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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for Podocalyxin PODXL Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... incubated with primary antibody overnight followed by washing and incubation with species-appropriate secondary antibody at 1:200 (Alexa Fluor 555, Invitrogen) for at least 2 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was incubated in a corresponding primary antibodies in a 1/1200 dilution and polyclonal anti- Mouse IgG antibody from goat (Invitrogen) as a secondary antibody at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Coverslips were washed 3 times for 5 min each with PBS and incubated with 1:10,000 dilution Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 568 secondary antibody (#A-11036, Invitrogen) for 1 hour at room temperature in the dark ...
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bioRxiv - Bioengineering 2019Quote: ... Western blot was conducted using the Tau-13 antibody (dilution rate 1:2,000) followed by an anti-mouse IgG antibody conjugated to HRP (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with anti mTOR antibodies 1:200 overnight at 4°C and processed as above using anti-rabbit secondary antibody Alexa 555 (Invitrogen) at 1:400 in 3% BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... and finally stained with the anti-sHP1γ antibody (1:250) and Alexa Fluor 488 anti-rabbit secondary antibody (1:500, Invitrogen). Coverslips were mounted in VectaShield mounting media with DAPI for immunofluorescence ...
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bioRxiv - Genetics 2020Quote: ... samples were washed three times in PBT for 15 min and incubated for 2 h at room temperature with secondary antibody (antibodies coupled to Alexa fluorophores, Invitrogen) diluted 1:600 in PBT containing Hoechst dye ...
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bioRxiv - Immunology 2021Quote: ... Expanded transduced HAP1.MR1 D9 cells were thawed and approximately 3×109 cells were pulsed with 5 µg/ml Ac6FP for 5 h before cell surface staining with anti-MR1 primary antibody (clone 26.5) and goat anti-mouse secondary antibody coupled to Alexa488 (Life Technologies). Stained cells were fixed ...
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bioRxiv - Immunology 2021Quote: ... Virus-infected cells were detected using in-house raised rabbit anti-S polyclonal antibody along with peroxidase-conjugated goat anti-rabbit IgG (H+L) secondary antibody (Invitrogen). MN titers represented the reciprocal of the highest serum dilution that yielded >50% reduction in OD values as compared to wells containing virus only ...
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bioRxiv - Neuroscience 2021Quote: ... We incubated first with the 6H4 anti-PrP antibody (1: 1,000 dilution) followed by the secondary antibody anti-mouse- Cy3 (Molecular Probes) at 1:600 dilution ...
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bioRxiv - Microbiology 2021Quote: ... The immunoblots were analyzed using primary antibodies incubated overnight at 4°C and secondary antibodies linked to horseradish peroxidase (HRP) (Invitrogen), and after each step immunoblots were washed 4x with TBST ...
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bioRxiv - Biochemistry 2021Quote: The Fab fragment of the ch14D5 antibody was prepared from the antibody using Immobilized Papain-agarose resin (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: ... and/or specifically labeled using the immunofluorescent antibodies Mouse CD9 Monoclonal (MEM-61)-Alexa Fluor® 647 antibody (Thermo Fisher, Catalog# MA5-18154 ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 1 hr staining at room temperature with secondary antibodies (goat anti-mouse alexa 488 antibody, Invitrogen A-11008), followed by 4x washes with 1% BSA in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were blocked in Odyssey blocking buffer for 30 min at room temperature and incubated with primary antibodies (Mouse anti-V5 antibody, Invitrogen R960-25 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in the same solution as the primary antibodies with the corresponding secondary donkey Alexa fluorescent antibodies (1:1000, ThermoFisher) for 2hr ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated surface GLT-1 transporters were identified by using anti-GLT primary antibody (1:500, Alomone) or anti-V5 primary antibody (1:2000, Invitrogen) and detection of enhanced chemilluminescence from HRP-coupled anti-rabbit secondary antibodies followed by detection with an ImageQuant LAS4000 mini imaging system and analysis with ImageQuant software (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... at 1:500 dilution and FITC-conjugated donkey anti-mouse secondary antibody and Cy3-conjugated goat anti-rabbit secondary antibody (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were detected using the fluorescent secondary antibodies goat anti-rabbit Alexa 488 (1:400, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse Alexa 546 (1:400 ...
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bioRxiv - Cell Biology 2021Quote: ... The viral spike protein was stained with an in-house developed rabbit anti-spike antibody and goat anti-rabbit IgG (H+L) highly cross-adsorbed antibody with Alexa Fluor 488 (Invitrogen). Images were acquired using FluoView FV10i Confocal Laser Scanning Microscope (Olympus) ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunohistochemistry was performed on serial sections with the following antibodies: cytokeratin pan antibody cocktail (ThermoFisher, Waltham, MA; catalog #MA5-13203), androgen receptor (AR ...
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bioRxiv - Cancer Biology 2020Quote: ... rinsed and incubated with secondary antibody Alexa Fluor 594 Goat Anti-mouse immunoglobulin G (IgG) (H/L) antibody (1:1000, Invitrogen) for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor-488-conjugated goat anti-mouse-IgG antibodies and Alexa-Fluor-594-conjugated goat anti-mouse-IgG antibodies (Thermofisher) were used.
