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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for HER3 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and p976 were packaged in HEK-293T cells at a ratio of 2:1:1 by Lipofectamine 2000 (Invitrogen). The ratio of liposome to DNA was 2:1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human Embryonic Kidney (HEK)293T cells (ATCC) were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (Bodinco and Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension HEK293S GnTI− cells expressing dolphin Prestin were grown at 37 °C and 7.8% CO2 in t FreeStyleTM 293 expression medium (Gibco Fisher Scientific) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: GAL4–PAX6 was cloned into the pcDNA5/FRT/TO expression vector by standard procedures and subsequently used for recombination into the genomic DNA of TREx 293 Flp-In cells (Invitrogen) as described in details previously41 ...
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bioRxiv - Molecular Biology 2019Quote: Human embryonic kidney 293 T (HEK293T) cells were maintained in MEM/EBSS (HyClone) supplemented with 10% fetal bovine serum (FBS, Gibco), 100U/ml penicillin and 100μg/ml streptomycin (Lonza) ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified cosmid DNA (2 μg) was digested with Pac1 and transfected into 293 cells with Lipofectamine 2000 (ThermoFisher 11668019). Adenovirus plaques appeared 7 days after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... P2 or P3 baculovirus was produced in Sf9 cells and used to transfect HEK293S GnTI- suspension cells cultured in Freestyle 293 (Gibco) with 2% FBS at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2020Quote: All ectodomains were produced in 500mL cultures of HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m. ...
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bioRxiv - Microbiology 2021Quote: ... whilst the growth medium of T-REx-293- derived cell lines stably transfected with pcDNA4/TO-based plasmids was further supplemented with zeocin (100 µg/mL, Gibco).
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bioRxiv - Biochemistry 2021Quote: The knock-out cell line (GTPBP5KO) was generated in the Flp-In T-Rex human embryonic kidney 293 (HEK293T) cell line (Invitrogen) using CRISPR/Cas9 technology targeted on exon 1 of MTG2 gene ...
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bioRxiv - Biochemistry 2020Quote: HEK293F cells were seeded in 24 well plates (1 million cells in each well) in a final volume of 1 mL of FreeStyleTM 293 medium (GIBCO). The cells were transfected with 1 ug DNA as described before and incubated at 37°C and in 8% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... these cells were adapted to suspension culture by growing the cells in a mix of FreeStyle(tm) 293 Expression medium (Gibco) and DMEM/FCS and decreasing the concentration of DMEM/FCS at each passage ...
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bioRxiv - Immunology 2021Quote: ... 50μg of the plasmid was then transfected into Expi293F cells at a density of 3×106 cells/mL in a 250mL shaking flask using the ExpiFectamine 293 Transfection Kit (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells that express mNRK in a dox-dependent manner were developed using Flp-In T-REx 293 cells (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... n372361, n372362) or Negative Controls (4390843, 4390846) and transfected into 293 cell lines using Lipofectamine RNAiMAX Transfection Reagent (13778030, ThermoFisher). Transfections were performed in a 24-well plate with 5 pmol of siRNA and 1.5 μl RNAiMAX Transfection reagent per well ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells stably expressing FLAG/HA-tagged human PRPF4 was generated using the pcDNA5/FRT-PRPF4 plasmid and Flp-In-293 cells according to the manufacturer’s protocol (Thermo Fisher). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Flp-In-293 cells were transfected with a 9:1 ratio of pOG44:pcDNA5/FRT-PRPF4 plasmids using Lipofectamine 2000 (Invitrogen) and 48 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was expressed in FreeStyle 293-F cells (Invitrogen). The plasmid was transiently transfected at a density of 2 × 106 cell per ml using polyethyleneimine (PEI ...
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells were seeded in 24-well plates (1 million cells in each well) in a final volume of 1 ml of FreeStyleTM 293 medium (GIBCO). The cells were transfected with 1 µg DNA of different hSARM1 constructs and incubated at 37°C and in 8% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... mAbs were produced by transient transfection into Expi293F™ cells with equal amounts of paired heavy- and light-chain expression vectors using ExpiFectamine 293 transfection kit according to the manufacturer’s protocol (Thermo fisher). Five days post antibody expression ...
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bioRxiv - Biochemistry 2022Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). Plasmids were transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Microbiology 2021Quote: HEK293T-ACE2 cells were reverse-transfected with plasmids and TransIT-293 at a ratio of 1 µg DNA : 3 µLTransit-T293 in Opti-MEM (Gibco). For an experiment conducted in triplicate at a 48-well scale ...
