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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 8 CYANO IMIDAZO 1 2 A PYRIDINE 2 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 0.2% SDS) at 50°C for 60 min on a Thermomixer (1200 rpm) followed by extraction with acid phenol (ThermoFisher, AM9712) and precipitation with 1:1 isopropanol:ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... using solvent A (0.1% formic acid in water) and separated on a C18 PepMap RSLC column (2 cm, 100 A; Thermo Fisher Scientific) using a linear gradient from 7 to 30% of solvent B (0.1% formic acid in acetonitrile ...
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bioRxiv - Cancer Biology 2020Quote: Lungs and LNs were excised and immersed in 4% paraformaldehyde with 10% sucrose and 7% picric acid in PBS for 2 hours and then embedded in Tissue-Tec OCT (Fisher Scientific). 10 micron sections were prepared and slides were stained for B220 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with 0.5 µM Lipi-Deep Red neutral lipid stain (Dojindo #LD04-10) for 2 hr and 5 µg/mL Hoeschst 33342 nucleic acid stain (Invitrogen #H3570) for 30 min at 37°C ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... each well was gently rinsed once with RPMI and then starved for 50 min in 2 mL of RPMI (without amino acids, glucose or FBS) with 20 μM Lysotracker Red DND-99 (Invitrogen, L7528). After 50 min of starvation ...
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bioRxiv - Microbiology 2020Quote: Dried samples were reconstituted in mobile phase A solvent (2% acetonitrile and 0.1% formic acid) for analysis on the Q-Exactive™ HF-X mass spectrometer (ThermoFisher Scientific), interfaced to the Easy nanoLC1200 HPLC system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Q-Exactive plus Mass Spectrometer (Thermo Fisher Scientific) at 300 nl/min via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... tryptic peptides obtained from StageTip-based SCX fractionation were reconstituted in 0.1% formic acid and loaded on a nanoViper 2 cm (3 µm C18 Aq) trap column (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (50 cm ...
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bioRxiv - Neuroscience 2022Quote: ... UM was changed for 2 days to an endothelial cell medium supplemented with retinoic acid (EC+RA: human Endothelial-SFM (Life Technologies), 1% platelet-poor plasma derived bovine serum (Alfa Aesar) ...
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bioRxiv - Microbiology 2022Quote: ... Amino acids were extracted on ice-cold lysis buffer [5:3:2 ratio of methanol-acetonitrile-water (Fisher Scientific, Pittsburgh, PA)] containing 3 µM of amino acid standards [Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... The dried peptide extracts obtained were dissolved in 12 μl of 0.05% trifluoroacetic acid in 2% acetonitrile and analyzed by online nanoLC using an Ultimate 3000 RSLCnano LC system (Thermo Scientific Dionex) coupled to an LTQ Orbitrap Velos mass spectrometer (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: A codon-optimized gene encoding for SARS-CoV-2 (331 to 528 amino acids, QIS60558.1) was expressed in Expi293 cells (Thermo Fisher Scientific) with human serum albumin secretion signal sequence and fusion tags (6xHistidine tag ...
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bioRxiv - Plant Biology 2019Quote: ... They were then diluted 10x with loading buffer (2% acetonitrile, 0.05% trifluoroacetic acid) and injected on a Fusion Tribrid orbitrap mass spectrometer (Thermo Fisher Scientific) interfaced to a Dionex RSLC 3000 nano-HPLC ...
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bioRxiv - Genomics 2020Quote: ... The resuspended peptides in sample loading buffer (2% acetonitrile and 0.05% trifluoroacetic acid) were separated and analysed on an UltiMate 3000 RSLCnano HPLC system (Thermo Fisher Scientific) coupled online to either a Q Exactive HF or an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... CAS number: 328–42–7), 2-Ketoglutaric acid, disodium salt, dehydrate (>99%, CAS number: 305–72–6) were purchased from Acros Organics, Belgium ...
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bioRxiv - Biochemistry 2022Quote: ... Then the pellets were resuspended in 10 mL of sterile water and transferred to a 15 mL falcon tube containing 2 g of acid washed glass beads (0.5 mm of diameter, Thermofisher, G8772-100G). Cells were then disrupted using a Fastprep system (MP Biomedicals) ...
