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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Mouse WD repeat containing protein WRAP73 WRAP73 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... EZQ® Protein Quantitation Kit (Invitrogen™, R33200).
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bioRxiv - Physiology 2024Quote: ... BCA Protein Assay Kit (Thermo Fisher; Waltham, MA) was utilized for protein quantification ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with the Protein Broad Range assay kit (Invitrogen). Trypsin digestion was performed using the S-Trap column digestion protocol42 (ProtiFi) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: An amine-based protein labeling kit (Invitrogen, #A20173) was used for labeling DUNP19 with AlexaFluor-647 or AlexaFluor-594 ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed twice with ELISA wash buffer to remove any excess unbound spike protein and blocked with 2% BSA (ThermoFisher Scientific; 37525) in ELISA wash buffer overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant trimeric ectodomain of S protein with six proline mutations (rtS-ecto6P) for enzyme-linked immunosorbent assay (ELISA) was produced using Expi293F cells (Thermo Fisher Scientific). In addition to the mutation at the furin cleavage site ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatants containing soluble protein were obtained and the concentration of protein determined using A280 readings (Thermo Scientific NanoDrop 2000/2000c spectrophotometer). Poly-G17U and CR1 labeled with a 3’ fluorescein (Dharmacon ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatants containing soluble protein were obtained and the concentration of protein determined using A280 readings (Thermo Scientific NanoDrop 2000/2000c spectrophotometer). Fluorescein-labeled Poly-G17U ...
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bioRxiv - Cancer Biology 2020Quote: ... centrifuged at 12,000 x g at 4°C for 15 min and the supernatant containing the nuclear proteins was transferred into fresh tubes and quantified using the BCA protein assay (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... Total protein quantification of the glass bead protein extracts was conducted using the BCA Protein Assay kit (Thermo Fisher), and samples with equal amount of proteins were applied on SDS-PAGE gels ...
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bioRxiv - Microbiology 2021Quote: Purified ThsA protein was freshly prepared and protein concentration was quantified using Qubit Protein Assay Kit (ThermoFisher cat # Q33212). Protein was diluted in wash buffer to a concentration of 147 ng/μl ...
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bioRxiv - Neuroscience 2019Quote: Membrane proteins and cytosolic proteins were isolated using Mem-PER™ Plus membrane protein extraction kit (Thermo fisher scientific) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... and total protein concentration was quantified using a BCA protein assay (Pierce BCA Protein Assay kit, 23225, ThermoFisher Scientific). Samples were diluted to 0.8 ug/uL with a 1X cell lysis buffer provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the protein extract was then measured with a BCA Protein Assay Kit (Thermo Fisher Scientific). A volume of each protein extract corresponding to 14 mg protein was transferred into a new 5-mL tube containing 150 µL streptavidin beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Protein extracts from cells were assessed for protein concentrations using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Equal amounts of protein lysates were added to SDS-PAGE gels and electrophoresis was performed ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 500 ng ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 2 μg/μL with the IP lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: Protein concentrations were determined using the BCA protein assay (Micro BCATM protein assay kit; Thermo Fisher Scientific, MA, USA) and CLARIOstar microplate reader (BMG labtech Durham ...
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bioRxiv - Pathology 2021Quote: ... The supernatant was moved to a clean tube and incubated overnight with 0.5 μg of either goat anti-mouse KIM-1 or mouse anti-MUC1 (B27.29) antibodies and Protein G conjugated to Sepharose 4B (ThermoFisher). Beads were washed with 0.5 ml HEPES-buffered saline (HBS ...
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bioRxiv - Immunology 2022Quote: ... Purified molecules or mouse anti-human IL-15 antibodies were coated on a MaxiSorp™ 96-well flat-bottom ELISA plate (ThermoFisher Scientific) for 12h and for 72h ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from CM cultivated mycelia for 2 d from three biological repeats using the TRIzol (Invitrogen, USA) reagent ...
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bioRxiv - Cancer Biology 2019Quote: ... were obtained from the ATCC and their identity was verified by Short Tandem Repeat (STR) profiling (AmpFℓSTR Identifiler, Life Technologies), performed by The Centre for Applied Genomics ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative RT-PCR assays were performed with three biological repeats and three technical replications of each experiment using SYBR Green real-time PCR Master Mix (Invitrogen). M ...
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bioRxiv - Genetics 2020Quote: ... 2-3ng of this DNA was used for each RT-qPCR reaction set up with primers specific to the centromeric dh repeat region using SYBR Master Mix (ThernoFisher) in QuantStudio3 (ThermoFisher). Fold enrichment was calculated using ΔΔCt method (27).
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bioRxiv - Biophysics 2020Quote: ... authenticated using a short tandem repeat assay (University of Arizona Genetics Core) and cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 (Gibco, 11320-074) supplemented with 8% fetal bovine serum (Sigma ...
