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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... peroxidase substrate (Life Technologies) was added and after 10 min plates were read at 405 nM on an Emax plate reader (Molecular Devices) ...
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bioRxiv - Immunology 2020Quote: IL-2/Fc fusion proteins were expressed using the Expi293 expression system according to manufacturer instructions (Thermo Scientific). Proteins were as human IgG1 Fc fused at the N-or C-terminus to human IL-2 through a (G4S)4 linker ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (AlexaFluor488 donkey anti mouse IgM 1/400, Life Technologies in Buffer A, 0.1% Saponin 2% FCS) was performed ...
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bioRxiv - Immunology 2020Quote: ... and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104). Labeled cells were acquired using a Thermo-Fisher Attune NxT flow cytometer and analyzed using Flowjo Software.
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2.
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bioRxiv - Immunology 2020Quote: ... and lymphocytes were resuspended in complete RPMI (cRPMI- RPMI + 10% FCS + 2% Penicillin-Streptomycin + 1% L-glutamine (Gibco)) ...
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bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Bones were flushed using a 26-gauge needle of a 1-ml syringe with HBSS+2% FCS (Gibco). BM clumps were re-suspended by gentling aspirating with a 19-gauge needle ...
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bioRxiv - Immunology 2022Quote: ... These cells were cocultured with A549-ACE2-A2402 cells expressing each spike protein an E:T ratio of 2:1 and incubated with RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS at 37°C for 6 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of RNA was used as input with 2 μL of a 1:100 dilution of ERCC RNA Spike-In Mix (ThermoFisher Scientific, catalog 4456740) and the KAPA RNA Hyper + RiboErase HMR kit was used (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Reporter-expressing rSARS-CoV-2 were detected directly by epifluorescence and using either Alexa Fluor 594 goat anti-mouse IgG (Invitrogen; 1:1,000) or fluorescein isothiocynate (FITC)- conjugated goat anti-mouse IgG (Dako ...
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bioRxiv - Biophysics 2020Quote: Phase diagrams of non-phosphorylated and phosphorylated NSARS-CoV-2 at different concentrations were determined using a NanoDrop spectrophotometer (ThermoFisher Scientific, Invitrogen). Increasing concentrations of polyU (0 - 2 µM ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of purified SARS-CoV-2 Mpro was determined by the absorbance at 280 nm (A280) using a NanoDrop 2000c UV-Vis spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... was used as soluble substrate to detect SARS-CoV-2 S-protein specific monoclonal antibodies and the final reaction was measured by using the Varioskan Lux Reader (Thermo Fisher Scientific) at a wavelength of 405 nm ...
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bioRxiv - Microbiology 2021Quote: ... and assembled into full length SARS-CoV-2 cDNA clones in the YAC vector pYESL1 using a GeneArt™ High-Order Genetic Assembly System (A13285, Invitrogen™, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... was used as soluble substrate to detect SARS-CoV-2 S-protein specific monoclonal antibodies and the final reaction was measured by using the Varioskan Lux Reader (Thermo Fisher Scientific) at a wavelength of 405 nm ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 antibody (Antibodies Online; catalog no. ABIN6952432) followed by staining with secondary antibody Alexa-647 (goat anti-mouse; Thermo Fisher; A21235) and DAPI for nuclei staining ...
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bioRxiv - Neuroscience 2022Quote: ... Donkey-anti-Sheep 647 (1:1000, Thermo Fisher Scientific, A-21448), Goat anti-Chicken IgY (H+L ...
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bioRxiv - Neuroscience 2019Quote: ... with the magnetic sheep anti-rabbit beads (Invitrogen Corp., Carlsbad, CA) at 4°C overnight on a rotator ...
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bioRxiv - Cell Biology 2021Quote: ... was incubated with sheep anti-rat IgG magnetic dynabeads (Invitrogen, 11035) in a solution of sterile PBS 0.1%BSA (120ul of beads ...
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bioRxiv - Neuroscience 2020Quote: ... and Alexa Fluor 568 donkey anti-sheep IgGs (1:200, Invitrogen). Sections were counterstained using bis-benzamide (1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by incubation with Alexa Fluor 555 donkey anti-sheep (Invitrogen) and Alexa Flour 488 goat anti-mouse (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... donkey-anti-sheep AF 488 (Thermo Fisher # A-11015, 1:500); donkey-anti-mouse AF 488 (Thermo Fisher #A-21202 ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 555 donkey-anti-sheep IgG (1:500; A21436, Invitrogen).