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bioRxiv - Biophysics 2022Quote: ... Electron microscopy grids were prepared in 2% (w/v) phosphotungstic acid (pH 7.5) and were examined in a Talos L120C transmission electron microscope (FEI-Thermo Fisher Scientific). Nanodisc samples were frozen in aliquots and stored at −80°C.
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bioRxiv - Microbiology 2022Quote: ... and cells were blocked in 500 μl of FACS buffer (2% FBS and 0.2 mM ethylenediaminetetraacetic acid (EDTA) in PBS (Gibco, Thermo Fisher Scientific)) ...
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bioRxiv - Molecular Biology 2023Quote: Phosphor imaging of [γ-32P] radio-labelled DNA sequences was accomplished through 6% denaturing PAGE constituted of 1X TBE (89 mM Tris base, 2 mM EDTA and 89 mM boric acid, Fisher Scientific) (57) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dried peptide extracts obtained were dissolved in 17 μl of 0.05% trifluoroacetic acid in 2% acetonitrile and analyzed by online nanoLC using an Ultimate 3000 RSLCnano LC system (Thermo Scientific Dionex) coupled to an LTQ Orbitrap Velos mass spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Q-Exactive plus Mass Spectrometer (Thermo Fisher Scientific) or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface at 300 nl/minute via the analytical column using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... the cornea containing molten scaffold medium was placed epithelial side down in a 6 well-plate containing 400 µl of Ethylene glycol-O,O’-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA) (Fisher scientific) and was kept in a CO2 (5% v/v ...
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bioRxiv - Microbiology 2023Quote: C18 purified digests were re-suspended in Buffer A* (2% acetonitrile, 0.01% trifluoroacetic acid) and separated on a Ultimate 3000 UPLC (Thermo Fisher Scientific) with a two-column chromatography set up composed of a PepMap100 C18 20 mm x 75 μm trap and a PepMap C18 500 mm x 75 μm analytical column (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were concentrated onto the trap column at 5 μl/min for 5 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap Eclipse Mass Spectrometer (Thermo Fisher Scientific) or a Orbitrap Fusion Lumos equipped with a FAIMS Pro interface both at 300 nl/minute via the analytical columns using a Dionex Ultimate 3000 UPLC (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: Proteome samples were re-suspended in Buffer A* (0.1% trifluoracetic acid, 2% acetonitrile) and separated using an Ultimate 3000 UPLC (Thermo Fisher Scientific) equipped with a two-column chromatography set up composed of a PepMap100 C18 20 mm x 75 μm trap and PepMap C18 500 mm x 75 μm analytical column (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... and cells were blocked in 500 μl of FACS buffer (2% FBS and 0.2 mM ethylenediaminetetraacetic acid (EDTA) in PBS (Gibco, Thermo Fisher Scientific)) ...
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bioRxiv - Pathology 2024Quote: ... the peptide was reconstituted in 2% acetonitrile/0.1% formic acid (FA) and analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific) coupled online to an UltiMate 3000 RSLCnano System (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: Dried peptides were suspended in 0.1% formic acid (FA) with 2% ACN and analyzed by LC-MS/MS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) connected to an EASY nLC 1200 (buffer A ...
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bioRxiv - Cell Biology 2024Quote: ... iRBCs were washed twice with media to remove extracellular merozoites before being aliquoted in triplicate into a 96-well U-bottomed plate and stained with the nucleic acid dye SYTO-61 (2 μM) (Thermo Fisher) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Pathology 2019Quote: PMLECs between the 3rd and 5th passages were seeded (8 × 104/well) in 2-well Permanox chamber slides from Lab-tek (Thermo Scientific, Nunc). The cells were incubated at 37°C and 5% CO2 for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... U-2 OS cells were cultured to 80% confluency in Nunc™ 8-well Lab-Tek II Chambered Coverglass slides (Thermo Scientific). Upon infection ...
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bioRxiv - Cell Biology 2021Quote: ... BV-2 immortalized microglial cells (Blasi et al. 1990) (passage 3 to 8) cultured in macrophage serum-free medium (ThermoFisher 12065-074) were used ...
