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Citations for Thermo Fisher :
1751 - 1800 of 7250 citations for Recombinant Human SRPK1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... RNA extracts were stabilized by adding the RNAseOUT recombinant ribonuclease inhibitor (Thermo Fisher Scientific), before freezing at -80°C until ready for further analysis.
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bioRxiv - Plant Biology 2023Quote: ... RNA extracts were stabilized by adding the RNAseOUT recombinant ribonuclease inhibitor (Thermo Fisher Scientific), before freezing at −80°C until further analysis.
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bioRxiv - Biophysics 2023Quote: Recombinant baculovirus was generated by transfecting Sf9 cells with bacmid using Cellfectin II (ThermoFisher). Baculoviruses were harvested from Sf9 cell media by filtering through a 0.22 µm filter and amplified three times before using for cell transduction ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was resolved by NuPAGE™ 4-12% Bis-Tris Gel (Invitrogen; NP0322BOX) to determine identity and purity ...
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bioRxiv - Biochemistry 2023Quote: The following recombinant proteins were purchased from commercial sources: alkaline phosphatase (Thermo Scientific #EF0651), Antarctic phosphatase (New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: ... the epidermis was separated from the dermis and incubated in TrypLETM recombinant enzyme (ThermoFisher) for 1h and subsequently dissociated mechanically with a pipette tip ...
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bioRxiv - Immunology 2023Quote: ... and recombinant Cas9 protein via electroporation using the Neon transfection system (Thermo Fisher Scientific). The following day ...
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bioRxiv - Immunology 2023Quote: ... The recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculoviruses were generated by transfecting purified bacmid DNAs into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific) and amylose resin (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... Recombinant anti-GFP nanobody was conjugated to epoxy-activated magnetic Dynabeads M270 (Life Technologies) using 10 μg recombinant protein/1 mg Dynabeads ...
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bioRxiv - Microbiology 2023Quote: ... and STRN3 were carried out utilising immobilised recombinant protein A/G resin (Thermo Scientific). Cell pellets were harvested and processed as described in the EGFP co-immunoprecipitation section ...
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bioRxiv - Neuroscience 2022Quote: ... An injection of Cholera Toxin Subunit B (recombinant) conjugated to Alexa Fluor 647 (ThermoFisher) was made in the striatum of the same hemisphere (+1.2mm AP ...
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bioRxiv - Immunology 2023Quote: ... Recombinant antigen was immobilized on N-hydroxysuccinimide (NHS)–activated agarose resins (Thermo Fisher, 26197) by overnight rotation at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained with anti-CD64 (CD64 Recombinant Rabbit Monoclonal Antibody, Thermofisher, # MA5-29706) at a dilution of 1:400 in 1% BSA in PBS and incubated at 4°C overnight in the dark overnight ...
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bioRxiv - Immunology 2023Quote: ... recombinant bacmid DNA was generated using the Bac-to-Bac system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Recombinant RBD (Arg319 – Phe541) of the same strain was obtained from Invitrogen (# RP 87678) and used as an immunogen without conjugation with KLH and used for screening assays ...
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bioRxiv - Neuroscience 2024Quote: ... recombinant polyclonal goat anti-mouse coupled to Alexa Fluor 488 (Thermo Fisher, A-28175), and polyclonal goat anti-mouse coupled to Alexa Fluor 568 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Cytochalasin D (C8273, Neta) along with recombinant Mouse TNF-α (# RMTNFAI, Thermo Fisher Scientific) and human TNF-α (16769S ...
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bioRxiv - Biophysics 2021Quote: ... Control Drosophila melanogaster biotin-tagged kinesin-1 motors were labeled with streptavidin-conjugated Qdot 655 (Life Technologies, Carlsbad, CA) at a 1:4 motor:Qdot ratio as previously described [16 ...
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bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
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bioRxiv - Cell Biology 2020Quote: ... The pDONR201 clones were transferred into GFP or SFB tagged DEST vectors using the Gateway LR cloning system (Invitrogen). KDM5AΔPID deletion mutant was generated by PCR in pDONR201 vector following standard cloning method and then subcloned into Gateway destination vector derived from pcDNA5/FRT/TO plasmids containing the NLS and SFB- or GFP-tagged in N-terminal ...
