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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed three times with PBS and then incubated with appropriate secondary antibodies (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed after cell fixation with 4% para-formaldehyde (PFA) by incubating the cells with conjugated antibodies in permeabilization buffer (Thermo Fisher Scientific). For the phenotyping of DN subsets ...
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bioRxiv - Molecular Biology 2023Quote: ... Activation was performed by seeding cells at 105 cells/ml in culture medium supplemented with 1 μg/ml anti-CD40 antibody (Invitrogen, MA1-81395), 5 ng/ml IL-4 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... residual tumor cells and T cells were quantified by FACS combining antibody staining and absolute counting beads (CountBright Beads, Life Technologies #C36950). Fresh tumor cells were added back to replicate wells when >85% of tumor cells were killed at the analysis timepoint ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with antibody cocktail (Supplementary Table 7) along with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen; cat # L34957) or eBioscience Fixable eFluor780 viability dye (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Re-suspend cells in 100 μl of diluted secondary antibody (in this case Alexa Fluor 488 donkey anti-rabbit antibody, ThermoFisher Scientific A21206, diluted 1:1000) in 1% BSA in PBS with 1:25 RNasin Plus ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were detected by incubating cells for 30 minutes with AlexaFluor conjugated secondary antibodies made in goat (ThermoFisher, 1:200 in PBS + 10% NGS). Coverslips were mounted onto microscopy slides (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated overnight at 4 °C with primary antibodies: rabbit anti-phospho-EGFR (Y845) polyclonal antibody (44-784G, Thermo Fisher Scientific, diluted 1:200); Purified Mouse anti-E-Cadherin monoclonal antibody (Clone 36 ...
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bioRxiv - Immunology 2020Quote: ... Tetramers were prepared in house by mixing biotinylated (NANP)9 peptide with streptavidin conjugated PE or APC (Invitrogen) in a 4:1 molar ratio ...
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bioRxiv - Biochemistry 2021Quote: ... labeled with 50 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher Scientific Invitrogen Catalog # 12-4998-82) diluted in 1.95 mL TBSF for 10 minutes covered on ice ...
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bioRxiv - Immunology 2020Quote: To this aim the stabilized Spike protein was coupled to Streptavidin-PE (eBioscience # 12-4317-87, Thermo Fisher) for 30 min at 4°C and then incubated with VERO E6 cells ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
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bioRxiv - Immunology 2021Quote: ... followed by intracellular staining with Foxp3-PE/eFluor610 (clone FJK-16s, Thermo Fisher 61-5773-82, 1:200) or Ki67-eFluor450 and analysis on a 4 laser Cytoflex S instrument (Beckmann Coulter) ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: Quantitative RT-PCR was performed in duplo on an ABI Prism 7900HT Sequence Detection System (PE Applied Biosystems) or StepOnePlus Real-Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... To this aim the stabilized Spike protein was coupled to Streptavidin-PE (eBioscience # 12-4317-87, Thermo Fisher) for 1h at room temperature and then incubated with VERO E6 cells ...
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bioRxiv - Immunology 2021Quote: ... The following day the cells were stained with antibodies against extracellular markers (MHC-II eFluor450, eBioscience 48-5321-82; CD4 BV785, bioLegend 100552; NK1.1 PerCP-Cy5.5, BD Biosciences 561111; CD11b PE, Invitrogen 12-0112-82 ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Culture supernatants were filtered through 0.2 μm Nalgene Rapid-Flow Bottle Top Filters with PES membranes (Thermo Scientific) and concentrated using 15 mL ...
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bioRxiv - Immunology 2024Quote: ... CD206-FITC (19.2, 1:100), CD11c-PE (3.9, 1:50) and CD14-PerCP-Cy5.5 (61D3, 1:200) from eBiosciences (ThermoFisher) were used.
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bioRxiv - Microbiology 2024Quote: ... followed by vacuum filtration using a 0.45 µm filter and 0.22 µm filter with PES membranes (ThermoFisher Scientific). Supernatants were concentrated using Centricon Plus-70 centrifugal filter units with a membrane pore size of 100,000 Da (EMD Millipore ...
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bioRxiv - Biophysics 2023Quote: ... these samples were concentrated using a Pierce PES protein concentrator with appropriate molecular weight cutoff (MWCO, ThermoFisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Probes were freshly prepared for each experiment by incubation of mono-biotinylated RH5ΔNC with streptavidin-PE (S866, Invitrogen) or streptavidin-APC (405207 ...
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bioRxiv - Developmental Biology 2022Quote: ... together with its IgG2a kappa PE/Cyanine 7-Isotype control (1:500, Thermo Fisher Scientific, 25-4321-81), CD31-APC with human specificity for HUVEC experiments (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... the proteins in the medium were concentrated using Pierce™ Protein Concentrator PES tubes (Thermo Fisher Scientific: 88517). Protein concentration was determined by Bradford assay ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins contained in the medium were concentrated using Pierce™ Protein Concentrator PES tubes (Thermo Fisher Scientific: 88517).
