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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). Expression of circRNAs was calculated relative to the housekeeping genes 18S or ACTB ...
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bioRxiv - Cell Biology 2020Quote: ... Enteroids were passaged every 5 to 7 days by incubating in 0.25% trypsin 0.5 mM phosphate buffered saline-ethylenediaminetetraacetic acid (PBS-EDTA, Gibco) solution for 3 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Whole bone marrow cultures were then cultured in 10 cm non-tissue culture treated dishes for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... bone marrow monocytes were immuno-magnetically isolated and culture for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... Real-time qPCR reactions were performed using the QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Immunology 2020Quote: ... splenic NP366-374 TMEM were assessed with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay kit (ThermoFisher). Lung single cells were stained with surface markers then incubated with caspase 3/7 green detection reagent for 30 minutes at 37°C as described in the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... siRNAs (3μl of 10μM conc.) and RNAiMax (7 μl) were mixed separately with 75 μl Optimem (Thermofisher, 31985062). Knockdown was initiated by mixing both siRNA and RNAiMAX suspensions together ...
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bioRxiv - Cell Biology 2022Quote: WT organoids passage 10 were collected 5-7 days after passaging and digested with TrypLE (Thermo Fisher Scientific) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal neurons were transfected at 7 days in vitro (DIV) using Lipofectamine 2000 (Thermo Fisher, Cat. no. 11668019). Experiments were carried out after 20-24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... with 7 to 14 cycles of PCR using the Phusion High-Fidelity PCR Master Mix (Thermo Fisher Scientific). The ctDNA yield was quantified with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Technical replicates for each experimental sample were analyzed in a ViiA 7 Real-Time PCR system (Applied Biosystems) using the standard curve method ...
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bioRxiv - Developmental Biology 2022Quote: ... together with its IgG2a kappa PE/Cyanine 7-Isotype control (1:500, Thermo Fisher Scientific, 25-4321-81), CD31-APC with human specificity for HUVEC experiments (1:500 ...
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bioRxiv - Cell Biology 2022Quote: COS-7 cells (ATCC CRL-1651) were cultured in DMEM (5.56 mM glucose, glutaMAX supplement, Thermo Fisher 10567022) supplemented with 10% fetal bovine serum (ThermoFisher 10438-034) ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: COS-7 cells were transiently transfected with the relevant DNA constructs using Lipofectamine 2000 (Invitrogen, catalog number 11668). The media was collected ...
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bioRxiv - Genomics 2022Quote: ... Reactions were carried out on a QuantStudio 7 Flex 384-well Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Genetics 2022Quote: ... and expression levels were quantified in the QuantStudio™ 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using 2xSybRGreen Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... and expression levels were quantified in the QuantStudio™ 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions of 7 to 12 representing the translation level were taken for RNA isolation with TRIzol reagent (Ambion). To perform RNA-seq experiment ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized from 7 µl of RNA using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher). TaqMan assays (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... was added to each well and amplification continued in a QuantStudio 7 Flex qPCR machine (Thermo Fisher Scientific) until signal plateaued out of exponential amplification using the following programme ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR assays were conducted using a QuantStudio 7 Pro Real Time PCR system from Thermo Fisher Scientific Inc ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we ran two technical replicate reactions in an QuantStudioTM 7 Flex Real-Time PCR System (Applied Biosystems™). We used 4μL of genomic template ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained cell suspension was passed through a cell strainer and 7-aminoactinomycin D (Molecular probes by Life Technologies) was added for dead cell identification ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained cell suspension was passed through a cell strainer and 7-aminoactinomycin D (Molecular probes by Life Technologies) was added for dead cell identification ...
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bioRxiv - Neuroscience 2023Quote: ... equal amounts of protein were loaded in 7% Bis-Tris gels and run with MOPS buffer (Thermo Scientific) and transferred to membranes (Amersham GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... washed with 1 x PBS (pH 7) and subsequently labelled with 25 μg/ml AF488 C5 Maleimide (Invitrogen) for 10 min in the dark followed by at least two additional washing steps ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR for pri-let-7 and eft-2 were performed with Absolute Blue SYBR Green (Thermo Scientific) on the CFX63 Real Time System Thermocyclers (Biorad ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR (qPCR) reactions were performed with SYBR green (CWBiotech, CW2621) on the QuantStudio 7 Pro (Applied Biosystems) qPCR instrument ...
