Labshake search
Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for 6 ISOPROPYLCHROMONE 2 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
-
bioRxiv - Cell Biology 2020Quote: 2 × 105 cells were transfected in a gelatin-coated 6-well plate in ES DMEM-2i using Lipofectamine 2000 (Fisher Scientific, 11668-027). Two pairs of gRNA were used for each gene (1 µg each ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
-
bioRxiv - Neuroscience 2021Quote: ... The nuclei of astrocytes and autaptic neurons were visualized by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI)-containing mounting medium (ProLong® Gold Antifade Reagent with DAPI, Thermo Fisher Scientific). Only neurons with one nucleus were analyzed when counting synapse numbers.
-
bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
-
bioRxiv - Immunology 2022Quote: PECs were harvested from untreated wild-type B6 and Nr4a1-/- mice and allowed to adhere to plastic wells (6-well plates, 2 × 106 cells/well) for 1 hour in AIM V serum free medium (ThermoFisher Scientific, Waltham, MA). Non-adherent cells were washed off with PBS and the adherent cells were cultured for 3-hrs in complete DMEM + 10% FBS medium containing LPS (1 μg/ml in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were again washed and incubated for 10 min in a solution of 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific). Cells were imaged using a Leica DM IRB epifluorescence microscope ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were subsequently plated on islands of glial cells at a density of 1.25 × 104 cells/cm2 in 6-well plates in Neurobasal-A supplemented with 2% B27 and 0.2% penicillin/streptomycin (Invitrogen/ Thermo Fisher, Waltham, MA). Lentiviral particles encoding synaptoPAC were added 1–4 days after plating ...
-
bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
-
bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... sections were rinsed three times for 10 min each and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific) as counterstaining.
-
bioRxiv - Immunology 2020Quote: ... A viability dye was included in all Duraclone panels (Live/dead fixable dead cell stain kit from ThermoFisher Scientific or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate from ThermoFisher Scientific (Courtabœuf, France)) ...
-
bioRxiv - Microbiology 2020Quote: ... Alexa-Fluor 594 labelled goat anti-rabbit IgG, ProLong Gold antifade reagent with DAPI (4’, 6-diamidino-2-phenylindole) (Life technologies-Invitrogen, USA), ATP Affinity kit (no ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells (wild-type or mS37 knock-out) were transfected in 6-well cell culture dish at 70% confluency with 2 μg plasmid using Lipofectamine 2000 (ThermoFisher scientific, Cat#11668027). Cells were collected after 72h ...
-
bioRxiv - Bioengineering 2023Quote: ... These slices were then fixed in 4’,6-diamidino-2-phenylindole (DAPI) mounting media (Fluoromount-G® with DAPI, Thermo Fisher Scientific, USA), and washed twice with PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... Slices were then stained with 4′,6-diamidino-2-phenylindole (DAPI) to stain cell nuclei and mounted with Immu-Mount mountant (Thermo Scientific, Cat# 9990402) onto microscope slides.
-
bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2022Quote: ... and blocked for 45 min in 2% bovine serum albumin (BSA) in PBS prior to staining with DAPI (4=,6-diamidino-2-phenylindole) and mounted in Prolong Diamond antifade (Molecular Probes, Life Technologies). Images were captured using a Nikon Eclipse Ti confocal microscope (Nikon Instruments ...
-
bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
-
bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
-
bioRxiv - Neuroscience 2024Quote: Collected rosettes were plated onto a polyhema-treated 6-cm Petri dish in NDMB medium containing NDM and 2% B27 w/o vitamin A (Gibco, Grand Island, NY), supplemented with 10ng/ml IGF-1 ...
-
bioRxiv - Immunology 2024Quote: ... Magnetic bead removal and the evaluation of CAR expression on T cells by flow cytometry were performed on day 6 by staining with a goat anti-mouse F(ab’)2 antibody (Invitrogen, Carlsbad, CA, USA). CART cells were harvested and cryopreserved on day 8 for future experiments ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cells were rinsed three times with 1X PBS and coated with ProLong Gold antifade mounting medium with DAPI (4’,6-diamidino-2-phenylindole, 100 ng/ml) (Life Technologies, P-36931) on slides ...
-
bioRxiv - Immunology 2020Quote: For acid induced unfolding experiments the hydrophobic dye BIS-ANS (4,4’-dianilino1,1’-binaphthyl-5,5’-disulfonic acid, Invitrogen) was used to monitor protein unfolding by fluorescence spectroscopy with an excitation wavelength of 390 nm ...
