Labshake search
Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Very Low Density Lipoprotein Receptor VLDLR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The isolated primary mouse hepatocytes were plated onto collagen-coated 6-well plates at a density of 500,000 cells/well in 4 ml of DMEM (Thermo Fisher Scientific) plus 10% fetal bovine serum ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were plated at a density of 1.5 × 10^5 per well on 6-well plates in Neurobasal media (Gibco 21103-049) containing B27 supplement (Gibco 17504-044) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Sf21 cells were grown to a density of 2 × 106 cells/ml at 27 °C in Sf900II medium (Thermo Fisher Scientific), infected with the V1 UNK stock of baculovirus ...
-
bioRxiv - Developmental Biology 2024Quote: mESCs were plated on gelatin-coated plates at a final density of 1 × 104 cells/cm2 in N2B27 medium (1:1 mix of DMEM/F12 (Gibco, 21331020) and Neurobasal medium (Gibco ...
-
bioRxiv - Immunology 2024Quote: ... ‘protein’ fractions were isolated by NaBr density gradient ultracentrifugation using CS-150GXII Micro-Ultracentrifuge (Hitachi) with S140-AT Rotor (Thermo Scientific) according to the available protocol for S140-AT rotor with modifications ...
-
bioRxiv - Immunology 2024Quote: Astrocytes were resuspended at a density of 6.11 × 106/mL in a buffer composed of 1% (vol/vol) Pluronic F-68 (Thermo Fisher, #24040032) and 18% (vol/vol ...
-
bioRxiv - Physiology 2024Quote: ... The optical density of the reaction product was read at 450 nm using the Multiskan FC microplate reader (Thermo Scientific, USA) after the initial incubation period (3 min ...
-
bioRxiv - Biophysics 2024Quote: ... VivoTrax-labeled NK-92 cells were centrifuged onto Cytospin glass slides at a density of 1 x 105 cells (Cytospin 4 centrifuge, Thermo Scientific). Attached cells were then fixed by methanol/acetic acid solution (3:1 ...
-
bioRxiv - Immunology 2023Quote: ... DCs and CD4+ T cells were washed and resuspended in RPMI supplemented with 2% FCS at a cell density of 1×107 cells/ml and labelled with 7.5μM Cell Tracker Red CMTPX (Thermofisher; Waltham, MA, USA) or 7.5μM vibrant CFDA SE (CFSE ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting pellet was resuspended in DMEM/F-12 and cell density was determined using the Countess 3 Automated Cell Counter (Invitrogen, AMQAF2000) using Trypan blue solution (Gibco ...
-
bioRxiv - Neuroscience 2023Quote: eGFP/Luc human iPSC-NSCs were seeded at a density of 1 x 104 cells per well in a Geltrex (Life Technologies) coated 96-well plate (flat bottom ...
-
bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were plated in T75 flasks at a density of 60-80% in a culture medium of high-glucose DMEM (Gibco 11965092) supplemented with 10% fetal bovine serum (Gibco 16140089 ...
-
bioRxiv - Microbiology 2024Quote: ... The DNA quantity and quality were estimated by measuring the optical density at A260/280 using the Nanodrop spectrophotometer (Thermo Scientific) and the Qubit™ 4 Fluorometer (Thermo Scientific).
-
bioRxiv - Bioengineering 2024Quote: The cell density for all samples was adjusted to 1 × 106 cells/mL in DMEM/F12 medium (Thermo Fisher Scientific Inc.) with 10% FBS and 10 μM Y27632 (FUJIFILM Wako Pure Chemical Corporation ...
-
bioRxiv - Cancer Biology 2024Quote: ... neurons were plated at a density of 0.8x106 cells per well of 6-well plates coated with 50 ug/mL Poly-D-Lysine (Thermofisher, #A38904-01) and 1ug/mL Laminin (Sigma ...
-
bioRxiv - Developmental Biology 2024Quote: ... at a density of 9×104 cells ml−1 in N2B27 differentiation medium containing Advanced DMEMF12 and Neurobasal media (1:1, Life Technologies), B27 w/o vitamin A (Life Technologies) ...
