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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 6 Fluoro 1 3 benzodioxene 8 carbonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... mRNAs were synthetized by in vitro transcription with 1μl of GTP added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Developmental Biology 2021Quote: ... Each plasmid was linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription with 1 µL of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Biophysics 2022Quote: ... 0.020 M potassium phosphate monobasic), sodium chloride (BioXtra grade, purity ≥99.5%) and D-(-)-Fructose (HPLC grade, purity ≥99%) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μM gramicidin was added to the internal recording solution to induce chloride-impermeable pore formation along with 25 μM Alexa Fluor 568 hydrazide Na+ salt (Invitrogen) to visualize any potential pore rupture ...
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bioRxiv - Immunology 2022Quote: ... and red blood cells of fresh spleens or bone marrow were lysed in ammonium-chloride-potassium buffer lysing buffer (Gibco) for 3 min ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 1500 rpm for five minutes and then resuspended in 5mL of Ammonium-Chloride-Potassium (ACK) lysis buffer (Gibco) on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The pellet was resuspended in 200–500 µl of ACK (ammonium-chloride-potassium) RBC lysis buffer (Thermo Fisher Scientific, A1049201) and incubated for 1 min on ice ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma HCT-8 cells (ATCC) and THP-1 cells (ATCC) were maintained in Advanced RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1X penicillin-streptomycin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 3.2 μg of p0G44 (pSB2380) and 1 μg of pSB2860/pSB2863 were mixed with 8 μL of Lipofectamine2000 (Invitrogen 11668027) in 400 μL of DMEM (no supplements ...
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bioRxiv - Molecular Biology 2021Quote: 1×104 A549 cells were grown in poly(D-lysine)-coated borosilicate glass Lab-Tek 8-well chambers (Thermo Scientific) and stained with MitoTracker Green FM (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37C incubator (ThermoFisher). Prior to plating ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). For σ28 purification ...
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bioRxiv - Cell Biology 2021Quote: ... dried peptide sample was resuspended in 200 mM HEPES (pH 8) and incubated for 1 h at room temperature with one of the TMT10-plex reagents (ThermoFisher) solubilized in 100% anhydrous ACN ...
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bioRxiv - Microbiology 2021Quote: 8 x 103 HeLa-ACE2 cells and 3.2 x 104 HeLa or HeLa-sgLRRC15 cells (1:4 ratio) were co-plated per well in 8-well chamber slides (Nunc). The following day ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... 1×104 transfected cells were seeded into each well of 8-well Lab-Tek™ II chamber slides (ThermoFisher Scientific) for immunostaining analysis ...
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bioRxiv - Biochemistry 2022Quote: ... coated Corning dishes at 37°C in a 5% CO2 incubator and gently dissociated for passaging at 1:8 ratio every second day with Stem Pro Accutase (Gibco). Cells were washed with Dulbecco’s Phosphate Buffer Saline cell culture grade.
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bioRxiv - Microbiology 2021Quote: ... HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day or more prior to infection ...
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bioRxiv - Immunology 2021Quote: HEK203-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day or more prior to infection ...
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bioRxiv - Immunology 2020Quote: HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day prior to infection ...
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Genomics 2021Quote: ... The tubes were then supplemented with 630 μl chloroform/isoamyl alcohol (24:1, pH 8, Acros Organics, Cat. No.: 327155000) and bead beat at setting 6 for 40 sec using the FastPrep-24™ Classic Instrument (MP Biomedicals ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples at the Cambridge laboratory were stained with a 1:2500 dilution (8 µM) of Hoechst 33342 (peak excitation: 351 nm, peak emission: 461 nm) (#62249, ThermoFisher) for 30 mins ...
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated in 20 mL 33 loading buffer diluted to 13 in lysis buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific)) ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resuspended in 500µL of Sonication buffer (SDS 1%, 10mM EDTA, 50mM Tris HCl pH 8) with an anti-protease cocktail (A32965, ThermoFisher) and sonicated for 15” at 4°C (30s ON ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mL Lysis Buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific), and a 45-s process in a FastPrep-24 (MP Biomedicals ...
