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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Flp-In T-REx 293 cells were also supplemented with 100 μg/ml Zeocin (Invitrogen). Cells were passaged every 2-4 days ...
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bioRxiv - Immunology 2020Quote: ... Cells were maintained at 37°C and 6% CO2 FreeStyle 293 Expression Medium (Thermo Fisher) and 0.2% Penicillin/Streptomycin 7 days post transfection ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The transfection mix was replaced after 6 hours by 293 SFM II suspension medium (Invitrogen, 11686029 ...
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bioRxiv - Biochemistry 2023Quote: ... Baculovirus in the supernatant was pelleted and resuspended in FreeStyle 293 Expression Medium (Life Technologies) supplemented with 2% Fetal Bovine Serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: ... Expi293 cells were transfected with S-6P-NanoLuc expression plasmids using ExpiFectamine 293 (ThermoFisher Scientific). Four days later ...
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bioRxiv - Cell Biology 2023Quote: ... MCF7 (obtained from ATCC; cat# HTB-22) and 293 TREx cells (ThermoFisher, cat# R780-07) were cultured in Dulbecco’s modified eagle medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... All adenovirus vectors were produced in the T-REx-293 cell line (Thermo Fisher Scientific), which allows for transcriptional repression of the vaccine antigens during vector production ...
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bioRxiv - Immunology 2023Quote: ... All adenovirus vectors were produced in the T-REx-293 cell line (Thermo Fisher Scientific), which allows for transcriptional repression of the vaccine antigens during vector production ...
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bioRxiv - Bioengineering 2024Quote: ... according to the manufacturer’s instructions and cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific) in a humidified incubator with 8% CO2 at 37°C and 125 rpm ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Immunology 2022Quote: Recombinant soluble ICAM-1 protein was expressed in a Drosophila Melanogaster cells using pMT/V5-His vector with inducible promotor (Invitrogen), purified by sequential two-step affinity chromatography on anti-ICAM-1 antibody (clone YN1.1 ...
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2 μg His-tagged calcineurin was first bound to Ni-NTA-agarose or magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Physiology 2021Quote: ... The resulting cell pellet was resuspended in hypotonic buffer and used for Western blotting using HRP-conjugated anti-His antibody (1: 10,000; Novex, Life technologies).
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... and were maintained in DMEM high glucose (4500 mg/L) supplemented with 10% fetal bovine serum (FBS) (AlphaFBS-HI, Alphabioregen) and 1% Penicillin/Streptomycin antibiotics (Gibco), incubated at 37 °C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1% FBS (CPS Serum, Cat. No. FBS-500-HI) and 1X penicillin/streptomycin (Gibco, Cat. No. 15140-122), followed by centrifugation in an Eppendorf® centrifuge (model 5810 R ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-HAUS7 (OTI1E8, ThermoFisher, 1:1000 WB, 1:100 IF), mouse anti-acetylated tubulin (6-11-B1 ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal rabbit anti-mouse/human ZO-1 (1:200; Life Technologies), monoclonal rat anti-mouse ICAM-1 (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse ZO-1 (33-9100, Thermo Fisher Scientific, IF: 1/1000); rat ZO-1 (R40.76 ...
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bioRxiv - Bioengineering 2021Quote: ... 1:100 dilution of mouse anti-ZO-1 antibody (Life Technologies) and 3:200 dilution of AlexaFluor 488 ...
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bioRxiv - Cell Biology 2022Quote: ... goat anti-mouse IgG-HRP (1:10.000 - 1:50.000; 31430, ThermoFisher), goat anti-rabbit IgG-HRP (1:10.000 – 1:50.000 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse GSCs and mouse NSCs were fixed with 1% formaldehyde (Thermo Fisher scientific, 28906) for 10 min and quenched with 0.125 M glycine for 5 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Blots were developed with a mouse polyclonal anti-SmHDAC8 antibody (1/1000) or mouse polyclonal anti-SmRho1 antibody (1/1000) and peroxidase coupled anti-mouse secondary antibody (1/50 000; Invitrogen). Detection was carried out by chemiluminescence using SuperSignal™ West Dura Extended Duration Substrate (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... as then detected with mouse anti-myc (1:500) and visualized with donkey anti-mouse AF-488 (Invitrogen; 1:500). Nuclei were stained with propidium iodide (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... then placed in secondary antibody (goat anti-mouse IgG2a, Alexa 488, 1:1000, ThermoFisher; goat anti-mouse IgG1, Alexa 647, 1:1000, ThermoFisher) diluted in PBS with 2% horse serum and incubated for 2 hr at 22°C with rotation ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse HRP (Thermo Scientific #32430, 1:5000), anti-rabbit HRP (Thermo Scientific #31460 ...
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bioRxiv - Cell Biology 2020Quote: ... and mouse anti-Lamin B1 (1:1000, Invitrogen). The secondary antibodies used were donkey anti-rabbit or anti-mouse conjugated to IRDye 800CW or 680LT ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-CEA (1:100, Invitrogen, MA5-15070), mouse anti-Sox10 (1:800 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-ZO1 (IHC: 1:100; Thermo Scientific), mouse anti-PP2A (IB:1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Golgin97 (Molecular probes, # A21270, 1/200), rabbit anti-mCherry (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody anti-mouse AlexaFluor555 (1:500) (Invitrogen), or Alexa405 conjugated phalloidin (1:400 ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse IgG-HRP (1:20,000; Fisher Scientific) in combination with TMB (Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg/mL Natural Mouse Laminin (Life Technologies), 20 ng/mL BDNF (Peprotech #450-02) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg/mL Natural Mouse Laminin (Thermofisher, # 23017015), 10 ng/mL BDNF (R&D ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg/mL Natural Mouse Laminin (Thermofisher, # 23017015), 10 ng/mL BDNF (R&D ...
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bioRxiv - Developmental Biology 2021Quote: ... Alexa546-labelled anti-mouse (1:200; Life Technologies), Alexa647-labelled anti-rabbit (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% (v/v) normal mouse serum (10410, Invitrogen) and 0.5% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... donkey anti-mouse Alexa546 (1:500; Invitrogen A10036), donkey anti-rat Alexa488 (Invitrogen A21208) ...
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bioRxiv - Neuroscience 2022Quote: ... and donkey anti mouse-AF488 (ThermoFisher [1:500]). Sections were washed with 0.2% Tween and then with PBS only three times for 5 min at room temperature ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and 1:25000 Goat anti-Mouse (Invitrogen, 313430) secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa 555 goat anti-mouse (1:500; Invitrogen), Alexa 555 goat anti-rabbit (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa 633 goat anti-mouse (1:500; Invitrogen) and Alexa 633 goat anti-rabbit (1:500 ...