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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Recombinant Mouse Ager His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Stable cell lines expressing low levels of HA-tagged Gli2 and Gli3 variants were generated using the Flp-In system according to the manufacturer’s recommendations (Life Technologies) (22) ...
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bioRxiv - Plant Biology 2021Quote: Expression vectors for HA-tagged PpCSLDs under control of their native promoters were constructed using Gateway Multisite Pro cloning (Invitrogen). The CSLD2 and CSLD6 coding sequences were amplified from cDNA clones pdp04669 and pdp21814 (RIKEN BioResource Center ...
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bioRxiv - Molecular Biology 2022Quote: NiV prefusion glycoprotein and avi-tagged probes were expressed by transfection in 293 Freestyle (293F) cells (Thermo Fisher Scientific, MA) using Turbo293 transfection reagent (SPEED BioSystem ...
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bioRxiv - Cell Biology 2022Quote: Histidine-tagged Mindin was purified from conditioned media collected from CHO-Mindin cells using Ni-NTA beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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Peptide Arrays of Three Collections of Human Sera from Patients Infected with Mosquito-Borne VirusesbioRxiv - Microbiology 2019Quote: ... The arrays were then incubated for 1 h with goat anti-human IgG tagged with Alexa Fluor® 488 (Invitrogen) as secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... The resulting TGD057 HA-tagged DNA fragment was then cloned into the pTKO-att parasite expression vector (92) by Gateway Recombination Cloning Technology (Invitrogen). The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression constructs coding for His6/SUMO tagged fusion proteins of rat SHANK3 were generated in pET-SUMO (Thermo Scientific) as described 14 ...
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bioRxiv - Biochemistry 2020Quote: ... the clarified lysates from His-tagged or dual tagged constructs were loaded onto an Nickel-nitrilotriacetic acid (Ni2+-NTA; 1.6 mL suspended beads/L growth) column (Thermo Scientific) equilibrated with 10 column volumes (CVs ...
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bioRxiv - Molecular Biology 2019Quote: ... 6X N-terminally Myc-tagged cDNAs encoding Ku70 or Ku80 WT and mutants were cloned into pcDNA/FRT/TO (Invitrogen) and then transfected along with Flp recombinase expressing pOG44 plasmid using Lipofectamine transfection reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... For Protein A-tagged strains, immunoprecipitation was carried out with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) as described 57 ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections were washed thoroughly and then incubated with appropriate secondary antibodies tagged with A lexaFluor-488 or -594 (1:500; Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Stably transfected HEK293 cells expressing N-terminally FLAG-tagged MOR or KOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2021Quote: ... concentrated eluates issued from immunopurification of endogenous and RACK1-HA-tagged protein are separated on 10% Tris-glycine SDS-PAGE gels (Invitrogen), and stained with Imperial Protein Stain (Thermo Fisher) ...
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bioRxiv - Biophysics 2020Quote: ... HA or GFP-tagged DISP1 variants (anti-HA rabbit polyclonal, 1:1000 or anti-GFP rabbit polyclonal, 1:2000, Invitrogen), and β-actin (anti-β-actin rabbit polyclonal ...
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bioRxiv - Neuroscience 2022Quote: ... or isolated human synaptoneurosomes from AD brains (Tai et al., 2014; Tzioras et al., 2019) tagged with pHrodo Red succinimidyl ester (1:20, ThermoFisher) by media change ...
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bioRxiv - Genomics 2022Quote: A total of 6,114 animals from all year-classes evaluated in the sea lice challenges were pit-tagged and genotyped using custom ThermoFisher (Affymetrix) SNP arrays developed exclusively for AquaGen’s Atlantic salmon populations ...
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bioRxiv - Neuroscience 2022Quote: ... we incubated them in secondary antibody solution (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated in Alexa Fluor 647-tagged donkey antirabbit IgG (1:500, Thermo Fisher, catalog # A-31573, RRID: AB_2536183) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no.A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Neuroscience 2024Quote: ... blocked with 10 % NDS-PB-TX and incubated in the dark in secondary fluorescently tagged anti-mouse antibody for either TH or GAD1 (1:200, MAB5406, EMD Milipore)) and fluorescently tagged anti-rabbit antibody for tracer (1:200, AlexaFluor, Invitrogen), overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: For expression analysis of truncated WDR47 constructs HEK cells were transfected with different HA tagged Wdr47 constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... human protein database with common lab contaminants and the sequence of the tagged bait proteins added using SEQUEST within Proteome Discoverer 1.4 (Thermo Fisher). The search parameters were as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were transfected with a GFP-tagged plasmid vector expressing spCas9 and gRNA using Lipofectamine 3000 Transfection Reagent following manufacturer’s protocol (ThermoFisher Scientific). Cells were then sorted based on GFP expression after 48 hours from the transfection.
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bioRxiv - Cell Biology 2024Quote: ... We generated a strain in which Dnm1 is tagged with mcherry and Mmm1 with GFP (MCY2199) in which mitochondria were labelled with Mitotracker (#M22426, Invitrogen). This setup allowed following and quantifying the presence of Dnm1-mCherry and Mmm1-GFP at sites of mitochondrial fission ...
