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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for PKC beta 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and rabbit anti-GFP (polyclonal anti-GFP rabbit IgG, A11122 Invitrogen, 1:000 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with PBS and further incubated in the dark for 2 h with Alexa Fluor® 488-conjugated donkey anti-rabbit IgG secondary antibody (Thermo Scientific; 1:500). Then ...
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bioRxiv - Microbiology 2021Quote: ... Western blots were probed with α- CP or α-RRV E2 primary antibodies at a 1:5000 dilution in 2% milk in TBST followed by AlexaFluorTM750 goat α-rabbit secondary antibody (Invitrogen A21039, Carlsbad, CA) at a 1:20,000 dilution in 2% milk in TBST ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were washed three times in PBS-T and were incubated for 2 hr with the secondary antibodies Alexa Fluor® 488 or 546 Goat Anti-Mouse/Rat/Rabbit IgG (Life Technologies; 1:200) and then with DAPI (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-mouse and/or goat anti-rabbit AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500) secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... and then went through a 2 hr incubation with secondary donkey anti-rabbit antibody AlexaFluor 488 (dilution 1:500; #A-21206, Thermo Fisher Scientific, Waltham, MA) at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... the samples were incubated with 2 µg/mL Alexa Fluor 488 labelled Goat anti-Rabbit IgG secondary antibody (ThermoFisher Scientific, stock solution, 2mg/mL) prepared by mixing the stock solution in 0.1% Triton/DPBS at 1/100 and 0.2 mM Hoechst 33342 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... HeLa cells (human cervical ATCC CCL-2) and RK13 (Rabbit kidney cells, ATCC CCL37) were grown in Minimum Essential Medium (MEM; Gibco, Thermo Fischer, Lutterworth, UK) supplemented as described above for BSC-1 cells ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and incubated for 2 hours at RT with Alexa Fluor 488 donkey anti-rabbit (1:2000, Life Technologies-Invitrogen, Carlsbad, CA, USA) and Alexa Fluor 555 donkey antimouse (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... Cells labeled for Syt-7 were then incubated for 60 minutes with polyclonal cross-adsorbed un-conjugated F(ab’)2-Goat anti-Rabbit IgG (Invitrogen, ThermoFisher, Cat. # A24539, 1:300) diluted in TBS-0.2% BSA solution to bind the possibly remaining free primary antibody sites (anti-Syt-7 ...
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bioRxiv - Genetics 2020Quote: ... After washing and incubation with 1:2500 Alexa Fluor ® 568 F(ab’)2 fragment of goat anti-Rabbit IgG (H+L) (Thermo Scientific # A-21069) cells were stained with DAPI and mounted in VECTASHIELD (VectorLaboratories) ...
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bioRxiv - Neuroscience 2019Quote: ... followed by 2 hr incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen] made in PBS-T). Following three additional 10 min washes in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... slides were rinsed with 0.025% Triton X-100/1xTBS 2 × 5 min and incubated in goat anti-rabbit AlexaFlour488 secondary antibody (1:1000, Life Technologies, #A27034, Carlsbad, CA) at RT for at least 2 h followed by 4’,6-diamidino-2-phenylindol (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged and washed 2 times in cold flow buffer and resuspended in donkey-rabbit AF647 secondary antibody (Life Technologies, A31573, 1:1000) and DAPI (Biovision ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... sections were incubated with secondary antibodies for 2 h (anti-rabbit in goat Alexa-Fluor 488 nm, 1:500, Invitrogen Thermo Fisher Scientific, USA). For the second set of slices ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of 100 μM oligonucleotide bridge 2 and 2 units of T4 PNK (ThermoFisher) were added to 20 μl of the purified smDNA solution ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit antibodies were coupled to magnetic beads conjugated to anti-rabbit IgG (Dynabeads Sheep anti-rabbit IgG, Invitrogen, #11203D). The buffer containing 200 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Cells are differentiated for 4 days in embryonic body (EB) differentiation medium (DMEM/F12 [Hyclone], 20% FBS, NEAA [1×, GIBCO], GlutaMAX [1×, GIBCO], 0.1% beta-Mercaptoethanol [GIBCO]). For mixed embryonic body (EB ...
