Labshake search
Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Rat Golgi phosphoprotein 3 GOLPH3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
-
bioRxiv - Microbiology 2022Quote: ... We extracted the genomic DNA of ELISA-positive cattle using either the magLEAD 12gC or a MagMAX™ CORE Nucleic Acid Purification Kit (Thermo Fisher Scientific Inc., Waltham, USA) with an automated nucleic acid extraction system (KingFisher Duo Prime ...
-
bioRxiv - Neuroscience 2023Quote: TNFα and IL-6 released in the microglia conditioned medium were quantified using mouse TNFα and IL-6 ELISA kits according to manufacturer’s instructions (Invitrogen, 88-7324 and 88-7064-22, respectively) and a SpectraMax i3x spectrophotometer (Molecular Devices) ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from dissected ipsilateral and contralateral vMB from AAV-TYR- injected rats using mirVana PARIS RNA and Native Protein Purification Kit (Thermo Fisher Scientific # AM1556). RNA concentration was determined using a NanoDrop ND-1000 Spectrophotometer ...
-
bioRxiv - Microbiology 2023Quote: ... haemoglobin RNA was removed from 1 µg of RNA extracted from blood samples using the GLOBINclear-Mouse/Rat Globin mRNA Removal Kit (Thermo Fisher Scientific; AM1981) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... and mACP-HA2 was visualized with the rat anti-HA 3F10 primary antibody and FITC-conjugated donkey anti-rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on an EVOS M5000 imaging system or Zeiss 880 laser-scanning confocal microscope fitted with an Airyscan detector ...
-
bioRxiv - Microbiology 2023Quote: ... and the endogenously C-terminal HA-tagged cytochromes were visualized with a Roche Rat anti-HA monoclonal 3F10 primary antibody and FITC-conjugated Donkey anti-Rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on DIC/brightfield ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-rat DC217 (1:200, Axon Neuroscience R&D SE, Bratislava, Slovakia) and polyclonal anti-rat pThr212 (1:1000, Invitrogen Life Technologies, Carlsbad, CA). For total tau ...
-
bioRxiv - Cell Biology 2020Quote: The 5’ and 3’ ends of lncRAP2 was determined using the FirstChoice RLM-Race Kit from Ambion following the manufacturers instructions ...
-
bioRxiv - Microbiology 2019Quote: ... Caspase-like activity was measure with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen), a nucleic acid-binding dye that harbors the caspase-3/7 cleavage sequence ...
-
bioRxiv - Developmental Biology 2021Quote: ... 150 ng of each sample was and processed with the Affymetrix 3’ IVT Express Kit (ThermoFisher Scientific).
-
bioRxiv - Genomics 2021Quote: ... and the isolated RNA quantified using a Qubit 3 with the Qubit RNA HS kit (Invitrogen #Q32852). Stranded ...
-
bioRxiv - Cell Biology 2021Quote: ... RNA (3 μg) was converted to cDNA using a SuperScript II cDNA synthesis kit (Thermo Fisher Scientific). cDNA (62.5 ng ...
-
bioRxiv - Plant Biology 2020Quote: ... The small RNA was 3’ poly(A) tailed with Ambion® Poly(A) Tailing Kit (Life Technologies) and 5’ de-capped by using tobacco acid pyrophosphatase (TAP) ...
-
bioRxiv - Plant Biology 2021Quote: ... chip hybridization and scanning were performed according to the manufacturer’s instructions for 3’ IVT PLUS Kit (Affymetrix). Data normalization between chips was conducted using RMA (Robust Multichip Average ...
-
bioRxiv - Cancer Biology 2022Quote: ... the RNAs were labeled with desthiobiotinylate using a Pierce RNA 3’ end desthiobiotinylation kit (20163, Thermo Scientific). An RNA–protein pull-down assay was performed using a Pierce Magnetic RNA–protein pull-down kit (20164 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... 790 ng of the products were biotinylated with Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher Scientific). After ligation of 15 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... The equimolar mRNAs were labeled using biotin with an RNA 3’ End Desthiobiotinylation Kit (Thermo scientific, USA). The labeled mRNAs were then captured using streptavidin magnetic beads (Thermo scientific ...
-
bioRxiv - Cell Biology 2021Quote: zebrafish roraa 3′UTR was amplified from zebrafish genomic DNA with SuperScript III RT-kit (Invitrogen #18080044) and cloned into psiCHECK2 (Promega ...
-
bioRxiv - Neuroscience 2019Quote: ... unc-7 rescue plasmids were made using the Multisite Gateway® 3-Fragment Vector Construction Kit (Invitrogen). A 1078bp mec-4 promoter fragment (as previously used ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The dsDNA quantity was measured using dsDNA HS Assay Kit for fluorometer Qubit 3 (Life Technologies, USA). ddRAD-library was constructed following the quaddRAD protocol (Franchini ...
