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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Recombinant Mouse Cd274 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... redundant anti-His antibody was washed away and 15 μL chemiluminescence substrate (Thermo Scientific) was added onto each well to generate chemiluminescence signal ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco™ Cat. #10438026) and 1% antibiotic/antimycotic (Gibco™) ...
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bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 30 minutes on ice with His-Pur Ni-NTA resin (Thermo Scientific), packed to a height of approximately 5 cm in polypropylene columns ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
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bioRxiv - Molecular Biology 2022Quote: ... Junction PCR was performed with Extensor Hi-Fidelity PCR Master Mix (Thermo Fisher Scientific) in a 20 μL reaction using 500 nM of target-specific primers and the following cycling conditions ...
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bioRxiv - Neuroscience 2023Quote: ... and subcloned into the pcDNA™3.1/myc-His(−)A vector (Thermo Fisher Scientific). We accidentally obtained the pcDNA™3.1/myc-His(−)A-(G4C2)9 vector at this step ...
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bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
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bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
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bioRxiv - Cell Biology 2024Quote: ... and His-p115 were affinity purified using Ni-NTA HisPur beads (Thermo Fisher Scientific) from crude E ...
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bioRxiv - Evolutionary Biology 2021Quote: ... T0M20 antibodies (polyclonal, cat number PA5-52843) and Alexa 568-tagged secondary antibodies were from ThermoFisher. The imaging was performed using Nikon SP-1 microscope and analyzed using FIJI (Schindelin et al. ...
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bioRxiv - Cell Biology 2019Quote: Creation of the 3xmEGFP tagged RabE12 construct occurred by a two-element LR Gateway reaction (ThermoFisher) of entry clones pENT-L1-3xmEGFP-L5r and pENT-L5-RabE12-L2 ...
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bioRxiv - Neuroscience 2020Quote: ... N-terminally HA-tagged AMPAR constructs were expressed from the doxycycline inducible pcDNA4/TO vector (Invitrogen Cat ...
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bioRxiv - Biochemistry 2021Quote: Immunoprecipitation of HA tagged GAPDH (TDH3-HA) was performed using Pierce anti-HA beads (Thermo Fisher). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... 200 μl and the tagged Aβ(1-40) is incubated with Ulp1 SUMO-protease (either Invitrogen or produced recombinantly (Malakhov ...
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bioRxiv - Biophysics 2021Quote: ... they were transfected with EGFP tagged wt LA/A350P DNA along with Lipofectamine 2000 (Invitrogen, USA) in a ratio 1:1.5 ...
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bioRxiv - Bioengineering 2022Quote: MCF-7 Cells were fluorescently tagged with CellTracker™ Green CMTPX dye (Invitrogen™, Waltham, MA). A stock solution of 10 mM was prepared according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: 3T3-L1 cells expressing GFP-tagged human CIDEC were incubated with MitoTracker (#M7512; Thermo Fisher Scientific) before fixation ...
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bioRxiv - Neuroscience 2020Quote: ... In some cases 200-400 nl of fluorescent-tagged material (20% solution of FluoroEmerald Molecular Probes, Eugene ...
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bioRxiv - Microbiology 2020Quote: The Avi-tagged BG505 gp140.664.R1 trimer probe was transiently expressed in HEK293F cells (Thermo Fisher) (67) ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
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bioRxiv - Immunology 2020Quote: ... The resultant FLAG-tagged STAT3 constructs were transfected into 293T using lipofectamine 3000 (Thermo Fisher Scientific). 48 hours after transfection cells were lysed (50mM Tris ...
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bioRxiv - Genetics 2019Quote: ... Proteins tagged with the V5 epitope (Hxk2, Reg1) were detected with the Anti-V5 probe (Invitrogen) diluted 1:1,000 ...
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bioRxiv - Plant Biology 2022Quote: ... GST and GST-tagged SAMS were incubated with 40 μl GST magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C and then these beads were washed three times with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... we used PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Genetics 2023Quote: ... Flag-tagged proteins were immunoprecipitated with anti-Flag magnetic agarose (Pierce Anti-DYKDDDDK Magnetic Agarose, ThermoFisher), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and stained with Alexa Fluor 555-tagged donkey-α-goat secondary antibody (Molecular Probes, 1:1000) for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... Hexa-histidine tagged mTHSD7A fragments were purified under native conditions using Ni-NTA resin (Thermo Scientific) applying the batch method according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Rab7a and LAMP1 wt and mutant molecules tagged with EGFP using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... WRN Halo-tagged HCT-116 cells were seeded in FluoroBrite DMEM (Thermo Fisher, cat. no. 1896701) supplemented with 10% FBS (Corning) ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged gDNA was enriched using Dynabeads™ MyOne™ Streptavidin C1 (Thermo Fisher Scientific, Cat. 65001). The beads were allowed to room temperature while being resuspended on a HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... grown in 12-well plates in DMEM supplemented with 10% FCS (Gibco-BRL) and glutamine ...
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bioRxiv - Microbiology 2022Quote: ... Nb fused with Fc was purified using Protein G (cat.# 20399, Thermo Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Opti-MEM and fetal calf serum (FCS) were from GIBCO (Waltham, MA, USA). All cell culture ware was purchased from BD Falcon (Corning ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific), 50µM β-mercaptoethanol (Sigma) ...
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bioRxiv - Immunology 2019Quote: ... Cell pellets were resuspended in FACS buffer (5% FCS in PBS, Life Technologies). After adding Fcγ receptor block (1:100 dilution ...
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bioRxiv - Genomics 2019Quote: A375 cells were grown in DMEM + 10% FCS + Penicillin/streptomycin (Gibco, Carlsbad, MA) and were treated with 10,000 J/m2 UVB 310 nm (7 mins @ 25 J/m2/s ...
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bioRxiv - Genetics 2020Quote: ... with a final quench in PBS + 2% fetal calf serum (FCS, Invitrogen 26140079). Cells were permeabilized for 5 minutes with saponin buffer (PBS + 0.2% Saponin (Sigma S7900 ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% PenStrep (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% PenStrep (Thermo Fisher Scientific). Imaging was performed in a heating chamber preheated to 37 °C and placed on an inverted microscope (IX83 from Olympus ...
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bioRxiv - Systems Biology 2020Quote: ... PHHs were cultivated in Williams medium E (Biochrom) supplemented with 10% FCS (Gibco), 100 nM dexamethasone ...
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bioRxiv - Molecular Biology 2021Quote: ... All media were supplemented with 10% fetal bovine serum (FCS, Biochrom or GIBCO), 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% FCS and 1% Non-essential amino acids (Thermo Fisher #11140050)) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 15% fetal calf serum (FCS) and 2 mM Gln (ThermoFisher Scientific) at 37°C in 1% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... washed in sterile Schneider’s medium containing 20% fetal calf serum (Schneider’s/FCS; Gibco), and eventually homogenized with micro-pestles in 1.5 centrifuge tubes containing 21 embryos per 100 μl dispersion medium (Prokop et al. ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% heat-inactivated FCS (Interpath services) and 2mM L-glutamine (Invitrogen) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 2.5% FCS and 100 IU/ml penicillin and 100 mg/ml streptomycin (Gibco). CD4+ T cells were isolated from PBMC using CD4+ T cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Colorimetric changes were measured using an ELISA microplate reader (MULTISKAN FC, Thermo Scientific) at 450 nm ...