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Citations for Thermo Fisher :
1751 - 1800 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... were grown in YPD to early stationary phase and stained with 0.5 µg/ml BODIPY 493/503 (Invitrogen) for 5 min at RT ...
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bioRxiv - Immunology 2021Quote: ... The aqueous phase was transferred into a new tube and RNA was mixed with 1ul of GlycoBlue (Invitrogen) and precipitated with 250 μL isopropanolol ...
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bioRxiv - Genetics 2021Quote: ... DNA was simultaneously isolated from the organic phase using the back extraction protocol as described by Thermo Fisher Scientific (see S1 Methods for details) ...
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bioRxiv - Immunology 2021Quote: ... Reversed-phase liquid chromatography was performed using a Dionex RSLCnano high-performance liquid chromatography system (Thermo Fisher Scientific). Peptides were injected onto a 75⍰μm⍰×⍰2⍰cm PepMap-C18 pre-column and resolved on a 75⍰μm⍰×⍰50⍰cm RP C18 EASY-Spray temperature-controlled integrated column-emitter (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The labeled sample was fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) and subject to liquid chromatography–MS/MS analysis using Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient with a flow rate of 300 nl min−1 ...
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bioRxiv - Cancer Biology 2021Quote: High pH reverse phase (HPRP) fractionation was performed using a Dionex UltiMate 3,000 RS pump (Thermo Fisher Scientific) on an Acquity UPLC CSH C18 1.7 μm ...
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bioRxiv - Plant Biology 2020Quote: ... The final residue was dissolved in 5% Acetonitrile - 1% Acetic acid and separated by reverse phase UPHL chromatography (2.6 µm Accucore RP-MS column, 100 mm length x 2.1 mm i.d.; ThermoFisher Scientific) with a 5% to 50% acetonitrile gradient ...
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bioRxiv - Biochemistry 2020Quote: ... soluble phase was isolated after centrifugation of the whole-cell lysate in a T-647.5 rotor (Thermo Fisher) at 40,000 rpm for 30 minutes at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Nano-LC mobile phase was introduced into the mass spectrometer using a Nanospray Source (Thermo Scientific, Waltham, MA). During peptide elution ...
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bioRxiv - Neuroscience 2020Quote: ... Peptides were separated by reverse-phase chromatography on a 50cm EASY-Spray C18 LC column (Thermo Fisher Scientific) connected to an EASY-nLC 1200 instrument equipped with an autosampler (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Tryptic peptides were separated by reverse-phase liquid chromatography on an easy-nLC 1000 system (Thermo Fisher Scientific) and directly sprayed into a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were desalted using Hypersep Tip C18 (10-200μL) microscale solid phase extraction tips (Thermo Scientific, Rockwood, TN). Prior loading of the tryptic digest ...
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bioRxiv - Molecular Biology 2022Quote: ... A cleanup of the sscDNA containing aqueous phase is completed using Dynabeads MyOne Silane beads (Life Technologies 37002D). The second strand cDNA amplification is performed using the cDNA Amplification Mix on the Eppendorf MasterCycler Pro ...
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bioRxiv - Neuroscience 2022Quote: ... and fractionated using the Pierce High-pH Reversed-Phase Peptide Fractionation (HPRP) Kit (Thermo Fisher Scientific, Rockford, IL) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Cell Biology 2022Quote: ... HUDEP2 cells were cultured for three days (phase 1) in differentiation medium composed of IMDM base medium (GIBCO) supplemented with 2% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Neuroscience 2020Quote: ... Tryptic peptides were analysed by a reversed-phase capillary liquid chromatography system (Ultimate 3000 nanoLC system; Thermo Scientific) connected to an Orbitrap Elite mass spectrometer (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... and 50% ACN with 0.1% triethylamine from Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific, USA).
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bioRxiv - Biochemistry 2021Quote: ... in water and mobile phase B consisted of 60/40/0.1% isopropyl alcohol/tetrahydrofuran/TFA (Thermo Fisher Scientific). Lipids were eluted by step-gradient with an initial 1.5-minute hold at 5% B ...
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bioRxiv - Microbiology 2022Quote: ... Peptide separation was performed using a reverse-phase analytical column (Acclaim PepMap RSLC, 0.075 × 250 mm, Thermo Scientific) with a linear gradient of 4 %-36 % solvent B (0.1 % FA in 98 % ACN ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated into eight fractions using a High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Desalted peptide samples were analysed by a reversed-phase capillary nano liquid chromatography system (Ultimate 3000, Thermo Scientific) connected to a Q Exactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... and 90 to 20% of mobile phase B (water + 0.05% formic acid) into a Syncronis™ C18 column (50×2.1 mm, 1.7μm, Thermo Scientific) during 23 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and 6.4 µl were analysed by a reversed-phase capillary nano liquid chromatography system (Ultimate 3000, Thermo Scientific) connected to an Orbitrap Velos mass spectrometer (Thermo Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... coupled to a Proxeon Easy nLC 1000 reversed-phase chromatography system (Thermo Fisher Scientific, San José, CA, USA) using a binary solvent system consisting of solvent A and solvent B (0.1% FA in ACN) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The upper aqueous phase was transferred to a 2 ml screw top microcentrifuge tube (Fisher Scientific, Hampton, NH) and 0.1X volume of 3M sodium acetate and 1X volume of isopropanol were added and the tube inverted until DNA aggregation ...
