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Citations for Thermo Fisher :
1901 - 1950 of 8309 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor555-conjugated goat anti-rat IgG (A-11007, Invitrogen, 1:200 dilution) or Alexa Fluor 488-conjugated goat anti-rabbit IgG (A-11037 ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-rat IgG Alexa Fluor Plus 647 (1:2,000, Thermo Fisher Scientific). After the secondary antibody reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Appropriate secondary antibodies were used (1:500, goat anti-rat Alexa 488 Invitrogen A11006 ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-B-lymphocyte activation antigen B7-2 (CD86, Invitrogen, MA1-10299, RRID:AB_11152324), guinea pig anti-vesicular glutamate transporter 1 (vGlut1 ...
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bioRxiv - Neuroscience 2024Quote: ... and rat anti-mCherry antiserum (1:2500; Thermo Fisher, Product#: M11217, Lot#: 2695213). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-rat CD73 (Fisher Scientific, BDB551123, 5 μl per 10^7 cells) and mouse IgG microbeads (Miltenyi Biotech ...
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bioRxiv - Bioengineering 2024Quote: ... rat anti-TER-119 antibody (1:500; Invitrogen, Cat No. 14-5921-82), recombinant rabbit anti-IBA-1 (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... AlexaFluor555 (anti-mouse) and AlexaFluor488 (anti-rat, both secondary antibodies were from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 488 goat anti-rat (1:400, Thermo Fisher Scientific, RRID: AB_2534074), 488 anti-HA DyLight™ (1:400 ...
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bioRxiv - Immunology 2024Quote: ... and Alexa561 conjugated rat anti-F4/80 (Invitrogen #505-4801-80, 1:100), respectively with the secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... The plasmids were co-transfected with packaging plasmids (pspAX2 and pMD2G) into 293T cells using Turbofect (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were sequenced and transfected along with lentiviral packaging plasmids into HEK293FT cells with Lipofectamine 3000 (Thermo Fisher) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The cloned plasmids were then isolated from their bacterial cultures using GeneJET Plasmid Miniprep Kit from Thermo Fisher scientific (catalog number-K0503).
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bioRxiv - Immunology 2021Quote: ... Plasmid of positive clones were extracted using the GeneJET Plasmid Miniprep Kit following the manufacture protocol (ThermoFisher scientific). and tThe nucleocapsid cloning was confirmed by digestion with using BamHI and NheI FastDigest enzymes (ThermoFisher Scientific) ...
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: ... Plasmids were extracted with Favorprep Plasmid Extraction Mini Kit and cleaved with FastDigest SacI restriction enzyme (Thermo Scientific) to eliminate the toxin plasmids ...
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bioRxiv - Immunology 2022Quote: ... heavy chain plasmid DNA and light chain plasmid DNA were diluted in 1.5ml Opti-Plex Complexation Buffer (Invitrogen) before mixing with 80µl ExpiFectamine 293 Reagent (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... and total DNA amounts of 5μg (2.5μg donor vector plus 1.25μg of each TALENS arm plasmid) or 2μg (1μg piggybac vector plus 1μg of transposase plasmid) were combined with Lipofectamine Stem (ThermoFisher, STEM00003) in Opti-MEM (ThermoFisher 11058021) ...
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bioRxiv - Neuroscience 2020Quote: ... Large quantities of purified plasmids were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep kit (Thermo Fisher Scientific). The cloned sequences of ItypOrco ...
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bioRxiv - Immunology 2019Quote: ... plasmids were treated with DpnI to remove non-mutated parental plasmids and cloned into Stbl2 cells (Life Technologies) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... 5 × 104 E14Tg2a cells were transfected with 100 ng of reporter plasmids (pGL4.10[luc2] and their derivatives) and 1 ng of pGL4.70[hRluc] control plasmid using Lipofectamine 2000 (Invitrogen) in 96-well plates ...
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bioRxiv - Biophysics 2019Quote: ... were selected for their suitability for transformation with a plasmid containing ampicillin resistance (pRSET/EmGFP plasmid; Invitrogen, UK).
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bioRxiv - Neuroscience 2019Quote: ... human Ad5 genomic plasmid (pBHGfrtΔE1,3FLP; Microbix) and each pDC511-LRRK2 shuttle plasmid were introduced by calcium phosphate (Invitrogen) transfection into stable Tet-On-inducible E2a cells (E2T cells ...