-
bioRxiv - Immunology 2023Quote: ... and 1X non-essential amino acids (MEM non-essential amino acids solution 100X, Gibco, Waltham, Massachusetts, USA). Cell cultures were maintained at 37 °C and 5% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were concentrated onto the trap column at 5 μL/min for 5 minutes with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific) at 300 nL/minute via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were concentrated onto the trap column at 5 μL/min for 5 minutes with Buffer A (0.1% formic acid, 2% DMSO) and infused into a Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific) at 300 nl/minute via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were concentrated onto the trap column at 5 μL/min for 5 minutes with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific) at 300 nl/minute via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were concentrated onto the trap column at 5 μL/min for 5 minutes with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific) at 300 nl/minute via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... Each fraction was reconstituted in solvent A (0.1% formic acid) and loaded onto trap column (75 μm x 2 cm) Thermo Scientific™ Acclaim™ PepMap™ 100 C18 (#164535; Thermo Scientific) (3 μm particle size ...
-
bioRxiv - Cell Biology 2021Quote: ... Digestion was stopped by the addition of 2% (v/v) formic acid before peptides were cleaned using Pierce C18 spin columns (Thermo Fisher Scientific, cat#89870) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... Peptides were reconstituted in 0.1% trifluoroacetic acid (TFA) and 2% acetonitrile (ACN) and loaded onto a PepMap C18 5 µm 1 cm trapping cartridge (Thermo-Fisher Scientific, Waltham, MA, USA) at 12 µL/min for 6 min before switching the pre-column in line with the analytical column (nanoEase M/Z Peptide BEH C18 Column ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were trypsinized at 80%–90% confluence with 0.15% trypsin / ethylene diamine tetraacetic acid (EDTA) (0.5% Gibco trypsin EDTA diluted 1:2 in PBS). Cells were immediately removed from the plate with 10 ml ice-cold DMEM containing 10% FBS ...
-
bioRxiv - Immunology 2024Quote: ... PCR products were loaded on a 2% agarose gel in 1X TAE buffer containing 0.01% MilliporeSigma™ GelRed™ Nucleic Acid Stain (Fisher Scientific, catalog # SCT123) with electrophoresis performed at 200V for 30 minutes for the 3’ loxP sequence reaction and 40 minutes of the 5’ loxP sequence reaction ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 5-6 pieces per well were cultured in a 6-well culture plate (Fisher Scientific). For up to two weeks tissue pieces were cultured in Advanced Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Microbiology 2021Quote: ... using 6 mL of cell suspension and 6 mL of Prolong Gold Antifade mountant (ThermoFisher Scientific) that were mixed on an object glass and then covered with a special STED cover glass ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Plasma IL-6 levels were measured by mouse IL-6 ELISA kit (ThermoFisher Scientific, Waltham, MA).
-
bioRxiv - Immunology 2022Quote: ... IL-6 measurement was performed using IL-6 Mouse Uncoated ELISA Kit (88-7064-22; Invitrogen).
-
bioRxiv - Neuroscience 2023Quote: ... Serum was run through an interleukin-6 (IL-6) enzyme-linked immunoabsorbent assay (ELISA) kit (Invitrogen). Successful maternal immune activation in offspring of Poly(I:C ...
-
bioRxiv - Developmental Biology 2022Quote: ... bTSCs were passaged every 6 days at a 1:6 split ratio using Accutase (Gibco, A1110501). Each well of bTSCs was dissociated by 1 mL Accutase for 5 mins at 38.5 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... 6 ml basic F12 media (GIBCO) was added and 500 μl of FBS (Hyclone ...
-
bioRxiv - Developmental Biology 2019Quote: ... and dispase (6 mg/mL, Gibco) for 1 h at 4 °C ...
-
bioRxiv - Neuroscience 2019Quote: ... IL-6 (20 ng/mL, ThermoFisher). To generate human induced pluripotent stem cells (iPSCs) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Then 6 μl Lipofectamine RNAiMAX (Invitrogen) was added and supplemented to a total volume of 250 μl with optiMEM ...
-
bioRxiv - Immunology 2021Quote: ... using QuantStudio 6 platform (ThermoFisher Scientific). Ct values were normalized to the Ct values for Ecad (Btln1,4,6,Il15 ...
-
bioRxiv - Genomics 2021Quote: ... Empty 6% TBE gel (EC62655BOX, Invitrogen) was run at 100V in 0.5x UltraPure TBE Buffer (15581-044 ...
-
bioRxiv - Immunology 2022Quote: ... 2DG (Thermo Fisher, 6 g/L) was dissolved in drinking water and was provided to mice ad libitum ...
-
bioRxiv - Immunology 2020Quote: ... and 6 μg/ml CpG (Invitrogen) in complete RPMI in an incubator at 37°C/5% CO2 for 5 days ...
-
bioRxiv - Microbiology 2021Quote: ... 6 µl dNTPs 2.5 mM (Invitrogen), and 2 U of AMV RT (Life Sciences ...