Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media citations for Thermo Fisher

Quote: ... slides were dehydrated through an ethanol series and incubated with 2 × SSC supplemented with 100 µg ml−1 RNase A (Invitrogen) at 37°C for 60 min ...

The cytoskeleton adaptor protein Sorbs1 controls the development of lymphatic and venous vessels in zebrafish

Alexandra Veloso, et al., bioRxiv - Developmental Biology 2020

Quote: ... Every time point was fixed with paraformaldehyde 4% overnight at 40C and then dehydrated and rehydrated through methanol and PBS 1x (Gibco)-Tween 5% washes ...

Transfer learning of an in vivo-derived senescence signature identifies conserved and tissue-specific senescence across species and diverse pathologies

Christopher Cherry, et al., bioRxiv - Bioengineering 2022

Quote: ... slides were dehydrated in ethanol and xylene and finally mounted using Permount mounting medium (Fisher Scientific, product number SP15-100).

Label-free Raman microspectroscopy for identifying virocells

Indra Monsees, et al., bioRxiv - Microbiology 2021

Quote: ... Afterwards the sample was again washed in 0.5 mL 1X PBS and dehydrated at room temperature in 50 vol% and 80 vol % ethanol (Fisher Scientific) for 10 min each ...

Quinomycin A reduces cyst progression in Polycystic Kidney Disease

Priyanka S Radadiya, et al., bioRxiv - Genetics 2020

Quote: ... Tissue sections for IHC were then dehydrated with graded ethanol and mounted with permount before coverslipping (Fisher Scientific, Pittsburg, PA). Slides were visualized and imaged using a Nikon 80i microscope with a photometrics camera or a Nikon Eclipse TiE attached to an A1R-SHR confocal ...

Culture system for longitudinal monitoring of bone remodeling ex vivo

E.E.A. Cramer, et al., bioRxiv - Bioengineering 2023

Quote: ... and subsequently dehydrated through graded ethanol series and xylene in an automatic tissue processor (Microm STP-120, Thermo Fisher Scientific) and embedded in paraffin (P3558 ...

Structure of mycobacterial respiratory Complex I

Yingke Liang, et al., bioRxiv - Biochemistry 2022

Quote: ... Excised bands were destained with 25 mM NH4HCO3 (pH 7.0) and 50% (v/v) acetonitrile and dehydrated in a refrigerated vacuum concentrator (Speedvac - Thermo Fisher). MS-grade trypsin/Lys-C mix (200 ng ...

Chemical modification of human decellularized extracellular matrix for incorporation into phototunable hybrid-hydrogel models of tissue fibrosis

Rukshika S. Hewawasam, et al., bioRxiv - Bioengineering 2022

Quote: ... Slides were dehydrated by sequential submersion in 100% ethanol (2× 5 min) and SafeClear II (2× 5 min, Fisher Scientific) before being mounted in Cytoseal 60 (ThermoFisher ...

Retinoid-impregnated nanoparticles enable control of bone growth by site-specific modulation of endochondral ossification in mice

Masatake Matsuoka, et al., bioRxiv - Pharmacology and Toxicology 2024

Quote: The hind limbs were fixed with 2% formaldehyde and dehydrated by increasing sucrose percentage to 30% and then embedded in the optimal cutting temperature (O.C.T.) compound (Thermo Fisher Scientific). Undecalcified frozen sections were mounted on the Kawamoto’s film and subjected to in situ hybridization using the RNAscope® Fluorescent Multiplex Kit (Advance Cell Diagnostic Inc ...

Androgen and glucocorticoid receptor direct distinct transcriptional programs by receptor-specific and shared DNA binding sites

Marina Borschiwer, et al., bioRxiv - Genomics 2020

Quote: ... LC-MS/MS analysis of the tryptic digests was performed on an Orbitrap Fusion Tribrid mass spectrometer equipped with a Proxeon nLC1000 system (Thermo Scientific) using the same settings ...

Evolution of a putative, host-derived endosymbiont division ring and symbiosis-induced proteome rearrangements in the trypanosomatid Angomonas deanei

Jorge Morales, et al., bioRxiv - Microbiology 2021

Quote: ... Peptides of tryptic digested samples were separated over 2h on C18 material using an Ultimate3000 rapid separation system (Thermo Fisher Scientific) as described61 and subsequently analyzed with an online coupled mass spectrometer in data dependent mode ...

Cyclophilin A regulates protein phase separation and mitigates haematopoietic stem cell aging

Laure Maneix, et al., bioRxiv - Cell Biology 2021

Quote: An aliquot of the tryptic digest was analysed by LC-MS/MS on an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex) ...

Proteomic characterization of pilot scale hot-water extracts from the industrial carrageenan red seaweed Eucheuma denticulatum

Simon Gregersen, et al., bioRxiv - Biochemistry 2020

Quote: Tryptic peptides were analyzed by an automated LC–ESI–MS/MS consisting of an EASY-nLC system (Thermo Scientific, Bremen, Germany) on-line coupled to a Q Exactive mass spectrometer (Thermo Scientific ...

Light-dependent THRUMIN1 phosphorylation regulates its association with actin filaments and 14-3-3 proteins

Matthew E. Dwyer, Roger P. Hangarter, bioRxiv - Cell Biology 2021

Quote: ... tryptic peptides were injected into an Easy-nLC 100 HPLC system coupled to an Orbitrap Fusion Lumosmass spectrometer (Thermo Fisher Scientific). Specifically ...

Tensin3 interaction with talin drives formation of fibronectin-associated fibrillar adhesions

Paul Atherton, et al., bioRxiv - Cell Biology 2021

Quote: Tryptic peptides were analysed using an EASY-nLC 1000 coupled to a Q Exac-tiveTM Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water at flowrate of 2 00nl/min ...

Epithelial protein lost in neoplasm (EPLIN)-β is a novel substrate of ornithine decarboxylase antizyme 1, mediating cellular migration

Dan Li, et al., bioRxiv - Cancer Biology 2022

Quote: Tryptic peptides were analysed using an EASY-nLC 1000 coupled to a Q Exactive™ Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50 cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water at a flow rate of 200nl/min ...

The Eucalyptus grandis chloroplast proteome: leaf development and seasonal variations

Amanda Cristina Baldassi, Tiago Santana Balbuena, bioRxiv - Plant Biology 2022

Quote: ... Tryptic peptides were resuspended in H2O containing 0.1% formic acid and separated in an EASY-nLC1000 chromatographic system (Thermo Fisher Scientific) for 95 minutes using a C18 nano-column under a constant flow of 400 nL/min and increasing concentrations of acetonitrile (7 to 30% over 70 min and then 70% in 25 min) ...

Biochemical and Immuno-Histochemical Localization of Type IIA Procollagen in Annulus Fibrosus of Mature Bovine Intervertebral Disc

Audrey McAlinden, et al., bioRxiv - Biochemistry 2021

Quote: ... Electrospray MS was performed on the tryptic peptides using an LTQ XL linear quadrapole ion-trap mass spectrometer equipped with in-line liquid chromatography (ThermoFisher Scientific) using a C8 capillary column (300 x150 mm ...

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