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bioRxiv - Microbiology 2023Quote: ... and then primers listed in Supplemental Table 2 on a ViiA 7 real-time PCR system (Applied Biosystems) with the following cycling parameters ...
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bioRxiv - Microbiology 2023Quote: ... the cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions for kinetic assays and fluorescence in the live cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed twice and resuspended in FACS buffer containing 7-AAD viability staining solution (Life Technologies). Cell sorting was performed using a BD FACSAria III (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001 ...
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bioRxiv - Bioengineering 2023Quote: ... HUVECs were cultured for 6-7 days prior to detachment with 0.25% trypsin-EDTA (Life Technologies, 25200-056) for use in experiments ...
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bioRxiv - Biophysics 2023Quote: ... potassium phosphate buffer (KP) 0.5 M at pH = 7 (adjusted with pH meter Orion Star A111, Thermo Fisher). BSA and urease stock solutions concentration are measured after their preparation using a UV-vis spectrophotometer (Cary 60 Spectrophotometer ...
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bioRxiv - Neuroscience 2023Quote: ... 7×106 cells were plated in 12ml of transfection media (Opti-MEM I Reduced Serum Medium (Gibco #31985070), 1x GlutaMAX Supplement ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Real-time quantitative RT-PCR (Q-PCR) was performed in a QuantStudio 7/12k Flex System (Applied Biosystems) with the following primers Crbn.F 5’-TGAAATGGAAGTTGAAGACCAAGATAG-3 ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggregation medium (BSA supplemented N2B@7 media) consisted in 500ml 1:1 mix of Neurobasal (Invitrogen 21103-049) and DMEM/F12 (Invitrogen 21331) ...
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bioRxiv - Molecular Biology 2023Quote: ... The qRT–PCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System (Thermo Fisher Scientific). The sequences of primers and probes for Adar1 p150 and GAPDH have been previously reported (16) ...
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bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...
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bioRxiv - Cell Biology 2023Quote: ... MCF-7 cells (HTB-22) and A431 cells (CRL-1555) were all cultured in DMEM (Gibco, 31966-021) supplemented with 10% FCS (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... This solution was incubated for 1h at ~7°C with 0.25 ml of HisPur Cobalt resin (Thermo Scientific). The beads were allowed to settle to the bottom of a protein purification column and washed 5 times with 10 ml of washing buffer comprising 10 mM imidazole and 0.1% Triton X-100 in 2xPBS ...
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bioRxiv - Biophysics 2023Quote: ... The DTT was removed using a 2 ml ZebaSpin MWCO 7 kD desalting column (Thermo Fisher Scientific, USA). The columns were pre-equilibrated by washing them with 1 ml 10 mM TRIS buffer at pH 7.5 for three consecutive times by spinning them at 1000 G for 2 minutes for each washing step ...
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bioRxiv - Cell Biology 2022Quote: Live/Dead™ Cell Imaging Kit and CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) were used for detection of cell apoptosis based on the manufacturer instructions ...
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bioRxiv - Biophysics 2023Quote: ... The protein was then buffer-exchanged into HBS using a 7 kDa Zeba desalting column (Thermo Fisher, 89882) and stored at -80 ºC until use.
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bioRxiv - Genomics 2022Quote: ... HPC-7 cells were maintained at density of 2-10 x105/ml in Iscove’s modified Dulbecco’s medium (Invitrogen) supplemented with 50 ng/ml of mouse stem cell factor (Gemini Bio-Products) ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence data for SYPRO Orange signal was collected with a ViiA 7 Real-Time PCR System (Applied Biosystems) and QuantStudio Real-Time PCR Software v1.2 using 470 ± 15 nm excitation and 586 ± 10 nm emission filter ...
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bioRxiv - Biochemistry 2023Quote: ... for 40 min before neutralizing to approximately pH 7 with 2 M Tris (Thermo Fisher Scientific, Waltham, MA) in water ...
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bioRxiv - Immunology 2023Quote: ... 10% H2/N2 (BOC) for 3 days on blood agar plates containing 7% laked horse blood (Thermo Scientific) and the Campylobacter selective supplement “Skirrow” containing the antibiotics trimethoprim ...
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bioRxiv - Molecular Biology 2023Quote: ... transfections were performed by adding the following to each well: 7 µL of pre-warmed OptiMEM media (Invitrogen), 500 ng of 5′ -capped and 3′ -polyadenylated Nluc reporter mRNA ...