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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Mouse Mesoderm Specific Transcript Protein MEST ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... Abundance of lncRNA:CR43651 and rpl32 transcript was estimated using SYBR green PCR mastermix (Applied Biosystems) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: Primary transcript pri-miR-K10/12 was analyzed after prior treatment with DNase I (Invitrogen) or TURBO™ DNase (Invitrogen) ...
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bioRxiv - Physiology 2020Quote: ... Transcript expression was measured using TaqMan Assays with AmpliTaq Gold DNA polymerase (Thermo Fisher Scientific) and target genes were compared to the mean of Rplp0 and 18S housekeeping genes using the ΔΔCT method ...
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bioRxiv - Immunology 2020Quote: ... Immunoglobulin epsilon (ε) heavy chain transcripts were amplified (StepOnePlus Real-Time PCR System, Applied Biosystems) using custom TaqMan gene expression kits (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: ... Transcript copies were detected using a fast SYBR Green PCR master mix (Applied Biosystems; 4385612) with the following primers ...
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bioRxiv - Immunology 2023Quote: ... Transcripts were quantified by real-time PCR analysis using the PowerUp SYBR Green (Applied Biosystems) and the QuantStudio 6 Flex (Applied Biosciences) ...
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bioRxiv - Developmental Biology 2023Quote: ... Target transcripts were analyzed using Applied 7500 sequence detector system (Applied Biosystems, Carlsbad, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Probe solutions consisting of cocktails of HCR probes for each transcript (Thermo Fisher Scientific, UK) were prepared with a final concentration of 24nM per HCR probe in HCR hybridization buffer ...
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bioRxiv - Physiology 2023Quote: ... Transcript quantity was calculated in Quant Studio SW (Thermo Fisher Scientific, Waltham, MA 02451, USA). Hprt1 transcript levels were used as a housekeeper reference.
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bioRxiv - Developmental Biology 2023Quote: ... and Zscan4 transcript abundance were determined using SYBR green gene expression assay (Thermo Fisher Scientific) in a QuantStudio 6 Flex system (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated fragmented nascent transcripts were enriched a total of three times using streptavidin Dynabeads (Invitrogen) and TRIzol LS reagent ...
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bioRxiv - Synthetic Biology 2024Quote: ... IL-21 cDNA transcript sequences were amplified by TaqMan probe based qPCR reactions (ThermoFisher, 4331182) using the Applied Biosystems TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Transcripts were quantified by real-time qPCR using Power SYBR Green PCR MasterMix (Applied Biosystems) in StepOne System (ABI) ...
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bioRxiv - Evolutionary Biology 2020Quote: The same amount of mutant and WT RNA was used to synthetize cDNA with High Capacity cDNA Reverse Transcript kit (Applied biosystems-Thermo Fisher Scientific, cat. 4368814). A total of four different samples (4 mutant and 4 WT ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Microbiology 2020Quote: ... nuclear soluble and chromatin-bound protein fractions were extracted from cells using the Subcellular Protein Fractionation Kit for Cultured Cells kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... all cDNA reactions were subjected to a specific PCR reaction for CHIKV genome amplification with the Phusion PCR kit (Phusion High-Fidelity DNA polymerase kit, Thermo Scientific, ref: F530L). 5 pairs of primers were used to span all CHIKV genome (Table 3 ...
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bioRxiv - Immunology 2020Quote: ... was used as soluble substrate to detect SARS-CoV-2 S-protein specific monoclonal antibodies and the final reaction was measured by using the Varioskan Lux Reader (Thermo Fisher Scientific) at a wavelength of 405 nm ...
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bioRxiv - Biochemistry 2021Quote: ... dissolved in water was added to the protein solution and the specific proteolytic digestion proceeded for 16 h at 37 °C in a thermomixer (Thermo Fisher Scientific). Digestion was centrifuged at 10000 rpm during 1 min and 4 μL of the resultant mixture of tryptic peptides was mixed with 0.3 μL of 90% formic acid and it was loaded into a metal coated borosilicate nanocapilar for mass spectrometric analysis.
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bioRxiv - Immunology 2020Quote: ... was used as soluble substrate to detect SARS-CoV-2 S-protein specific monoclonal antibodies and the final reaction was measured by using the Varioskan Lux Reader (Thermo Fisher Scientific) at a wavelength of 405 nm ...