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bioRxiv - Biochemistry 2022Quote: 300 μl M280 Dyna beads sheep anti-rabbit IgG (Life technologies) was added to 1 ml freshly prepared PBS with 5 mg/ml BSA (PBS/BSA ...
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bioRxiv - Developmental Biology 2019Quote: ... amplified with anti-sheep Alexa Fluor 488 (1:100, Molecular Probes) and biotinylated anti-Avidin (1:100 ...
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bioRxiv - Immunology 2020Quote: ... 0.5 ml defibrinated sheep blood (Thermo Scientific Oxoid, catalog no. SR0051D) was added to deplete T cells in the next step ...
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bioRxiv - Immunology 2020Quote: ... After blocking with 100 μl of sheep serum (16070-096, Gibco), the sera from COVID-19 patients were serially diluted from 10- to 31,250-fold in blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... and sheep IgG (1:500 for IF) were from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... GR1 and MAC1 together with sheep anti-rat IgG Dynabeads (Invitrogen) prior to staining with fluorescent antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... Epithelial cells were isolated using sheep anti-rat Dynabeads (Thermo Fisher) with rat anti-mouse CD326 antibody (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... or Alexa Fluor 680-conjugated anti-sheep IgG (Thermo Fisher Scientific). Biotin was detected using streptavidin-Alexa Fluor 680 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... coupled to magnetic beads (Dynabeads™ Sheep Anti-Rat IgG, ThermoFisher), and cultured as indicated in M199 supplemented with 20% FBS ...
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Hepatic FGF21 mediates tissue tolerance during bacterial inflammation by preserving cardiac functionbioRxiv - Immunology 2020Quote: ... plated on Blood Agar (TSA with 5% Sheep Blood) plates (ThermoFisher), and incubated at 37°C for 18 hours.
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bioRxiv - Genomics 2022Quote: ... coated Dynabeads M-280 (Sheep anti-Rabbit Thermo Scientific cat #11201D) and rotated at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... 20 uL DynabeadsTM M-280 Sheep Anti-Rabbit IgG (Invitrogen, #11203D) suspension per sample was blocked with freshly prepared 0.5% BSA at 4°C for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with Alexa Fluor 555 donkey anti-sheep (Invitrogen) and Alexa Fluor 488 donkey anti-mouse (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Columbia agar plates with 5% sheep blood (Thermo Fisher Scientific, PB5039A) were streaked with the corresponding glycerol stocks ...
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bioRxiv - Microbiology 2024Quote: ... tryptic soy agar with sheep’s blood (pre-made plates: Thermo Scientific; tryptic soy agar ...
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bioRxiv - Cancer Biology 2024Quote: ... and donkey anti-Sheep Alexa Fluor 594 (Invitrogen, cat#A-11016). The sections were mounted with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2023Quote: ... and the Sheep Anti-Rat IgG Dynabeads (Thermo Fisher Scientific, 11035) were used for pull-down assay ...
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bioRxiv - Immunology 2023Quote: ... and Dynabeads™ sheep anti-rat IgG beads (Invitrogen, MA, USA) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... and anti-sheep AlexaFluor 647 (A-21448, Thermo Fisher, RRID: AB_2535865).
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bioRxiv - Cancer Biology 2024Quote: ... Dynabeads™ M-280 Sheep Anti-Rabbit IgG (Invitrogen™, 11203D), Dynabeads™ MyOne™ Streptavidin C1 (Invitrogen™ ...
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Microbiology 2021Quote: ... and probed with DNA oligonucleotide (Table S1) in OligoHyb buffer (Thermofisher) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Custom primers were purchased from Sigma and miRNA probes were from Life Technologies (Supplementary Methods, Table S1).
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bioRxiv - Neuroscience 2021Quote: ... three different linkers were synthesized (Table S1, GeneArt Invitrogen, codon optimized) and inserted into GCaMP8s ...
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bioRxiv - Cell Biology 2021Quote: ... S1 C (Pierce Unstained Protein MW Marker from Thermo Scientific, 26610) and Fig ...