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bioRxiv - Biochemistry 2022Quote: ... At culture day 8 cytosolic Ca2+ levels in primary neurons were determined using the Ca2+-sensitive fluorescent indicator Fura-2-AM (Molecular Probes/Invitrogen). Cells were loaded with Fura-2 in sterile-filtered HEPES-buffered saline (HBS ...
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bioRxiv - Cell Biology 2021Quote: Proteins were separated by SDS-PAGE (8 % gels) and then transferred to a nitrocellulose membrane with an iBLOT 2 Gel Transfer Device (ThermoFisher Scientific, IB21001). Nitrocellulose membranes were blocked for 1 h with 5 % dried milk resuspended in TBS-Tween 1 % and incubated over-night with primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: AXL −ve and AXL-positive cells from H1650 and PC9 were FACS-sorted and cultured for 2 days in an 8-well chamber slide system (LAB-TEK, Thermo Fisher Scientific). H1650 ...
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bioRxiv - Immunology 2021Quote: ... Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8, 150 mM NaCl) and 5x Sypro Orange (Invitrogen, stock 5000x) in a total volume of 20 μl ...
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bioRxiv - Immunology 2020Quote: ... 100 U/ml penicillin,100 μg/ml streptomycin, 8% (vol/vol) fetal bovine serum (all Bio&Sell, Nürnberg, Germany) and 2% (vol/vol) chicken serum (ThermoFisher Scientific, Waltham, USA), with the addition of recombinant soluble chicken BAFF and chicken CD40L ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated hippocampal cells were plated at 2 × 104 cells per well on Lab-Tek II 8-well chamber coverglasses (Thermo Fisher Scientific) coated with polyethylenimine and BioCoat poly-D-lysine (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... Lung organoids were maintained for experiments between passage 2-8 prior to dissociation with TrypLE and seeding onto Geltrex (Gibco, Cat. A1413201) -coated plates supplemented with Y-27632 (Selleck Chemicals ...
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bioRxiv - Microbiology 2023Quote: ... Western blotting was performed on 8% SDS-PAGE gels and transferred to polyvinyldifluoride (PVDF) membranes using the iBlot 2 (Thermo Fisher Scientific). Membranes were blocked and probed with purified polyclonal goat antibodies to LiaX diluted 1:3000 and horseradish peroxidase-conjugated mouse anti-goat antibody diluted 1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by 8% acrylamide SDS-PAGE gels and then transferred to a nitrocellulose membrane with an iBLOT 2 Gel Transfer Device (ThermoFisher Scientific, IB21001). Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... iN cells (1 × 104 per well) were plated together with mouse glial cells (2 × 104 per well) onto 8-well chamber glass slides (ThermoFisher, catalog #: 154941) in the Growth Medium ...
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bioRxiv - Immunology 2023Quote: ... pH 6.8 buffer containing 2% SDS and 20% glycerol) with the addition of protease and phosphatase inhibitors (A32953, A32957 respectively, Thermo Fisher Scientific). Proteins were separated using SDS-PAGE under reducing conditions ...
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bioRxiv - Microbiology 2023Quote: ... Grids were blotted for 2 s in 100 % humidity at 8-12 ℃ and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected in counted mode in EER format on a CFEG-equipped Titan Krios G4 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 6-8-week-old WT mice were randomly selected to receive 2% dextran sodium sulfate (DSS) 40-50,000 MW (Affymetrix, Santa Clara, CA) in drinking water for 7 days followed by normal drinking water afterwards ...
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bioRxiv - Cell Biology 2020Quote: ... or Alexa647 NHS ester (ThermoFisher, A20006) for 15 minutes at RT ...
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bioRxiv - Immunology 2021Quote: ... acetyl ester (CM-H2DCFDA) probe (Invitrogen) for 45 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... carboxyfluorescein succinimidyl ester (Thermo Fisher Scientific) at 37°C for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: BODIPY TMR-X NHS Ester (ThermoFisher) was used to fluorescently label LRRK2RCKW and LRRK1RCKW ...