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Citations for Thermo Fisher :
1901 - 1950 of 7736 citations for Recombinant Human TREM1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... For expression in eukaryotic cells the pcDNA™ 3.1/V5-His TOPO® TA Expression Kit (Invitrogen) was used according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Templating was performed using the Ion PI™ Hi-Q™ OT2 200 Kit (Thermo Fisher Scientific), followed by sequencing using the Ion PI™ Hi-Q™ Sequencing 200 Kit on Ion Proton PI™ V2 chips (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in Hi-Di formamide supplemented with R0X350 dye standards (1:8 ROXF) (Thermo Fisher Scientific). 1:3 and 1:15 dilutions of the samples in ROXF were analyzed by capillary electrophoresis (ELIM Biopharmaceuticals).
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bioRxiv - Cell Biology 2020Quote: ... the STIM1 cDNA was cloned into the pcDNA3.1/V5-His TOPO TA vector (Invitrogen, Cergy-Pontoise, France). All STIM1 variants were generated from the wild-type (WT ...
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bioRxiv - Cell Biology 2020Quote: ... His-ubiquitin conjugates were eluted by boiling resin in 2x LDS sample buffer (Thermo Fisher Scientific, NP0008) supplemented with 200 mM DTT and 200 mM imidazole HCl ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat ...
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bioRxiv - Biophysics 2021Quote: ... reaction with the PCR product and an XhoI/HindIII-digested pBAD/His-B vector (Thermo Fisher Scientific). Using pBAD-GCaMP6s as the template ...
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bioRxiv - Cell Biology 2022Quote: ... 250 µl of the clarified lysate was mixed with 150 µl HIS-Tag isolation dynabeads (Invitrogen 10103D) and 750 µl US buffer for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: Drosophila S2 cells were cultured at 23°C in Schneider’s media with 10% Gibco HI-FBS (Thermofisher) and 1% Gibco Antibiotic-Antimycotic (Thermofisher) ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific, Waltham, Massachusetts) for 20 minutes and then washed 5 x 1 minute in His-tag Isolation Buffer (100 mM Na-phosphate ...
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bioRxiv - Microbiology 2024Quote: ... Cells were grown in Ham’s F-12K medium (ATCC) with HI-FBS and 1x Antibiotic-Antimycotic (Gibco) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The entire pENTR effector library was then transferred into the pIB/V5-His vector (Invitrogen; Table S4) using LR Clonase II (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated overnight at 4°C with 100 ng of 6x His tag monoclonal antibodies (Invitrogen) in PBS and washed the next day ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were visualized using Ponceau stain and Western blot with monoclonal anti-His (Invitrogen MA1-21315), and polyclonal anti-GST (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen). Supernatants were applied to Strep-Tactin XT resin (IBA ...
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bioRxiv - Developmental Biology 2023Quote: ... Su(H) coding sequence was cloned from GH10914 (DGRC Stock 5973) into pAc5.1/V5-His A (Invitrogen) using the following primers ...
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bioRxiv - Biochemistry 2023Quote: ... the crude DEL sample was loaded in Novex™ Hi-Density TBE Sample Buffer (5X, Invitrogen LC6678), and electrophoresis was run at 12 W constant power ...
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bioRxiv - Immunology 2023Quote: ... using an Ion PI Hi-Q Sequencing 200 kit and Ion PI Chip v3 (Thermo Fisher Scientific). The FASTQ files were generated using AmpliSeqRNA plug-in v5.2.0.3 in the Torrent Suite software (v5.2.2 ...
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bioRxiv - Biochemistry 2023Quote: His-mATGL and its point mutants were recombinantly expressed in Expi293FTM cells (Thermo Fisher Scientific, Waltham, USA). The cells were cultivated in Expi Expression Medium (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... lysates were incubated for 1hour at 4 °C with His-Pure Cobalt Purification beads (Thermo Fisher Scientific). After incubation ...