-
bioRxiv - Cell Biology 2024Quote: ... HEK-293T cells were seeded at a density of 750,000 cells/mL per 175 cm2 flask in 20 mL DMEM (Thermo Fisher Scientific) supplemented with 10% FBS (GeminiBio #100-106 ...
-
bioRxiv - Biochemistry 2024Quote: ... Corresponding protein-transfected cells were added and reseeded at a density of 2 x 105 cells/mL after trypsinization and resuspending in Opti-MEM without phenol red (Life Technologies). The system was allowed to equilibrate for 2 h at 37 °C/5% CO2 prior to BRET measurements ...
-
bioRxiv - Biochemistry 2024Quote: ... ExpiCHO-S cells were grown to a density of 6 x 106 cells/mL and transfected using the ExpiFectamine CHO Transfection Kit (ThermoFisher Scientific) and expression carried out for 7-10 days post-transfection at 37°C with 8% CO2 ...
-
bioRxiv - Biochemistry 2024Quote: ... Expi293F cells were grown to a density of 3 x 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and expression carried out for 3 to 5 days post-transfection at 37°C with 8% CO2 ...
-
bioRxiv - Immunology 2024Quote: ... followed by an FBS cushion density gradient (5mL of RPMI 1640 containing enriched APCs layered on 5mL of heat-inactivated FBS (Invitrogen, 12483020), 1000RPM for 10min at 4LJ ...
-
bioRxiv - Microbiology 2020Quote: ... Whole lungs were incubated overnight at room temperature in 25% isotonic Lugol’s solution w/v for contrast then in 0.75 % low melting point agarose (LMA, 16520050, ThermoFisher Scientific) w/v in 200 mM HEPEs incubated at 37°C for 1h ...
-
bioRxiv - Developmental Biology 2021Quote: ... Embryos were suspended in a solution of DMEM without phenol red containing 12.5% filtered rat serum and 1% low-melt agarose (Invitrogen) in a glass capillary tube ...
-
bioRxiv - Cell Biology 2020Quote: ... We cultured cells in customized media consisting of low-glucose (1 g/L) DMEM with phenol red (Gibco, USA), 1 g/L sodium bicarbonate ...
-
bioRxiv - Cell Biology 2020Quote: The HaCaT cell line (Human adult low Calcium high Temperature) were grown in Dulbecco’s Modified Eagle Media (DMEM, Invitrogen) supplemented with 2 mM essential amino acids (Invitrogen) ...
-
bioRxiv - Cell Biology 2020Quote: ... the growth media was replaced with imaging media: low glucose (1g/L) DMEM without phenol red (#11054020, Thermo Fisher), supplemented with 10% FBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... embryos were imaged with a 10 or 20x objective on a Zeiss LSM 700 microscope after mounting in 0.8-1% low melting point agarose (Invitrogen) on a glass bottom dish ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Real-Time PCR was performed with ABsolute Blue QPCR Mix low ROX 1X (Thermo Fisher Scientific, Waltham, MA, USA) and 2.5 µL of cDNA under the following thermal conditions ...
-
bioRxiv - Developmental Biology 2021Quote: ... decapsulated and transferred in low-binding Eppendorf tube containing 100-150µL collagenase solution in HBSS (Collagenase type IV (Gibco), 2X AAs (Gibco) ...
-
bioRxiv - Pathology 2022Quote: ... and re-suspended (500µL per kidney) in low salt buffer (20mM HEPES-KOH, Sigma Aldrich #H0527, 0,15M KCl, Thermofisher #AM9640G ...
-
bioRxiv - Bioengineering 2022Quote: ... We then gel-purified the final PCR products using a 2% low-melting point agarose gel (ThermoFisher Scientific, 16520050) and recovered the 210 bp band.
-
bioRxiv - Bioengineering 2022Quote: ... We then gel-purified the final PCR products using a 2% low-melting point agarose gel (ThermoFisher Scientific, 16520050). Pool testing enrichment was identically to library enrichment ...
