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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for 6 O TOLYL IMIDAZO 2 1 B THIAZOLE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 6 ml of stock solution was added to 294 mL of 0.1% acetic acid (Cat# UN2789, Fisher Scientific) in ddH2O and used within 10 min of preparation ...
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bioRxiv - Plant Biology 2020Quote: ... Organic acids were separated using an IonPac AS11-HC (2 mm, Thermo Scientific) column connected to an ICS-5000 system (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM L-Glutamine and 0.1 mM non-essential amino acids (ThermoFisher Scientific).
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bioRxiv - Immunology 2021Quote: Acid-washed 2 or 5μm boroscilicate glass beads (Duke Standards, Thermo Scientific, 9005) or 15mm glass coverslips/matteks were sequentially coated with poly-L-lysine ...
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bioRxiv - Biochemistry 2022Quote: ... Final protein concentration was assessed using 5,5-dithio-bis-(2-nitrobenzoic acid) (ThermoFisher) and a standard curve of glutathione (Aitken & Learmonth ...
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bioRxiv - Cancer Biology 2019Quote: ... Gels were run using 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Invitrogen NP000202) and transferred onto nitrocellulose transfer membrane using an XCell II Blot Module or iBlot Dry Blotting System (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... 2-propanol and formic acid were from Fisher Scientific (Fair Lawn, NJ, USA). Ammonium formate for mass spectrometry and toluene of analytical standard quality were from Sigma-Aldrich (Sigma Chemical Co. ...
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bioRxiv - Immunology 2023Quote: ... 2 mM Hepes and 0.1 mM non-essential amino acids (NEAA, all Gibco). 5×105 MC38OVA cells were subcutaneously (s.c. ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... and 2 mM Ethylene Diamine Tetra-acetic Acid (EDTA, Invitrogen, Cergy Pontoise, France). CD8+ and CD4+ T cells were purified by positive selecting using CD8 and CD4 microbeads ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 mM non-essential amino acids and 1 % penicillin/streptomycin (Invitrogen). NLRP3 mutated cell lines are H661 ...
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bioRxiv - Cell Biology 2019Quote: ... Na-pyruvate 1 mM and 1% nonessential amino acids (MEAM, GIBCO). T51B cells (rat epithelial cells lines ...
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bioRxiv - Immunology 2019Quote: ... 1% nonessential amino acids and 1% sodium pyruvate (all from Gibco).
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bioRxiv - Neuroscience 2021Quote: ... 1% penicillin-streptomycin and 1% MEM nonessential amino acids (ThermoFisher Scientific). SUMO1 deletion was carried out in striatal cells using CRISPR/Cas-9 tools obtained from Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... 1% glutamine and 1% non-essential amino acid (all Invitrogen, UK) in 5% CO2 at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... 1% non-essential amino acids and 1 mM sodium pyruvate (ThermoFisher) at 37°C with 5% CO2 and fed every 3-4 days ...
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bioRxiv - Cancer Biology 2021Quote: ... water with 0.2% formic acid and B: acetonitrile with 0.2% formic acid) were driven and controlled by a Dionex Ultimate 3000 RPLC nano system (Thermo Fisher Scientific). An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18 ...
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bioRxiv - Molecular Biology 2021Quote: ... was performed using double nucleic acid detection kit for respiratory syncytial virus (A/B) (Shenzhen shengkeyuan) with the ABI Q7 (Applied Biosystems). The reaction was conducted according to the manufacturer’s instructions with total volume of 20 μL.
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bioRxiv - Cancer Biology 2019Quote: ... and buffer B (80 % acetonitrile, 0.1 % formic acid) was used for peptide separation on an Easy-nLC 1200 (Thermo Fisher Scientific). This system was coupled via a nano electrospray ionization source to the quadrupole-based Q Exactive HF benchtop mass spectrometer 43 ...
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bioRxiv - Biochemistry 2021Quote: ... B: 80% acetonitrile/0.1% formic acid in water on a 50 cm Acclaim PepMap RSLC C18 column (Thermo Fisher Scientific #164942) operated by a Dionex ultimate 3000 RSLC nano pump with column heating at 50°C connected to an Orbitrap Fusion Lumos ...
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bioRxiv - Cancer Biology 2021Quote: ... B: 80% acetonitrile/0.1% formic acid in water on a 50 cm Acclaim PepMap RSLC C18 column (Thermo Fisher Scientific #164942) operated by a Dionex ultimate 3000 RSLC nano pump with column heating at 50°C connected to an Orbitrap Fusion Lumos ...
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bioRxiv - Biochemistry 2020Quote: ... buffer B: 80% (v/v) acetonitrile + 0.1% (v/v) formic acid) to a quadrupole-orbitrap Q Exactive Plus (Thermo Fisher Scientific) MS/MS via a nano-electrospray source (positive mode ...
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bioRxiv - Molecular Biology 2022Quote: ... buffer B: 80% (v/v) acetonitrile + 0.1% (v/v) formic acid) to a quadrupole-orbitrap Q Exactive Plus (Thermo Fisher Scientific) MS/MS via a nano-electrospray source (positive mode ...
