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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then fixed and permeabilized using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher #00-5523-00) following manufacturer’s recommended procedure ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells then underwent fixation and permeabilization using an Biosciences Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher #00-5523-00) following manufacturer’s recommended procedure ...
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bioRxiv - Immunology 2023Quote: ... T cells were fixed and permeabilized using the eBioscience FoxP3/Transcription Factor Staining Buffer Set (Invitrogen, #00-5523-00) following manufacturer’s instructions and stained for intracellular cytokines for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... for 20 min at room temperature before fixation/permeabilization with the eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen) and intracellular staining for 40 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized using FoxP3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific Cat# 00-5523-00) and stained with anti-mouse antibodies targeting intracellular markers according to standard protocols.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with basic fibroblast growth factor (bFGF; 20 ng/ml; cat. no. 13256-029; Invitrogen; Thermo Fisher Scientific, Inc.), epidermal growth factor (20 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with basic fibroblast growth factor (bFGF; 20 ng/ml; cat. no. 13256-029; Invitrogen; Thermo Fisher Scientific, Inc.), epidermal growth factor (20 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Fixation was performed with 4% paraformaldehyde or Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific; 00-5523-00) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cell fixation and permeabilization was performed using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cell fixation and permeabilization was performed using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were permeabilized with 1X permeabilization buffer (PB) (kit Foxp3/Transcription Factor Staining Buffer kit, Thermo Fisher, 00-5523) for 30 min RT ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was performed using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen, Cat. 00-5523-00) at room temperature for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... the samples were fixed with eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent (Thermo Fisher Scientific, USA) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... the cells grown on glass coverslips covered with 1% Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Gibco), were fixed with 4% Pierce methanol-free formaldehyde (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was performed by fixing and permeabilizing with the eBioscience Foxp3 / Transcription Factor Staining Set (Thermo Fisher Scientific). EdU positive cells were stained with Click-iT Plus EdU AlexaFluor 488 Flow Cytometry Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed and permeabilized using the FOXP3/Transcription factor staining buffer kit (00-5523-00; Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/mL basic fibroblast growth factor (bFGF) (Prepotech, AF-100-18B) and 1x B27 (Thermo Fisher Scientific, 17504044). Cells were incubated at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized for 30 min with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) before staining with the following antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Complete media was prepared by supplementing with the following growth factors: 1.5% v/v Bovine Pituitary Extract (ThermoFisher Scientific) from 1mg/mL stock solution ...
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bioRxiv - Cancer Biology 2024Quote: ... and Foxp3 was performed using the eBioscience Foxp3 transcription factor fixation/permeabilization kit per manufacturer’s instructions (Thermo Fisher Scientific). Data were acquired on a FACS Symphony A5 (BD Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were washed with PBS and permeabilized using the Foxp3 Transcription Factor Staining Buffer Set (Invitrogen, 00-5523-00) for 1 h in the dark at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... NIM was exchanged daily for 3:1-DMEM/F12-medium (3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Cell Biology 2021Quote: ... 1–2 mg of VLDL were combined with 100 μL of 3 mg/mL 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD; Invitrogen) in DMSO and then re-isolated by ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... 50 nM siRNA (IMPDH2 assay ID: s7417, sense: 5’-CCAAGAAAAUCACUCUUtt-3’; anti-sense: 5’-UUAAGAGUGAUUUUCUUGGtc-3’, Ambion by Life technologies ...
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bioRxiv - Biochemistry 2023Quote: ... tRNAGln was 3’ biotin labeled with the Pierce ™ RNA 3’ end biotin labeling kit (Thermo Fisher) according to manufacturer’s recommendations ...
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bioRxiv - Biophysics 2022Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) and (ii) 1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) were both from Invitrogen. The standard lipids (i ...
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bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
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bioRxiv - Neuroscience 2024Quote: ... The siRNA sequence for YTHDF2 is 5′-AAGGACGTTCCCAATAGCCAA-3′ and for HIF1α is 5’-GCTGATTTGTGAACCCAT-3’ (Ambion). After 48 h from the initial transfection ...
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bioRxiv - Genetics 2022Quote: ... mcherry: 5’-ATGGTGAGCAAGGGCGAGGAG-3’ and 5’-CTTACTTGTACAGCTCGTCCATG-3’) from pKHR8-foxc1aKI and separately subcloned into pJET2.1 (ThermoFisher) to give pJET-mVenus and -mCherry ...
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bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with fresh ECGM and 3 times with ECGM/Hoechst 33342 (NucBlue™ ThermoFisher). After 10min incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM DTT) and homogenized using a Dounce homogenizer followed by DNaseІ treatment (3 U/µl, ThermoFisher) for one hour on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... UM-UC-3 cells were fluorescently labeled using 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI, ThermoFisher, #D3899) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... forward and reverse primers (800 nM; BLVpol_5f: 5′-CCTCAATTCCCTTTAAACTA-3′; BLVpol_3r: 5′-GTACCGGGAAGACTGGATTA-3′; Thermo Fisher Scientific), and 200 ng of gDNA template ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein quantification of the glass bead protein extracts was conducted using the BCA Protein Assay kit (Thermo Fisher), and samples with equal amount of proteins were applied on SDS-PAGE gels ...
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bioRxiv - Neuroscience 2020Quote: ... and pre-cleaned with protein A/G beads (Dynabeads™ Protein A, 10002D, Dynabeads™ Protein G, 10004D, Invitrogen). Immunoprecipitation was conducted with a mixture of Aβ antibodies (6E10 ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Microbiology 2021Quote: Purified ThsA protein was freshly prepared and protein concentration was quantified using Qubit Protein Assay Kit (ThermoFisher cat # Q33212). Protein was diluted in wash buffer to a concentration of 147 ng/μl ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the protein extract was then measured with a BCA Protein Assay Kit (Thermo Fisher Scientific). A volume of each protein extract corresponding to 14 mg protein was transferred into a new 5-mL tube containing 150 µL streptavidin beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Protein extracts from cells were assessed for protein concentrations using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Equal amounts of protein lysates were added to SDS-PAGE gels and electrophoresis was performed ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 500 ng ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 2 μg/μL with the IP lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: Protein concentrations were determined using the BCA protein assay (Micro BCATM protein assay kit; Thermo Fisher Scientific, MA, USA) and CLARIOstar microplate reader (BMG labtech Durham ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration was quantified using the “Protein Quantification BCA Assay” kit (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were measured using the Bio-Rad Protein Assay Kit or BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts (25 μg or 10 μg ...
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bioRxiv - Neuroscience 2023Quote: The brains of P9-11 C57 mice were immersed and fixed for 3 days in cold 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide HCl (EDC, Thermo Fisher Scientific, in 0.1 M PB, pH 7.4) at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: Samples of salmonid CF and non-salmonid OF (2 µg of proteins for each sample) were subjected to a short migration on a NuPAGE system (Invitrogen, 3 minutes at 200 V-400 mA - 23 W) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were measured using a protein assay kit (Pierce™ BCA Protein Assay Kit, #23225, ThermoFisher Scientific, Cheshire, UK). Protein samples were resolved through 10% SDS-polyacrylamide gel and transferred to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing proteins was collected and the protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Scientific). Immunoblotting analysis was carried out as previously described.(8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...