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Citations for Thermo Fisher :
1851 - 1900 of 6996 citations for sCD4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 20ng/ml human bFGF (PHG6015, ThermoFisher Scientific). SB 431542 (1614 ...
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bioRxiv - Biophysics 2024Quote: ... 20 ng/mL human EGF (PHG0311, Gibco), 0.5 µg/mL hydrocortisone (H0888–1G ...
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bioRxiv - Cell Biology 2024Quote: ... human Kif18A siRNA (Ambion, #Cat 4390825, ID:s37882), human Kif22/Kid siRNA (Ambion ...
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bioRxiv - Physiology 2024Quote: Human aortic smooth muscle cells (HASMCs, ThermoFisher) were cultured in smooth muscle cell media (ScienCell ...
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bioRxiv - Cancer Biology 2024Quote: ... 20ng/mL recombinant human EGF (Life Technologies), 20ng/mL recombinant human FGF-basic (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10μg/ml human recombinant insulin (Gibco) at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20ng/mL recombinant human EGF (Life Technologies), 20ng/mL recombinant human FGF-basic (Fisher Scientific)] ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary human hepatocytes (Gibco/Thermo Fisher Scientific) were thawed into a 24-well Collagen I coated plate (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary human hepatocytes (Gibco/Thermo Fisher Scientific) were thawed into a 24-well Collagen I coated plate (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and 5 mg/mL human insulin (ThermoFisher) freshly supplemented with 100 ng/mL IL-34 ...
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bioRxiv - Immunology 2024Quote: ... biotin anti-human IgA Fab (Invitrogen A24462) was added at a dilution of 1:100,000 for 2 hours at 4 C to detect human IgA ...
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bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
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bioRxiv - Molecular Biology 2024Quote: Signaling via the intracellular Ca++ pathway was assessed in stably transfected HEK- HEK293/hPTH1R/glosensor (GP-2.3) cells using the calcium-sensitive fluorophore Fura2-AM (Invitrogen, Life Tech. Grand Island, NY, USA) [73 ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR assays were performed using TaqMan Gene Expression Master Mix and the probes human NEMO/IKBKG (Hs00415849_m1) and human GAPDH (Hs03929097_g1) (ThermoFisher Scientific). Amplifications were run in a Bio-Rad CFX96 real-time PCR system ...
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bioRxiv - Biochemistry 2020Quote: ... The second incubation were performed with secondary antibody (Anti-Human IgG-Cy3, Jackson ImmunoReserarch; Anti-Human IgM-Cy5, Invitrogen) diluted in ratio 1:1000 in incubation buffer with 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: Human monocytic U937 cells (ATCC) and human embryonic kidney 293T cells (ATCC) were cultured in RPMI 1640 medium (Gibco), supplemented with 5-10% fetal calf serum (BioWest) ...
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bioRxiv - Cancer Biology 2021Quote: Human HCC cell lines (Huh7, HepG2) and Human HeLa were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco-BRL) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... Human AFP secreted into the sera of animals were determined by the Human AFP Elisa Quantification Kit (Invitrogen, EHAFP) following manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... In the analysis of the Woroniecka Human Diabetic Kidney Disease dataset (2011, Affymetrix Human Genome U133A 2.0 Array platform) 33 and the European renal cDNA bank (ERCB ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK293T) and human neuroblastoma (SH-SY5Y) cells from ATCC were cultured in high glucose DMEM (Invitrogen) and DMEM-F12 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting human UBE3A or human MASTL (Integrated DNA Technologies) was transfected into cells using Lipofectamine RNAi MAX (Invitrogen). A non-targeting control siRNA was used as a control ...
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bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
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bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
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bioRxiv - Molecular Biology 2023Quote: ... Hep-3B human hepatocellular carcinoma cells and MCF7 human mammary carcinoma cells were cultured in Eagle’s Minimum Essential Medium (EMEM) (Gibco) supplemented with 10% heat-inactivated and charcoal stripped fetal calf serum and antibiotics ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human melanocytes were grown in Melanocyte Growth Media 254 supplemented with Human Melanocyte Growth Supplement-2 (Life Technologies), calcium chloride (0.3 μM) ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
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bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells (HUVECs) and human coronary artery smooth muscle cells (HCASMCs) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... human breast cancer MCF7 cells and human bone osteosarcoma U2OS cells were in cultured in DMEM medium (Life Technologies) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The ORF of red fluorescent protein gene (RFP)-fused Antp was inserted into a pIZ/V5-His vector (Invitrogen) driven by the OpIE2 promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged Gβγ was expressed in Sf9 cells for 2 days following baculovirus infection (pFastBacDual, Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: His-tagged P-Rex2 was expressed in Sf9 cells for 2 days following baculovirus infection (Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems] ...
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bioRxiv - Biochemistry 2020Quote: ... Binding of the full-length S protein was detected by mouse anti-His IgG (Invitrogen MA121315, 2 μg/ml) and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001 ...
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bioRxiv - Biochemistry 2020Quote: ... Purified PCR products were digested with XhoI and NheI and inserted into the pcDNA6/V5-His expression vector (Invitrogen) generating plasmid pMBA40 for POMGNT1 complementation ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA libraries were pooled for emulsion PCR using an Ion PI Hi-Q Chef Kit (Thermo Fisher Scientific). The enriched samples were loaded onto an Ion PI chip v3 with Ion Chef ...
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bioRxiv - Genomics 2021Quote: ... Sequencing was performed on the Ion Torrent PGM with the Ion PGM Hi-Q View Sequencing kit (Life Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the RNA-Seq templates were prepared using the Ion PGM Hi-Q View OT2 Kit (Thermo Fisher Scientific Inc.) on an Ion OneTouch 2 system ...
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bioRxiv - Microbiology 2020Quote: 293F cells were transfected by plasmids pOptiVec/V5-His WEAU gp120-trimer using 293fectin according to the manufacturer’s (Invitrogen) instructions ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.
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bioRxiv - Immunology 2022Quote: ... and C-terminal foldon trimerization motif followed by hexa-His tag) were used to transiently transfect Expi293F cells (Gibco). Four days after transfection ...
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bioRxiv - Immunology 2022Quote: ... two alpacas were five times immunized with 200 µg His-NLRP1PYD using Imject™ Alum Adjuvant (Thermo Fisher Scientific) according to locally authorized protocols ...
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bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat. #MA1-21315-1MG, ThermoFisher Scientific) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... fused with an N-terminal 6×His-tag and cloned into expression vector pPIC9 (Thermo Fisher Scientific, California, USA). Correctness of the resulting constructs was confirmed by DNA sequencing prior to introduction into Pichia pastoris strain GS115 (Thermo Fisher Scientific ...