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Citations for Thermo Fisher :
1701 - 1750 of 7821 citations for LRP12 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... HEK 293T/17 (ATCC CRL-11268) cells were plated and co-transfected with Lipofectamine 3000 (L3000015 Invitrogen), 3rd generation packaging vectors pRSV-REV #12253 ...
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bioRxiv - Cell Biology 2020Quote: ... 1.3×105 HEK-293T cells per cm2 were reverse transfected with Lipofectamine® 2000 (Thermo Fisher Scientific) following manufacturer’s instructions using 200 ng psiCHECK™ 2 (Promega ...
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bioRxiv - Biophysics 2021Quote: A commercial HEK cell suspension cell line optimized for protein expression Expi293-F (Cat. No. A14635, Gibco Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... HEK-293E/HeLa cells were transfected with indicated constructs 2 days before experiments using lipofectamine2000 (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: HEK-293T cells (ATCC CRL-3216) are maintained in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher 10569044) supplemented with 10% fetal bovine serum (Thermo Fisher 26140079 ...
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bioRxiv - Molecular Biology 2019Quote: Jurkat T cells and HEK 293T (CRL-3216, ATCC) were maintained in RPMI (21875-034, Life Technologies) supplemented with 10% FCS (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK-293T cells were transfected with the anti-Chi3l1 construct using Lipofectamine™ 3000 (Invitrogen, L3000015, CA). Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: HeLa and HEK 293T cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... 3×106 HEK-293T cells were seeded in 10ml of complete DMEM Dulbecco’s Modified Eagle’s Medium (Gibco) supplemented with 10% FBS and 50μg/ml penicillin-streptomycin ...
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bioRxiv - Cell Biology 2024Quote: ... while HEK-293T and Vero E6 cells were cultured in DMEM medium (Dulbecco’s Modified Eagle Medium; Gibco). Both media were supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: HEK cells and zebrafish samples were tagged with TMT10plex™ Isobaric Label Reagent Set (Thermo Scientific, #90111), and fibroblast cells with TMTpro™16plex Isobaric Labels (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: All DNA plasmids were transfected into Hek-Luc cells using Lipofectamine™ LTX Reagent (Thermo Fisher Scientific). Cells were seeded 24 hours prior to transfection to ensure high viability and suitable confluency ...
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bioRxiv - Bioengineering 2023Quote: HEK-293T or Neuro 2A cells were cultured on 12-mm cover glass (Fisher Scientific, Pittsburgh, PA) coated with fibronectin (Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: ... HEK-293FT cells were cultured in DMEM medium supplemented with GlutaMAX-I (Thermo Fisher Scientific, 10569-010), 1× MEM Non-Essential Amino Acids (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... required pLL5.0 expression vectors and packaging vectors were transfected into HEK-293T cells using Lipofectamine 2000 (Invitrogen). 24 hrs after transfection cell medium was changed to fresh DMEM supplemented with 10% serum ...
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bioRxiv - Neuroscience 2023Quote: ... HEK 293T cells were transiently transfected with cDNA constructs encoding proteins of interest using Lipofectamine 2000 (Invitrogen) 16-24 hours before the start of the experiment ...
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bioRxiv - Molecular Biology 2023Quote: MCF-7 and HEK-293T cells were obtained from ATCC and maintained in high glucose DMEM (Invitrogen) at 37℃ with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... HEK-293T cells were transfected with the bivalent FRGxCTLA-4 construct using Lipofectamine™ (Invitrogen, CA, USA). The bispecific antibody was purified from the collected supernatant using a protein A column (Thermo Scientific # 89960 ...
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bioRxiv - Immunology 2024Quote: ... CyRPA protein were expressed as secreted protein by transient transfection of HEK Expi293 cells (Thermo Fisher Scientific) following the manufacturer’s protocol using ExpiFectamine™ (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2024Quote: ... HEK 293T cells were transiently transfected with indicated constructs using the lipo2000 (1 mg/ml, LIFE technologies) reagents ...