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bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778, Santa Cruz Biotechnology; GLUT2 1:1000, 720238 Thermo Fisher Scientific) for 16 h at 4C in blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems) in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Immunology 2022Quote: The binding capacity of the glycoengineered COVA1-18 (2C1) hIgG1 mAbs was tested by directly coating Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific) O/N at 4°C with 100 µL 1 µg/mL purified SARS-CoV-2 RBD-protein ...
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bioRxiv - Immunology 2022Quote: ... OEP light cages were applied to export individual cross-reactive mAb secreting B cells directly into Dynabeads mRNA DIRECT lysis buffer (Life Technologies, 61011) in 96-well plates ...
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bioRxiv - Microbiology 2022Quote: ... The internalization of the ACE2-HA protein and anti-hACE2 mAbs was then evaluated by staining with goat anti-mouse Alexa Fluor™ 594 (to detect the HA-tagged hACE2) and/or goat anti-human Alexa Fluor™ 488 antibodies (to detect the hACE2 mAbs) (ThermoFisher Scientific). Images were captured using a DeltaVision OMX SR imaging system (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... the unbound xfR5 mAb was washed off by dialysis using Float-A-Lyzer™ G2 Dialysis Devices (Thermo Fisher Scientific; NH, USA) in cold PBS supplemented with 10mM hydroxylamine to quench any non-reacted NHS groups present on the xfR5 mAb conjugated D+T NPs (xfR5-D+T NPs ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... and unlabeled anti-LTβR mAb and subsequently counterstained with streptavidin-Alexa 488 and goat-anti-rat-Alexa 488 (all Invitrogen, Breda, The Netherlands), respectively ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once before being stained with surface mAb (Table S1) and viability dye LIVE/DEAD Fixable Near-IR (Thermo-Fisher Scientific, USA) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... T-cells were incubated overnight at 4 °C with either mAb reactive to mouse phospho-CD247 (CD3 zeta) (Tyr111, Tyr123) (clone EM-55; Thermo Fisher Scientific) or mAb reactive to mouse ZAP70 phosphorylated at position 319 (clone 1503310 ...
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bioRxiv - Immunology 2023Quote: ... and next intracellularly stained with PE-conjugated anti-human phospho-Akt (Ser473) (D9E, CST) and anti-SIVmac251 Nef mAb (clone 17, Thermo Scientific/Pierce) manually conjugated with Alexa 488 or mouse IgG1 isotype control mAb (P3.6.2.8.1 ...
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bioRxiv - Microbiology 2023Quote: Monoclonal antibodies (mAbs) targeting β-GlcNAc-WTA (clone 4497) in different Ig isotypes and IgG subclasses were expressed in EXPI293F cells (Thermo Fisher) as previously described (29 ...
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bioRxiv - Immunology 2023Quote: ... anti-SIVmac251 Nef mAb (clone 17, epitope peptide corresponding to SIVmac239 Nef 71-80 not including the residue G63: Thermo Scientific/Pierce) manually conjugated with Alexa 488 or Alexa 647 ...
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bioRxiv - Immunology 2023Quote: ... they were resuspended in 0.5% BSA/PBS for prevention of experimental procedure-related loss and stained with mouse anti-SIVmac251 Nef mAb (clone 17, Thermo Scientific/Pierce) or mouse IgG1 isotype control mAb (P3.6.2.8.1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated in the dark for 30-45 min on ice with goat anti-mouse fluorochrome conjugated isotype specific mAbs (Life Technologies Corporation, Invitrogen, USA), washed twice and re-suspended in 2% BSA PBS ...
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bioRxiv - Immunology 2023Quote: ... were coated with 10 µg/mL of anti-LAIR-1 agonist mAb (clone Dx26) or 10 µg/mL of mouse isotype control IgG1 (eBioscience-Thermo Fisher Scientific) diluted in PBS overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 2 washes of 2× SSC (Ambion AM9765) for 5min ...
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bioRxiv - Immunology 2020Quote: ... 2-mercaptoethanol (2◻×◻10-5 M, ThermoFisher), penicillin (100◻IU◻per ml ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 0.05 mM 2-mercaptoethanol (2-ME; Gibco). Mouse melanoma cell line B16 (ATCC ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 mM GlutaMax supplemented with 2% B27 (Gibco) and 5% horse serum (Hyclone) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- D-glucose (2-NBDG) (Invitrogen N13195) was used as a probe for monitoring glucose uptake ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...