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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPENTANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... in PBS + 3% NDS at RT for 1 h followed by Goat anti Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (DyLight 405, Invitrogen #PI35500) in PBS + 3% NDS at RT for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... we washed 5 times with PB 0.1 M and incubated them for 1 h at room temperature in secondary antibody goat anti-rabbit IgG (H+L) Alexa 647 (Invitrogen, A32733) diluted 1:1000 in PB 0.1 M containing 1% NGS ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-Mouse IgG (H+L) Alexa Fluor Plus 488 and goat anti-rabbit IgG (H+L) Alexa Fluor Plus 488 (1:200, Thermo Fisher). Finally ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies used were anti-rabbit H+L Alexa Fluor 568 and anti-mouse H+L Alexa Fluor 488 (1:500; Life Technologies). After IHC ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed three times with 1X PBT for 5 minutes each and then incubated for 1 h at room temperature with secondary antibody (goat anti-rabbit IgG (H+L) Alexa Flour 546 (Invitrogen, A11035) or goat anti-mouse IgG (H + L ...
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bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
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bioRxiv - Systems Biology 2019Quote: ... Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 647-conjugated goat anti-mouse IgG diluted in washing solution for 1 h (1:100, Thermo Fisher Scientific Inc.; A11008 and 1:500, Thermo Fisher Scientific Inc.; A21236). After washing ...
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bioRxiv - Cell Biology 2021Quote: ... cover slips were incubated for 1 h at room temperature with mouse anti-mouse Keratin 14 (LL02, Thermo Fisher, MA5-11599, 1:100 – 1:10), rabbit anti-mouse Keratin 5 (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... and TagRFP-T-VAPB(1-218)-eMagB or TagRFP-T-eMagB-PHOSBP (prey) and iRFP-P4C at a 3:2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
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bioRxiv - Cell Biology 2019Quote: ... RNA interference was done by transfecting 100 nM siRNA (TPD54 1: GUCCUACCUGUUACGCAAU, TPD54 2:CUCACGUUUGUAGAUGAAA, TPD54 3:CAUGUUAGCCCAUCAGAAU) with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine (PED-A1) and BODIPY™ FL C5 were purchased from Thermofisher scientific ...
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bioRxiv - Biochemistry 2021Quote: ... approximately 1 pmol injected onto a Acclaim PepMap 100 guard column (75 μm×2 cm C18, 3 μm particles, 100 Å; Thermo Scientific) and separated on a 50 cm PepMap RSLC C18 (75 μm×50 cm ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...
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bioRxiv - Bioengineering 2019Quote: ... medium was switched to a B27-based Retinal differentiation medium (BRDM) (DMEM/F12 (3:1) with 2% B27 (w/o vitamin A, ThermoFisher Scientific, USA), 1x NEAA and 1x AA) ...
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bioRxiv - Microbiology 2020Quote: ... were performed on an Ultimate 3000 RSLC nano instrument coupled to either a QExactive Plus (M#1, M#3) or an HF mass spectrometer (M#2; Thermo Fisher Scientific) as described previously.24 Tryptic peptides were trapped for 4 min on an Acclaim Pep Map 100 column (2 cm × 75 μm ...
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bioRxiv - Physiology 2023Quote: ... IALVs were then washed with PBS containing 0.1% Triton X-100 (PBST) 3 times and blocked for a minimum of 2 hr with Blockaid® (B-10710, ThermoFisher Scientific). IALVs were then stained with the corresponding primary antibodies in BlockAid® Solution ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Physiology 2024Quote: ... Samples were washed 3 times with 0.025% PBS-Tween20 and incubated with Alexa-conjugated secondary antibodies listed in Supplemental Table 2 (Life Technologies, 1/1000e) for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: Transfection mix was prepared by combining PEI and plasmid DNA (4:1 ratio; 4 µg PEI per 1 µg DNA) in Opti-MEM™ Reduced Serum Medium (Thermo Fisher Scientific) followed by brief vortexing ...
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bioRxiv - Cell Biology 2021Quote: ... and/or CellTracker™ Blue CMAC (100 μM, 7-amino-4-chloromethylcoumarin, ThermoFisher Scientific). Stained cultures were re-normalized to equal cell numbers (0.5 OD600/mL) ...
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bioRxiv - Genomics 2021Quote: ... After adding the RT mix (7 µl H2O, 4 µl 5x RT buffer [Invitrogen] ...
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bioRxiv - Biochemistry 2023Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (ThermoFisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 230C ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were blocked for 1 hour in 2 mg ml−1 BSA and 1% Goat Serum (Thermo Fisher) in 1X PBSTXD (1X PBS ...
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bioRxiv - Immunology 2024Quote: ... CD206-FITC (19.2, 1:100), CD11c-PE (3.9, 1:50) and CD14-PerCP-Cy5.5 (61D3, 1:200) from eBiosciences (ThermoFisher) were used.
