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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Measurement of final protein concentration was performed using BCA protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™, #23227), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentration was determined using a bicinchoninic acid (BCA) assay from the Pierce™ BCA Protein Assay Kit (Thermo Scientific). The fluorescence spectra of the EYFP samples were measured with a Varioskan LUX Multimode Microplate Reader (Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: Triglyceride content of samples was measured using a GPO-PAP Triglyceride assay (Randox Laboratories Ltd) and cholesterol using Amplex Red Cholesterol Assay Kit (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Total protein amounts of the OM cell lysates were quantified with BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared by adding standard radioimmunoprecipitation assay (RIPA) buffer and normalized using a BCA protein assay kit (Thermo Scientific). Proteins were separated by sodiumdodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2022Quote: ... and protein concentration for each sample was determined using a BCA assay (Pierce Microplate BCA Protein Assay Kit, ThermoFisher, Waltham, MA). Equal amounts of protein were prepared using 4x NuPage sample buffer (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... The purified proteins and protein standards were subjected to a colorimetric bicinchoninic acid (BCA) assay following the manufacturer’s protocol and then stored at −80°C until use (Pierce protein assay kit; ThermoFisher Scientific, USA). The proteins were visualized by running a 4-20% SDS-PAGE (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: The OPP assay was performed using the Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The protein concentration of the supernatant was determined by bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific). 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K ...
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bioRxiv - Biochemistry 2023Quote: The inhibitory effect of a panel of four small-molecule inhibitors was determined using a modification of the amylase activity assay in CVLs used above (EnzCheck Ultra Amylase Assay Kit ThermoFisher, E33651). For initial screening ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured by the bicinchoninic acid (BCA) assay using the Pierce BCA protein assay kit (ThermoFisher Scientific: 23225). Equal amounts of protein were loaded on 4 to 12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: ... The collected supernatant was used to measure protein concentration using the BCA assay (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was used to determine the protein concentration by BCA assay according to the manufacturer’s instructions (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; ThermoFisher Scientific; cat # 23227). Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences ...
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bioRxiv - Molecular Biology 2023Quote: ROS assay was performed using a Total Reactive Oxygen Species Assay Kit 520nM (Cat. Nr. 88-5930-74, Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration in supernatant was determined using bicinchoninic acid (BCA) protein assay (PierceTM BCA Protein Assay Kit, Catalog #23225, ThermoFisher Scientific). 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: EdU assays were performed following the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher, C10634) manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... Pierce™ Quantitative Colorimetric Peptide Assay (23275) and Pierce™ Rapid Gold BCA Protein Assay Kit (A53227) were ordered from ThermoFisher Scientific (Waltham ...
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bioRxiv - Physiology 2024Quote: ... The protein content of the EV preparations was quantified by bicinchoninic acid protein assay (Pierce™ BCA Protein Assay Kit, Thermofisher).
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bioRxiv - Genomics 2020Quote: ... Serum chemistry analysis for ALT and AST levels were performed using commercial assay kits (Infinity Kits, Thermo Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: Plasma lipids were determined enzymatically using commercially available kits: triglycerides (Infinity Triglyceride colorimetric assay kit (Thermo Fisher, TR22421), non-esterified free fatty acids (WAKO) ...
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bioRxiv - Microbiology 2021Quote: ... Sterol content was measured using the quantitative fluorometric kit ‘Amplex Red Sterol Assay Kit’ (Molecular Probes, Life Technologies). Protein content was measured using the BCA kit (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... Sterol content was measured using the quantitative fluorometric kit ‘Amplex Red Sterol Assay Kit’ (Molecular Probes, Life Technologies). Protein content was measured using the BCA kit (Pierce ...
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bioRxiv - Physiology 2021Quote: ... The concentrations of TG were measured using a triglyceride assay kit (Infinity™ triglycerides kit, Thermo Fisher Scientific) and normalized to tissue weights as previously described13.
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bioRxiv - Cancer Biology 2024Quote: ... Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection kit was used (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... Final sEVs pellet was resuspended in 100 μL of PBS and the protein content was measured by bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). The NS500 nanoparticle characterization system (NanoSight ...
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bioRxiv - Microbiology 2019Quote: ... Nucleic acid concentrations were assessed using Qubit dsDNA HS Assay and Qubit RNA HS Assay Kits (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was collected and the concentration of proteins was measured by BCA(bicinchoninic acid) assay using bovine serum albumin (BSA) as a standard (BCA Protein Assay Kit, Thermo Fisher, 23227). Sample was mixed in loading buffer (P1016 ...
