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Citations for Thermo Fisher :
1901 - 1950 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Nuclei were counterstained using 5μg/ml of DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride; D1306, Invitrogen) added during secondary antibody incubation.
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bioRxiv - Cell Biology 2020Quote: ... spleens from C57Bl/6 mice were smashed through a 70 µm cell strainer (Thermo Fisher Scientific). After red blood cell lysis (1:10 dilution for 3 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... : DAPI Buffer (106mM MgCl2, 50 µg/mL 4’, 6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat# D1306), 5mM Ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instruction and carried out on Quant Studio 6 Flex System (Applied Biosystems). Relative microRNA expression was calculated by the 2−ΔΔCT method and RnU6b was used as the internal control for normalization.
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bioRxiv - Cell Biology 2022Quote: ... YPD plates containing 0.33 M acetate pH 6 were prepared by adding 3M sodium acetate (Ambion) to 1L sterilized YPD ...
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bioRxiv - Developmental Biology 2022Quote: ... Each well was incubated with pre-warmed enzymatic mix of 6 mg/ml Dispase (Gibco, 17105041) and 1 mg/ml Liberase TH Research Grade (Roche ...
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bioRxiv - Developmental Biology 2022Quote: A total of 500K cells were seeded per well of 6-well plate (Thermo Scientific, # 1483210) to reach confluent monolayer ...
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bioRxiv - Bioengineering 2022Quote: ... levels of mIL-6 and mTNF-α were estimated using their respective ELISA kits (ThermoFisher Scientific).
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bioRxiv - Physiology 2022Quote: ... CellTracker Green CMFDA Dye (C2925) and DAPI (4’,6-diamidino-2-phenylindole) (D1306) were from Invitrogen. EZ-Link Sulfo-NHS-LC-LC-Biotin (#21135 ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was run on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific) with PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... either alone or with anti-IL-1β or anti-IL-6 neutralizing antibodies (Thermofisher, Waltham, MA), and ensuing protein lysates blotted for phosphorylated STAT-3 (pSTAT3) ...
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bioRxiv - Cell Biology 2022Quote: ... 3-6 dpf larval zebrafish were anesthetized using 0.2 mg/mL Tricaine-S (Fisher Scientific, NC0872873) solution ...
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bioRxiv - Cancer Biology 2020Quote: ... and Type 6 primary probes targeting MYCN were designed and synthesized by Affymetrix (Supplementary Table 2). Hybridization was performed according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... on 6-well plates (Thermo Fischer: 140675) coated with Geltrex basement membrane matrix (Thermo Fisher, A1413302). Cells were passaged upon reaching 60-70% confluency ...
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bioRxiv - Developmental Biology 2020Quote: ... Other media components included Insulin-Transferrin-Selenium-Ethanolamine (ITS-X; Thermo Fisher Scientific; days 6-10), retinoic acid (RA ...
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bioRxiv - Molecular Biology 2020Quote: ... Cloned vectors (1.5 μg each per 6 well plate well) were transfected using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions into U2OS PER2::LUC cells ...
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bioRxiv - Immunology 2020Quote: ... T cells from C57BL/6 mice were further labeled with CellTrace™ Violet (Invitrogen cat. C34557). BMDCs were washed three times and co-cultured with naïve CD4+ T cells at a ratio of 1:10 for 72 h ...
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bioRxiv - Neuroscience 2021Quote: Primary mouse cortical neurons (MCNs) from C57BL/6 embryonic day 17 were purchased from Thermo Fisher Scientific (cat ...
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bioRxiv - Developmental Biology 2021Quote: ... Soluble fractions were run by SDS-PAGE on 6% tris-glycine gels (Life Technologies, cat# XP00065BOX). Electrophoresis were run in Tris-glycine-SDS buffer (Wisent ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Nuclei were then stained with 0.01% w/v 4’,6-diamidino-2-phenylindole dilactate (DAPI, Invitrogen) for 5 min and rinsed with ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... and the retained spheroids were collected by washing the strainer with Essential 6 medium (Thermo Fisher). Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies ...
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bioRxiv - Genomics 2022Quote: ... qPCR was performed in duplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Viral load was calculated as RNA copies per microgram of total RNA ...
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bioRxiv - Genomics 2022Quote: ... human preadipocytes in a 6-well plate were transfected with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
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bioRxiv - Cell Biology 2022Quote: Diaphragm intracellular ROS was measured using 6-carboxy-20,70-dichlorodihydrofluorescein diacetate (DCFH-DA) (Invitrogen, Carlsbad, CA) as previously described14 Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Non-viable cells were excluded by staining with 4’,6-diamidino-2-phenylindole (Thermo Fisher, D1306). Flow-cytometric data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... qPCR was performed using the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Waltham, MA) where RQ values were normalized against gene expression on day 0 for each replicate time course and β-actin was used as a reference gene ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted beads were then treated with 6 M guanidine hydrochloride (GdnHCl; [Thermo Fisher Scientific; 24115]) and incubated for 2 hrs at room temperature with mixing ...
