Labshake search
Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... 1:500) and biocytin filled cells (2⁰ Streptavidin-568 (Invitrogen #S11226) 1:500 ...
-
bioRxiv - Neuroscience 2024Quote: ... stained with 4,6-diamino-2-phenylindole (DAPI) (1:10,000; Invitrogen, D1306) in PBS for 10 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 1 µg/mL 4,6-diamidino-2-phenylindole (DAPI) (Invitrogen, #D1306) in PBS for 1 hour at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Larval zebrafish at 6-7 dpf were paralyzed by immersion in 1 mg/ml alpha-bungarotoxin solution (Invitrogen) dissolved in external solution (in mM ...
-
bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
-
bioRxiv - Cell Biology 2022Quote: ... INS-1 cells were transfected in 6-well plates with 5 nM siRNA using Lipofectamine RNAiMAX (Invitrogen, 13778030) and typically analyzed after 96 hours ...
-
bioRxiv - Molecular Biology 2019Quote: ... Aliquots were solubilized in 1 mL 8M urea lysis buffer: 8M Urea (Invitrogen, CA; CAS# 57-13-6), 100mM NaCl(Fisher Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... Desiccated samples were subjected to 5-6 rounds of desiccation and rehydration (1-hour speedvac desiccation [Thermo Fisher Scientific] followed by resuspension in water) ...
-
bioRxiv - Microbiology 2021Quote: ... L454W/E455G and S262R) and mCherry (6:4:1 mixtures; 2.2 μg/well) using Lipofectamine® 2000 (Invitrogen), according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2019Quote: ... Mice were euthanized by CO2 asphyxiation at 6 h and lungs placed in 1 ml TRIzol (Thermo Fisher). RNA was then extracted using acid-phenol-chloroform-isoamyl alcohol (125:21:1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were seeded in 6-well plates in 1 ml of RPMI 1640 medium (Gibco, Amarillo, TX, USA) supplemented with 10% fetal bovine serum and 0.2% primocin (Invivogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... HL-1 cells in 6-well plates were transfected with 15 µL Lipofectamine 2000 reagent (Life Technologies 11668019) complexes with nucleic acid ...
-
bioRxiv - Neuroscience 2020Quote: Neocortex from C57Bl/6 pups (postnatal day 1-3) was dissected in Hibernate-A medium (#A1247501, Gibco, USA), digested with papain (#LS003124 ...
-
bioRxiv - Genomics 2020Quote: ... the cells were split 1:6 with Accutase (StemPro® Accutase® Cell Dissociation Reagent, Gibco™ A1110501), onto matrigel substrate in the presence of 10 μM of rock inhibitor (Y-27632 dihydrochloride ...
-
bioRxiv - Genomics 2021Quote: ... hiPSC-IMR90-1 cells were cultured on a Matrigel-coated 6-well plate with Essential 8 medium (Gibco). The medium was changed daily ...
-
bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
-
bioRxiv - Cell Biology 2021Quote: ... and selected antibiotics according to the respective expression constructs: blasticidin S (6 μg mL−1, Thermo Fisher Scientific), puromycin (0.5 μg mL−1 ...
-
bioRxiv - Immunology 2022Quote: ... rested for 6 hours and stimulated overnight in 1 ml of complete medium (RPMI 1640 with Glutamax, Gibco) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 0.5:1:0.1 (4 μg/6-well dishes) using Lipofectamine LTX PLUS (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were next treated with 1: 1000 dilution of primary antibody (6-His tag mouse anti-tag, Invitrogen) for 1 hr at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... coated 6-well plate with 1x mTeSRTM-1 medium and passaged with TrypLE Express (Gibco by Life Technologies) and plated in medium containing 3uM Rho-kinase inhibitor Y-27632 (Abcam ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were then washed 6× and 50 µl of 1-Step™ 3,3’,5,5’-tetramethylbenzidine (TMB; Thermo Fisher) substrate was added to each well and incubated for 3 min ...
-
bioRxiv - Cell Biology 2023Quote: ... HeLa SMC4-AID_WAPL-dTAG cells were supplemented with blasticidin S (6 µg mL-1, Thermo Fisher Scientific, A1113903) and hygromycin B (0.3 mg mL-1 ...
-
bioRxiv - Genomics 2023Quote: ... Cells were then co-transfected with the two plasmids at a molar ratio of 6:1 (2.5 μg of homology vector, ∼0.7 μg of sgRNA vector) using Lipofectamine2000 (ThermoFisher). The next day ...
