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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... were incubated with HA antibody (at a conc. of 1:100) pre-bound Protein-G Agarose bead (Invitrogen) or with pre-blocked anti-FLAG M2 beads and rotated overnight at 4□C ...
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bioRxiv - Genetics 2022Quote: ... Rad51 protein was detected by incubating the membranes with rabbit polyclonal antibody anti-Rad51 (1:2000, Thermo Fisher Scientific Cat# PA5-34905 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to nitrocellulose membranes and probed as follow: rabbit anti-GFP from Life Technologies (1:10,000), mouse anti-His from Qiagen (1:10,000) ...
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bioRxiv - Microbiology 2019Quote: ... the adherens junction protein E-cadherin was stained using an anti-E-cadherin HECD-1 monoclonal antibody (Invitrogen) at 1:100 with subsequent incubation with the secondary DyLight594 conjugated anti-mouse antibody (1:100) ...
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bioRxiv - Microbiology 2019Quote: ... 1 mL of fluorescent solution containing 0.5mL of 20x Nano Orange dye (Molecular Probes Protein Quantitation Kit N10271) and 0.5mL of FM4-64 dye (Molecular Probes FM4-64 ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin was pre-cleared for 1 hour with either protein A magnetic Dynabeads (Invitrogen, for KDM2A/B ChIP) or protein A agarose beads (Repligen ...
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bioRxiv - Cell Biology 2019Quote: β-galactosidase activity was analyzed in THP-1 cells and secreted proteins using the β-galactosidase assay kit (ThermoFisher) according to manufacturers instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted from infiltrated leaf patches in 1 ml 2×NuPAGE LDS sample buffer (Invitrogen, NP0008) containing 0.05mL/mL β-mercaptoethanol ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated for 1 h at 4°C with 40 µL of Protein AG UltraLink Resin (Thermo Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... limited proteolysis was performed by incubating proteins in a molar ratio of 200:1 with Trypsin-EDTA (Gibco) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... anti- HA-tag for KRAB protein (1:500, 32-6700 NOVEX HA epitope Tag antibody 5B1D10, Invitrogen, USA) and anti-b-Catenin (1:400 ...
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bioRxiv - Microbiology 2020Quote: Cell lysates were collected in 1% Triton X-100 buffer and quantified by Bradford protein assay (Thermo Scientific). Proteins were separated by SDS-PAGE and transferred to PVDF membrane (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1) polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Anti-SARS-CoV-2 Spike RBD rabbit polyclonal antibody was purchased from SinoBiological (Beijing ...
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bioRxiv - Immunology 2021Quote: ... B.1.351 or P.1 Spike protein were produced using HEK 293T cells transfected with Lipofectamine 3000 (ThermoFisher) using IgE-SARS-CoV-2 S plasmid variants (Genscript ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (RBD, 1:1,000, Invitrogen cat. no. PA5-114451). All primary antibodies were diluted in PBST + 5% milk and applied to membranes for ≥16 hours at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 24 μl of the protein sample was mixed with 1 μl of 20× SYBR orange dye (ThermoFisher Scientific). Each sample was prepared and measured in duplicates ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 mM MgCl2 containing 4.5 µM phosphate-binding protein labeled with a phosphate sensor (MDCC, Fisher Scientific) for intrinsic GTPase activity or containing 1 nM NF1-GAP for GAP mediated GTPase activity ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Genomics 2022Quote: PVDF membranes containing transferred proteins were incubated in blocking buffer (1 x TBS-T (Thermo Fisher Scientific, 28360), 5% Blotting-Grade Blocker (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellet was resuspended in 1 mL of reaction reagent (Pierce BCA protein assay kit; Thermo Scientific). The protein content was determined using a BCA protein assay according to manufacturer’s instructions with bovine serum albumin as the standard ...