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bioRxiv - Microbiology 2020Quote: Proteins were extracted from bioreactor cell pellets using glass bead beating (acid, washed, 0.1 mm diameter) in a suspension containing B-PER reagent (Thermo Scientific, Germany) and TEAB buffer (50 mM TEAB ...
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bioRxiv - Microbiology 2020Quote: ... water with 0.2% formic acid and B: acetonitrile with 0.2% formic acid) were driven and controlled by a Dionex Ultimate 3000 RPLC nano system (Thermo Fisher Scientific). An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18 ...
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bioRxiv - Microbiology 2021Quote: ... water with 0.2% formic acid and B: acetonitrile with 0.2% formic acid) were driven and controlled by a Dionex Ultimate 3000 RPLC nano system (Thermo Fisher Scientific). Gradient elution was performed at 300 nl min−1 ...
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bioRxiv - Biochemistry 2022Quote: ... water with 0.2% formic acid and B: acetonitrile with 0.2% formic acid) were driven and controlled by a Dionex Ultimate 3000 RPLC nano system (Thermo Fisher Scientific). Gradient elution was performed at 300 nL/min ...
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bioRxiv - Cell Biology 2023Quote: For flowcytometric quantification of GFP signal in induced 3D7-DiCre pFIO/pFIO+ strains 2.5 µl iRBCs were washed once with RT 0.9% NaCl solution (B. Braun GmbH) and stained with 5 µM SYTO 61 Red Fluorescent Nucleic Acid stain (Thermo Fisher Scientific) for 1 h at 37°C in 0.9% NaCl solution and washed once again afterwards at RT ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Mice were genotyped by PCR using forward primers 5’-ctgagcagagacccactgaaag-3’ and reverse primers 5’- ggatctggcttctgagtttgtgta-3’ and amplicons were ran in 6% TBE gels (Life Technologies, Carlsbad, CA).
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bioRxiv - Bioengineering 2023Quote: ... staining was used to visualise collagen distribution and orientation while Alcian blue (1% Alcian blue in 3% acetic acid, Thermo Fisher scientific, MA, USA) staining was used to qualitatively examine sGAG content ...
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bioRxiv - Microbiology 2021Quote: ... and mixed 1:1 with B-PER reagent (Thermo Fisher Scientific, Waltham, MA, USA). Cells were lysed by two rounds of sonification (Sonifier B-12 with microtip ...
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bioRxiv - Neuroscience 2019Quote: ... they were incubated with donkey anti-chicken rhodamine and donkey anti-rabbit Alexa 488 secondary antibodies for 1.5 hours and then with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen Life Technologies) (1:20 ...
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bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained for 30 min with 4’,6-diamidino-2-fenylindool (DAPI, 1:1000 in PBS, Thermo Fisher Scientific). After immunolabelling ...
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bioRxiv - Plant Biology 2021Quote: ... Tissues were counterstained with DAPI (4′,6-diamidino-2-phenylindole, 1 μg/ml in PBS) and mounted with ProLong Gold (ThermoFisher Scientific) using #1.5 coverslips (thickness ...
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bioRxiv - Microbiology 2020Quote: ... Light organs were then counterstained overnight with a 1:750 dilution of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) in 5x SSC-Tween before mounting on slides with Vectashield (Vector Laboratories ...
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bioRxiv - Genetics 2022Quote: ... Cells (1-2.5 x106/mL) were then cultured at 37 °C ex vivo for 2-6 hours in RPMI (Gibco, 21875-034) containing 10% FCS (IGC Technical Services ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All slides were then rinsed three times with PBS-T and twice with 1× PBS and coated with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images were acquired using an Olympus BX63 automated fluorescence microscope equipped with an Olympus DP74 digital camera and evaluated with cellSens Dimension software (Olympus).
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bioRxiv - Biochemistry 2020Quote: ... at 37°C for 20 min and stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:1000 in PBS, pH 7.4, Thermo Fisher Scientific) before imaging on a Nikon Eclipse Ti-E inverted microscope with a 100X lens (Apochromat 1.49 Oil 0.13-0.20 DIC N2 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected using 1-2 µg of pMX-GFP or pMX-53BP1 vectors and 6 µL Lipofectamine 2000 (Thermo Scientific). The media was changed after 3 hours of incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... The gonads were washed 3 times and then stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) before mounting with antifade solution (Invitrogen, P36980) on poly-L-lysine coated glass slides (Sigma Merck ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: ... followed by washing and addition of secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific, 62248) for 30 mins ...
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bioRxiv - Bioengineering 2023Quote: ... to each well we add 200 μL of 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat. No. D3571) solution in DI water ...
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bioRxiv - Systems Biology 2023Quote: ... the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... sections were counterstained with 1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, #D1306, Thermo Fisher Scientific, Waltham, MA, USA) then ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...