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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for 6 hydroxy 2 methylquinazolin 4 3H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... were blocked for 2 hr at 4 °C in lysis buffer containing 1 mg/ml yeast tRNA (Ambion) and washed twice with 1 ml lysis buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Immunology 2023Quote: ... Expression of GOI protein (Figure 2) was determined by SDS–PAGE using 4–12% gradient polyacrylamide gels (Invitrogen) and Coomassie staining and by Western blotting using antigen-specific sera.
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bioRxiv - Cell Biology 2022Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Neuroscience 2023Quote: ... NPC were passaged every 2-4 days by trypsinization in 0.05% trypsin EDTA (obtained from 0.25% Trypsin EDTA Gibco #25200056 ...
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bioRxiv - Physiology 2023Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was diluted 1:4 and 2 μL was used in a 20 μL qPCR Taqman assay (Invitrogen). Assay probes were used to detect cDNA from Nkx2.9 (Mm00435145_m1) ...
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bioRxiv - Physiology 2023Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30 °C ...
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bioRxiv - Immunology 2023Quote: ... we injected the mice intraperitoneally with 4 mg/ml of 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Waltham, MA) with the goal of labeling circulating monocytes to assess macrophage retention among groups ...
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bioRxiv - Immunology 2023Quote: ... or 2 µg H4K20me1 (Invitrogen) (were incubated overnight at 4 with rotation. The following day, Pierce beads (Invitrogen) were washed 3x in ChIP buffer ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were cultured from day 2 to day 4 in advanced RPMI 1640 (A-RPMI) (Gibco™ 12633012) supplemented with GlutaMax and 7.5 μM CHIR99021 ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm X 50 mm, 4 µm, Thermo Scientific) at 30°C ...
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bioRxiv - Neuroscience 2024Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Neuroscience 2024Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30°C ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were washed 4 times with 2× SSC and stained with 0.5 µg/ml DAPI (Thermo Scientific, 62248) in 2× SSC for 1 min ...
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bioRxiv - Cell Biology 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000015). Viral supernatant was harvested 48h after transfection and filtered through 0.45 µm cellulose acetate filters (Corning 431220) ...
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bioRxiv - Microbiology 2020Quote: ... 5-(and-6)-carboxyfluorescein (FAM) and 5-(and-6)-carboxytetramethylrhodamine (TAMRA) were obtained from Invitrogen™ ...
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bioRxiv - Plant Biology 2023Quote: ... 6 uL of 0.5 M EGTA (bioWORLD) and 6 uL of 0.5 M EDTA (Invitrogen) were added to quench the reaction at 65 °C for 10min ...
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bioRxiv - Cancer Biology 2022Quote: ... Five million C4-2 or two million SK-OV-3 cells in serum free media were mixed one to one with growth factor reduced Matrigel® (Invitrogen) in a total volume of 200 μl and injected in the hind flank using a 23- or 27-gauge needle ...
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bioRxiv - Microbiology 2020Quote: ... and 6 μl of the 2X Luna Universal One-Step Reaction Mix was subjected to one-step RT-qPCR using Applied Biosystems QuantStudio 3 (ThermoFisher Scientific), with the following cycling conditions ...
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bioRxiv - Microbiology 2021Quote: ... were performed with the Express one step RT-qPCR Universal kit (EXPRESS One-Step Superscript™ qRT-PCR Kit, universal Invitrogen) using 3.5µL of RNA and 6.5µL of RT qPCR mix and standard fast cycling parameters ...
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bioRxiv - Microbiology 2019Quote: ... The extracted RNA was reversely transcribed and amplified by a one-step RT-qPCR using a 48-well plate real-time PCR system Step-One (Applied Biosystems). The sets of primers and probes used for the VP1 and for the Elongation Factor −1 alpha (ELF1α ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We did One-step real-time RT-PCR (Step-One Plus real-time PCR thermocycler, Applied Biosystems, Foster City, CA, USA), using total nucleic acids extracted by Trizol® (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... and the concentration was measured using a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific ND-ONE-W). Amplification of 300 ng of DNA was performed using KOD Hot Start Master Mix (Sigma-Aldrich 71842) ...