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bioRxiv - Microbiology 2020Quote: The infectivity of lentivirus particles carrying SARS-CoV-2 spike proteins was determined using a vector containing an HIV-1 or MLV long terminal repeat (LTR) that expresses a Gaussia luciferase reporter (Gluc)(50, 51) 293T cells were cultured in DMEM (Gibco) media ...
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bioRxiv - Molecular Biology 2022Quote: ... Reporter plasmid DNAs as well as control reporter plasmid DNAs with no repeats inserted were transfected in HEK293T cells in 24-well plates using Lipofectamine 2000 (ThermoFisher). 16-24 hours after transfection ...
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bioRxiv - Genetics 2023Quote: ... The donor template for homologous recombination was generated by PCR amplification of a human DNA sample carrying 51 CAG repeat into the pCR-Blunt II TOPO plasmid (Invitrogen). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell lines were used in this study after being reauthenticated through short tandem repeat profiling (Applied Biosystems, Massachusetts, USA). All cells were incubated in a 37 °C incubator with 5% carbon dioxide.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Interleukin 6 (IL-6) was measured using an IL-6 ELISA kit following manufacturer’s instruction (Invitrogen, Catalog # CHC1263). Similarly ...
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bioRxiv - Neuroscience 2021Quote: ... The levels of Aβ in these fractions were determined with Aβ40- and Aβ42-specific ELISA kits (KHB3481 and KHB3441, ThermoFisher) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... Total Tau, phospho Tau (pS396, pS199, pT231 and pT181) levels were analysed by Human ELISA Kit from Invitrogen. Cerebral organoids and supernatants (for Aβ ...
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bioRxiv - Cancer Biology 2021Quote: MMP1 in the secretome of cell lines was quantified with a MMP1 Human ELISA Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Cytokine concentrations in culture supernatants were determined using specific ELISA kits (IL-1β and TNFα, Thermo Fisher Scientific) according to instructions provided by the manufacturer.
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bioRxiv - Molecular Biology 2022Quote: ... ERK activation were assessed 20 min after treatment by using ERK1/2 (phospho-T203/Y204) ELISA Kit (Invitrogen) corrected for the amount of total ERK using ERK1/2 (Total ...
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bioRxiv - Bioengineering 2021Quote: ... The following cytokines were measured according to the instrzctions of each ELISA kit: TNFα (Invitrogen, #88-7346-88), IFNγ (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: BMP4 concentration released into the media was quantified using the BMP-4 Human ELISA Kit from (ThermoFisher, EHBMP4). Standards and protocols were done according to the instructions of the kit ...
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bioRxiv - Immunology 2021Quote: ... and anti-Spike IgG was quantified using a Human SARS-CoV-2 Spike (Trimer) IgG ELISA Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Hypoxia-inducible factor 1-alpha (HIF-1α) was evaluated using a HIF-1 Alpha ELISA Kit (Invitrogen™). Procedure steps were followed according to the manufacturer’s instructions and results were monitored at 450 nm using a microplate reader (iMARK™ ...
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bioRxiv - Cancer Biology 2022Quote: ... TGF-β levels (pg/mL) were determined using a commercial murine ELISA kit (#BMA608-4; Invitrogen, Massachusetts, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... according to the manufacturers’ instructions: Enzo® Life Sciences Direct cAMP ELISA kit (ADI-900-066, Fisher Scientific) and Invitrogen™ PKA Colorimetric Activity kit (EIAPKA ...
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bioRxiv - Synthetic Biology 2023Quote: Quantification of TNF-α was performed on undiluted samples using a porcine TNF-α ELISA kit (Invitrogen, USA) with 20 pg/mL sensitivity according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total human IgG levels were determined following the manufacturer’s instructions in the Human IgG Total ELISA Kit (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... IL-1β secretion from PMA-THP1 was detected using IL-1 beta Human ELISA kit (#KHC0011, ThermoFisher Scientific) and IL-1 beta Human ELISA kit ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The quantitative analysis of Aβ40 and Aβ42 was performed using an ELISA kit according to the manufacturer’s instructions (Life Technologies). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... The plasma insulin concentration was determined using a Human Insulin ELISA kit according to the manufacturer’s protocol (Invitrogen). The relative bioavailability (RA ...
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bioRxiv - Bioengineering 2023Quote: The following enzyme-linked immunoassay (ELISA) kits were used to analyze secreted factors: IL-18 (BMS267-2, Thermofisher) and IL-8 (D8000C ...
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bioRxiv - Immunology 2023Quote: ... IL-18 and human IL-1β was analyzed in the cell culture supernatant by commercial ELISA kits (Invitrogen) following the manufacturer’s recommended procedures.
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bioRxiv - Neuroscience 2023Quote: Serum concentrations of CRP and IL-6 were determined using a commercially available ELISA kit (Thermo Fisher, USA) according to the manufacturer’s instructions ...