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bioRxiv - Cell Biology 2020Quote: ... GFP or luciferase tagged versions of the proteins were generated using the Gateway recombination cloning procedure (Invitrogen, Carlsbad, CA) and custom-made vectors.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells grown on 100-mm dishes were cotransfected with Flag-tagged ASK1_KR and PKA or empty vector using Lipofectamine 2000 (Invitrogen). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (Tf) and Epidermal Growth Factor (EGF) tagged with Alexa Fluor 488 was obtained from Invitrogen (T13342 and E13345). Dako fluorescent mounting medium from Agilent (S3023 ...
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bioRxiv - Genomics 2020Quote: ... 20.25 μg 3×Flag-tagged dCas9 plasmid and 20.25 μg sgRNA plasmid were transfected into cells using Lipofectamine 3000 (Invitrogen). Two biological replicates were performed for each condition ...
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bioRxiv - Cancer Biology 2021Quote: Control MCF-10A cells or cells expressing HA-tagged PEAK3 were cultured in -EGF medium (DMEM/F-12 (Gibco) supplemented with 0.5◻μg/ml hydrocortisone (Sigma) ...
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bioRxiv - Biophysics 2021Quote: Full-length HA-tagged p75NTR and full-length Myc-tag TRADD were expressed from a pcDNA3 vector backbone (Invitrogen). The HA-p75E345A ...
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bioRxiv - Biochemistry 2021Quote: N-terminal GST-tagged MBNL1 (amino acids 1-260)59 was recombinantly expressed using BL21 Star (DE3) cells (Invitrogen) and protein expression induced using 0.5 mM IPTG at an OD600 = 0.6-0.7 for 2 hours at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... and the tagged SΔTM was purified by affinity chromatography using Ni-NTA agarose beads (Invitrogen™, Waltham, MA, USA) and size exclusion chromatography as described above.
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bioRxiv - Microbiology 2021Quote: ... bacteria were tagged by Gfp or stained by cell membrane stain FM4-64 (10 µM final concentration, Molecular Probes) for 2 mins in the dark (without flow) ...
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bioRxiv - Cell Biology 2021Quote: ... Stable cell lines expressing BioID tagged genes of interest were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5/FRT/TO construct and Flipase expressing pOG44 plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids for expressing tagged proteins in S2 cells were generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF) ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Cells at 40% confluence were transfected with 1 ug of GFP-tagged LRRK2 plasmid using Lipofectamine 2000 (Invitrogen, 11668019) as described for 12-15 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified A-C/EBP (30 - 40 μg) was tagged with Alexa Fluor 488 according to manufacturer’s protocol (Thermo fisher). Time lag expression of A-C/EBP protein was observed using EGFP tagged A-C/EBP plasmid (pCMV-A-C/EBP-EGFP ...
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bioRxiv - Immunology 2019Quote: ... The labeled cells were counter-stained with a fluorophore-tagged secondary goat anti-rabbit IgG (H+L) antibody (Invitrogen), before acquisition on a LSR Fortessa (BD Biosciences) ...
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bioRxiv - Biophysics 2020Quote: ... The C-terminally SBP-tagged DISP1-A or DISP1-A-NNN was captured using Streptavidin-agarose affinity resin (ThermoFisher). The eluted protein was concentrated by an Amicon Ultra filter (100 kDa cutoff ...
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bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... captured dsRNA was detected by incubating with sulfo-tagged rat anti-mouse IgM antibody (Thermo Fisher Scientific, Waltham, MA) for 1h before washing and adding MSD GOLD Read Buffer A (Meso Scale ...
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bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were subsequently washed with TSB and incubated with WGA tagged with Alexa Fluor 488 (WGA-488, Invitrogen) at a final concentration of 2 μg/ml for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Transgenic S2 cells carrying the SIN3 187 transgene tagged with an HA epitope were grown in medium containing 0.1 mg/ml penicillin and streptomycin (Gibco). For treatment with CuSO4 ...
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bioRxiv - Plant Biology 2023Quote: ... Epitope-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).