Labshake search
Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for PD 1 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... and 5 mg/mL human insulin (ThermoFisher) freshly supplemented with 100 ng/mL IL-34 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Primary human hepatocytes (Gibco/Thermo Fisher Scientific) were thawed into a 24-well Collagen I coated plate (Gibco/Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... biotin anti-human IgA Fab (Invitrogen A24462) was added at a dilution of 1:100,000 for 2 hours at 4 C to detect human IgA ...
-
bioRxiv - Cell Biology 2019Quote: ... The samples were then rinsed with PBS (5 × 3 min) and incubated for 1 h at 37°C with the following secondary antibodies: anti-human Alexa 488 (Thermo Fisher, Waltham, MA, USA; 1:750) or anti-mouse Alexa 488 (Thermo Fisher ...
-
bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
-
bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
-
bioRxiv - Molecular Biology 2024Quote: Signaling via the intracellular Ca++ pathway was assessed in stably transfected HEK- HEK293/hPTH1R/glosensor (GP-2.3) cells using the calcium-sensitive fluorophore Fura2-AM (Invitrogen, Life Tech. Grand Island, NY, USA) [73 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
-
bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR assays were performed using TaqMan Gene Expression Master Mix and the probes human NEMO/IKBKG (Hs00415849_m1) and human GAPDH (Hs03929097_g1) (ThermoFisher Scientific). Amplifications were run in a Bio-Rad CFX96 real-time PCR system ...
-
bioRxiv - Biochemistry 2020Quote: ... The second incubation were performed with secondary antibody (Anti-Human IgG-Cy3, Jackson ImmunoReserarch; Anti-Human IgM-Cy5, Invitrogen) diluted in ratio 1:1000 in incubation buffer with 1% BSA ...
-
bioRxiv - Cell Biology 2019Quote: Human monocytic U937 cells (ATCC) and human embryonic kidney 293T cells (ATCC) were cultured in RPMI 1640 medium (Gibco), supplemented with 5-10% fetal calf serum (BioWest) ...
-
bioRxiv - Cancer Biology 2021Quote: Human HCC cell lines (Huh7, HepG2) and Human HeLa were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco-BRL) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
-
bioRxiv - Cell Biology 2022Quote: ... Human AFP secreted into the sera of animals were determined by the Human AFP Elisa Quantification Kit (Invitrogen, EHAFP) following manufacturer’s instructions.
-
bioRxiv - Physiology 2022Quote: ... In the analysis of the Woroniecka Human Diabetic Kidney Disease dataset (2011, Affymetrix Human Genome U133A 2.0 Array platform) 33 and the European renal cDNA bank (ERCB ...
-
bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK293T) and human neuroblastoma (SH-SY5Y) cells from ATCC were cultured in high glucose DMEM (Invitrogen) and DMEM-F12 (Invitrogen) ...
-
bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... siRNA targeting human UBE3A or human MASTL (Integrated DNA Technologies) was transfected into cells using Lipofectamine RNAi MAX (Invitrogen). A non-targeting control siRNA was used as a control ...
-
bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
-
bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
-
bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells (HUVECs) and human coronary artery smooth muscle cells (HCASMCs) were purchased from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Molecular Biology 2023Quote: ... human breast cancer MCF7 cells and human bone osteosarcoma U2OS cells were in cultured in DMEM medium (Life Technologies) supplemented with 10% (v/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... Hep-3B human hepatocellular carcinoma cells and MCF7 human mammary carcinoma cells were cultured in Eagle’s Minimum Essential Medium (EMEM) (Gibco) supplemented with 10% heat-inactivated and charcoal stripped fetal calf serum and antibiotics ...
-
bioRxiv - Cancer Biology 2023Quote: ... Normal human melanocytes were grown in Melanocyte Growth Media 254 supplemented with Human Melanocyte Growth Supplement-2 (Life Technologies), calcium chloride (0.3 μM) ...
-
bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
-
bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma HCT-8 cells (ATCC) and THP-1 cells (ATCC) were maintained in Advanced RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1X penicillin-streptomycin (ThermoFisher Scientific ...
-
bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
-
bioRxiv - Neuroscience 2020Quote: ... The next day when cells were at 60–70% confluency and were transduced with 10 µl of lysate normalized to 3ng/µl per well of tau from rTg4510 mice or 5ng/µl per well of tau from human brains plus 1% Lipofectamine 2000 (Invitrogen) diluted in OptiMEM (Thermo Fisher) ...
-
bioRxiv - Cancer Biology 2019Quote: ... were used to retro-transcribe 1 μg of total RNA from Human Liver Total RNA (ThermoFisher Scientific, Waltham, MA, USA). The reverse transcriptase reaction for the viral pellet was carried out with random primers using a commercial kit (EasyScript cDNA Synthesis kit ...