-
bioRxiv - Neuroscience 2023Quote: ... coated 6-well plate with 1x mTeSRTM-1 medium and passaged with TrypLE Express (Gibco by Life Technologies) and plated in medium containing 3uM Rho-kinase inhibitor Y-27632 (Abcam ...
-
bioRxiv - Cell Biology 2024Quote: ... we used 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH; Thermofisher Scientific Cat. No. T204). Yeast cells were stained with 0.5µM TMA-DPH ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
-
bioRxiv - Microbiology 2024Quote: ... Beads were washed 6 times with 1 mL each of ChIP buffer on a magnetic tube rack (Invitrogen). After removing the final wash ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells nuclei were counter-stained using 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) mounting medium (ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher Scientific, Warsaw, Poland). Preparations were observed using the confocal microscope under magnification 630× and processed with Fiji software (ImageJ 1.52n ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
-
bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Several donor colonies and one recipient colony were independently inoculated in 2 mL of LB in 15-mL tubes and cultured for 6 hours (37°C and 250 rpm, Thermo Scientific™ MaxQ™ 8000). Cultures were centrifuged (15 minutes ...
-
bioRxiv - Cancer Biology 2019Quote: ... was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). In vitro experiments with KJON cells were performed with 2 % HS in RPMI and IL-6 (1 ng/mL) ...
-
bioRxiv - Biochemistry 2021Quote: ... Enzyme assays were conducted in 0.5 mL containing 50 mM 2-(N-morpholino)ethanesulfonic acid (pH 6) and various concentrations of soluble starch (Acros Organics, No. AC424495000, Morris Plains, NJ). The assays also included 100 mM KCl ...
-
bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
-
bioRxiv - Genomics 2021Quote: ... The embryoid medium was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Antibiotic-Antimycotic (Thermo Fisher Scientific; Cat. No. 15240062), 55 mM 2-mercaptoethanol (BioRad ...
-
bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
-
bioRxiv - Neuroscience 2024Quote: ... then separated on a reverse-phase nano-spray column (2 μm, 75 mm i.d. × 50 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) using gradient elution ...
-
bioRxiv - Immunology 2021Quote: ... 1 × 105 activated NKT cells were incubated with 1 mM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Invitrogen) in RPMI complete media for 30 minutes at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... 20 μl RNase A/T1 mix (2 μg μl−1 / 5 U μl−1, Thermo Scientific) was added to 100 μl lysate (OD260 ~ 150 ...
-
bioRxiv - Cancer Biology 2019Quote: ... 1×106 cells were transfected with 1:2 ratio of plasmid and Lipofectamine 3000 (Thermo Scientific). The protein or mRNA were isolated after 36-48 hours of transfection ...
-
bioRxiv - Cancer Biology 2022Quote: ... in a 1:1 ratio and maintained in R10 + IL-2 [RPMI 1640 (Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: ... and switching to SLI medium (Advanced DMEM/F12 (1:1) supplemented with 2 mM GlutamaxTM (Gibco), 2% B27 without vitamin A (Life technologies) ...
-
bioRxiv - Cancer Biology 2020Quote: ... were purchased from Click Chemistry Tools and Tris [(1-benzyl-1H-1, 2, 3-triazol-4-yl)methyl] amine from Fisher Scientific.
-
bioRxiv - Bioengineering 2023Quote: ... Drug selection began 2 d post transduction with 1 μg/mL puromycin (Invitrogen, ant-pr-1). Cells were kept in antibiotics for at least two weeks with subculturing every one to two days before further characterization.
-
bioRxiv - Bioengineering 2024Quote: ... penicillin 100U.ml-1 /streptomycin 100 μg.ml-1 and L-glutamine 2 mM (Life Technologies, Inc., USA) in 5% CO2 humidified atmosphere at 37 °C.
-
bioRxiv - Cell Biology 2024Quote: ... and activated 1:1 with CD3/CD28 Dynabeads in 100 U/mL of IL-2 (Thermofisher). The next day ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Nvsix3/6:venus was generated by subcloning the Nvsix3/6 coding sequence into pENTR/D TOPO (ThermoFisher Scientific) using published primers previously used to PCR amplify Nvsix3/6 and synthesize Nvsix3/6 mRNA 47 ...
-
bioRxiv - Microbiology 2021Quote: ... thaliana seedlings cultured in 6 ml liquid MSgg of each well of a 6-well microplate (Thermo Scientific). 8 seedlings were put in each well ...
-
bioRxiv - Microbiology 2020Quote: ... Cells in antibiotic free media (8×105/6-well) were transfected 6 hours post infection with 2μg plasmid and 6μl Lipofectamine 2000 (Invitrogen) per well diluted in Opti-MEM (Invitrogen).