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bioRxiv - Molecular Biology 2020Quote: ... and pcDNA5/FRT/TO-based plasmids carrying the GFP-RIF1-L or GFP-RIF1-L-pp1bs gene were mixed in 9:1 molar ratio and used for transfection of Flp-In T-Rex 293 cells (Invitrogen) with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells grown to a density of 2.5 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for two days at which point the supernatant was harvested ...
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bioRxiv - Biochemistry 2020Quote: A 100 ml culture (1 × 106 cells/ml) of 293F cells were transfected with 100 µg of pCMV-FLAG-His6-NPC1L1-MLD plasmid using 293-fectin (Invitrogen) according to the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: NiV prefusion glycoprotein and avi-tagged probes were expressed by transfection in 293 Freestyle (293F) cells (Thermo Fisher Scientific, MA) using Turbo293 transfection reagent (SPEED BioSystem ...
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bioRxiv - Immunology 2020Quote: ... YP_009724390.1) ectodomain was produced in 500mL cultures of HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m ...
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bioRxiv - Physiology 2020Quote: ... stable inducible cell lines expressing WT or mutant EAAT2 were generated by selection of Flp-In T-Rex 293 cells (Invitrogen) transfected with pcDNA5/FRT/TO-EAAT2 and used either with or without induction with tetracycline (38,50,52).
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bioRxiv - Microbiology 2021Quote: ... Transfections were carried out using 1 mg of total DNA (500 μg each of VH and Vκ plasmid DNA) and 1 l of cultured HEK293-F cell suspension maintained in sterile Freestyle 293 expression medium (Invitrogen) without antibiotics at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using pCMV::SARS-CoV-2_S_NTD derivative mutants with the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) with and cultivated for five days at which point the supernatant was harvested ...
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bioRxiv - Immunology 2019Quote: ... 1 μg/mL puromycin and expanded after adapting cells in suspension to Expi-293 medium (Life Technologies, Grand Island, NY) supplement with 1 μg/mL puromycin ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney 393T cells were adapted to suspension culture in Gibco Freestyle 293 medium (Thermo Fisher Scientific, Waltham USA) supplemented with 20 U/L penicillin ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney cells adapted to suspension culture (HEK293F) were grown in Free Style 293 Expression Medium (Thermo Fisher Scientific) and harvested at a density of 3 million cells/ml ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 2.5 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Microbiology 2020Quote: ... and HEK293S GnTI-/- cells (ATCC CRL-3022) in Freestyle 293 Expression Medium supplemented with 2% ultra-low IgG FBS (Gibco). We maintained ...
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bioRxiv - Biochemistry 2022Quote: ... RBD of SARS-CoV2 and SARS-CoV S were transiently expressed in FreeStyle™ 293-F cells (Thermo Fisher Scientific). Cells were resuspended at 1×106 per mL with fresh media on the day of transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... sequence confirmed plasmid DNA was diluted in OptiMEM I serum-free medium and incubated for 20 minutes with ExpiFectamine 293 Reagent (Gibco). The DNA– ExpiFectamine 293 Reagent complexes were used to transfect Expi293F cell cultures in 96-deep-well blocks ...
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bioRxiv - Immunology 2022Quote: All SARS-CoV-2 S ectodomains were produced in 500-mL cultures of FreeStyle™ 293-F cells (ThermoFisher Scientific) grown in suspension using FreeStyle 293 expression medium (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with pcDNA4TO HMM-HA-Spike and pcDNA tagBFP for transfection marker using ExpiFectamine 293 (Thermo Fisher Scientific). Cells were centrifuged at 200 g for 2 min 48 h after transfection and washed with Dulbecco’s phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... with a secretion sequence and C-terminal His6-tag) were transiently expressed using FreeStyle™ 293-F cells (Thermo Fisher) in FreeStyle™ F17 Expression Medium supplemented with L-glutamine and 1X MEM non-essential amino acids (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... plasmids encoding for various SCTs were co-transfected with a plasmid encoding for an ER-retained BirA-ligase into FreeStyle 293-F cells (Invitrogen) using the 293-free transfection reagent (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... Y-A or YIY-AAA) and FLAG-tagged CDK-1 constructs were expressed in FreeStyle 293-F cells (ThermoFisher Scientific). Cells were transfected using FreeStyle MAX Reagent and OptiPRO SFM according to the manufacturer’s guidelines (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... were transfected with expression vectors for 3xFLAG-tagged wt eIF3g or its mutated forms using Expifectamine 293 according to the manufacturer’s protocol (Thermo Scientific). Cells expressing 3xFLAG-tagged eIF3g (200 ml suspension ...
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bioRxiv - Biochemistry 2023Quote: The WDR76 knockout cell line was derived from Flp-In™-293 Cell Line purchased from Invitrogen (Carlsbad, CA, USA). The knockout was achieved using RNA-guided CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas9 system (51) ...