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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... HEK293F suspension cells were cultured in 293 Freestyle media (Gibco BRL Life Technologies) and cultured in a shaking incubator at 37°C ...
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bioRxiv - Microbiology 2022Quote: A stable Flp-In™ T-REx™ 293 cell line (Thermo Fisher) expressing the prefusion-stabilized HexaPro variant of the Wu Spike 68 was generated according to the manufacturer’s protocol (polyclonal selection) ...
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bioRxiv - Immunology 2024Quote: ... Pseudo-lentiviral particles were transduced into the FreeStyle 293-F cells (ThermoFisher Scientific). The stable 293-F cell line was established under 1.0 µg/ml puromycin selection ...
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bioRxiv - Immunology 2022Quote: ... HEK293F suspension cells were cultured in 293 Freestyle media (Gibco BRL Life Technologies) and cultured in a shaking incubator at 37°C ...
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bioRxiv - Immunology 2022Quote: ... HEK293F suspension cells were cultured in 293 Freestyle media (Gibco BRL Life Technologies) and cultured in a shaking incubator at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells (ATCC) cells had their media changed to FreeStyle 293 medium (Gibco) and transfected with the plasmids ...
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bioRxiv - Immunology 2024Quote: ... by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher, Cat. #A14524). Supernatants were harvested 4-5 days post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The T-REx™-293 cell line was obtained from Invitrogen (CA, U.S.A). T75 and T175 mammalian cell culture flasks were purchased from Fisher scientific (Loughborough ...
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bioRxiv - Immunology 2024Quote: ... were produced by co-transfection of Freestyle 293-F cells (Thermo Fisher Scientific) using the polyethyleneimine (PEI)-based precipitation method as previously described (58) ...
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bioRxiv - Immunology 2022Quote: Expi-293F cells were purchased from Thermo Fisher and maintained in Freestyle293/Expi-293 media (2:1, v/v, Thermo Fisher) in polycarbonate shaking flasks (Triforest Labware) ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI− cells were grown at 37°C in Freestyle 293 medium(Invitrogen) supplemented with 1.5% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the HEK293-derived cells Flp-InTM T-RexTM 293 Cell Line (ThermoFisher Scientific) were stably transfected to express HA-TDP-43 upon induction with tetracycline (1 μg/mL) ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were transiently co-transfected in FreeStyle 293-F cells using 293Fectin (ThermoFisher). All cells were tested monthly for mycoplasma ...
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bioRxiv - Biochemistry 2023Quote: Gibco Freestyle 293-F cell line (Thermo Fisher Scientific, USA; cat. no. R79007)
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bioRxiv - Cancer Biology 2023Quote: ... were transfected in 293 T cells with lipofectamine 3000 (Cat# L3000001, Thermo Fisher) to generate virus for transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... grown in FreeStyle™ 293 expression medium + 1% P/S (Thermo Fisher #12338018). One day prior to transfection ...
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bioRxiv - Biophysics 2024Quote: ... HEK293S GnTi- cells used for protein expression were cultured in Freestyle 293 (GIBCO) supplemented with 2% FBS and 1% Antibiotic-Antimycotic.
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bioRxiv - Microbiology 2024Quote: ... while FreeStyle 293F cells were cultured in FreeStyle 293 expression medium (Thermo Scientific) at 37°C with 8% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: Proximity labelling was performed in isogenic stable 293 Flip-In (Thermo Fisher; R75007) cells stably expressing TopBP1 N-terminal tagged with APEX2 or in 293T cells transiently expressing either N-terminal or C-terminally APEX2-tagged TopBP1 ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293T culture media was removed and replaced with FreeStyle 293 expression medium (Gibco) supplemented with 100 µg/mL streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... we used TaqMan probes for human HTT and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs obtained from Applied Biosystems. Relative HTT gene expression was determined using the ddCt method ...
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bioRxiv - Genomics 2019Quote: ... Primary antibodies (anti-human CD47-APC; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3; and clone B6H12.2, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 200 µg of human serum) were separated by SDS-PAGE on 3-8% tris-acetate gels (NuPAGE, Invitrogen) under reducing conditions at 150 V (cellular fraction ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 x 5 min with PBS and incubated with Alexa Fluor-488 goat anti human secondary (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in binding buffer containing a 1:100 dilution of mouse anti-human-IgG1-Fc conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL; A-10631) and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Synthetic Biology 2022Quote: ... while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel, Belgium). Ethanol (96% vol. ...
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bioRxiv - Microbiology 2019Quote: ... His-FruB or His-HPr in the cell extract was purified using HisPurTM-Ni-NTA Superflow Agarose (from Thermo Scientific, Cat # 25214) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
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bioRxiv - Genetics 2020Quote: ... pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy ...
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bioRxiv - Developmental Biology 2021Quote: ... and inserted into pMT/V5-His A (Thermo Scientific) using XhoI and SpeI target sites ...
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bioRxiv - Cell Biology 2022Quote: pAc5.1/V5-His vector (Thermo Fisher Scientific, Cat #V411020) was used to construct plasmids for expressing proteins or miRNAs in Drosophila S2 cells ...
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bioRxiv - Molecular Biology 2019Quote: ... and Ion PGM Hi-Q Sequencing Kit (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... with 10% heat-inactivated FBS (HI-FBS; Life Technologies)) ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Neuroscience 2020Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse anti-Penta-His-tag (#P-21315, ThermoFisher Scientific), 1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... and 9.9 μl of Hi-Di formamide (Applied Biosystems). These were then incubated for 5 min at 95°C and placed immediately on ice ...
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bioRxiv - Microbiology 2021Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or SARS-CoV-2 N-His (Sino Biological # 40588-V08B ...
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bioRxiv - Microbiology 2022Quote: ... or the mouse-anti-His (HIS.H8) primary antibody (Invitrogen) (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... and ligated into pcDNA3.1D/V5-His-TOPO vector (Invitrogen). Pseudoviruses were prepared by co-transfecting 1.25 µg of HIV-1 envelope containing plasmid with 2.5 µg of an envelope deficient HIV-1 backbone (PSG3Δenv ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
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bioRxiv - Neuroscience 2019Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
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bioRxiv - Biochemistry 2021Quote: ... Hi-5 cells (BTI-TN-5B1-4) (Gibco #B85502) were cultured in Express Five™ SFM (Serum-Free Media ...