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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... 20 μL of 20% (v/v) alamarBlue® reagent (ThermoFisher Scientific) was added to each well without removing the pre-existing media ...
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bioRxiv - Neuroscience 2023Quote: ... 20 units/ml penicillin and 20 μg/ml streptomycin (ThermoFisher, 15140122) in T75 flasks (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µL of 20 mg/mL proteinase K (Thermo Fisher, EO0491) was added to the digestion solution and incubated at 55°C for 2 hrs ...
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bioRxiv - Genetics 2019Quote: We extracted the RNA of these 20 hybrid samples using the TRIzol Plus RNA Purification Kit (Invitrogen). Transcriptome libraries were made by the Smart-seq2 protocol (Picelli et al ...
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bioRxiv - Genomics 2019Quote: ... stephensi mosquitoes (20 no.) and cDNA was synthesized using Verso cDNA synthesis kit (Thermo Fisher Scientific, #AB1453A) as per manufacturer protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was quantified and reverse transcribed using oligo(dT)20 primers and the SuperScript IV kit (ThermoFisher). RT-qPCR was performed on the LightCycler 480 II instrument (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µl isolated RNA was treated with DNase using the DNA-free Kit (Ambion, Carlsbad, CA, USA). RNA quality and quantity were determined on the Agilent 2100 Bioanalyzer platform (RNA 6000 Nano ...
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bioRxiv - Microbiology 2023Quote: ... cells were stained for 20 min at RT with the Fixable Blue Dead Cell Stain Kit (Invitrogen) to determine viability ...
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bioRxiv - Systems Biology 2023Quote: ... The amplified PCR products were then normalized to 20 µL using a SequalPrep Normalization kit (Life Technologies). Aliquots of 5 µL from each normalized sample were subsequently pooled into the final library ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 μL of eluted RNA was treated with TURBO DNA-freeTM Kit (Invitrogen, MA, The US, #AM1907) by using routine DNase treatment protocol provided by the supplier ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Microbiology 2022Quote: ... We extracted the genomic DNA of ELISA-positive cattle using either the magLEAD 12gC or a MagMAX™ CORE Nucleic Acid Purification Kit (Thermo Fisher Scientific Inc., Waltham, USA) with an automated nucleic acid extraction system (KingFisher Duo Prime ...
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bioRxiv - Neuroscience 2023Quote: TNFα and IL-6 released in the microglia conditioned medium were quantified using mouse TNFα and IL-6 ELISA kits according to manufacturer’s instructions (Invitrogen, 88-7324 and 88-7064-22, respectively) and a SpectraMax i3x spectrophotometer (Molecular Devices) ...
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bioRxiv - Immunology 2020Quote: ... were co-cultured in 96 well well plates at 1:1 E:T ratio with Raji tumor targets pre-labeled with Vybrant DyeCycle Ruby Stain (ThermoFisher) and CellTracker Deep Red Dye (ThermoFisher) ...
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bioRxiv - Genomics 2019Quote: ... with 1% beta-mercaptoethanol for Smart-seq2 and 384-well plates containing 0.6 µl 1% NP40 (Thermo Fisher Scientific) for CEL-Seq2.
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bioRxiv - Microbiology 2020Quote: Cells listed in Sup.Table.1 were seeded at a density of 1×105 cells/well in a 24-well plate (Nunc) and 24h later infected with SARS-CoV-2 England-2/2020 ...
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bioRxiv - Immunology 2022Quote: ... and HFs were placed individually into a 96-well plate slot with 100 µl supplemented medium (William’s E plus 1% penicillin-streptomycin antibiotics, 1% L-glutamine (Invitrogen-Gibco), 0.01% hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: Monolayer cultures of patient-derived GMB TPCs were propagated on Matrigel-coated plates in DMEM:F12 Neurobasal Medium (1:1; Gibco), 1% B27 supplement (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were trypsinized and transferred (1 200-1 800 cells/ well) to 96-well Nunclon Sphera plates (Thermo Scientific) for gene expression ...
