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Citations for Thermo Fisher :
1601 - 1650 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... coli Mach1 competent cells (Thermo Fisher Scientific) for plasmid amplification ...
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bioRxiv - Cell Biology 2024Quote: ... coli strain (Thermo Fisher Scientific, C3030-06) was routinely used as a cloning and plasmid amplification host ...
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bioRxiv - Microbiology 2024Quote: ... coli (Thermo Fisher Scientific, Wilmington, MA, USA) and purified as described by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... coli BL21 Star™ (DE3) cells (Invitrogen) in 500 mL of 2YT medium supplemented with 50 μg/mL kanamycin at 37°C and 160 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... coli LMG194 cells (Invitrogen, Carlsbad, CA, USA) were transformed with the pBAD plasmid containing the genes encoding SiRFP-60Δ ...
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bioRxiv - Microbiology 2024Quote: ... coli cells (Thermo Fisher Scientific, Waltham, MA), which were then grown in 500 ml of LB media supplemented with 100 µg/ml of ampicillin at 37⁰C inoculated from a starter culture ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 ng/ml recombinant mouse EGF and 100 ng/ml recombinant murine Noggin (all from Thermo Fisher Scientific). Organoids were passaged 3 times a week depending on culture confluence.
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bioRxiv - Evolutionary Biology 2021Quote: ... We selected libraries within the size range of 450-500 bp using a 2% E-gel on an E-Gel Electrophoresis System (Invitrogen, Canada), and re-amplified the size-selected libraries using a NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... the libraries were checked for correct fragment length on an E-Base device using E-Gel 96 Gels with 2% mSYBR Safe DNA Gel Stain (Fisher Scientific), quantified with a QuantiFluor dsDNA System (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... lectin PNA tagged with Alexa Fluor 488 (Thermo Fisher Scientific Inc.) was used.
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bioRxiv - Molecular Biology 2020Quote: ... GST-tagged NgAgo variants were purified by glutathione agarose (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 100 ng/mL pHrodo tagged zymosan A beads (Thermo Fisher Scientific), 100 ng/mL pHrodo tagged S ...
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bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were eluted by incubation with HA peptide (ThermoFisher) for 2 × 15 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: To localize GFP-tagged fusion proteins with BODIPY Red (Molecular Probes), transfected cells were grown overnight (16-20h ...
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bioRxiv - Cell Biology 2020Quote: ... To localize GFP-tagged fusion proteins with MitoTracker Red (Molecular Probes), after 16h transfection ...
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bioRxiv - Microbiology 2021Quote: ... for FLAG® (DYKDDDDK)-tagged proteins in iBind Western System (Invitrogen). Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2019Quote: ... tagged proteins were eluted and quantified by BCA (Pierce, ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... contained 40 µM human Flag-tagged ubiquitin (Fisher Scientific U-120), 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific, E304050), 2.4 µM human His6-UBE2T E2 enzyme (Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Myc-tagged proteins were immunoprecipitated with Protein G Dynabeads (Invitrogen, 10003D) coated with anti-myc antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated in Alexa tagged secondary antibodies (Life Technologies, Invitrogen) prepared in SG-PBS for 1hr at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated in Alexa tagged secondary antibodies (Life Technologies, Invitrogen) prepared in SG-PBS for 1hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Tagged proteins were visualized using chemiluminescent reagents (Thermo Fisher Scientific 34579).
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bioRxiv - Neuroscience 2023Quote: ... Sections were then stained with fluorescently tagged secondary antibodies (Thermo Fisher Scientific Cat# A10037 ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria are tagged with FM-4-64 dye (Thermo Scientific). In the confocal microscope study ...
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bioRxiv - Biophysics 2024Quote: ... Nitrocellulose membrane was incubated with His6-tagged Monoclonal Antibody - HRP (Invitrogen) with 1:3000 v/v dilution in PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... and AlexaFluor 488-tagged donkey-anti-mouse (Molecular Probes, 1:1000) secondary antibodies and Hoescht 33342 stain (Molecular Probes ...
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bioRxiv - Neuroscience 2023Quote: ... and AlexaFluor 647-tagged donkey-anti-rabbit (Molecular Probes, 1:1000) secondary antibodies for one hour ...
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bioRxiv - Cell Biology 2023Quote: ... The RFP-tagged organelle markers were purchased from Invitrogen (Carlsbad, CA): CellLight Golgi-RFP BacMam 2.0 (Cat# C10593) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3mM CaCl2 with 250 units of 6xHIS-tagged SUMO protease (ThermoFisher) for scar-free SUMO tag cleavage ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The tagged cDNA fragments were subcloned into expression vector pcDNA3.1(+) (Invitrogen). Transient transfection of HEK293 cells by plasmid DNA was carried out by Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/mL recombinant murine EGF (Gibco, PMG8043), 100ng/mL recombinant human Noggin (Peprotech ...
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bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human GDNF (Thermo Fisher), 10ng/mL recombinant human CNTF (Thermo Fisher)).
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bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human BDNF (Thermo Fisher), 10ng/mL recombinant human GDNF (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... 10ng/mL recombinant human CNTF (Thermo Fisher)).
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bioRxiv - Bioengineering 2021Quote: ... on recombinant human vitronectin (50μg/ml; Gibco) coated plates ...
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bioRxiv - Bioengineering 2020Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen), 0.178 mM Adenine (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/ml recombinant EGF (Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Immunology 2022Quote: ... The recombinant ORF8 (ThermoFisher, Cat. RP-87666) was conjugated with FITC using the FITC conjugation kit (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) was added to lysate (1ul per 100ul lysate) ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/ml human recombinant EGF (Invitrogen), 10 ng/ml bFGF (R&D Systems ...
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bioRxiv - Genomics 2019Quote: ... 0.25 μL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), was then added to each well ...
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bioRxiv - Cancer Biology 2020Quote: ... purified recombinant human IL-8 (ThermoFisher Scientific) and CXCL1 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant Cas9 2.0 (Thermo Fisher Scientific, A36498) was mixed with gRNA and incubated for 15 min at room temperature ...
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Differential expression of transposable elements in stem cell lineages of the preimplantation embryobioRxiv - Developmental Biology 2020Quote: ... 25 ng/mL human recombinant FGF4 (Gibco), and 1 μg/mL heparin (Sigma-Aldrich)] as previously described (Tanaka et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 0.15 ng of human recombinant VEGF (Gibco) were co-injected with 1.0×106 cells of WS-iMSC.
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bioRxiv - Neuroscience 2021Quote: ... Recombinant LRRK2 protein was from ThermoFisher (#A15197). For pT73 Rab10 assay ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant pSinRep5 and helper plasmid pDH26S (Invitrogen) were transcribed in vitro into capped RNA using the Megascript SP6 kit (Ambion) ...
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bioRxiv - Developmental Biology 2021Quote: ... G-CSF Recombinant Human Protein (Thermo fisher), IP injection ...