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected by secondary anti-rabbit antibody conjugated with Alexa Fluor 488 and anti-goat antibody conjugated with Alexa Fluor 555 (Invitrogen), resulting in tdTomato protein being visible as red fluorescence and CRF peptide visible as green fluorescence ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with an anti-rabbit horseradish peroxidase-linked antibody for FtsZ and an antiguinea pig horseradish peroxidase-linked antibody for SepF (Invitrogen), both at 1:5000 dilution for 45 min ...
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bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... the slices were incubated in the secondary antibody Anti-Rabbit Alexa-647 (1:200 in Antibody buffer, Thermo Fisher A21244) at 4°C on a shaker for 2 days ...
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bioRxiv - Microbiology 2021Quote: ... the bound antibody was detected with goat anti-human Ig (γ chain specific)-HRP conjugated secondary antibody (Thermo Fisher Scientific) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... the goat anti-ACE2 primary antibody (R&D) and Alexa Fluor 568 donkey anti-goat IgG (H+L) secondary antibodies (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Pacific Blue (1:250, Molecular Probes P31582), goat anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibodies against binary toxin subunits CDTa and CDTb and anti-Clostridia GDH monoclonal mouse antibody (Clone: J44D, Invitrogen) were obtained from Merck ...
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bioRxiv - Microbiology 2019Quote: ... primary antibodies were diluted 1:100 and secondary antibodies conjugated to Alexa 488-or 568 (Invitrogen, Thermo Fisher Scientific, UK) were diluted 1:500 in a solution of BSA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... primary antibodies were diluted 1:100 and secondary antibodies conjugated to Alexa 488-or 568 (Invitrogen, Thermo Fisher Scientific, UK) were diluted 1:500 in a solution of BSA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... we probed the membranes sequentially with mouse anti-HuR (clone 3A2) and anti-actin primary antibodies (both from SantaCruz) and with goat anti-mouse AlexaFluor-680-conjugated secondary antibodies (ThermoFisher). We scanned the blots with LICOR Odyssey system and analyzed the intensity of the bands with ImageStudio Lite software version 5.2.
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bioRxiv - Cell Biology 2021Quote: ... The immunoblots were analysed using primary antibodies incubated overnight at 4°C and secondary antibodies linked to horseradish peroxidase (HRP) (Invitrogen), and after each step immunoblots were washed 4x with TBST ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-VE-cadherin antibody (1:100, Cell Signalling) followed by anti-rabbit Alexa 488 secondary antibody (1:1000, Invitrogen) to mark EC junctions.
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bioRxiv - Cell Biology 2021Quote: ... The autophosphorylation of hIRE1 KR was confirmed by western blot using a pSer/24 phospho-specific antibody (Genentech) and phosphorylated Pumilio proteins were detected using anti-mouse-V5 antibody (Invitrogen). In the case of kinase radioactive assays ...
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bioRxiv - Cell Biology 2021Quote: ... a 3-step approach was taken using a biotinylated secondary antibody and a final incubation step with a strepatividin-linked AlexaFluor antibody (Invitrogen). Slides were inserted into a 1 μg/ml solution of 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... The NFAp were incubated with primary antibodies for 1 h followed by secondary antibodies conjugated with either Alexa488 or Alexa594 (Invitrogen). SlowFade Gold Antifade Mountant (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... Incubation with blocking/permeabilization solution containing primary antibodies at 4 °C for overnight was followed by secondary antibody staining using suitable species-specific Alexa Fluor-coupled antibodies (Invitrogen) and flat-mounting in microscope glass slides with Fluoromount-G (0100-01 ...
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bioRxiv - Bioengineering 2022Quote: ... Additional labeling of C2C12 with MF-20 hybridoma mouse IgG2B primary antibody and Alexa Fluor 546 goat anti-mouse IgG2B secondary antibody (Invitrogen) was used to evaluate the cell differentiation into myotubes ...
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bioRxiv - Immunology 2022Quote: Antibody combinations starting at 30 µg/ml per antibody were serially diluted in Dulbecco’s Phosphate Buffered Saline (DPBS)(Gibco™) using half-log dilutions ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the anti-BiP mouse monoclonal antibody and the anti-mouse secondary antibody were obtained from Invitrogen (Grand Island, NE, USA). Dimethyl sulfoxide (DMSO ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and double immunostaining protocols were performed by sequential incubation with primary antibodies (Supplementary Table 16) followed by secondary Alexa Fluor antibodies (1:1000; Invitrogen). Images were collected using an Olympus (Tokyo ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-chlamydial MreB antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), or rabbit anti-six histidine tag (Genscript ...
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bioRxiv - Microbiology 2022Quote: ... Slides were incubated with primary antibodies followed by Alexa Fluor 488-conjugated goat anti-mouse secondary antibody or Alexa Fluor 594-conjugated goat anti-rabbit secondary antibody (diluted 1:400) (Invitrogen). The nuclei were stained with DAPI (10 μg/mL ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections of PVT were incubated in the same TH antibody solutions before transferred to a secondary Alexa-Fluor Plus 594 donkey anti-mouse antibody (1:3000; A21203, Invitrogen) for 2 hrs.
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bioRxiv - Cell Biology 2022Quote: ... three 5 μm-thick tissue sections separated by 300 μm were stained with guinea pig anti-insulin antibody (undiluted, Dako IR002; Alexa Fluor-488 secondary antibody, Invitrogen) and mouse anti-glucagon antibody (1:500 ...