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bioRxiv - Neuroscience 2020Quote: ... the skin was sutured after the viral injection using 6-0 suture silk (Fisher Scientific NC9134710). To reverse the anesthesia induced by the Fentanyl-Midazolam-Dexmedetomidine mixture ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were plated in 6 well plates for RNA isolation experiments according the guidelines from ThermoFisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... and each explant was pre-treated for 6 h with fresh modified Basal Medium Eagle (Invitrogen) containing TUDCA (750µg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were counterstained with 300nM 4’,6-diamidino-2-phenylindole dilactate (DAPI) (D1306, ThermoFisher Scientific, UK) for imaging assessment (Zeiss Axio observer Z1 microscope) ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were stained with 300 nM 4’,6-diamidino-2-phenylindole nucleic acid stain (DAPI; Invitrogen) in PBS for 5 min followed by washing three times in PBS at room temperature ...
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bioRxiv - Immunology 2020Quote: ... by the Applied Biosystems™ QuantStudio™ 6 standard Real-Time PCR System (Thermo Fisher Scientific). The housekeeping genes β-actin was routinely used as internal controls ...
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bioRxiv - Cancer Biology 2019Quote: ... then treated with a Protein Tyrosine Kinase 6 (PTK6) inhibitor (dasatinib, catalogue # NC0713371, Thermo Fisher Scientific) or PAK4 inhibitor (vandetanib ...
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bioRxiv - Physiology 2019Quote: ... The qPCR assays were run on a QuantStudio 6 real-time PCR system (Thermo Fisher Scientific) and each sample was run in triplicate with 2 µL of the diluted cDNA and 8 µL of master mix containing 1x qPCR MasterMix Plus Low ROX (Eurogentec ...
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bioRxiv - Cell Biology 2019Quote: ... DNA is stained with 1 mg/ul 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher, Carlsbad, CA) 1/10,000 in PBS for 5 minutes and Vectashield mounting medium is applied (Vector Labs ...
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bioRxiv - Cell Biology 2019Quote: ... DNA is stained with 1 mg/ul 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher, Carlsbad, CA) 1/10,000 in PBS for 5 minutes and Vectashield mounting medium is applied (Vector Labs ...
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bioRxiv - Bioengineering 2019Quote: ... Sections were also counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1:1000; Invitrogen, cat. #D1306). Immunocytochemical images were acquired with a confocal microscope (Olympus FV1000 Laser Scanning Confocal ...
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Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetesbioRxiv - Cell Biology 2019Quote: Pancreas tissue was harvested from wildtype C57BL/6 mice and fixed in 4% paraformaldehyde (Fisher Scientific), cryo-preserved using 30% sucrose ...
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bioRxiv - Physiology 2019Quote: ... the digested peptides were labelled with a TMT 6-plex mass tagging kit (Thermo Fisher Scientific) according to the manufacturer’s instruction and subsequently pooled ...
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bioRxiv - Genomics 2020Quote: ... Media was changed ∼6 hours post transfection to DMEM collection media (10% FBS (Thermo Fisher Scientific), 1% Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative real-time polymerase chain reactions (qRT-PCR) were performed in QuantStudio 6 Flex (Applied Biosystems) equipment using a final volume of 10 μL per well with 2x Power SYBR Green RT-PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... shTMEFF2-6 and shTMEFF2-9 sequences were generated using the siRNA Wizard online tool by Invitrogen: https://www.invivogen.com/sirnawizard/) ...
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bioRxiv - Cancer Biology 2019Quote: ... and H146 cells was measured 6 days after inhibitor treatment using Cyquant Direct Proliferation Assay (Invitrogen) and an Optima Fluostar plate reader (BMG LabTech) ...
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bioRxiv - Cell Biology 2019Quote: ... also confirmed by 4′,6-diamidino-2-phenylindole fluorescent dye (DAPI, 1:1000, Molecular Probes, USA) staining.
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bioRxiv - Physiology 2020Quote: ... on a QuantStudio 6 Real Time PCR machine (Applied Bio Systems, Life Technologies, Carlsbad, CA, USA). Relative gene expression was normalized to the reference gene ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was gel-size selected by running samples on a precast 6% TBE-urea gel (Invitrogen) at 180V for 40 min and isolating the region between 75 and 200 nt ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse TNF-α and IL-6 ELISA Ready-SET-Go! kits (Affymetrix, San Diego, CA, USA) were used for the detection of tumor necrosis factor α (TNF-α ...
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bioRxiv - Neuroscience 2021Quote: ... retinal cultures were incubated at P12 for 6 h with the CMAC substrate (Invitrogen, Carlsbad, CA), at a concentration of 50 μM in the culture medium ...
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bioRxiv - Genomics 2019Quote: ... The pancreatic acinar cell line 266-6 (ATCC CRL-2151) was grown in DMEM (Thermo Fisher) with 10 % fetal calf serum ...