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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 7 Oxabicyclo 4.1.0 hepta 2 4 diene 1 3 4 trifluoro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... the cell cultures were washed 3 times with 37 °C DPBS+ and fixed using 4% w/v paraformaldehyde (PFA; Affymetrix) in PBS for 2.5 h ...
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bioRxiv - Genetics 2022Quote: ... LC-MS/MS based proteomics datasets (4 groups × 3 replicates) were then generated in a lab (code: NVG) using Q Exactive HF-X mass spectrometer (ThermoFisher). To eliminate confounding factors such as experimental sample-processing order with sample group ...
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bioRxiv - Neuroscience 2022Quote: ... slices for optical recordings were incubated for 30 minutes in oxygenated Krebs solution added to a 3% Di-4-ANEPPS (Invitrogen) stock solution and mixed with an equal volume of fetal bovine serum (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... mRNA samples of 4 ng were subjected to DNaseI treatment and 3’ dephosphorylation using FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) and T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The enteroids were then pelleted at 300 x g for 3 min at 4 °C and washed once with advanced DMEM/F-12 basal media (Gibco). The enteroid pellet was resuspended in the desired volume of IntestiCult complete medium (Stemcell Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred to a 15 mL column containing 3 mL Ni-NTA Agarose affinity resin equilibrated with buffer A at 4 °C (Invitrogen). The column was washed using a gradient of Buffer A containing 20 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Microbiology 2023Quote: ... was used for induction of gene expression and X-gal (X-Gal 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside; Thermofisher) TSA plates were used for bacterial assessment ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... For differential glucose concentration treatments between day 2 and 3 and between day 3 and 4, N2B27 was made using DMEM/F12 without glucose (L0091-500, Biowest) and Neurobasal without glucose (A2477501, ThermoFisher). For 1X and 3X glucose concentrations ...
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bioRxiv - Immunology 2023Quote: Following euthanasia, adult zebrafish heads (15 wpf, N=3) were fixed in a solution of 4% methanol-free formaldehyde (Thermofisher) in 60 mM HEPES buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell bodies were removed by centrifugation (1,150 g, 3 min, 4°C; Sorvall Legend XTR, Thermo Fisher Scientific). A sucrose cushion (10 ML of 25% sucrose in HMS ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% CO2 in a humidified incubator and were passaged every 3-4 days using 0.05% Trypsin-EDTA (ThermoFisher Scientific, 25300). Stable U2OS-derived cell lines ...
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bioRxiv - Immunology 2023Quote: ... The cells were then blocked at 4℃ for 15 mins in 200 µl CyFACS buffer containing 3 µl rat serum (Invitrogen), 3 µl mouse serum (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were treated with either DMSO or 5 μM GolgiTracker ((BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine)) (Invitrogen. Cat# D3521) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2023Quote: ... 16 mL of HMA-10 buffer was added and the preparation was put on a shaking rocker for one hour at 4 °C with 3 units/mL Rnase-free Dnase (Ambion) added ...
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bioRxiv - Cancer Biology 2024Quote: ... then equal quantities of protein lysate (3-10 µg) were separated by SDS-PAGE using 4–12% gradient gels (Invitrogen) and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2024Quote: ... islets from 3-4-week-old control and β-LonP1KO mice were handpicked and then dispersed with trypsin (Thermo Scientific). Islet cells were counted and incubated with adenovirus expressing an empty vector control (Ad-RIP2 EV ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl of cells suspension infected with virus was plate micromass cultures (3-4 spots per 35mm culture dish, NUNC #150318) at a density of 2 x 107 cells/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... and then 10 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Life Technologies) was added to the cells and they were incubated for one hour ...
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bioRxiv - Cell Biology 2022Quote: ... DCFH-DA (6-carboxy-2′,7′- dichlorodihydrofluorescein diacetate) was from Invitrogen. DMSO and Evans Blue Dye were purchased from Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were mounted in antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and visualized with IX81 confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: ... and mounted on slides with Prolong Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). Imaging was performed using a Nikon A1 Confocal microscope.
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bioRxiv - Genomics 2021Quote: ... Nuclei were sorted by gating on the DAPI peaks using a BD FACS Aria III (200,000 – 400,000 events) into a small volume of landing buffer (4% BSA in PBS, 2 U/µl Ambion RNase Inhibitor ...