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bioRxiv - Neuroscience 2024Quote: ... The reactions were run in an ABI PRISM 7000 sequence detection system (PE Applied Biosystems, Foster City, California). Alternatively ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were filtered through a 0.22 µm filter unit (Nalgene sterile disposable filter with PES membranes, ThermoFisher Scientific). Nickel Sepharose excel (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... The reactions were run in an ABI PRISM 7000 sequence detection system (PE Applied Biosystems, Foster City, California), or a 10 µM cDNA template was used in a 10 µl system in 384-well plates with SYBR Green qPCR SuperMix/ROX (Smart Bioscience Inc ...
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bioRxiv - Genomics 2024Quote: ... The lentivirus was then pooled and filtered through 500mL 0.45μm PES filtration units (ThermoFisher, catalog no. 166-0045) to remove any cellular debris ...
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bioRxiv - Cell Biology 2020Quote: ... h-iECs were dissociated into single cells and sorted into CD31+ and CD31-cells using magnetic beads coated with anti-human CD31 antibodies (DynaBeads, ThermoFisher, Cat No. 11155D). The purified CD31+ h-iECs were then expanded in culture on 10-cm dishes coated with 1% gelatin ...
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bioRxiv - Cancer Biology 2023Quote: ... Total viable cells were sorted on BD FACS Symphony S6 using following antibodies: epithelial cells – CD326 PerCP-eFluor710 (ThermoFisher, catalog #46-5791-82); CD45+ immune cells – CD45-PE (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: ... Cells were labeled with primary antibodies using a 1:100 dilution of Anti-mouse CD31 antibody conjugated to FITC (Thermo Fisher Scientific, Cat#: 11-0311-81), followed by a 1:100 dilution of Anti-mouse CD45 antibody conjugated to FITC (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... Figure S5 shows the knockdown efficiency of siRNA transfections in each cell line with GAPDH siRNA compared to no siRNA (just N-TER transfection reagent) and negative control siRNA by Western blot (GAPDH antibody: Cell Signaling Technology, Cat#2118; Tubulin antibody: Thermo Fisher Scientific, Cat#62204). Experiments were performed 24hr after transfection.
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were then incubated with fluorescently-labeled secondary antibodies and NeuroTrace Blue Fluorescent Nissl Stain (Invitrogen) in MAXStain Staining medium ...
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MYB insufficiency disrupts proteostasis in hematopoietic stem cells leading to age-related neoplasiabioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with eFluor 450-conjugated anti-Ki67 antibody (clone SolA15, ThermoFisher, Waltham MA) and then analysed by flow cytometry.
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bioRxiv - Cell Biology 2020Quote: ... Labelled cells were stained with Alexa-Fluor™ 594 anti-mouse secondary antibody (1:1000, Invitrogen) for 1 hour at RT and counterstained with VECTASHIELD antifade mounting medium with DAPI (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with Alexa Fluor 568-conjugated anti-mouse secondary antibodies (Invitrogen, Molecular Probes) and stained with DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were incubated with a 1:200 dilution of human cTnT primary mouse antibody (ThermoFisher) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed three times with PBS and incubated in secondary antibodies (1:200, Life Technologies) and phalloidin (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with the primary antibody solution (1:100 dilution; PDGFRA-APC, Thermo Fisher, cat#17-1401-81 ...
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bioRxiv - Immunology 2021Quote: ... The NK cells in the tumors were stained using the CD56 monoclonal antibody (clone 56C04, ThermoFisher) at a 1:200 dilution ...
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bioRxiv - Genetics 2021Quote: ... fixed cells were incubated with 1 µg/ml mouse anti-BrdU antibody (Fisher Scientific; BD Pharmingen) at room temperature for 2 hours and incubated with donkey anti-mouse-Alexa 488 (Invitrogen Molecular Probes ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with anti-mouse secondary antibody (conjugated to Alexa 594 or Alexa 488, Invitrogen), diluted 1:1000 in PBS-BSA 3% ...
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bioRxiv - Developmental Biology 2020Quote: ... The completion of differentiation was confirmed by staining cells with MAP2-antibody (PA5-17646, Invitrogen, U.S.) that are specific for mature neurons.
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bioRxiv - Microbiology 2022Quote: Monoclonal antibodies and engineered ACE2 were expressed using the Expi293F cell expression system (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed and labeled with donkey anti-mouse AF488 secondary antibody (Invitrogen, A11029; 1:1000) in 1% BSA in PBS with 0.1% NP-40 for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Stimulation of T-cell proliferation was achieved by incubation with CD3/CD28 antibody beads (Thermo Fisher). Carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated in secondary antibodies (donkey anti-rabbit 488 (Life Technologies A-21206 1:500)) for 30 min at room temperature in 0.2% BSA and 0.1% Triton X-100 in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were stained for goat anti-rabbit secondary antibody Alexa flour 488 (Invitrogen,1:500) for 20 minutes on ice in the dark ...
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bioRxiv - Immunology 2021Quote: Recombinant antibodies were transiently transfected and produced in vitro in 293F Freestyle suspension cells (Thermofisher Scientific). Briefly ...