-
bioRxiv - Cell Biology 2022Quote: ... Fraction V—Low-Endotoxin Grade (Gemini Bio 700-102P) was mixed in 10 mL of L-15 media (Gibco), to prepare a 1% BSA solution ...
-
bioRxiv - Genomics 2019Quote: ... we transduced cells with a RfxCas13d-expressing lentivirus at low MOI (<0.1) and selected with 5μg/mL Blasticidin S (ThermoFisher A1113903). Single cell colonies were picked after by sparse plating ...
-
bioRxiv - Genomics 2019Quote: For the GFP tiling screen RfxCas13d-expressing cells were transduced with pLentiEGFPdestabilized lentivirus at low MOI (<0.1) and selected with 100μg/ml Hygromycin B (ThermoFisher 10687010) for 2 days ...
-
bioRxiv - Developmental Biology 2019Quote: Each cuttlefish egg was then transferred to a 50 mm diameter petri dish that was coated with a ∼ 5mm layer of 0.5% low melting point agarose (16520050, ThermoFisher), and filled with culture medium (components described below) ...
-
bioRxiv - Microbiology 2019Quote: ... The PCR products were verified and quantified by agarose gel electrophoresis with a standard low DNA mass ladder (Invitrogen). Purification and OneShot Sanger sequencing of 16S rRNA gene products was performed by Genoscreen (Lille ...
-
bioRxiv - Cancer Biology 2020Quote: ... VCaP cells and C4-2B cells were grown for 24 hours in medium without L-glutamine supplemented with 1% ITS and 2.00 mM or low glutamine (0.02) (Life Technologies). When used ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR products were verified and quantified by agarose gel electrophoresis with a standard low DNA mass ladder (Invitrogen). Purification and OneShot Sanger sequencing of merA and merAB gene products was performed by Genoscreen (Lille ...
-
bioRxiv - Cell Biology 2020Quote: 3 dpf larvae were mounted on 25 mm #1.5 coverslips in an open bath chamber (Warner RC-40LP) and immobilized in E3 + Tricaine containing 1.2% low-melt agarose (Invitrogen). Agarose was removed from above the larvae with a #11 scalpel ...
-
bioRxiv - Bioengineering 2020Quote: ... at passage 17 were seeded at 420,000 cells/mL on fibrin and fibrinogen threads in low glucose DMEM with 10% fetal bovine serum (GIBCO). After 72 hours culture ...
-
bioRxiv - Cell Biology 2021Quote: ... CRL-1458) were cultured in low glucose (1.0 mg/mL) DMEM supplemented with antibiotics and 10% fetal bovine serum (Gibco). For transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... 100 PU.1 low and PU.1 high SLAM cells per well were sorted directly into 5µL of 2X Reaction Mix (Invitrogen) contained within a 96-well PCR plate ...
-
bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
-
bioRxiv - Cell Biology 2021Quote: ... CRL-1458) were cultured in low glucose (1.0 mg/mL) DMEM supplemented with antibiotics and 10% fetal bovine serum (Gibco). For transfection ...
-
bioRxiv - Microbiology 2021Quote: ... was operating at 200kV and images were acquired under low-dose conditions using the software EPU (Thermo Fisher Scientific) and a direct detector Falcon II (Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2021Quote: ... A large craniotomy was performed above the two OBs and covered with 1% low melting point agarose (Thermo Fisher). While the mouse was still anesthetized ...
-
bioRxiv - Cell Biology 2021Quote: RiboRad6mix and RiboRad6IP micrographs were recorded under low-dose conditions on a FEI Titan Krios electron microscope (ThermoFisher Scientific) equipped with a K3 direct electron detector using the Latitude software (Gatan ...
-
bioRxiv - Developmental Biology 2022Quote: ... 5,000 control or 125,000 mitomycin C treated cells were suspended in a 37 °C warmed 20 µL 0.5% low melting point agarose (16520, Invitrogen) droplet at the bottom of the µ-slide 8 well dish ...
-
bioRxiv - Developmental Biology 2022Quote: ... embryos at the proper stage were anesthetized as usual and embedded in 1.5% low melting agarose (ThermoFisher Scientific R0801) in E3 medium.