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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Protein Labeling kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: LNascent EU RNA seq (neuRNA-seq) labeling and capture were done by using Click-iT Nascent RNA Capture Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... click-labeling was performed for 30 min at room temperature using the Click-iT EdU-Alexa Fluor 647 Imaging Kit (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... the antibodies were labeled with Alexa Fluor® 568 and Alexa Fluor® 488 respectively using the Alexa Fluor® Antibody Labeling Kits according to the manufacturer’s manual (Thermo Fisher). The antibodies for fluorescence labeling were dissolved in PBS without any glycerol.
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bioRxiv - Plant Biology 2023Quote: ... DNA probes of NHX1 and SAL1 were synthesized and labeled using the Biotin 3′ End DNA Labeling Kit (Thermo Fisher Scientific, 89818). The EMSA was performed using the LightShift Chemiluminescent EMSA kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), human/mouse Foxp3 (clone 1054c ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were digested for 20 h at 37 °C and then labeled with the TMTsixplex Isobaric Labeling Kit (Thermo Fisher Scientific) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The purified anti-IFN-λ antibody was conjugated in-house with Alexa Fluor 647 by following the instructions of the antibody labeling kit (Invitrogen, A20186). The obtained labeled anti-IFN-λ antibody solution was used at 1d200 for staining ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were stained with an anti-human CD4 Ab that was pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), anti-human/mouse Foxp3 Ab pre-labeled with Alexa Fluor 555 (Molecular Probes ...
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bioRxiv - Cell Biology 2023Quote: ... Mono-biotinylated Fab’ fragments of goat anti-mouse Ig(G+M) were labeled with AF546 using an Alexa Fluor 546 antibody labeling kit (Thermo Fisher Scientific). The molar ratio of AF546 to Fab’ in the AF546-labeled Fab’ was ∼2 determined by a Nanodrop spectrophotometer (Nanodrop Technologies).
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bioRxiv - Microbiology 2023Quote: ... samples were labeled and pooled for multiplex relative mass spectrometry (MS) quantification with the TMTpro 16plex labeling kit (ThermoFisher Scientific, Waltham, MA) and underwent Liquid Chromatography with tandem mass spectrometry on an Orbitrap Elite mass spectrometer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... and Alexa Fluor 488 goat anti–rabbit secondary antibody were all supplied in the SelectFX Alexa Fluor 488 peroxisome labeling kit (Thermo Fisher Scientific). After washing with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μl of sample was saved for 3′ -biotin labeling for visualization using Pierce RNA 3′ End Biotinylation Kit (Life Technologies 20160) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein quantification of the glass bead protein extracts was conducted using the BCA Protein Assay kit (Thermo Fisher), and samples with equal amount of proteins were applied on SDS-PAGE gels ...
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bioRxiv - Microbiology 2021Quote: Purified ThsA protein was freshly prepared and protein concentration was quantified using Qubit Protein Assay Kit (ThermoFisher cat # Q33212). Protein was diluted in wash buffer to a concentration of 147 ng/μl ...
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bioRxiv - Neuroscience 2019Quote: Membrane proteins and cytosolic proteins were isolated using Mem-PER™ Plus membrane protein extraction kit (Thermo fisher scientific) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... and total protein concentration was quantified using a BCA protein assay (Pierce BCA Protein Assay kit, 23225, ThermoFisher Scientific). Samples were diluted to 0.8 ug/uL with a 1X cell lysis buffer provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the protein extract was then measured with a BCA Protein Assay Kit (Thermo Fisher Scientific). A volume of each protein extract corresponding to 14 mg protein was transferred into a new 5-mL tube containing 150 µL streptavidin beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Protein extracts from cells were assessed for protein concentrations using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Equal amounts of protein lysates were added to SDS-PAGE gels and electrophoresis was performed ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 500 ng ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 2 μg/μL with the IP lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: Protein concentrations were determined using the BCA protein assay (Micro BCATM protein assay kit; Thermo Fisher Scientific, MA, USA) and CLARIOstar microplate reader (BMG labtech Durham ...
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... Vibrant DiD membrane-labeling reagent was from Thermo Fisher (Waltham, MA). Brewer’s thioglycollate (TG) ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear labeling using Sytox Orange (SytoxO, 1:5000; S11368, Life Technologies) was applied to the brain tissues for 20 mins at room temperature (Faulkner et al. ...