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bioRxiv - Neuroscience 2023Quote: ... SARK and HMW tau extracts (8 ng total tau per well) were incubated with 1% lipofectamine 2000 transfection reagent (ThermoFisher) in opti-MEM (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... medium containing 2 µg/ml puromycin and 8 µg/ml polybrene (HCT116-Cas9) and 1 µg/ml (iPSC-iCas9) or no (NPCs- iCas9) puromycin (GIBCO) was exchanged ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fed in full media for 1 h prior to 8 h starvation in Earle’s Balanced Salt Solution (EBSS; Life Technologies).
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bioRxiv - Biochemistry 2023Quote: ... 200 mM Tris-HCl, pH 8.0, 8 M Urea, 4% CHAPS, 1 M NaCl, supplemented with protease and phosphatase inhibitors, Thermo Scientific) was added to the pellets and the extracts were sonicated 10 x 3s with a probe sonicator ...
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bioRxiv - Biophysics 2023Quote: ... Then we detached them using trypsin and plated a 1:10 dilution in DMEM on an 8-well chamber (Lab-Tek 1.0 borosilicate glass, Thermo Fisher), which we let grow overnight ...
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bioRxiv - Microbiology 2023Quote: Cells were resuspended in PBS and incubated at 4 °C for 5 mins with 8 µg ml−1 Alexa Fluor 555 NHS ester (Invitrogen). Cells were then washed in PBS.
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bioRxiv - Cancer Biology 2023Quote: ... Stably transduced cell lines were generated by infection over-night in the presence of 8 μg/ml polybrene and 48 hours after infection were selected with 1 μg/mL Puromycin (Gibco) for three days ...
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bioRxiv - Physiology 2023Quote: Animals were culled using a schedule-1 method followed by injection of the common bile duct with 1 mg/mL collagenase NB 8 (Serva) in RPMI 1640 (Gibco) and pancreas dissection ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher) and loaded onto a MonoQ 5/50 GL column for anion exchange chromatography ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher), then concentrated to ∼0.5 mL and loaded onto a Superose 6 Increase 10/300 column pre-equilibrated in GF150 buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Larval zebrafish at 6-7 dpf were paralyzed by immersion in 1 mg/ml alpha-bungarotoxin solution (Invitrogen) dissolved in external solution (in mM ...
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... INS-1 cells were transfected in 6-well plates with 5 nM siRNA using Lipofectamine RNAiMAX (Invitrogen, 13778030) and typically analyzed after 96 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1:5000 in PBS) (Molecular Probes, Eugene, OR). The TUNEL stain signal was observed under an FV300 confocal microscope (Olympus Optical Company ...
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bioRxiv - Molecular Biology 2019Quote: ... Aliquots were solubilized in 1 mL 8M urea lysis buffer: 8M Urea (Invitrogen, CA; CAS# 57-13-6), 100mM NaCl(Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Desiccated samples were subjected to 5-6 rounds of desiccation and rehydration (1-hour speedvac desiccation [Thermo Fisher Scientific] followed by resuspension in water) ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Microbiology 2021Quote: ... L454W/E455G and S262R) and mCherry (6:4:1 mixtures; 2.2 μg/well) using Lipofectamine® 2000 (Invitrogen), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... Mice were euthanized by CO2 asphyxiation at 6 h and lungs placed in 1 ml TRIzol (Thermo Fisher). RNA was then extracted using acid-phenol-chloroform-isoamyl alcohol (125:21:1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were seeded in 6-well plates in 1 ml of RPMI 1640 medium (Gibco, Amarillo, TX, USA) supplemented with 10% fetal bovine serum and 0.2% primocin (Invivogen ...
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bioRxiv - Molecular Biology 2021Quote: ... HL-1 cells in 6-well plates were transfected with 15 µL Lipofectamine 2000 reagent (Life Technologies 11668019) complexes with nucleic acid ...
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bioRxiv - Genomics 2020Quote: ... the cells were split 1:6 with Accutase (StemPro® Accutase® Cell Dissociation Reagent, Gibco™ A1110501), onto matrigel substrate in the presence of 10 μM of rock inhibitor (Y-27632 dihydrochloride ...