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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Siglec 10 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and then Fc receptors were blocked with CD16/CD32 antibody (Invitrogen). Cells were then surface stained with fluorochrome-labeled mAbs specific for mouse CD4 ...
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bioRxiv - Immunology 2021Quote: ... Lavage fluid was harvested and cells were Fc blocked (Invitrogen, Ireland) and stained on ice for 30 min (Table 3 ...
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bioRxiv - Immunology 2020Quote: ... and Fc blocked before being stained with CD45-eFluor450 (HI30; Invitrogen), Lineage cocktail 3-FITC (CD3 ...
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bioRxiv - Microbiology 2021Quote: ... with 7⍰5% heat-inactivated fetal calf serum (FCS; Life Technologies), at 37°C with 5% CO2 with 1% penicillin/streptomycin (PS ...
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bioRxiv - Microbiology 2022Quote: ... with 7 .5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... Optical density was measured at 405 nM (Multiskan−FC, Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... supplemented with 7.5% fetal calf serum (FCS; Gibco/ThermoFisher Scientific, 10270106) and 1% nonessential amino acids (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 7.5% fetal calf serum (FCS; Gibco/ThermoFisher Scientific, 10270106) and 1% nonessential amino acids (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: VHH72-Fc constructs were produced in ExpiCHO cells (Thermo Fisher Scientific) by transient transfection of the respective expression plasmids ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc (18-615) protein expressed in Expi293F (Life Technologies) cells was chemically biotinylated using EZ-link Sulfo-NHS-Biotin (A39256 ...
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bioRxiv - Immunology 2020Quote: ... and were used after freezing in Fetal Calf Serum (FCS) (Invitrogen) containing 10% DMSO (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... supplemented with 2% heat-inactivated FCS and 15 mM HEPES (Gibco). Intestines were cut open longitudinally ...
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bioRxiv - Bioengineering 2022Quote: ... for Fc-fusions and IgGs or Ni-NTA resin (Thermo Scientific) for Fab according to the manufacturer’s protocols.
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bioRxiv - Immunology 2023Quote: ... Optical densities were acquired with a Multiskan FC photometer (ThermoFisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... supplemented with 5% heat-inactivated fetal calf serum (FCS; Life Technologies), 1% penicillin/streptomycin (PS ...
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bioRxiv - Cell Biology 2024Quote: ... Measurements were done using Multiskan FC microplate photometer (Thermo Scientific, 51119000) and analyzed with SkanIt software version 3.1 (Thermo Scientific ...
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bioRxiv - Developmental Biology 2024Quote: The following antibodies were used: Fc Block (Invitrogen, 14-0161-86), live/dead eF780 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 5% fetal calf serum (FCS) (Gibco, Waltham, MA, USA), 0.5 ng/mL of recombinant human fibroblast grow factor-basic (FGF-basic ...
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bioRxiv - Microbiology 2023Quote: ... GlutaMAX™ (Thermo) supplemented with 5% fetal calf serum (FCS, Gibco) and 100 μg/ml penicillin-streptomycin ...
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bioRxiv - Plant Biology 2023Quote: ... and OD414 were measured with a Multiskan FC device (Thermo Scientific) in a 2-5 min interval for 1-2 hrs ...
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bioRxiv - Cancer Biology 2023Quote: ... without FCS supplemented with amphotericin B (1:166; 250μg/ml, Gibco), gentamicin (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... and 2% v/v Fc receptor binding inhibitor (Thermo Fisher Scientific)) ...
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bioRxiv - Immunology 2023Quote: ... KIR-Fc were conjugated to protein-A Alexa 647 (Invitrogen, #P21462) overnight at 1:4 (molar ...
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bioRxiv - Microbiology 2023Quote: ... containing 1:400 of Human BD Fc Block (ThermoFisher Scientific, BDB564219) per condition ...
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bioRxiv - Immunology 2023Quote: ... along with goat anti-human IgG Fc secondary antibody (polyclonal, ThermoFisher). HLA-ESC was detected with an anti-HLA-E antibody (clone 3D12 ...
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bioRxiv - Physiology 2023Quote: ... Optical densities were measured using FC Microplate Photometer (Thermo Fisher, USA) at 450 nm ...
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bioRxiv - Microbiology 2023Quote: ... monocytogenes OD620 by a Multiscan FC Microplate Reader (Thermo Scientific, Denmark). The threshold for growth was set at OD620 ≥ 0.08 ...