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bioRxiv - Cancer Biology 2020Quote: ... the lentiviral plasmids and packaging plasmids were transfected into lentiX-293T cells using Lipofectamine 2000 (Invitrogen, Carlsbud, CA) and lentivirus-containing supernatants were harvested after 3 days ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were then extracted and purified using the PureLink HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific, Cat# K210006) for downstream experiments ...
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bioRxiv - Microbiology 2024Quote: Isolation of plasmid DNA was carried out with Genejet Plasmid Miniprep kit (Thermo Fisher Scientific, Pittsburgh, PA, USA), according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 750 ng of plasmid purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA was isolated using the GeneJET Plasmid Miniprep Kit (Cat #K0503; Thermo Fisher Scientific, MA, The US). Isolated plasmids were prepared for transformation by linearizing with EcoRI restriction enzyme according to the supplier’s instructions (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The confirmed plasmid was prepared in large quantities using the PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific). The plasmid concentration was measured using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng firefly luciferase plasmid and 112.5 ng GFP or different eIF1 plasmids using Lipofectamine 2000 (Thermo Fisher). After 24 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 µL of plasmid-lipofectamine mixture containing 75 ng plasmid and 165 ng Lipofectamine 2000 (Invitrogen, 11668-019) in Opti-MEM (Gibco ...
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bioRxiv - Genomics 2023Quote: ... Colonies were selected and plasmid DNA was extracted using The PureLink™ Quick Plasmid Miniprep Kit (Thermofisher Scientific) and sent for Sanger sequencing to identify the integrity and quality of the inserted enhancer fragment ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid transfections were performed using 2 μg plasmid per 3.5-cm well plates using Lipofectamine 2000 (Life Technologies) transfection reagent according to the manufacturer’s recommendations at 48 hours before harvest ...
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bioRxiv - Systems Biology 2023Quote: ... coli (High Efficiency) cells and the plasmid DNA was purified using the PureLink™ Plasmid Purification Kit (Invitrogen). Three oligonucleotide pools were cloned separately and pooled together to acquire the final 7mer library ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmids containing the promoter regions were ordered via gene synthesis as Gateway-compatible donor plasmids (ThermoFisher Scientific). Promoter sequence and expression cassette from donor constructs pEntry.P4P1R.EF1a and pEntry.MiniCas9V2 (Maria et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the pWS082-CAN1 sgRNA plasmid (Table S1) was extracted using the GeneJet Plasmid miniprep kit (ThermoFisher Scientific). Accuracy of the cloning was confirmed through DNA sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... The BUB1 plasmids or siRNA with plasmids were transfected with Lipofectamine 2000 (Catalog No. 11668500, Thermo Fisher Scientific) per manufacture’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... using PureLink Quick Plasmid Miniprep Kit (Invitrogen) and were sequence verified ...
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bioRxiv - Synthetic Biology 2021Quote: ... 500ng of T7 calmodulin plasmid (Invitrogen, #454002) was also used in the following cell free expression reactions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA and Plus reagent (Invitrogen # 11514015) were mixed at 1:1 and incubated for 5 minutes ...
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bioRxiv - Biophysics 2020Quote: ... and PureLink HiPure Plasmid Midiprep Kit (Invitrogen). The DNA sequences were read by dye terminator cycle sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... and cloned into the pBAD plasmid (ThermoFisher), as previously described (46) ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids were transfected using Lipofectamin 2000 (Invitrogen) according to the manufacturer’s instructions for 48 hours before harvesting ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids were delivered using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmids by Lipofectamine 3000 (Invitrogen, L3000015, USA). The relative hACE2 quantity was evaluated through mRNA expression level using following primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and cloned into the pCR8 plasmid (Invitrogen), then transferred using the Gateway system into the pBPGUw (Pfeiffer et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids were transformed into BL21(DE3)(Invitrogen). Bacterial cultures were grown to an OD600 of 0.6 at 37 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... later sub-cloned in pet28a plasmid (Invitrogen) in order to produce 6 His-tagged proteins.
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bioRxiv - Bioengineering 2019Quote: ... derived from plasmid pYES1L (Thermo Fisher Scientific), was inserted into pRM01 ...
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bioRxiv - Immunology 2019Quote: ... into a modified pMT/BiP plasmid (Invitrogen) that encodes for a C-terminal strep-tag sequence ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmid mixes were prepared in Optimem (Gibco) and mixed with 0.75 μl of LF2000 and added dropwise to cells ...