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bioRxiv - Cell Biology 2024Quote: ... and transfected with a total of 2 μg C-terminal EGFP tagged M2 tail using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer protocol after washing with 200 μL PBS and refreshing with 100 μL DMEM (ScienCell ...
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bioRxiv - Cell Biology 2024Quote: ... the GFP-tagged proteins were immunoprecipitated by incubating the extracts with 15 μl of anti-HA magnetic beads (Thermo Scientific) for 2 h at 4°C with gentle shaking ...
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bioRxiv - Biochemistry 2024Quote: ... or expressing EGFP-tagged HRas V12/ HRas V12-C181S/ HRas V12-C184S were lysed in TRIZol at the end of the experiment (Invitrogen). RNA was isolated from the lysate by the chloroform-isopropanol method using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The Fab from the CD4i antibody 17b37 was expressed by transient transfection using expression vectors encoding the LC and a C-terminally tagged HC portion of the Fab using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with goat anti-rat IgG (H+L) secondary antibody tagged with Alexa Fluor 488 (Invitrogen, A-21470). Tissue sections were washed and blocked with 1:100 mouse Fc block in 5% goat serum ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged proteins were then eluted with room temperature Elution Buffer with 2.5 mg/mL 3XFLAG Peptide (Thermo Fisher # A36806) for 15 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Y-A or YIY-AAA) and FLAG-tagged CDK-1 constructs were expressed in FreeStyle 293-F cells (ThermoFisher Scientific). Cells were transfected using FreeStyle MAX Reagent and OptiPRO SFM according to the manufacturer’s guidelines (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed three times for 10 min in 1X PBS and incubated for 4 h at 4°C with the fluorescently tagged secondary antibodies (Invitrogen; AlexaFluor 647 goat anti-mouse and AlexaFluor 488 donkey anti-rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... were transfected with expression vectors for 3xFLAG-tagged wt eIF3g or its mutated forms using Expifectamine 293 according to the manufacturer’s protocol (Thermo Scientific). Cells expressing 3xFLAG-tagged eIF3g (200 ml suspension ...
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bioRxiv - Biochemistry 2022Quote: The MS/MS spectra were searched against the NCBI Mycobacterium smegmatis database with common lab contaminants and the sequence of the tagged bait proteins were added using SEQUEST within Proteome Discoverer 1.4 (Thermo Fisher). The search parameters were as follows ...
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bioRxiv - Cell Biology 2023Quote: Exon 1 carrying C-terminally tagged HttQ25-EmGFP and HttQ46-EmGFP were generated in the mammalian expression vector pTREX (Invitrogen). VCP (wt)-EGFP (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... Myc or FLAG-tagged procaspase-9 plasmid was prepared by cloning the procaspase-9 gene into a pcDNA-based vector (Invitrogen). Procaspase-9 with three mutations (Asp315Ala ...
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bioRxiv - Cell Biology 2022Quote: ... and 50 ng Myc-tagged GFP constructs were forward transfected into HFT cells seeded in a 6-well plate using Lipofectamine 3000 (Invitrogen). The cells were harvested 48 or 72 hours post transfection ...
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bioRxiv - Biochemistry 2023Quote: ... 155-238) were fused to the C-termini of tagged receptors and cloned into the NheI-XhoI sites of pCEP4 (Invitrogen). Plasmids for HIV-1 MN gp160 and BaL gp160 (# 100919 ...
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bioRxiv - Cell Biology 2023Quote: Lenti-X 293T cells were transfected with pCDNA3.1 plasmids expressing HA-tagged YWHA isoforms using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol and cultured for 48 h before lysis in 2% SDS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... or expressing EGFP-tagged HRas V12/ HRas V12-C181S/ HRas V12-C184S were lysed in TRIZol at the end of the experiment (Invitrogen). RNA was isolated from the lysate by the chloroform-isopropanol method using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells stably expressing N-terminally FLAG-tagged DOR or MOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 2.5 μg of GST-tagged RIT1 plasmids or GFP control plasmid using Lipofectamine 3000 Reagent (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and RAF39 driven by the cauliflower mosaic virus (CaMV) 35S promoter and C-terminally tagged with green fluorescent protein (GFP) was achieved using Gateway LR Clonase II (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells stably expressing N-terminally signal sequence FLAG (ssf)-tagged µOR (HEK293-µOR) were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Cell Biology 2023Quote: ... The ESCO1 cDNAs were cloned into a pcDNA5/FRT-based C-terminally Flag- or GFP-tagged vector and co-transfected with a plasmid expressing the FLP recombinase (pOG44, Invitrogen) using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Flp-In T-Rex-293 cells were co-transfected with the plasmid coding for the tagged LTP and the pOG44 plasmid encoding the Flp recombinase (Invitrogen). Positive clones were selected by adding 100 µg/ml hygromicin B and 15 µg/ml blasticidin on the day after transfection ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: Rescue experiments were conducted in LRBA-KO HEK293T and HaCat cell lines transfected with 2 μg of WT LRBA plasmid tagged with myc (Origen) using Lipofectamine 2000 (Invitrogen). These reconstituted cells lines are called Myc-LRBA in this manuscript ...