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bioRxiv - Neuroscience 2024Quote: HEK-293T or Neuro 2A cells were cultured on 12-mm cover glass (Fisher Scientific, Pittsburgh, PA) coated with fibronectin (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Suspension culture of HEK-293T cells was cultivated in the 1:1 mixture of FreeStyle F17 (Gibco) and EX-CELL 293 media ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Cell Biology 2019Quote: ... 293-T-REx™ cells were cultured in the presence of 5 µg/mL blasticidin (Gibco; #A11139-03) to maintain the pcDNA™6/TR plasmid carrying the ORF for tetracycline (Tet ...
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bioRxiv - Cell Biology 2021Quote: Human Embryonic Kidney 293 (HEK293) cells (ATCC CRL-1573) were cultured in complete growth Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with 10% fetal bovine serum (UCSF Cell Culture Facility) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were produced by transient co-transfection of exponentially growing Freestyle™ 293-F suspension cells (Thermo Fisher) using polyethylenimine (PEI)-precipitation method as previously described (Lorin and Mouquet ...
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bioRxiv - Immunology 2022Quote: ... Recombinant antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described (Lorin and Mouquet ...
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bioRxiv - Cell Biology 2022Quote: HEK TREx-293 and HuH7 cell lines used in this study were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) with 10% fetal bovine serum (Qualified FBS ...
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bioRxiv - Neuroscience 2020Quote: ... and the cells were stimulated with 5 μM A23187 in 400 μL FreeStyle 293 Expression medium (Thermo Fisher) per well ...
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bioRxiv - Genetics 2021Quote: ... Flp-In T-REx 293 cells AUF1 cell line was cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) and supplemented with 10% Heat-Inactivated Fetal Bovine Serum (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were made by transfecting the IgG1 expression construct using the ExpiFectamine™ 293 Transfection Kit (ThermoFisher) in Expi293F (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... Expi293F cells were transfected with the pAdd2 5FnIII construct using the ExpiFectamine 293 kit (Thermo Fisher Scientific A14524) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was first packaged in 293 FT cells using co-transfection with LV-MAX Lentiviral packaging mix (ThermoFisher) for 48 hrs ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells (2.5×106 cells/ml) were transfected using ExpiFectamine™ 293 transfection reagent (ThermoFisher, cat. no. A14524). The cells were harvested 4-5 days post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK293 cells were cultured in a 2L suspension using FreeStyle 293 Expression Medium (Life Technologies, Carlsbad, CA, USA) with a cell density maintained at 1 × 10^6 cells/mL on the day of transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293F cells were maintained in suspension within Freestyle™ 293 Expression Medium (#12338026, ThermoFisher Scientific, Waltham, MA, USA) at a temperature of 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: Munc13 was expressed in ExpiHEK-293 cell cultures using ExpiFectamine as a transfection reagent (Thermo Fisher, Waltham, MA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Munc13C was expressed in ExpiHEK-293 cell cultures using ExpiFectamine as a transfection reagent (Thermo Fisher, Waltham, MA). Pellets were resuspended in lysis buffer and lysed using a Dounce homogenizer ...
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bioRxiv - Biochemistry 2023Quote: ... pcDNA5FRT-SNAP was co-transfected with pcDNA5FRT-Halo to the original Flp-In™-293 Cell Line (Invitrogen); and for positive control experiments ...
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bioRxiv - Systems Biology 2023Quote: ... 2.5× 106 cells were transfected with 10.8µg DNA using the ExpiFectamineTM 293 Transfection Kit (Thermo Fisher Scientific, A14525) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... plasmids were transfected into 293 T cell or Hela cell using Lipofectamine™ 3000 Transfection Reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293S GnTI− cells (ATCC CRL-3022) were grown in suspension at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Immunology 2024Quote: Recombinant rabbit mAbs and Fabs were expressed in HEK293F cells using ExpiFectamine™ 293 Transfection reagent (Gibco, A14525). Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (V/V) fetal bovine serum (HI FBS heat inactivated, Gibco) and GlutaMAX-I (Gibco)39 ...
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bioRxiv - Microbiology 2019Quote: ... 1x Pen/Strep/Glu and 10% ultralow IgG HI-FBS (Thermo Fisher Scientific), and pelleted at 350 xg for 5 min ...
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bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat ...
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bioRxiv - Immunology 2021Quote: ... HI sera were tested using PrioCHECK PRRSV Antibody ELISA Kit (Thermo Fisher Scientific) for detection of PRRSV N protein-specific antibodies ...