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bioRxiv - Genetics 2023Quote: ... according to the manufacturer’s protocol and normalized to the correct concentration using the NanoDrop One Spectrophotometer (Thermo Scientific, ND-ONE-W). Samples were then submitted to the Center for Genome Sciences and Systems Biology (Washington University in St ...
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bioRxiv - Pathology 2024Quote: ... After one last freeze-thaw cycle the total protein content was measured on the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Pathology 2024Quote: ... Kidneys were divided longitudinally with one half stored in formalin for histological evaluation and one half placed in RNALater (Thermofisher Scientific). Sham animals underwent a midline laparotomy alone with no renal pedicle clamping ...
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bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
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bioRxiv - Cell Biology 2020Quote: 2 × 105 cells were transfected in a gelatin-coated 6-well plate in ES DMEM-2i using Lipofectamine 2000 (Fisher Scientific, 11668-027). Two pairs of gRNA were used for each gene (1 µg each ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Immunology 2022Quote: PECs were harvested from untreated wild-type B6 and Nr4a1-/- mice and allowed to adhere to plastic wells (6-well plates, 2 × 106 cells/well) for 1 hour in AIM V serum free medium (ThermoFisher Scientific, Waltham, MA). Non-adherent cells were washed off with PBS and the adherent cells were cultured for 3-hrs in complete DMEM + 10% FBS medium containing LPS (1 μg/ml in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were subsequently plated on islands of glial cells at a density of 1.25 × 104 cells/cm2 in 6-well plates in Neurobasal-A supplemented with 2% B27 and 0.2% penicillin/streptomycin (Invitrogen/ Thermo Fisher, Waltham, MA). Lentiviral particles encoding synaptoPAC were added 1–4 days after plating ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Germany) and 2 drops of glacial acetic acid (#3738.1; Roth, Karlsruhe, Germany) in 70% EtOH (#200-678-6, Fisher Scientific, Waltham, Massachusetts, USA) for 30 s and subsequently rinsed in aqua bidest ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells (wild-type or mS37 knock-out) were transfected in 6-well cell culture dish at 70% confluency with 2 μg plasmid using Lipofectamine 2000 (ThermoFisher scientific, Cat#11668027). Cells were collected after 72h ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... Magnetic bead removal and the evaluation of CAR expression on T cells by flow cytometry were performed on day 6 by staining with a goat anti-mouse F(ab’)2 antibody (Invitrogen, Carlsbad, CA, USA). CART cells were harvested and cryopreserved on day 8 for future experiments ...
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bioRxiv - Neuroscience 2024Quote: Collected rosettes were plated onto a polyhema-treated 6-cm Petri dish in NDMB medium containing NDM and 2% B27 w/o vitamin A (Gibco, Grand Island, NY), supplemented with 10ng/ml IGF-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... the input aliquot (100 µl) was mixed with equal volume of 2× protein sample buffer (2× NuPAGE LDS buffer [Life Technologies], 100 mM DTT, 4% [w/v] SDS) and the IP with 100μl 1 x protein sample buffer followed by incubation for 10 min at 75 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... and 5-6 pieces per well were cultured in a 6-well culture plate (Fisher Scientific). For up to two weeks tissue pieces were cultured in Advanced Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2021Quote: ... using 6 mL of cell suspension and 6 mL of Prolong Gold Antifade mountant (ThermoFisher Scientific) that were mixed on an object glass and then covered with a special STED cover glass ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Plasma IL-6 levels were measured by mouse IL-6 ELISA kit (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2023Quote: ... Serum was run through an interleukin-6 (IL-6) enzyme-linked immunoabsorbent assay (ELISA) kit (Invitrogen). Successful maternal immune activation in offspring of Poly(I:C ...
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bioRxiv - Developmental Biology 2022Quote: ... bTSCs were passaged every 6 days at a 1:6 split ratio using Accutase (Gibco, A1110501). Each well of bTSCs was dissociated by 1 mL Accutase for 5 mins at 38.5 °C ...
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bioRxiv - Immunology 2022Quote: ... IL-6 measurement was performed using IL-6 Mouse Uncoated ELISA Kit (88-7064-22; Invitrogen).
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bioRxiv - Molecular Biology 2019Quote: ... and (3) 110,000 × g for 2 hours at 4°C with a Sorvall Discovery SE ultracentrifuge (Thermo Fisher Scientific) with an AH-650 rotor (k factor ...