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Citations for Thermo Fisher :
1701 - 1750 of 7360 citations for HDGF2 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and into pVL847 (His-MBP-tag overexpression vector) by Gateway cloning method (Invitrogen). ΔnahA mutant of Bacillus subtilis was constructed by CRISPR/Cas9 editing method (Ref) ...
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bioRxiv - Molecular Biology 2021Quote: Western blot analysis was performed using an anti-His antibody (Invitrogen, LOT 1902132) to confirm the identity of splicing and cleavage products ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Thermo Fisher Scientific) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) in 1x PBS for 6 hours at ambient temperature and blocked overnight with 0.5% NCS/5% Goat Serum/5% Milk/0.2% PBS-T ...
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bioRxiv - Immunology 2021Quote: ... or left unstimulated in 10% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) containing Brefeldin A (eBioscience cat ...
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bioRxiv - Microbiology 2021Quote: ... gingivalis were stained with 15 mg/l hexidium iodide (HI; Thermo Fisher Scientific) and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC ...
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bioRxiv - Cell Biology 2021Quote: ... before being incubated with the primary antibody (6x-His Tag Monoclonal Antibody, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and His-tagged mCherry (strain He/80/FR) were synthesized by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (hi-FBS, Gibco, 16140-071), 100U/mL Penicillin/100ug/mL Streptomycin (pen/strep ...
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bioRxiv - Plant Biology 2022Quote: ... the secondary antibody used was anti-6x-His tag monoclonal (Invitrogen, # MA1-21315) with 1:100 dilutions ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was then purified using Ni-NTA His affinity agarose (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 6x-His Epitope Tag Monoclonal Antibody (#MA1-21315, Thermofisher Scientific; dilution 1:500) was used for His-tag staining of the TRPA1 channel in fixed cells for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... each experiment sample was separated on a Hi-pH column (Thermo Fisher Scientific) according to the vendor’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μl of Dynabeads™ His-Tag Isolation and Pulldown beads (ThermoFisher 10104D) for each CFPS reaction were equilibrated twice in equal volumes of wash buffer before addition to the purified reactions to remove the Ulp-protease and incubated with spinning at 4 °C for at least 30 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with an anti-His antibody with HRP conjugate (Invitrogen) in 1% BSA at 4°C overnight with gentle rocking ...
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bioRxiv - Genetics 2023Quote: ... Libraries were mixed with Novex 5X Hi-Density TBE Sample Loading Dye (Thermofisher), then loaded into a 6% TBE acrylamide gel with the Quick-Load pBR322 DNA-MspI Digest ladder and run for 65 minutes at 120 V ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% (v/v) HI-FBS and N-2 supplement (Gibco™). ATM knockout (KO ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (hi-FCS) (Gibco) and 1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Western blots were carried out probed with anti-His mAb (1:1000, Invitrogen) followed by anti-mice IgG-HRP (1:20000 ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting was performed using anti-His antibody (Thermo Fisher, Cat. No. MA1-21315) at a dilution of 1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... neutralized by adding RPMI-1640 with 10% heat inactivated FBS (HI FBS, Gibco), and spun down at 200 x g for 10 minutes to pellet the cells ...
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bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
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bioRxiv - Cancer Biology 2022Quote: ... medium containing 10% (v/v) heat inactivated fetal calf serum (HI-FCS; Gibco), 100 U/mL Penicillin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10 % v/v heat-inactivated fetal calf serum (HI-FCS, Gibco), 1 mM non-essential amino acids (NEA ...
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bioRxiv - Synthetic Biology 2024Quote: ... then diluted at 1:100 in Hi-Def Azure Media (Fisher Scientific # 3H5000) supplemented with 1% Glucose and various concentrations of peptide in 100 µl in a 96well plate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were placed in HI buffer containing 1:1,000 CellMask Deep Red (Invitrogen) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... excluding the HI-FBS and supplementing with 0.1% BSA (Life Technologies 15260-037). CHIM was used for the experiments in Figures 4 ...
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bioRxiv - Microbiology 2024Quote: ... Bio-His-FIKK13 was incubated with different quantities of magnetic streptavidin beads (Invitrogen) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Immunology 2021Quote: Ab heavy and light plasmids were co-transfected at a 1:1 ratio into HEK-293F cells (Thermofisher) using PEI Max (1 mg/mL ...
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bioRxiv - Microbiology 2019Quote: ... bone marrow-derived macrophages (BMM) Vero and HEK 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplemented with 10% foetal calf serum (FCS ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: HEK-293FT cells and HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: ... Both COS-7 and HEK 293T were transfected by means of Lipofectamine and PLUS reagent (Thermo Fisher Scientific). The calcium phosphate transfection method was instead used to transfect WT or ΔOLF Lphn2-HA-pCCL in HEK 293T cells and to produce the corresponding viral particles subsequently employed to transduce ECs ...
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bioRxiv - Microbiology 2021Quote: ... and human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in flasks containing Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: HEK-293T cells (purchased at ATCC, CRL-3216) were grown in complete Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... eight pHW2000 plasmids encoding all viral segments were transfected into HEK 293T cells using Lipofectamine 2000 (Thermo Scientific). Plaque assays of viral rescue supernatants were performed on MDCK-II cells to generate single plaques used for further stock generation on MDCK-II cells ...
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bioRxiv - Immunology 2022Quote: ... or its truncation mutant were single- or co-transfected into HEK 293T cells by Lipofectamine LTX (ThermoFisher, 15338100) for 16 h ...
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bioRxiv - Biochemistry 2020Quote: U2AF2 levels were reduced by transfection of HEK 293T cells with Stealth™ siRNA HSS117616 (Thermo Fisher Sci.) and compared to a “lo GC” negative control Stealth™ siRNA using JetPrimeR Polyplus-transfection as instructed by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: HEK-293T and HK-1 cells were maintained in Roswell Park Memorial Institute medium (RPMI 1640; Life Technologies) supplemented with 5% FCS (Sigma) ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK 293T/17 (CRL-11268) were obtained from ATCC and cultured in DMEM (high glucose, pyruvate; Gibco, 11995065), supplemented with 10% fetal bovine serum (Gemini bio-products ...
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bioRxiv - Synthetic Biology 2020Quote: All HEK 293FT cells were grown in DMEM supplemented with 10% FBS and 1% Antibiotic-Antimycotic (Thermo Fisher) in an incubator at 37 °C and 5% CO2 atmosphere ...
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bioRxiv - Synthetic Biology 2020Quote: All HEK 293FT cells were grown in DMEM supplemented with 10% FBS and 1% Antibiotic-Antimycotic (Thermo Fisher) in an incubator at 37 °C and 5% CO2 atmosphere ...
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bioRxiv - Neuroscience 2019Quote: HEK 293T cells were maintained in DMEM containing 10 % fetal bovine serum (HyClone) and 1 % L-glutamine (Invitrogen). Primary hippocampal or cortical neurons were prepared from E18 Sprague-Dawley rats (Orient Bio ...
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bioRxiv - Biochemistry 2020Quote: ... pastoris or HEK-293T cells was used to coat flat-bottom 96-well plates (Thermo Scientific NUNC-MaxiSorp) at a final concentration of 1 µg/ml (100 µl/well ...
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bioRxiv - Biochemistry 2020Quote: ... pastoris or HEK-293T cells was used to coat flat-bottom 96-well plates (Thermo Scientific NUNC-MaxiSorp) at a final concentration of 1 µg/ml (100 µl/well ...