Labshake search
Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Recombinant Mouse Acvr2a His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: Cloning and expression of His-mVenus-SUF4 was based on the Bac-to-Bac Baculovirus Expression system (ThermoFisher). The control pFastBac-mVenus and pFastBac-mVenus-SUF4 isoform 1 plasmids were obtained from Dr ...
-
bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were co-stained with recombinant Cholera toxin subunit B conjugated to Fluor488 (Invitrogen C34775) at 1 μg/mL for less than 1 minute prior to fixing and staining for NINJ1 as described above ...
-
bioRxiv - Physiology 2019Quote: Recombinant adenoviruses were generated using the pAd/CMV/V5-DEST Gateway recombination system (Life Technologies) after cloning the full-length cDNA into the pENTR vector ...
-
bioRxiv - Biochemistry 2019Quote: ... The resulting antiserum was affinity purified using recombinant CTLMA2 immobilized on Aminolink resin (Thermo Fisher). Western blotting was performed with affinity purified anti-CTLMA2 ...
-
bioRxiv - Immunology 2019Quote: ... The protein was expressed in Sf9 insect cells using a recombinant baculovirus system (Life Technologies). The protein was purified on a cobalt resin column and further purified by gel filtration to ensure the removal of monomeric and aggregated protein.
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant BACs were isolated from the bacterial hosts and used to transfect Sf9 cells (Invitrogen).
-
bioRxiv - Biochemistry 2021Quote: ... The recombinant IgG (XBA14 and XBA18) was produced in FreeStyle 293 cells (Thermo Fisher Scientific), and purified by Protein A-Sepharose (Cytiva ...
-
bioRxiv - Bioengineering 2019Quote: ... and genomic DNA was digested using Optizyme™ recombinant DNase-I digestion kit (Thermo Fisher) per manufacturers’ instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... The recombinant PfAdoMetDC protein was purified using the Strep-Tactin Sepharose column (Invitrogen, California, USA) as described previously (29 ...
-
bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 10ng/ml recombinant human basic Fibroblast Growth Factor (bFGF) and 1x B27 supplement (Life Technologies). Cells were then counted and passed through a 25G needle to obtain a single cell suspension ...
-
bioRxiv - Cancer Biology 2021Quote: ... The expression of recombinant IL-18 was induced by 0.5 mM IPTG (Thermo-Fisher Scientific) for 8 h at 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with recombinant EGF and bovine pituitary extract according to the manufacturer’s instructions (Thermo Fisher), as well as 100 μg/ml Primocin (Invivogen) ...
-
bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
-
bioRxiv - Microbiology 2020Quote: ... BMDCs were differentiated with recombinant murine granulocyte-macrophage colony-stimulating factor (20 ng/ml; Invitrogen), while BMDMs with macrophage colony-stimulating factor (10ng/ml ...
-
bioRxiv - Immunology 2021Quote: ... Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) and the purity was assessed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE).
-
bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant human TNF-α (PHC3011) and antibody against OAS3 (PA5-31090) was from Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... and 6xHis tags Recombinant baculoviruses were generated using the Bac-to-Bac system (Thermo Fisher). High Five cells (Thermo Fisher B85502 ...
-
bioRxiv - Genetics 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
-
bioRxiv - Microbiology 2019Quote: ... Samples were shifted to RT and treated with 1 µl RNaseOUT Recombinant Ribonuclease Inhibitor (ThermoFisher) and 0.5 µl Thermo Scientific Maxima H Minus Reverse Transcriptase ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
-
bioRxiv - Plant Biology 2021Quote: ... Gateway recombinant cloning was performed to subclone the aforementioned constructs into pDEST™24 (Invitrogen) using a LR cloning reaction (Invitrogen) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The recombinant plasmids were isolated by using Gene JET plasmid miniprep kit (Thermo Scientific, USA). The recombinant plasmids of SCD and SREBP1 genes were transfected into the CEH by using the Gene Pulser XcellTM Electroporation system (Biorad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Recombinant baculoviruses expressing A2AAR WT or mutants were prepared using Bac-to-Bac system (Invitrogen). Spodoptera frugiperda 9 (Sf9 ...