-
bioRxiv - Bioengineering 2020Quote: ... R&D systems sheep anti-human CD31/PECAM-1 antibody (AF806) and Hoechst 33324 (H3570) were obtained from Thermo Fisher Scientific (Whaltam ...
-
bioRxiv - Cancer Biology 2021Quote: ... Virus was produced in 293T cells and human melanoma cell lines were infected and selected with 1 μg/mL Puromycin (Gibco) for 3 days ...
-
bioRxiv - Cell Biology 2021Quote: ... cardiomyocytes were incubated with the secondary antibody diluted in a blocking buffer solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse 594 (1:400 ...
-
bioRxiv - Cancer Biology 2020Quote: ... HDL was labeled with Alexa568 by incubating 4.5 mg Human HDL in 90 μl 1 M sodium bicarbonate with Alexa Fluor 568 succinimidyl ester (A20003, Thermo Fisher) for 1 hr at room temperature (8:1 dye:estimated apoA1 mole ratio) ...
-
bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
-
bioRxiv - Immunology 2021Quote: ... Primary antibodies were diluted 1:100 in PBS-T as follows: single labeling: CD14 (mouse anti-human, 14-149-82, Invitrogen), CD56 (mouse anti-human ...
-
bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were treated with 10 μg/mL mouse monoclonal anti-human IFNAR2 (ThermoFisher Scientific, MMHAR-2), or human IgG (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from human cells (1 x 106 cells) using the TRIZOL reagent according to the manufacturer’s instructions (ThermoFisher Scientific). RNA integrity for each sample was confirmed with by Nano Drop ...
-
bioRxiv - Immunology 2020Quote: ... and human fibroblasts (HF) clone EF-1-F (sourced from Doorbar lab, University of Cambridge) were grown in MEM (Gibco) supplemented with 10 % FBS ...
-
bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM with 1 μg/mL of puromycin (Invitrogen). TMPRSS2 human plasmid (Origene ...
-
bioRxiv - Microbiology 2021Quote: ... on HEK-293T overexpressing the human ACE2 and maintained in the previously described media containing 1 mg/mL of geneticin (Invitrogen) to obtain TMPRSS2/ACE2 HEK-293T cells ...
-
bioRxiv - Cell Biology 2021Quote: ... RWPE-1 (human normal prostate epithelial cells, CRL-11609, ATCC) cells were cultured in Keratinocyte Serum Free Medium (17005042, Gibco) supplemented with 0.05 mg/ml bovine pituitary extract ...
-
bioRxiv - Cell Biology 2021Quote: ... hiPSC-CMs were washed 3 times with PBS 1X and incubated with the secondary antibodies diluted in blocking solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse Alexa Fluor 488 (1:400 ...
-
bioRxiv - Biophysics 2020Quote: Expression was conducted according to the manual of 1-Step Human High-Yield Mini in vitro translation (IVT) kit (Product No. 88891X) distributed by ThermoFisher Scientific (Pierce Biotechnology ...
-
bioRxiv - Developmental Biology 2022Quote: ... These zona-free eggs were incubated with 200 nM Fc-fusion proteins in Biggers-Whitten-Whittingham (BWW) medium for 1 h and then stained with goat anti-human IgG Fc antibody DyLight 488 (Invitrogen) at a dilution of 1:50 for 1 h at 37 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3 times with PBS and incubated for 1 hour with Alexa 488 nm or Alexa 594 nm conjugated secondary antibodies specific for human and rat IgG (Invitrogen) diluted 1:2,000 in 3% BSA in PBS and containing 1 μg/ml DAPI ...
-
bioRxiv - Cancer Biology 2022Quote: ... the ST1 cells were transfected with NICD pCMV Neo/intracellular domain of human Notch1 (NIC-1) plasmid using Lipofectamine 200 (Invitrogen), and then were harvested 48 hours after transfection for western blot and in vitro angiogenesis assay.
-
bioRxiv - Biochemistry 2021Quote: ... The slide was washed thrice with PBS-T for 1 minute and further incubated with goat anti-human IgG (Fc)-Cy5 (Invitrogen) antibodies at 1:2500 dilution ...
-
bioRxiv - Immunology 2019Quote: ... T cells were stimulated for 2 days at a starting density of approximately 1 million cells per mL of media with anti-human CD3/CD28 Dynabeads (ThermoFisher), at a bead to cell ratio of 1:1 ...
-
Peptide Arrays of Three Collections of Human Sera from Patients Infected with Mosquito-Borne VirusesbioRxiv - Microbiology 2019Quote: ... The arrays were then incubated for 1 h with goat anti-human IgG tagged with Alexa Fluor® 488 (Invitrogen) as secondary antibody ...