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bioRxiv - Microbiology 2022Quote: ... membranes were washed with TBST for 30 min and specific protein bands were detected by chemiluminescence using SuperSignal West Femto maximum sensitivity substrate (Thermo Fisher Scientific). Band intensities were quantified relatively to the lane’s loading control using ImageJ (53).
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bioRxiv - Cell Biology 2023Quote: ... diluted with buffer C (the same recipe as buffer A but without NP-40) and precleared for non-specific binding by incubation with DynabeadsTM Protein G (Invitrogen, CA, USA) for one hour at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and non-specific IgG were incubated for 1 hour at room temperature with 20 µL aliquots of Protein A Dynabeads (Thermo Fisher, USA) mixed with 200 µL of PBST ...
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bioRxiv - Molecular Biology 2023Quote: The following primary antibodies were used for the immunological detection of specific proteins on PVDF membranes: anti-Tap (CAB1001 rabbit polyclonal antibody, Invitrogen, 1:10.000), anti-HA (12CA5 mouse monoclonal antibody ...
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bioRxiv - Biochemistry 2022Quote: ... Purified mouse SR was concentrated using a Pierce protein concentrator (Thermo Scientific) 10K molecular weight cut off filter ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μg/mL mouse protein laminin (Thermo Fisher Scientific 23017-015). 50 mm glass bottom dishes (WillCo GWSt-5040 ...
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bioRxiv - Neuroscience 2021Quote: ... monoclonal mouse anti-S6 ribosomal protein (MA515123, Thermo Fisher Scientific; 1:100), polyclonal rabbit anti-RPL24 (PA530157 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected using mouse monoclonal anti-Actin (Thermo Scientific, MS-1295), rabbit monoclonal anti-IFIT1 (Cell Signalling ...
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bioRxiv - Molecular Biology 2020Quote: ... V5-tagged proteins were detected with mouse monoclonal anti-V5 antibody (ThermoFisher) at 0.3 μg/ml ...
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bioRxiv - Genomics 2023Quote: ... 1 ug/mL Laminin mouse protein (Thermo Fisher Scientific; Cat. No. 23017015), 10 nM ROCK inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified with a Micro BCA protein assay kit (Thermo Scientific, Rockford, IL) using the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was adjusted using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). For co-immunoprecipitation assay ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentrations were determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), before extracts were stored at −80°C ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were determined using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were estimated using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific# 23227). 400-500μg of protein from the stably transfected HEK293 cells were used to immunoprecipitate FLAG-tagged MIB1 using EZview™ Red ANTI-FLAG® M2 affinity gel (MilliporeSigma# F2426 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a Pierce BCA Protein Assay kit (Thermofisher Scientific, 23225) colorimetric reaction and quantified on a SpectraMax i3 Multi-Mode Platform (Molecular Devices) ...
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bioRxiv - Immunology 2021Quote: ... Total protein concentration was assessed using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of whole-cell protein lysates samples were boiled in Laemmli loading buffer ...
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bioRxiv - Genetics 2021Quote: ... Protein amounts were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein (in vitro samples ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined via the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentration of lysates was measured using Pierce™ BCA Protein Assay Kit (ThermoFisher), to ensure equal loading of proteins ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were measured using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). Cytoplasmic and nuclear samples from each brain region containing 5μg total protein was boiled in Laemmli loading buffer and run on a 4%–20% Bio-Rad precast gel ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein content was quantified with a Pierce BCA Protein Assay Kit (Thermo Scientific). The lysate [50 µg protein/50 µL] was incubated with 0.5 µL of 1 M DTT solution at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and the protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo Fisher). 50 μg of protein was loaded into each lane of Mini-Protean TGX gels (Biorad ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein amount was confirmed with a BCA protein assay kit (Thermo Fisher Scientific). Proteins were reduced by treatment with 10 mM DTT for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific), then 30 μg protein lysates were mixed with NuPAGE™ LDS Sample Buffer (4x ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured with Pierce BCA Protein concentration assay kit (Thermo Fisher Scientific) on a spectrophotometer plate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2022Quote: ... Protein content was determined with the BCA Protein Assay kit (23225, Thermo Fisher Scientific). Equal amounts of protein were resolved on gels under reducing conditions ...
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bioRxiv - Genomics 2020Quote: ... Total protein content was quantified by Qubit™ Protein Assay Kit (Thermo Fisher Scientific). Sap containing 100 ug of protein was freeze-dried and resuspended in 5% SDS and 50mM triethylammonium bicarbonate (TEAB ...