-
bioRxiv - Microbiology 2022Quote: ... and the resulting 160-bp amplicon biotin-labeled using the Biotin 3’ End DNA Labeling kit (ThermoFisher). EMSA reactions were prepared in 20 µl reaction mixtures in binding buffer (10 mM Tris ...
-
bioRxiv - Molecular Biology 2022Quote: ... peroxidase activity was developed either using AEC (3-amino- 9ethylcarbazole) substrate kit (Life Technologies, Carlsbad, CA, USA) for the cryosections or DAB (3,3’- Diaminobenzidine ...
-
bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...
-
bioRxiv - Developmental Biology 2023Quote: ... sgRNA (5′-TGTGAATCGTAGGAGCGCTG-3′) was synthesized via in vitro GeneArt™ Precision gRNA Synthesis Kit (ThermoFisher, A29377) following manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... and AccuCheck ERF Reference Particles Kit (3 μm, Catalog No. A55950, lot #081220207, #081220203, #081220208, Thermo Fisher) which were prepared according to the manufacturer’s instructions.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) + Tris Buffer HCl pH 7,4 (1:1 ...
-
bioRxiv - Neuroscience 2023Quote: Dead and apoptotic cells were detected using the CellEvent Caspase-3/7 Kit (#C10423, Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2023Quote: ... the C4-2B or PC-3 cells were labeled by CellTrace™ Violet Cell Proliferation Kit (ThermoFisher) according to the manufacturer’s manual ...
-
bioRxiv - Biophysics 2024Quote: ... The 3 mAbs were specifically conjugated to HRP using EZ-Link™ Plus Activated Peroxidase Kit (ThermoFisher) and detected with ECL (Pierce ...
-
bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
-
bioRxiv - Cell Biology 2019Quote: ... and 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) (Invitrogen) in water for 1 hour at 60°C ...
-
bioRxiv - Neuroscience 2019Quote: ... Fluo-3-acetoxymethylester (Fluo-3-AM) (Molecular Probes-Thermo Fisher Scientific) was used (Beauvais et al. ...
-
bioRxiv - Neuroscience 2019Quote: ... Fluo-3-acetoxymethylester (Fluo-3-AM) (Molecular Probes-Thermo Fisher Scientific) was used (Beauvais et al. ...
-
bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
-
bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
-
bioRxiv - Immunology 2022Quote: ... 1.9g of ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (ThermoFisher #22980) and 1.2g of N-hydroxysuccinimide (NHS ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
-
bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
-
bioRxiv - Physiology 2021Quote: ... 100 μL of One-step Ultra TMB ELISA substrate (Thermo Fisher Scientific, 34028) was added to each well and incubated for 15 min at RT ...
-
bioRxiv - Developmental Biology 2021Quote: ... 100 ml of 1-Step-Ultra TMB-ELISA Substrate (Thermo Fisher, cat#34028) was pipetted to each well and incubated for 30 minutes in dark at room temperature ...
-
bioRxiv - Immunology 2021Quote: For polyreactivity ELISAs against human insulin (MilliporeSigma) and dsDNA (Calf Thymus DNA; Invitrogen), plates were coated with 2µg/ml and 50µg/ml ...
-
bioRxiv - Immunology 2021Quote: Levels of anti-dsDNA IgG were measured by ELISA using MaxiSorp plates (Nunc) coated with dsDNA from calf thymus (20 µg/ml ...
-
bioRxiv - Microbiology 2021Quote: ... were coated onto wells of an uncoated 96-well ELISA plate (Nunc Maxisorp) overnight at 4°C in coating buffer ...
-
bioRxiv - Microbiology 2021Quote: Quantification of IL-1β secretion in cell supernatant was measured by ELISA (Invitrogen, IL-1 beta Mouse Uncoated ELISA Kit ...
-
bioRxiv - Microbiology 2020Quote: ... 96-well ELISA plates (Nunc Maxisorp or Nunc Immobilizer Amino Plates, ThermoFisher, USA) were incubated with 1.2 μg of each peptide diluted in 100 μl of phosphate-buffered saline (PBS ...
-
bioRxiv - Microbiology 2022Quote: ELISA: Immulon 4 HBX high binding clear flat bottom 96 well plates (ThermoFisher) were coated with SARS-CoV2 Spike RBD319-591-Fc fusion at 5 μg/ml in 1xPBS overnight at 4 °C followed by washing ...
-
bioRxiv - Biochemistry 2022Quote: ... and 200μl of tetramethylbenzidine (TMB) ELISA substrate (Thermo Fisher Scientific; cat. no. 34028) was added to each well ...
-
bioRxiv - Microbiology 2021Quote: ... Colorimetric changes were measured using an ELISA microplate reader (MULTISKAN FC, Thermo Scientific) at 450 nm ...
-
bioRxiv - Microbiology 2020Quote: ... and PDGF-D by enzyme-linked immunosorbent assay (ELISA, TGF-β: Thermo Fisher Scientific ...