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bioRxiv - Physiology 2021Quote: ... The mobile phase consisted of two solutions: eluent A (H2O + 0.1% Formic acid, MS grade, Thermo Scientific™) and eluent B (acetonitrile + 0.1% Formic acid ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... using a capillary BioBasic C18 reversed-phase column (BioBasic-18, 150 × 0.32 mm, 5 μm, Thermo Fisher Scientific), installed in a Dionex Ultimate U3000 HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... and also visualized on glass slides under phase contrast microscopy (EVOS FL Auto, 40x and 100x Objective, ThermoFisher). Voltages for flow cytometry were fixed throughout ...
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bioRxiv - Microbiology 2020Quote: ... Eluates were combined prior to fractionation with the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Scientific). The dried TMT-labelled phosphopeptide fractions generated were resuspended in 0.1% TFA for LC-MS/MS analysis using a U3000 RSLCnano system (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Reversed-phase liquid chromatography was performed on a Thermo Scientific™ EASY-nLC 1000 system (ThermoFisher Scientific, USA) on-line coupled to a Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ mass spectrometer (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... U87 cells in the phase of logarithmic growth were digested with trypsin (Gibco Biotech Co, Ltd, Beijing, China), collected ...
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bioRxiv - Immunology 2022Quote: ... 1 μg was injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) then electrosprayed into an Orbitrap mass spectrometer (LTQ Orbitrap Velos Pro ...
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bioRxiv - Immunology 2022Quote: ... Reversed-phase liquid chromatography was performed using a Dionex RSLCnano high-performance liquid chromatography system (Thermo Fisher Scientific). Peptides were injected onto a 75 μm × 2 cm PepMap-C18 pre-column and resolved on a 75 μm × 50 cm RP C18 EASY-Spray temperature-controlled integrated column-emitter (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... KPC cells with or without K17 expression were harvested during log-phase growth and resuspended in DMEM (Gibco) with Matrigel (Life Sciences ...
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bioRxiv - Developmental Biology 2022Quote: ... Peptides were then desalted by reverse-phase chromatography using Pierce peptide desalting spin columns (Thermo Fisher part #89852). Final resuspension was done with Solvent A (0.1% formic acid in Fisher Optima LC/MS grade water ...
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bioRxiv - Molecular Biology 2022Quote: ... After separation of the phases the RNA was precipitated by the addition of 0.75 μl GlycoBlueTM Coprecipitant (ThermoFisher), 40 μl 3 M sodium acetate pH 5.5 and 1 ml 100% ethanol ...
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bioRxiv - Biochemistry 2022Quote: ... and mobile phase B comprised acetonitrile with 0.1% formic acid (v/v) (Thermo Fisher Scientific; Cat. # LS120-500). The peptides were separated using linear gradient elution with 0–35% mobile phase B for 240 min and 35–100% for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 mins organic gradient ...
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bioRxiv - Molecular Biology 2022Quote: ... after which they were examined and photographed using an EVOS FL fluorescence phase contrast microscope (Thermo Fisher Scientific). All results were obtained from three independent experiments.
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bioRxiv - Plant Biology 2022Quote: ... Peptides were subsequently fractionated using high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific, San Jose, USA) and pooled into 8 fractions [43] ...
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bioRxiv - Systems Biology 2024Quote: ... peptide mixtures were separated by a reversed-phase chromatography (Dionex Ultimate 3000 NCS-3500RS Nano, Thermo Fisher Scientific) coupled on-line to an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Organic phases were combined and evaporated to dryness using a SpeedVac Savant centrifugal evaporator (Thermo Scientific, Dreieich, Germany). Proteins were extracted from the residue debris pellets and quantified using a BCA kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Eluates were combined prior to fractionation with the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Scientific). The dried TMT-labelled phosphopeptide fractions generated were resuspended in 0.1% TFA for LC-MS/MS analysis using a U3000 RSLCnano system (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: LC-MS/MS was carried out by nanoflow reverse phase liquid chromatography (Dionex Ultimate 3000, Thermo Fisher Scientific) coupled online to a Q-Exactive HF Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1 % formic acid) and purified in five successive reverse phase HPLC runs using an Ultimate 3000 (ThermoFisher Scientific) equipped with a Kinetex 5μ XB-C18 100A ...
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bioRxiv - Immunology 2024Quote: ... Reversed-phase liquid chromatography was performed using a Dionex RSLCnano high-performance liquid chromatography system (Thermo Fisher Scientific). Peptides were injected onto a 75 μm × 2 cm PepMap-C18 pre-column and resolved on a 75 μm × 50 cm RP C18 EASY-Spray temperature-controlled integrated column-emitter (Thermo Fisher Scientific ...