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bioRxiv - Immunology 2020Quote: ... in quadruplet were prepared in 96-well round-bottom plates using Dulbecco’s Modified Eagle Medium (DMEM) with 1% (v/v) FBS and 1% Antibiotic-Antimycotic (Gibco). 75 µL of the diluted sera was mixed with an equal volume of media containing 64 PFU of SARS-CoV-2 virus (hCoV-19/England/02/2020 ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were seeded on 0.1% gelatin-coated plates and NPCs are cultured in N2B27 medium (1:1, Neurobasal:DMEM-F12, supplemented with N2 and B27, ThermoFisher) in the presence of 10 ng/ml bFGF (Peprotech ...
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bioRxiv - Bioengineering 2023Quote: ... while non-BrdU plates were stained with calcein AM and ethidium homodimer-1 Live/Dead stains (Invitrogen, 1:1000) to quantify living and dead cells ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel attached were transferred to a well of a 6-well plate with 1 ml of digestion solution (0.5% Triton X100, 1 mM EDTA pH 8 (AM9260G, Ambion), 50 mM Tris pH 8 (AM9855G ...
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bioRxiv - Cell Biology 2024Quote: ... mES cells were cultured on 0.2% gelatin coated plates in 2i/LIF containing medium (1:1 mix of DMEM/F12 (Givco) and Neurobasal (Gibco) supplemented with 1x Pen-Strep (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel stuck to it were transferred to a 6-well plate with 1 ml of digestion solution (0.5 % Triton X100, 1 mM EDTA, pH 8 (AM9260G, Ambion), 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... mES cells were cultured on 0.2% gelatin coated plates in 2i/LIF containing medium (1:1 mix of DMEM/F12 (Gibco) and Neurobasal (Gibco ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: Flash-frozen brain tissue was homogenized with a mechanical handheld homogenizer for 20 seconds in RNA kit lysis buffer (PureLink™ RNA Mini Kit, Life Technologies, Carlsbad, CA) plus 1% β-mercaptoethanol ...
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bioRxiv - Genetics 2021Quote: ... The cells were transfected with 500ng of pDG459 (Step 1) or pX459 and ssODN templates at a 1:20 molar ratio using 2μL of Lipofectamine Stem (ThermoFisher #STEM00003) per well ...
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bioRxiv - Bioengineering 2020Quote: ... the fixed hydrogels were incubated in PBS with 1% bovine serum albumin and 0.5% Tween-20 for 30 minutes and then with 1:40 rhodamine-phalloidin (ThermoFisher Scientific) overnight ...
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bioRxiv - Genomics 2022Quote: ... for 1 h at 37°C and then diluted 1:20 in 50 μg/mL yeast tRNA (cat #Am7119, Invitrogen). A known concentration of plasmid DNA used to prepare the virus was also diluted 1:200 in 50 μg/mL yeast tRNA and processed in parallel ...
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bioRxiv - Neuroscience 2022Quote: ... was dissolved in 0.9% sterile Saline and pH was adjusted to 7.4 with 20-30 µl 1 N NaOH (#SS266-1, Fisher Scientific) according to Tocris Bioscience’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... clarified transfection supernatant was diluted 1/4 in a 20 μl volume of transfer buffer (Life Technologies Cat# NP0006-1) and applied dropwise to a presoaked 0.45 μm PVDF membrane ...
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bioRxiv - Neuroscience 2020Quote: ... The PFC was dissected out from the sections and the tissue stored in 1 mL ice-cold Leibovitz’s L-15 medium containing 1 μL SUPERase RNase inhibitor (20 U/μl, ThermoFisher Scientific). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... followed by washing with Tween-20/PBS and 1 h incubation with HRP-conjugated goat anti-mouse IgG at 1:40 000 (Cat# A16066, Invitrogen) in 100 µl per well of Tween-20/PBS ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... Soluble chromatin supernatant equivalent to 6 μg per IP was pre-cleared using 20 μl of a 1:1 mix of protein A and protein G Dynabeads (Invitrogen). Overnight IP was performed using 1 μg of anti-H3K4me3 (Diagenode ...