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bioRxiv - Biochemistry 2020Quote: ... and 4 mM TCEP using a 2 mL 7,000 MWCO Zeba spin desalting column (ThermoFisher Scientific, Waltham MA). The desalted protein was incubated with 40 μL of 10 mM AlexaFluor-647 C2 maleimide (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... Masson’s trichrome or 4’,6-diamidino-2-phenylindole (DAPI) mounting media (ProLong® Gold Antifade Mountant, Thermo Scientific). Representative light micrographs were captured with the Olympus VS-110 Virtual Imaging System and fluorescent micrographs with a Zeiss Axioplan2 system.
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bioRxiv - Cancer Biology 2020Quote: Transient transfections were performed by adding 2-3μg of the appropriate vector in conjunction with 4-6μL Lipofectamine transfection reagent (Invitrogen). Cell culture media was changed 12 hours post-transfection and replaced with fresh media ...
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bioRxiv - Neuroscience 2020Quote: ... the tissues were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and mounted with Prolong Gold (Life Technologies). Fluorescence was detected using an epifluorescence microscope (Nikon Eclipse NiE).
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bioRxiv - Biochemistry 2020Quote: ... 2 mL and 4 mL glass vials and ultraclean Silicone/PTFE septa caps (Thermo Fisher, Waltham, MA, USA) were used for biology ...
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bioRxiv - Bioengineering 2020Quote: ... Cells migrating through the porous membrane are identified by staining with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen), counting five representative fields per insert ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed in TNT and stained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, catalog # D3571), washed again ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were mounted on slide using Antifade mounting media with DAPI (4-6-diamidino-2-phenylindole; ThermoFisher). After sealing the coverslips with clear nail polish ...
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bioRxiv - Cell Biology 2021Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 µm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Cell Biology 2021Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 µm, Thermo Fisher Scientific). The column temperature was held at 30°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) in ProLong Diamond Antifade Mountant (Thermo Fisher # P36971). Images were captured using a Nikon Eclipse Ti2 microscope ...
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bioRxiv - Microbiology 2021Quote: ... Cover slips were mounted using Prolong Gold Antifade reagent containing 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies) and incubated overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The cellular nucleus was stained in blue using 4’,6-diamidino-2-phenylindole (DAPI, Fisher Scientific, Illkirch, France). Uninfected DPSCs were used as negative controls.
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bioRxiv - Cell Biology 2021Quote: ... Cells were permeabilized in FACS Buffer (DPBS without calcium and magnesium, 4% FBS, and 2 mM EDTA (Gibco)) with 0.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Nucleus staining reagent 4’,6-diamidini-2-phenylindole (DAPI, #D1306) and Hoechst33342 (#H21492) were purchased from Thermo Fisher Scienfitic.
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted using prolonged diamond anti-fade mounting media containing DAPI (4, 6-diamidino-2-phenylindole; Invitrogen) and imaging was done on Nikon laser scanning confocal microscope Plan Apo VC 100X (oil ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated for 30 min at 37°C with the secondary antibody diluted in PBS/3% BSA/0.05% Tween 20 (see antibodies section) and 4’,6-diamidino-2-phenylindole (DAPI 0.125 mg/ml, Invitrogen) in PBS/3% BSA/0.05% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were mounted on slides with proLong gold antifade reagent with 4’,6-diamidino-2-phenylindole (Invitrogen, USA), which was used to probe for the nucleus.
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bioRxiv - Microbiology 2022Quote: ... the cells were rinsed in PBS and the nucleus stained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher). Coverslips were then mounted and imaged with the Zeiss Laser Scanning 710 confocal microscope images were processed using Zeiss Zen 2011 software.
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bioRxiv - Synthetic Biology 2022Quote: ... 3,4-dihydroxystyrene and 2-methoxy-4-vinylphenol concentrations were quantified using a Dionex UltiMate 3000 HPLC (Thermo Scientific) with a Discovery® HS-F5-3 column (15 cm x 2.1 mm ...
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bioRxiv - Developmental Biology 2019Quote: ... and the rest was immunoprecipitated at 4°C for 2 h using Dynabeads Protein A (Life Technologies, # 10008D)-conjugated anti-m6A antibody (Synaptic systems ...