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bioRxiv - Microbiology 2019Quote: ... prior to labeling with Alexa fluor 488 dextran (MW 10,000, Invitrogen) for 10 min in 10% RPMI ...
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bioRxiv - Bioengineering 2021Quote: ... Vybrant® DiD cell-labeling solution (Thermo Fisher; Waltham, MA, USA), via thin-film hydration method ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptide labeling with TMT 10plex (90110; Thermo Fisher Scientific, Waltham, MA) was performed per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody labeling was completed using anti-rabbit (Life Technologies A11034) or anti-mouse (Life Technologies A11029 ...
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bioRxiv - Cell Biology 2021Quote: ... Thoracic discs were dissected in chilled labeling media: Schneider’s medium (Gibco) containing 1% Pen/Strep (Caisson Labs) ...
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bioRxiv - Immunology 2022Quote: ... The following stains were used: Vybrant Dil cell-labeling solution (Invitrogen), Draq7 (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... Glucose labeling medium consisted of: MEM (1 g/L glucose; Gibco) supplemented with 1 g/L [U-13C6] glucose (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... after labeling with TMT reagents (TMT10plex label reagent set, Thermo Scientific), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Labeling of myelin debris with pHrodo Red SE (Thermo-Fisher Scientific) was carried out according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Pyrene-actin was prepared by labeling actin with pyrene iodoacetamide (Invitrogen) as described previously (44) ...
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bioRxiv - Biophysics 2020Quote: ... Labeling was performed by reacting Alexa Fluor 488-succinimidyl ester (Invitrogen) to 300 μg mCTxB in 100 mM sodium bicarbonate buffer pH 8.3 for 1 h under stirring at room temperature and purified using provided size exclusion chromatography resin ...
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bioRxiv - Molecular Biology 2022Quote: ... Labeling was terminated by the addition of 5% hydroxylamine (Acros Organics) for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Proximity labeling was performed by using Biotin-XX-Tyramide (ThermoFisher Scientific) dissolved in 50 mM Tris-HCl buffer (pH = 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: ... Fluorescence labeling was performed using DAPI (1:10,000, ThermoFisher Scientific, D1306), and Alexa Fluor 488- ...
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bioRxiv - Microbiology 2023Quote: ... and TMT10-plex TMT labeling (Thermo Fisher Scientific; catalog no. 90113) according to directions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... followed by labeling with CellTracker™ Green CMFDA dye (Invitrogen, C7025), for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... were switched to labeling media containing MEM for SILAC (Thermo Scientific) supplemented with dialyzed fetal bovine serum (Thermo Scientific) ...
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bioRxiv - Genomics 2024Quote: ... Quantitative PCR analyses were performed with SYBR green labeling (Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X) ...
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bioRxiv - Neuroscience 2024Quote: ... fluorescent dye (VybrantTM CM-DiI Cell-Labeling Solution; Invitrogen, CA, USA) was injected instead of virus solution ...
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bioRxiv - Neuroscience 2024Quote: ... Cell dye (Vybrant™ Dil Cell Labeling Solution, Thermo Fisher Scientific) was applied to the tips of the guide cannulae (to aid with histological identification of tip location) ...
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bioRxiv - Neuroscience 2024Quote: ... Microdrives were painted with Vybrant DiI labeling solution (Invitrogen, Waltham, MA) prior to implantation to verify recording position post hoc.
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bioRxiv - Cell Biology 2024Quote: ... This was followed by labeling with Lysotracker Deep Red (ThermoFisher Scientific) for 15 min ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were resuspended in PBS and semEV concentrations were estimated by BSA protein kit (Thermo Fisher, Pierce BCA Protein Assay Kit) and adjusted to 2mg/mL ...
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bioRxiv - Microbiology 2022Quote: Our metabolic click-labelling was based on the Click-iT HPG Alexa Fluor protein Synthesis Assay Kit (Click-iT HPG Alexa Fluor Protein Synthesis Assay Kit, ThermoFisher C10428) strategy ...
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bioRxiv - Physiology 2022Quote: ... 20 mg of pulverized tissue were dissolved in the assay buffer from the kit as suggested by the manufacturer and normalized to protein content by Pierce™ BCA Protein Assay Kit (ThermoFisher). 250 μg of protein was used for each reaction ...
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bioRxiv - Cancer Biology 2019Quote: Proteins were isolated using RIPA buffer and protein concentrations were determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Total RNA was extracted using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing proteins was collected and the protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Scientific). Immunoblotting analysis was carried out as previously described.(8 ...