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated for 2 h with secondary antibodies conjugated to Alexa-488 (1:2000; Molecular Probes, Thermo Fisher Scientific, Waltham, MA, USA). For the D7F7 antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 2 hours at room temperature with goat anti-chicken IgY (H+L) Alexa Fluor™ 488 (Invitrogen, A-11039, 1:1000) diluted in TBS with 10% Serum ...
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bioRxiv - Immunology 2024Quote: ... incubated at room temperature for 2 hours with Alexa Fluor® 647-conjugated donkey anti-mouse IgG (H+L) antibodies (1:500, Invitrogen, # A-31571, RRID: AB_162542) and 0.8 µM Hoechst 33342 at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with following primary antibodies and/or labelling agents: anti-GFP (Invitrogen, #11122, 1:800, 3 days or Invitrogen, #10262, 1:800, 3 days), anti-V5 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was resuspended 3 times using 7 mL Wheaton Dounce tissue grinder (Fisher Scientific) and centrifuged at 12,000 rpm at 4°C for 30 minutes (Sorvall SS-34 centrifuge rotor) ...
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bioRxiv - Genetics 2020Quote: ... 25 μL of media containing STS and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at final treatment concentrations of 1 μM and 2.5 μM ...
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bioRxiv - Neuroscience 2022Quote: ... The electrodes were labelled with DiIC18(7) (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotricarbocyanine Iodide) (DiR) (Invitrogen) to enable post-mortem reconstruction of the electrode tracks in histological sections.
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bioRxiv - Immunology 2022Quote: ... Alexa Fluor 488 and caspase-3/7 activity detection dyes were purchased from Thermo Fisher, and Viobility™ 405/452 Fixable Dye from Miltenyi Biotec.
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bioRxiv - Microbiology 2023Quote: ... Apoptosis assays were perfomed using CellEvent Caspase 3/7 Green Detection Reagent (Thermo Fisher Scientific) and Live/Dead eFluor 660 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with the following secondary antibodies for 1 h: Alexa 546-conjugated goat anti-rabbit IgG (1:1,000, Thermo Fisher Scientific Cat# A-11010 ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells were washed 3 times in PBS and incubated for 1 h in the dark with the following secondary antibodies: Alexa Fluor 594 (1:200, Cat# A11037, Invitrogen) or Alexa Fluor 488 (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... reverse-transcribed at 50°C for 1 h ourwith SuperScript III and 1 mM of each dNTP (SuperScript III cDNA synthesis kit, Invitrogen) followed by 18-cycle PCR amplification.
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bioRxiv - Bioengineering 2019Quote: ... in 0.1% BSA in PBS) for 1 h, 37 °C, and then DAPI (1:1000, in 0.1% BSA in PBS) (Invitrogen, USA) was used for 5 min at room temperature to stain the nuclei ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated for 1 h at room temperature with Alexa 555-conjugated anti-rabbit IgG (1:100 dilution; ThermoFisher) in Can Get Signal immunostain (solution A) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 1 h at RT with the corresponding goat anti-rabbit Alexa Fluor 488 (1:250, ThermoFisher), donkey anti-rabbit Alexa Fluor 647 (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed with PBST before 1 h incubation at room temperature with a secondary antibody conjugated to Alexa Fluor 488 (1:500; Invitrogen). After several washes ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were then incubated for 1 h in Alexa 488 goat anti-chicken antibody (Life Technologies, #A11039; 1:500) in TBS containing 0.2% Triton X-100 and 2% normal goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 h at room temperature with second antibodies conjugated to Alexa dyes (1:1000, Molecular Probes) and washed again ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h in 0.1 M PBS-Tx 0.2% containing 10% normal goat serum (1:100 ThermoFisher, Melbourne, Australia) to block non-specific binding ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were incubated for 1 h at RT in darkness with the secondary antibodies (1/200; Alexa 488, Alexa 568 and Alexa 647; Invitrogen) and DAPI (4’,6-diamino-2-phenylindole ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate is then clarified by centrifugation at 10,000 rev min−1 for 1 h using a Sorvall centrifuge (Thermo Scientific) followed by filtration via 0.45µm syringe filters ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with PBS and incubated for 1 h at room temperature with AlexaFluor antibodies (Invitrogen, dilution 1:300) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... for 1 h at 37°C and then diluted 1:20 in 50 μg/mL yeast tRNA (cat #Am7119, Invitrogen). A known concentration of plasmid DNA used to prepare the virus was also diluted 1:200 in 50 μg/mL yeast tRNA and processed in parallel ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was then clarified by centrifugation at 10,000 rev min−1 for 1 h using a Sorvall centrifuge (Thermo Scientific) followed by filtration via 0.45μm syringe filters ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 1 h at RT with Cy5-conjugated goat anti-rabbit antibody (1:250, #A10523, Thermo Fisher Scientific) diluted in blocking buffer ...