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bioRxiv - Neuroscience 2022Quote: ... mouse brains and CV-1 fibroblasts were lysed using N-PER Neuronal Protein Extraction Reagent (ThermoFisher catalog # 87792). Samples were resolved by SDSPAGE using 10% acrylamide/bis-acrylamide gels and transferred to 0.2 μm pore size nitrocellulose (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... 1-10ul of acidified protein digest was loaded onto a 20mm × 75um Pepmap C18 trap column (Thermo Scientific) prior to elution via a 50cm × 75um EasySpray C18 column into a Lumos Tribrid Orbitrap mass spectrometer (Thermo Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... 2ug/mL of SARS CoV2 BA.1 RBD protein were coated on 96-well ELISA plates (Nunc Maxisorp) and left overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in polyclonal anti-RBPMS (RNA Binding Protein Multiple Partners; 1:1000; ThermoFisher, CAT: PA5-31231) at 4ºC ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was diluted 1:15 and estimation was done using BCA kit (Pierce, Life Technologies Corporation, CA, USA) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2 diluted whole cell extract in buffer C was added to Dynabeads Protein G (Thermo Fisher Scientific) pre-incubated with HEXIM1 antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was measured spectrophotometrically (ε280 21430 M-1cm-1, MW 24.79 kDa) using NanoDrop One (Thermo Scientific). Dialysed MSP1D1 was concentrated to 5 mg/ml using a 10 kDa MWCO Amicon Ultra centrifugal concentrator (Millipore) ...
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bioRxiv - Neuroscience 2023Quote: ... We then transferred the proteins to nitrocellulose membranes with pre-chilled NuPage Transfer Buffer (Life Technologies #NP0006-1) at 90V for 2 hours on ice.
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bioRxiv - Plant Biology 2023Quote: ... Immunoprecipitation was done using 1 µg of anti-GFP antibody (MBL) bound to Dynabeads protein A (Thermo Fisher). After washing and elution ...
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bioRxiv - Biochemistry 2023Quote: ... 24 μl of the protein sample was mixed with 1 μl of 20× SYBR orange dye (ThermoFisher Scientific). Each sample was prepared and measured in duplicates ...
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bioRxiv - Synthetic Biology 2023Quote: ... The SeeBlue Plus2 pre-stained protein standard was used in lane 1 and lane 10 (Thermo Fisher Scientific). Gels were rinsed three times in DI water and then soaked for 1 hr in SimplyBlue SafeStain (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... the Pgk1 protein was recognized with a primary mouse monoclonal anti-Pgk1 (1:5,000; Thermo Fisher Scientific, 22C5D8), the aid tag was recognized with a primary mouse monoclonal anti-miniaid (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... or with Protein A agarose beads incubated with 2 μg anti-LONP1 antibody (ThermoFisher Scientific 15440-1-AP) overnight (16 hours ...
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bioRxiv - Physiology 2022Quote: ... Protein abundance was normalized to total protein using Pierce™ Reversible Protein Stain Kit for PVDF Membranes (cat 24585; Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific; cat # 23227). Twenty or 30 µg samples were loaded into SDS-PAGE gels and transferred onto either Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Purity and integrity of all proteins were checked and protein concentrations estimated using quantitative densitometry on GelCode Blue-stained protein gels (Thermo Fisher Scientific) containing bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were kept and protein concentration was estimated using the micro-BCA Protein Assay Kit (Pierce BCA Protein Assay Kit (Thermo Scientific: 23225)) ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was measured using the Pierce 660nm Protein Assay Kit or the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Immunology 2020Quote: ... and human antibody (bound to phage) was immunoprecipitated using 40ul of a 1:1 mix of protein A/G magnetic beads (Thermo Fisher, Waltham, MA, #10008D & #10009D). Beads were washed 4 times and antibody-bound phage were eluted into 1ml of E ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA in the protein samples was sheared by sonication and the amount of protein was estimated using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were separated on 4–20% Mini-PROTEAN® TGX™ Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™, PI23227). The desirable amount of the protein lysate (15 – 30 μg ...
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bioRxiv - Microbiology 2022Quote: ... The EBOVLP input was normalized based on the concentration of EBOV structural proteins as determined with a bicinchoninic acid (BCA) protein assay (Pierce™ BCA protein assay kit, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014, respectively) for one hour ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified with a Micro BCA protein assay kit (Thermo Scientific, Rockford, IL) using the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein concentration was determined using the Bicinchoninic Acid protein assay (Thermo Scientific, US). Lysates (3μg/μl in 1xSDS ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was adjusted using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). For co-immunoprecipitation assay ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins separated by SDS-PAGE were stained with PageBlue Protein Staining Solution (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentrations were determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), before extracts were stored at −80°C ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were determined using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were estimated using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific# 23227). 400-500μg of protein from the stably transfected HEK293 cells were used to immunoprecipitate FLAG-tagged MIB1 using EZview™ Red ANTI-FLAG® M2 affinity gel (MilliporeSigma# F2426 ...