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bioRxiv - Molecular Biology 2019Quote: ... the absence of DNA contamination and the RNA concentration in the specimens were determined fluorometrically with Qubit™ dsDNA BR Assay and Qubit™ RNA BR Assay Kits (Invitrogen), respectively ...
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bioRxiv - Pathology 2019Quote: ... Protein concentration of the supernatant was determined using the BCA assay (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific, cat. number 23225). Samples were mixed with SDS-loading buffer without heating and electrophoretically separated on a 4–20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-rad ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was removed and the protein concentration was measured by Bradford assay (Pierce Coomassie Plus Assay kit, Thermo Scientific, Catalog #23236). 25µL of DYNAbeads Protein G (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were transferred to new ice-cold Eppendorf tubes and protein concentration was measured by the BCA assay using Pierce BCA Protein Assay kit (ThermoFisher Scientific; #23225). Equal amounts of protein were loaded on 4-12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Neuroscience 2021Quote: ... RIPA soluble supernatants were quantified for protein concentration using the BCA assay according to the manufacturer’s protocol (Pierce® BCA assay kit, Thermo Fisher, 23225). RIPA soluble samples (30 μg ...
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bioRxiv - Microbiology 2022Quote: ... the quantity and quality of the samples was tested using standard spectroscopic measurements (Nanodrop One) and using the Qubit 1X dsDNA HS assay and Qubit RNA HS assay kit (Thermo Fisher Scientific). Finally ...
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bioRxiv - Plant Biology 2019Quote: ... The protein concentration of the clear supernatant was determined using the bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific Pierce).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The protein content of supernatants was measured using a BiCinchoninic acid Assay (BCA) protein assay kit (Thremo Fisher Scientific, Mississauga, ON, Canada). Equal amounts of protein in lysis buffer (10 µg ...
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bioRxiv - Microbiology 2019Quote: ... Extract concentrations were measured with the Quant-iT RNA HS assay and the Quant-iT dsDNA HS assay kits together with the Qubit fluorometer (Invitrogen, Lidingö, Sweden). The RNA and DNA extraction yields ranged 2.5 – 122 ng μl−1 and 0.5 – 600 ng μl−1 ...
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bioRxiv - Developmental Biology 2021Quote: ... and translation (HPG) assays were carried out as described previously(Percharde et al. 2018) using Click-iT Assay Kits (Thermo Fisher Scientific) and according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations of the supernatants were determined via the bicinchoninic acid assay (BCA) using the Pierce BCA Protein Assay Kit (catalog number 23225, Thermo Fisher Scientific). Following BCA-based protein concentration measurements ...
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bioRxiv - Microbiology 2022Quote: Cell-cycle progression was evaluated using a previously published [10] EdU pulse-chase assay using the Click-iT™ plus EdU AF-647 flow cytometry assay kit (ThermoFisher Scientific). Five hundred thousand cells from d2EGFP TBX4B and d2EGFP TBW 11.50 were cultured in complete growth medium in a 48-well plate (Corning) ...
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bioRxiv - Neuroscience 2024Quote: Cellular cytotoxicity assay has been performed by measuring Lactate dehydrogenase (LDH) in the media by CyQUANT LDH Cytotoxicity Assay kit (Thermo Fisher Scientific), following the manufacturer instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Protein content of the crude mitochondrial fraction was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific catalog number 23225). Thirty µg of mitochondria protein was incubated at 25 °C in 850 µL reaction buffer (100 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... mRNA-LNPs were evaluated for encapsulation efficiency and mRNA concentration using RiboGreen assay using the Quant-iT RiboGreen RNA Assay Kit (Catalog # R11490, Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Systems Biology 2023Quote: ... the concentration of glucose produced from the glycogen was evaluated by using the Amplex Red Glucose/Glucose Oxidase Assay Kit glucose assay (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: Assay was performed on EBs and hPSCs using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Thermo Fisher Scientific). EBs used for the EdU cell proliferation assay were generated and maintained for 5 days using the same protocol as described31,77 ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific; cat # 23227). Twenty or 30 µg samples were loaded into SDS-PAGE gels and transferred onto either Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... Pure EVs were used for western blot characterization and infection using fractions standardized to the same amount of protein (TPS) determined by the BCA assay (Pierce BCA assay kit, Thermo Fisher Scientific). The graphical scheme of isolation is shown in Figure 1.
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were removed and the protein concentration was quantified using the colorimetric assay by PierceTM BCA protein assay kit (ThermoFisher Scientific, 23225). Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations for the extracellular solution and cell lysate supernatant were determined by BCA assay with Pierce BCA protein assay kit (Thermo Fisher Scientific) according to manufacture protocol on Synergy H1 microplate reader (Biotek) ...