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bioRxiv - Developmental Biology 2023Quote: ... Feeder cell (FC) medium comprises Dulbecco’s modified essential medium (Gibco 41966052), 15% foetal bovine serum (Gibco 10270106) ...
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bioRxiv - Microbiology 2024Quote: ... Alexa Fluor 647 conjugated anti-human IgG Fc secondary antibodies (Invitrogen) (1:1500 ...
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bioRxiv - Cell Biology 2021Quote: ... cover slips were incubated for 1 h at room temperature with mouse anti-mouse Keratin 14 (LL02, Thermo Fisher, MA5-11599, 1:100 – 1:10), rabbit anti-mouse Keratin 5 (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Human PAR4 containing an N-terminal V5-epitope were stably expressed using the HEK293 Flp-In T-Rex cells according to the manufacturer’s protocol (Invitrogen). The concentration of tetracycline was titrated (700 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: For siRNA transfection experiments: HEK293 cells were grown in 6-well plates and RNAimax transfection reagent (Invitrogen #13778-150) was used following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were transfected with either NSUN6 wild-type and the miCLIP-mutant construct using Lipofectamine 2000 (Life Technologies) and harvested 24 hours later.
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bioRxiv - Molecular Biology 2020Quote: ... GFP-N was immunoprecipitated from two independent HEK293 cell pellets using 5μg of anti-GFP antibody (Life Technologies G10362). A total of 2% input material was obtained for size-matched control libraries (SMI ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (Invitrogen catalogue number R780-07). Cell cultures were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Genetics 2019Quote: HEK293 cells (CRL-1573, ATCC, VA, USA) were cultured in high-glucose DMEM (Cat. No. 11965092, Life Technologies, USA) supplemented with 10% FBS at 37 and 5% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... The HEK293 cells were transfected with 100 ng/well of pGL3-Fluc plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol at around 75-90% confluency in a 96 well plate ...
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bioRxiv - Biochemistry 2020Quote: ... Digested peptides from HEK293 and HeLa cells were respectively injected into LTQ-Orbitrap Velos and Q Exactive (Thermo Fisher) onto a 75 μm i.d ...
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bioRxiv - Microbiology 2020Quote: The full length CPSF6 protein was expressed in a suspension-adapted HEK293 cell line (Expi 293F, Thermo Fisher Scientific) by transfection of expression plasmid using ExpiFectamine 293 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney (HEK293) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L Glucose and GlutaMAX™ (Gibco) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with GFP constructs (250 ng/well) for 24 (HEK293) or 48 (SH-SY5Y) hours using Lipofectamine 3000 (0.75 µL/well; Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: HEK293 freestyle cells were transfected with this expression construct in combination with pBu-BirA using 293Fectin (Thermo fisher scientific) transfection reagent at 1μg DNA/10^6 cells ...
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bioRxiv - Neuroscience 2022Quote: ... which were used to directly transfect HEK293-Kir2.1 cells with the linear product using lipofectamine 3000 (Thermo Fisher Scientific). For patch clamp characterization in HEK293A cells ...
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bioRxiv - Developmental Biology 2022Quote: ... HEK293E or HEK293-6E cells were maintained in suspension cultures in Freestyle 293 Expression Media (Gibco, Waltham, MA, USA) supplemented with heat-inactivated fetal calf serum (1% ...
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bioRxiv - Molecular Biology 2019Quote: ... cell or nuclear lysates (400 ug) prepared from HEK293 or HDFs to Dynabeads Protein A (10001D, Thermo Fisher Scientific) that were prebound with 2–3μg of indicated antibody and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: Cells (primary neurons or HEK293 cells) were transfected with 1.6 µg/mL of a pIRES2-EGFP plasmid vector (Invitrogen) harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number ...
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bioRxiv - Immunology 2019Quote: ... we performed immunoprecipitation of CT-DNA stimulated HEK293 cells using the antibody to IRF3 pS386 crosslinked to Dynabeads (ThermoFisher) overnight at 4 C ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and Huh-7 cells were transfected with plasmid DNAs by standard calcium phosphate precipitation and Lipofectamine 3000 (Invitrogen), respectively.
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bioRxiv - Biochemistry 2021Quote: ... The construct with a N-terminal 8xHis-TwinStrep-GFP-tag were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in black 96 well plates with transparent bottoms ...