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Citations for Thermo Fisher :
1901 - 1950 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibody incubations were for 1 hour at RT using fluorescently tagged antibodies from Invitrogen. All antibody dilutions were made in a solution of 1 mg/ml BSA in 0.25% Tween 20 in PBS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... For each sample pool we retained only biotin-tagged fragments using Dynabeads M-270 (Invitrogen), and performed six separate ...
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bioRxiv - Cancer Biology 2022Quote: Myc-tagged LCK containing plasmid was generated using pENTR/D-TOPO cloning kit (Thermo Scientific) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: Biotinylated proteins were tagged using streptavidin-coated quantum dots (Qdot 655 and Qdot 605 - ThermoFisher) by incubating at 100 nM in HSABC (50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Affinity separated FLAG-tagged protein was eluted using 35 μL IgG elution buffer (Thermo Fisher), denatured with equal volume of Laemlli buffer ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with FLAG-tagged OGA using Lipofectamine 3000 reagent (Thermo Fisher Scientific) at a ratio 1:2 (μg:μl ...
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bioRxiv - Cell Biology 2021Quote: Mouse hemagglutinin (HA) tagged Lphn2 construct was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) and cloned into the Gateway system pENTR221 vector (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... HA-tagged NPH3 was immunoprecipitated using Pierce™ Anti-HA Magnetic Beads (Thermo Fisher Scientific). Thiophosphorylation was visualised by immunoblotting with anti-thiophosphoester monoclonal antibody (clone 51-8 ...
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bioRxiv - Biochemistry 2022Quote: ... and transfected with pEGFP-C3 to express GFP-tagged S142A ExoU and RFP-Lamp1 (ThermoFisher), when 70% confluent ...
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bioRxiv - Plant Biology 2020Quote: ... Then 200-fold MPBS/MTCS-diluted secondary antibody AlexaFluor488-tagged donkey anti-rat IgG (Thermofisher) antibody for JIM5 and JIM7 ...
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bioRxiv - Neuroscience 2019Quote: ... 500 µg of proteins were used for c-Myc-tagged tomosyn co-immunoprecipitation (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... We immunoprecipitated GFP-tagged RBBP4 and RBBP7 proteins with anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each C-terminally HA tagged GC-C variant was synthesized via gene synthesis (Life Technologies). GC-C was then cloned into the lentiviral transfer vector pUltra (Addgene #24129 ...
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bioRxiv - Molecular Biology 2021Quote: Cells with tagged RPAC1 were grown in suspension in Expi293 Expression Medium (Thermo Fisher Scientific) up to 7 x 106 cells/mL ...
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bioRxiv - Biochemistry 2022Quote: ... TAP- or FTpA-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific) for 2.5 h at 20°C ...
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bioRxiv - Cell Biology 2022Quote: L6 rat myoblasts expressing myc-tagged GLUT4 (33) were maintained in α-MEM (12561056, Gibco) supplemented with 10% fetal bovine serum and 1% pen/strep antibiotic in a humidified atmosphere containing 5% CO2 and 95% air at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... or ALS-associated Ruby-tagged sequences of DnaJC7 using 5 μL of Lipofectamine 2000 (Gibco) transfection reagent in OptiMEM (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotin-tagged peptides were labeled with Pierce™ High Sensitivity NeutrAvidin™-HRP (Thermo Fisher) directly after blocking ...
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bioRxiv - Biochemistry 2024Quote: ... to assess FLAG-tagged protein expression and immunoprecipitation by Immunoblot and silver stain (ThermoFisher Scientific) respectively ...
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bioRxiv - Microbiology 2024Quote: ... Vectors of sulfatase 1 and 2 tagged with myc were transfected using Lipofectamine 2000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from clonal CRISPR tagged cells using Genomic DNA Mini Kit (Invitrogen) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-tagged cDNA constructs and siRNAs were transiently transfected using the Lipofectamine 3000 (Invitrogen, #L3000001) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... MBP-tagged protein was purified by affinity column chromatography using Ni-IMAC resin (Thermo Fisher) followed by size exclusion chromatography using a Superdex 200 column (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: Purified RRMs were fluorescently tagged using Alexa Fluor™ 633 C5 Maleimide (Thermo Fisher Scientific). The dye was added to a sample of purified protein (1:10 protein:dye molar ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotin-tagged ends were pulled down using Dynabeads MyOne Streptavidin C1 (Thermo Fisher Scientific 65001). Standard Illumina library preparation protocol including end repair ...
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bioRxiv - Genomics 2023Quote: ... Verification of a successful GST-tagged AP2 protein purification by GST-affinity purification (Thermo Scientific Pierce Glutathione Superflow Agarose beads ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary hybridization was performed using fluorescently tagged antibodies: AF647 goat anti-rabbit (A2l24S, Invitrogen, l:l000), AFS94 goat anti-rabbit (All037 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All ACE2-Fc constructs were produced in Expi293F cells (Thermo Fisher A14527) in Gibco Expi293 Expression Medium at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Genomics 2022Quote: ... and staining was performed in the presence of Fc block (eBioscience/ThermoFisher, True-Stain Monocyte Blocker (Biolegend) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were blocked with Fc block (CD16/32, Invitrogen, 14-0161-82) for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... and aliquots were incubated with Fc Block (ThermoFisher Scientific 14-9161-71) 20 minutes at 4°C ...
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bioRxiv - Immunology 2019Quote: ... containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific). Medium was exchanged after 3 hours and cells were stimulated.
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bioRxiv - Genomics 2020Quote: ... washed twice in FC buffer and resuspended in Sytox Blue (Thermo Scientific) containing FC buffer for live/dead discrimination according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% penicillin-streptomycin and 10% fetal calf serum (FCS, Gibco) in an incubator (37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Monocytes were immersed in 45% fetal calf serum (FCS, 10500 ThermoFisher Scientific), and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS, Thermo Fisher Scientific) and 1% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... LAIR-1 Fc was biotinylated with EZ-Link NHS-PEG4-Biotin (ThermoFisher), No-Weight Format (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... FCS and L-Glutamine were all purchased from Gibco (Thermo Fisher Scientific). The following chemicals were obtained from Roth ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% FCS (Eurobio) and 1% Non-Essential Amino Acids (Gibco). Primary astrocytes were obtained from E17 rat embryos following (Etienne-Manneville ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum (FCS; ThermoFisher, 10499044 [Lot Number 08G8293K]) at 37°C with 5% CO2 ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... filtered through 0.1um spin filter with 20uL/sample of Fc block (ThermoFisher) for 30min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... in MLNL medium (low-glucose DMEM/1% FCS, Thermo Fisher Scientific 21885025). At day 2 ...
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bioRxiv - Physiology 2019Quote: ... On day 0 media was additionally supplemented with 10% FCS (Gibco#10270106). The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% v/v (FCS) and 2 mM L-glutamine (Gibco). Where indicated ...
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bioRxiv - Immunology 2020Quote: ... and optical densities were acquired with a Multiskan™FC (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... after which staining was inhibited by adding 10% FCS (10270-106, Gibco). Stained cells were washed in RPMI/PS prior to counting and addition to macrophages ...
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bioRxiv - Molecular Biology 2021Quote: ... These cells were cultured in DMEM containing 10% FCS (Thermo Fisher Scientific) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Absorbance was measured at 405 nm using a Multiskan FC (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific) at medium shaking speed ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% FCS and 1% penicillin/streptomycin (#P433, Thermo Fisher Scientific) at 37°C and 5% CO2 ...