY-nLC 1000 UHPLC system (ThermoFisher Scientific) and analyzed on a benchtop Orbitrap Q Exactive plus mass spectrometer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Eluting peptides were analyzed on a Q Exactive HF-X Orbitrap mass spectrometer (Thermo Fisher), which was coupled to the column with a customized nano-spray EASY-Spray ion-source (Thermo-Fisher ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were identified with tandem MS/MS experiments and a Proteome Discoverer 2.1 (Thermo Scientific) search ...
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bioRxiv - Biochemistry 2019Quote: ... Desalted peptides were eluted and directly analyzed by an Orbitrap Discovery mass spectrometer (Thermo Scientific). Peptides were identified with tandem MS/MS experiments and a Proteome Discoverer 2.1 (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Resulting peptides were separated on an acetonitrile gradient on the UltiMate 3000 HPLC (ThermoFisher Scientific). Eluted peptides passed through the AmaZon ETD ion trap and tandem mass spectrometer (Bruker) ...
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bioRxiv - Biochemistry 2019Quote: ... all the pre-fractionated peptides were analyzed with a nanoEasy LC (Thermo Scientific, Odense, Denmark) combined with an Orbitrap Fusion Tribrid™ Mass Spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were analyzed by Q ExactiveTM hybrid quadrupole - Orbitrap mass spectrometer (ThermoFisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, Thermo Scientific). The gradient was comprised of an increase from 6% to 20% solvent B (0.1% FA in 98% ACN ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were loaded onto an Acclaim PepMap 100 C18 HPLC column (Thermo Fisher Scientific 160454) in a 0.1% TFA mobile phase ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted into an Acclaim PepMap 100 C18 HPLC column (Thermo Fisher Scientific 164739) in a binary gradient between mobile phase A (99.9/0.1% v/v water/FA ...
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bioRxiv - Biochemistry 2020Quote: ... Digested AtMC4 peptides was used for mass spectrometry analysis using a Thermo QE-HF (ThermoFisher) at the Stony Brook University Biological Mass Spectrometry Shared Resource ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were sampled on a 300 μm x 5 mm PepMap C18 precolumn (Thermo Scientific) and separated on 75 μm x 150 mm and 75 μm x 250 mm C18 columns (Gemini C18 ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides were then analyzed by nano-LC-MS/MS on nano-LC-QExative Plus (ThermoFisher) in FTFT interfaced with the nano-Acquity LC system (Waters ...
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bioRxiv - Neuroscience 2021Quote: ... Enrichment for phosphorylated peptides was performed using Titanium dioxide beads (TiO2; kit: #432993; ThermoFisher Scientific) as described in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Peptides were separated by reverse phase HPLC (Thermo Easy nLC1000) (Thermo Fisher Scientific, Waltham, MA) using a precolumn (made in house ...
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bioRxiv - Neuroscience 2020Quote: ... 2 µg of cDNA encoding the desired peptides were transiently transfected by Lipofectamine 2000 (Invitrogen) for each confocal dish with a typical protocol according to the manual ...