-
bioRxiv - Cell Biology 2021Quote: Binding of recombinant proteins to pre-spotted PIP and membrane lipid strips (Thermo Fisher; P23751) was performed according to (Dowler et al. ...
-
bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 ng Cholera Toxin subunit B (CT-B) (Recombinant Alexa Fluor 488 conjugate, Life Technologies) was injected into the hindbrain ventricle81 ...
-
bioRxiv - Cell Biology 2020Quote: ... Recombinant lentivirus was produced by transient transfection of 293T cells using Virapower packaging mix (Invitrogen). Cells were plated into 2 well LabTek chambered coverglass (Nunc ...
-
bioRxiv - Cell Biology 2019Quote: ... Transposition and the generation of recombinant baculovirus were performed following manufacturer’s protocols (Thermo Fisher Scientific). For protein production ...
-
bioRxiv - Immunology 2020Quote: ... 100uL of recombinant protein at 0.5 ug/ml was coated on Maxisorp™ plates (Nunc) and incubated overnight at 4°C ...
-
bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant CP1Ag was expressed and initially purified via a nickel-affinity column (HisPur Thermo Fisher) as previously described [64] ...
-
bioRxiv - Bioengineering 2022Quote: ... the medium was supplemented with Human Interferon-gamma Recombinant Protein (IFN-γ) (ThermoFisher, Cat. #. PHC4031) at a concentration of 50 ng/mL ...
-
bioRxiv - Cell Biology 2022Quote: ... the recombinant ACE2-Fc protein which was obtained from ExpiCHO™ expression system (Thermo Fisher) was 5-fold serially diluted in DMEM medium supplementing 10% FBS (Gibco) ...
-
bioRxiv - Cell Biology 2022Quote: ... recombinant hACE2-Fc protein was biotinylated using the EZ-Link sulfo-nhs-biotin kit (ThermoFisher) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Integrin-β2 recombinant protein complexes were immobilized on Maxisorp Immuno plates (ThermoFisher, 12-565-135) and used for positive binding selections with library phage pools that were first exposed to neutravidin coated wells to deplete nonspecific binders ...
-
bioRxiv - Cancer Biology 2022Quote: ... we delivered recombinant Streptococcus pyogenes Cas9 protein (TrueCut™ Cas9 Protein v2; Thermo Fisher Scientific) together with the crRNA and tracrRNA into in vitro fertilized eggs of C57BL/6J mice according to the institutional procedure of Kyoto university (Ishibashi et al. ...
-
bioRxiv - Physiology 2023Quote: Balb/c mice were injected subcutaneously (s.c.) with 40IU of EPO (Recombinant HuEpo-alpha, Gibco) for four consecutive days ...
-
bioRxiv - Molecular Biology 2022Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
-
bioRxiv - Pathology 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
-
bioRxiv - Immunology 2024Quote: ... recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
-
bioRxiv - Neuroscience 2023Quote: ... Purified recombinant human antibodies (rHumAbs) were desalted (Zeba™ Spin Desalting Columns, Thermo Fisher 89891) and concentrated using a Christ Rotational Vacuum Concentrator System ...
-
bioRxiv - Physiology 2024Quote: ... according to the manufacture protocol and treated with recombinant Rnase-free DNAse I (Thermo Scientific). RNA concentration and purity were determined with NanoDrop 2000 Spectrophotometer (ND-2000 ...
-
bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2024Quote: ... The Taq DNA Polymerase recombinant PCR kit (Cat no. 10342-020, Thermo Fisher Scientific, USA) was used following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... and plated in the fibroblast medium onto truncated recombinant human vitronectin (VTN-N, Gibco, A14700)-coated plates ...
-
bioRxiv - Developmental Biology 2024Quote: Recombinant FGF10 protein was labeled using an Alexa Fluor 488 Microscale Protein Labeling Kit (Invitrogen). To reduce the loss of physiological activity of FGF10 ...