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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Recombinant Mouse Fgfr1 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... bacteria were tagged by Gfp or stained by cell membrane stain FM4-64 (10 µM final concentration, Molecular Probes) for 2 mins in the dark (without flow) ...
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bioRxiv - Cell Biology 2021Quote: ... Stable cell lines expressing BioID tagged genes of interest were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5/FRT/TO construct and Flipase expressing pOG44 plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids for expressing tagged proteins in S2 cells were generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF) ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Cells at 40% confluence were transfected with 1 ug of GFP-tagged LRRK2 plasmid using Lipofectamine 2000 (Invitrogen, 11668019) as described for 12-15 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
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bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-tagged proteins were applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified A-C/EBP (30 - 40 μg) was tagged with Alexa Fluor 488 according to manufacturer’s protocol (Thermo fisher). Time lag expression of A-C/EBP protein was observed using EGFP tagged A-C/EBP plasmid (pCMV-A-C/EBP-EGFP ...
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bioRxiv - Immunology 2019Quote: ... The labeled cells were counter-stained with a fluorophore-tagged secondary goat anti-rabbit IgG (H+L) antibody (Invitrogen), before acquisition on a LSR Fortessa (BD Biosciences) ...
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bioRxiv - Molecular Biology 2019Quote: ... SmBChE1 and SmAChE3 (fSmChEs) were EcoRI/XbaI cloned into the C-terminal 6-His-tagged pPICZαA expression vector (Invitrogen) to facilitate secretory expression ...
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bioRxiv - Biophysics 2020Quote: ... The C-terminally SBP-tagged DISP1-A or DISP1-A-NNN was captured using Streptavidin-agarose affinity resin (ThermoFisher). The eluted protein was concentrated by an Amicon Ultra filter (100 kDa cutoff ...
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bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were subsequently washed with TSB and incubated with WGA tagged with Alexa Fluor 488 (WGA-488, Invitrogen) at a final concentration of 2 μg/ml for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Transgenic S2 cells carrying the SIN3 187 transgene tagged with an HA epitope were grown in medium containing 0.1 mg/ml penicillin and streptomycin (Gibco). For treatment with CuSO4 ...
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bioRxiv - Plant Biology 2023Quote: ... Epitope-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... mYFP tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with uLimch1 and mLimch1 epitope tagged expression plasmids using Lipofectamine 3000 following the manufacturer’s protocol (catalog number L3000015, Invitrogen). mLimch1 and uLimch1 epitope tagged expression plasmids were commercially synthesized by GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... the C-terminal HA-tagged Cas12f/sgRNA-encoding plasmids were transfected into HEK293T cells by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed twice with PBS and stained with AlexaFluor 488-tagged donkey-anti-goat (Molecular Probes, 1:100), Cy3-tagged donkey-anti-chicken (Jackson Immuno ...
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bioRxiv - Biophysics 2023Quote: ... and the 3C protease was removed using Dynabeads magnetic beads designed for His-tagged protein isolation (Thermo Fisher, 10103D). The supernatant from the magnetic bead pulldown was then concentrated using Amicon 10 kDa centrifugal filter units (MilliporeSigma) ...
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bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
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bioRxiv - Synthetic Biology 2023Quote: Tetra-cysteine (TC) tagged proteins (CCPGCC) were site-specifically labelled using the TC-ReAsH II TC detection dye (Invitrogen). TC-HERD-2.2–TnaA and TC-HERD-2.2–FMO (100 μM ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged TpPyShell together with TEV protease was dialyzed overnight at 4°C in SnakeSkin dialysis tube (Thermo Scientific) against dialysis buffer (50 mM Tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were gently mixed with a 2x solution such that the final concentrations were 0.5mg/mL Alexa Fluor 647-tagged 10,000 MW dextran (Invitrogen #D22914), 200nM caged fMLP (NEP) ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were seeded in 6-well plates and transfected with 0.25μg LgBiT-miniG (miniGs or miniGsq45) or LgBiT-β-arrestin240 and 0.25μg SmBiT-tagged receptor plasmids using 3µL Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... or Flag-tagged proteins were generated by inserting PCR-amplified fragments into the pcDNA3.1(+) mammalian expression vector (V79020, Invitrogen). Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1 ...
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bioRxiv - Developmental Biology 2019Quote: ... Stromal cells were suspended in DMEM: F12 Nutrient Mixture (Hyclone) containing 10% heat-inactivated FCS (Gibco), 50 units/mL penicillin ...
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bioRxiv - Immunology 2019Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Biochemistry 2019Quote: ... The cells were then incubated using FITC-labelled goat-anti-human IgG-FC antibody (ThermoFisher Scientific) (200 ng/ul ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
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bioRxiv - Immunology 2021Quote: ... Digestion was stopped with RP-HE (RPMI 1640, Thermo Fisher; 10% FCS (Corning, #35-079-CV), 10 mM EDTA (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The plates were read with a Multiskan FC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA) at 570 nm ...
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bioRxiv - Biochemistry 2020Quote: ... An in-frame fusion between ACE2 and Fc was constructed in pcDNA3 vector (Invitrogen, Carlsbad, CA). Site-directed mutagenesis by PCR was performed to create the panel of ACE2-Fc mutants ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cell density was measured as an optical density (OD, 595 nm; Multiscan FC Thermo Scientific) and colony forming units per ml (CFU/ml ...
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bioRxiv - Microbiology 2020Quote: ... RBD-Fc was biotinylated with EZ‐link sulfo‐NHS‐LC‐biotin (Thermo Fisher Scientific, Waltham, MA) (RBD-Fc-Bio) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with final 10 % (v/v) fetal calf serum (FCS, Thermo Fisher Scientific, Waltham, MA, USA), 1x non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest, Nuaillé, France) and antibiotics (Gibco, Waltham, MA, USA). The human NSCLC cell line NCI-H23 (H23 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco). Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells ...
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bioRxiv - Biophysics 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... S2R+ Cells (2 × 105) were seeded in Schneider medium plus 10% FCS (Gibco 21720024, Sigma F9665) in a 24-well plate ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 % fetal calf serum (FCS) (Biochrom, Ltd., Berlin, Germany) and 1 % penicillin-streptomycin (Invitrogen). The human colorectal adenocarcinoma cell line Caco-2 was provided by Jindrich Cinatl Jr ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were diluted to 5 x 105 cells/mL in RPMI + 10% FCS (Gibco) and treated with 100 nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were pre-incubated with Fc-Block (eBioscience, Thermo Fisher Scientific, Les Ulis, France) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and absorbance were subsequently measured at 450 nm using a Multiskan™ FC photometer (Thermo Fisher).