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bioRxiv - Bioengineering 2022Quote: ... CD34+ iPSC-EP-laden hydrogels were incubated in blocking buffer (DPBS supplemented with 0.1% Tween-20 and 1% BSA) for 30 minutes and then with 1:40 rhodamine-phalloidin (ThermoFisher Scientific) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... and rat anti-Ecad (DEcad, 1:20) were from the Developmental Studies Hybridoma Bank, rabbit anti-GFP (A11122, 1:1000) from ThermoFisher, chicken anti-GFP (ab13970 ...
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bioRxiv - Genomics 2022Quote: ... resuspended in 1 mL NSB with 1:20 dilution of 0.25 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) and FACS sorted ...
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bioRxiv - Cell Biology 2023Quote: ... was prepared in secondary antibody buffer (0.1% Tween 20 + 0.5% BSA in PBS) also containing Hoechst (1:2000) and Alexa fluor 488-phallodin (A12379, Thermo Fisher) (1:1000 ...
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bioRxiv - Genetics 2023Quote: Previously purified His-MBP-TEV-DNMT3B213-555 proteins were diluted to 1.5 μM in normalised TDA buffer (20 mM HEPES, 150 mM NaCl, 1 mM DTT, 0.5% glycerol, 1 μM ZnCl2, 5x SYPRO orange from Life Technologies). 50 μl reactions were imaged in triplicate in a 96-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated in primary antibody with 5% goat serum in PTX, mouse-nc82 (1:20) (Development Studies Hybridoma Bank, University of Iowa) and rabbit-antiGFP (1:150) (Invitrogen) in PTX for 48 hours in 4°C while rotating ...
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bioRxiv - Immunology 2024Quote: ... 180 µl of the 1:10 diluted supernatants were mixed with 20 µl of the 1 µg/ml Coelenterazine substrate (Invitrogen), and luciferase activity within the mixture was measured in duplicates with Lumat LB 9507 (Berthold Tech ...
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bioRxiv - Developmental Biology 2024Quote: ... and cultured in SCs culture medium (growth medium: 20% FBS, 5% horse serum, 1% penicillin/streptomycin, 1% GlutaMAX supplement (Gibco) and 2.5 ng/ml FGF (R&D systems ...
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bioRxiv - Cell Biology 2023Quote: ... Ribosome profiling was performed as previously described (53) except that 1 U/20 x 106 cells RNase 1 (Thermo Scientific) was used and rDNA depletion was performed using biotinylated rDNA sequences (47) ...
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bioRxiv - Cell Biology 2020Quote: ... MEF plates were used within 1 week and washed with DPBS (ThermoFisher Scientific 14190250) prior to plating of ESC/D-EPSCs in appropriate media.
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bioRxiv - Immunology 2021Quote: ... plates were washed and developed with streptavidin-HRP (1:1000; ThermoFisher Scientific, Pittsburgh, PA) and TMB ...
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bioRxiv - Microbiology 2020Quote: ... plating on BHI agar plates containing 10 μg mL−1 erythromycin (Erm; Fisher Scientific). A gene SOEing product merging the two flanking regions of relP was then generated ...
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bioRxiv - Immunology 2020Quote: ... cells were stimulated with 1 µg/mL plate bound anti-CD3 (UCHT1, Thermo Fisher Scientific Cat# 16-0038-85 ...
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bioRxiv - Genomics 2020Quote: ... cells were lysed directly in the plate by adding 1 ml of Trizol (Invitrogen). RNA was isolated using the Direct-Zol RNA Miniprep Kit (Zymo Research ...