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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 6 TRIFLUOROMETHYL 1 2 3 4 TETRAHYDRO ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged 1:6 once at 70% confluency using Versene (Gibco). Monocyte factories were set up following a previously reported protocol (van Wilgenburg et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining material was resuspended in 6 mL sterile 1× PBS (Gibco). Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter) ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:6 in DMEM/F12 media (11320033, Thermo Fisher, Waltham, USA). The iPSCs were maintained ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged 1:6 once at 70% confluency using Versene (Gibco). Monocyte factories were set up following a previously reported protocol (van Wilgenburg et al. ...
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bioRxiv - Biophysics 2020Quote: ... Grids were blotted for 2-4 seconds at a blotting force of 4 and plunge-frozen in liquid ethane using a MarkIV Vitrobot (Thermo Fisher Scientific). The chamber was maintained at 8 °C and 100% humidity during freezing ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was kept after spinning at 800 rcf for 2’ at +4°C and were incubated overnight at +4°C with 3µg of dedicated antibodies: Nr2f1 antibody (Thermo Fisher PA5-21611) and GFP antibody (Abcam ab13970 ...
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bioRxiv - Immunology 2020Quote: ... 2-4 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded with the calcium indicator Fluo-4 by incubating in supplemented NB medium that contained 2 µM of Fluo-4 AM (Invitrogen, Carlsbad, CA), and Pluronic F-127 (0.04% ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated for 2–3 min at RT in trypsin (0.05% v/v; Gibco 25300-054), and tapped to release ...
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bioRxiv - Cancer Biology 2020Quote: ... and 12 mM 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) (Thermo Fisher Scientific, #M6494). After 4 hours ...
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bioRxiv - Biochemistry 2020Quote: ... and SARS-CoV-2 [3]) was performed with Proteome Discoverer version 2.4 (Thermo Fisher Scientific). The search was restricted to human and SARS-COV-2 proteins ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μg of RNA was digested with Turbo DNase (2 U/μl, # AM1907, ThermoFisher Scientific). 500 ng of DNase-digested RNA was tagged with G/I tails in a 10 μl reaction ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated for 3 days in presence of 100 U/mL IL-2 (Gibco) and 1 µg/mL PHA (Remel).
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bioRxiv - Microbiology 2020Quote: ... The modified nucleotide 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated by PCR using DreamTaq™ DNA Polymerase (Thermo Scientific™) ...
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bioRxiv - Microbiology 2021Quote: ... or with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) for 30 min at room temperature (1:150 in AnnexinV binding buffer-Biolegend) ...
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bioRxiv - Microbiology 2021Quote: ... and 2-3 cm) after measuring the DNA concentration with Qubit (QubitⓇ 2.0 Fluorometer, Invitrogen). We obtained eight metagenomes from Lake Sarnen (deepest and shallow_inflow station for March ...
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bioRxiv - Evolutionary Biology 2022Quote: ... where an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles; Thermo Fisher) pre-column was connected in front of an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Molecular Biology 2019Quote: ... an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles, Thermo Fisher Scientific) pre-column was connected in front of an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Microbiology 2019Quote: ... Primer 2: 5’-GGATGTTCGTCCAGTGAGATTAG-3’) using a 7500 Fast Real-Time PCR System (Applied Biosystems) and accompanying software to analyze qPCR data.
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bioRxiv - Microbiology 2021Quote: ... an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)) assay (M6494, Thermo Fisher Scientific) was conducted in parallel to the infection and kinase inhibitor treatment according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were prepared by incubating with a 2% solution of 3-aminopropyltrimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Biochemistry 2021Quote: ... a pEntryslot3 vector containing the let-858 or tbb-2 3’UTR and pDEST (ThermoFisher) in an LR reaction ...
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bioRxiv - Biophysics 2019Quote: ... Medium was changed every 2-3 days and cells were harvested with TrypLETM Express (Invitrogen) at 80%-90% confluency ...
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bioRxiv - Cell Biology 2021Quote: siRNA transfections were performed 2 or 3 days before each experiment using Lipofectamine RNAiMax (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... All cell lines were passaged every 2-3 days using 0.05% Trypsin/EDTA solution (Gibco).
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were prepared by incubating with a 2% solution of 3-aminopropyltrimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Genomics 2022Quote: ... The remaining RNA (< 3 µg) was treated with 2 U TURBO™ DNase (Invitrogen, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Samples were DNase treated (3 µL of Turbo RNase-free DNase, 2 U/µL, ThermoFisher) for 20 min at 37°C and then precipitated by addition of 290 µL of water ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were subcultured every 2-3 days and transfected with lipofectamine 2000 transfection reagent (Invitrogen) in Opti-MEM medium (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were aspirated and 2-3 crystals of Dil stain (Life Technologies-Molecular Probes) were added to each culture well and incubated on a shaker for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were aspirated and 2-3 crystals of Dil stain (Life Technologies-Molecular Probes) were added to each culture well and incubated on a shaker for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Passaging was performed every 2 to 3 days using Accutase (00-4555-56, ThermoFisher Scientific). The cells were Mycoplasma tested prior to running experiments ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was carefully removed and resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 20 min at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 ng equivalent was synthesized using Super Script 3 Supermix (18080400, Thermo Fisher, USA) with stem-loop primers and sly actin_R primers as internal controls (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged every 2–3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer ...
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bioRxiv - Genomics 2024Quote: ... Cells were passaged every 2-3 days by detachment with 0.05% trypsin-EDTA (ThermoFisher Scientific).
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bioRxiv - Genomics 2023Quote: ... mESCs were routinely passaged at ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher). Before experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... passage 2-3 NPCs were detached with Stempro Accutase cell dissociation reagent (A1110501, Life Technologies) and kept in cold PBS 1%FBS for the duration of the protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Cytotoxicity was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (ThermoFisher: M6494) reduction analysis according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Organoid images were digitally acquired every 2–3 days on an EVOS M7000 (ThermoFisher, AMF7000) with DiamondScope software (version 2.0.2094.0 ...
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bioRxiv - Immunology 2024Quote: ... 10% heat inactivated fetal bovine serum (HIFBS) 2 × 10-3 m L-glutamine (Life Technologies), antibiotic antimycotic (1× ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Molecular Biology 2022Quote: ... pDNOR207-ZNF451-1 (isoform 1) and pDNOR207-ZNF451-3 (isoform 3) were generated using the Gateway® cloning BP reaction (Thermo Fisher Scientific) upon cDNA amplification using BP-tailed primers and pDNOR207 as donor vector ...
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bioRxiv - Microbiology 2021Quote: ... and 40 µg/mL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Thermo Scientific). Plates were incubated at room temperature for 48-72 h ...
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bioRxiv - Cell Biology 2019Quote: ... For differentiation cells were switched to M254 medium for 3-4 population doublings (ThermoFisher Scientific, Life Technologies).
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bioRxiv - Cell Biology 2019Quote: ... For differentiation cells were switched to M254 medium for 3-4 population doublings (ThermoFisher Scientific, Life Technologies).
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bioRxiv - Bioengineering 2020Quote: Before staining with either 5-bromo-4-chloro-3’-indolyphosphate and nitro-blue tetrazolium (BCIP/NBT, ThermoFisher) or Alizarin Red S (ARS ...
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bioRxiv - Neuroscience 2020Quote: ... on a 4-12% bis-tris gel (Genescript) in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). The gel was then fixed for 30 min in 10% acetic acid/50% methanol ...
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bioRxiv - Cell Biology 2021Quote: ... For live imaging of cells as described for fatty acids up-take we treated cells at day 5 of differentiation with BODIPY™ (10μM) C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen). In addition ...