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315 ...
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bioRxiv - Biochemistry 2024Quote: ... Rubrum CbbM coding region in a His-bdSUMO plasmid acquired from Addgene65 using OneShotTM Top10 cells (Invitrogen). The constructs were expressed in OneShotTMBL21-AITM (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... with Radiant Probe Hi-ROX qPCR Kit (Alkali Scientific) and Taqman Gene Expression Assays (Thermo Fisher Scientific). The expression of target genes was normalized to Gapdh expression.
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bioRxiv - Cell Biology 2020Quote: ... Recombinant baculovirus was generated using the Bac-to-Bac baculovirus expression system (Fisher Scientific) and was produced in Sf9 cells ...
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bioRxiv - Immunology 2021Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... linearized recombinant pCS2+ plasmids using the mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant antibody was labelled with Alexa Fluor 594 following the manufacturer’s instructions (Life technologies). Standard molecular biology techniques were used to introduce a cysteine residue at position 239 in the CH2 domain of each heavy chain (44) ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant baculovirus was produced using the Bac-to-Bac Baculovirus expression system (Thermo Fisher). The mouse utrophin (NP_035812 ...
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bioRxiv - Bioengineering 2021Quote: Recombinant baculovirus were generated by using a Bac-to-Bac expression system (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant protein was expressed in Expi 293 GnTI- (Thermo Fisher, Waltham, MA, USA) at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant cells were selected using 150 μg/ml of Hygromycin B (Invitrogen, Paisley, U.K.).
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bioRxiv - Cancer Biology 2022Quote: ... or by tryptic shaving of cell-surface proteins by using recombinant enzymes (TrypLE, Gibco). Three biological replicates of cells cultured in the presence or absence of serum ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant baculoviruses were generated according to the Bac-to-Bac Baculovirus expression system (Invitrogen). 2 ml of P3 virus were used to infect 1 L of Sf9 insect cells at a density of 1.1×106 ml-1 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant baculoviruses were generated through the Bac-to-Bac system (ActRIIA; Invitrogen - Waltham, MA) or the Baculogold system (ActRIIB ...
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bioRxiv - Cancer Biology 2021Quote: ... in the presence of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher, Cat. No. 10777019). Following 10 minutes ice-incubation and centrifugation at 21,000 x g for 1 minute ...
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bioRxiv - Microbiology 2021Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... in the presence of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher, Cat. No. 10777019). Following 10 minutes incubation on ice and centrifugation at 21,000 x g for 1 minute ...
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bioRxiv - Microbiology 2019Quote: ... Strains used for cloning and expression of recombinant proteins were Escherichia coli TOP10 (Invitrogen) and E ...
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bioRxiv - Immunology 2019Quote: ... Recombinant proteins were biotinylated with EZ-Link Sulfo NHS-LC-LC biotin (Thermo Fisher) at a 50 molar excess for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Purified recombinant proASP1 was crosslinked to 0.5 ml of AminoLink Coupling Resin (Thermo Fisher) and affinity purified anti-ASP1 antibodies were eluted with 0.2 M Glycine ...
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bioRxiv - Microbiology 2020Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Tau proteins were biotinylated with 5 molar excess of NHS-biotin conjugates (Thermofisher) during 4 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The recombinant eGFP reporter BAC was linearized using the restriction enzyme BsiWI (Thermo scientific) and purified using a CL4b sepharose column (Pharmacia) ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant baculovirus was generated using the Bac-to-Bac Baculovirus expression system (Invitrogen) and the proteins were expressed in Sf9 insect cells infected at a density of 1.0 x 106 ml−1 with P3 baculovirus stocks ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant hACE2-Fc protein was first biotinylated using EZ-Link Sulfo-NHS-Biotin (ThermoFisher) as the instruction described ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen, Carlsbad, CA, USA) and purified by nickel affinity chromatography ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Thermo Fisher Scientific). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... a recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...