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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 7 Propyl 3 trifluoromethyl 1 2 benzisoxazol 6 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Lungs and LNs were excised and immersed in 4% paraformaldehyde with 10% sucrose and 7% picric acid in PBS for 2 hours and then embedded in Tissue-Tec OCT (Fisher Scientific). 10 micron sections were prepared and slides were stained for B220 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The redox homeostasis of promastigotes was monitored both qualitatively and quantitively by 2-7 dichlorodihydro fluorescein diacetate (DCFDA) (Life Technologies, USA) staining ...
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bioRxiv - Neuroscience 2021Quote: ... Percent of cell death was determined by staining with 7 μM Hoechst 33342 and 2 μM propidium iodide (PI) (Invitrogen, USA). PI were diluted in warm neuronal culture medium and incubated for 5 min and then images were taken by a Zeiss microscope equipped with automated computer assisted software (Axiovision 4.6 ...
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bioRxiv - Cell Biology 2021Quote: ... day 7 and day 19 samples all cells were adherent and collected using 2 ml/well accutase (Thermo Fisher Scientific, A1110501) and a cell scraper ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1x PBS twice and incubated for 20 min in dark at 37°C with 20 µM of H2DCFDA (2’,7’- dichlorodihydrofluorescein diacetate; I36007, Thermo Fisher) diluted in serum-free medium ...
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bioRxiv - Immunology 2022Quote: ... reactions were heated to 70 °C for 2 min and 15 μl loaded on 15% polyacrylamide gels containing 7 M urea (Invitrogen, EC8852BOX). Low range ssRNA Ladder (NEB ...
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bioRxiv - Pathology 2023Quote: ... The mRNAs were quantified using individual TaqMan assays described in Supplemental Table 2 on an ABI QuantStudio 7 Real-Time PCR machine (Applied Biosystems) using TaqMan Fast Advanced Master Mix (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: Gene expression of targets listed in supplemental table 2 were analysed by SYBR green-based qPCR analysis using Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Thermo Scientific), following the standard PCR cycling sequence using GAPDH as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... plated at a density of 7 x 106 cells in a 175 cm2 tissue culture flask in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Library concentration was normalized to 2 nM and concentrations validated by qPCR on a ViiA-7 real-time thermocycler (Applied Biosystems), using qPCR primers recommended in Illumina’s qPCR protocol and Illumina’s PhiX control library used as a standard ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated with fresh DMEM containing DDAO galactoside (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-Galactopyranoside) (Molecular Probes™) for 2 hours ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...
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bioRxiv - Plant Biology 2024Quote: ... Ten-μg protein of each sample were separated by electrophoresis in 15% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Roth) for 7 min at 20V using the iBlot 2 Dry Blotting System (ThermoFisher Scientific), before blocking in TBS-T with 5% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... 7 μl per injection was loaded onto a 2 mm by 0.3 mm Acclaim PepMap C18 trap column (Thermo Fisher Scientific) in 0.1% TFA at 15 μl/min before the trap being switched to elute at 0.25 μl/min through a 50 cm by 75 μm EASY-Spray C18 column ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2020Quote: ... or a 1:1 mixture of two siRNA to CHMP2A (5’-CAGGCCGAGAUCAUGGACAUG-3’ and 5’-GAAGAUGAAGAGGAGAGUGAC-3’) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2019Quote: ... and 2 unaffected individuals (II.1 and II.3) were analyzed with the Affymetrix GeneChip® Mapping 250K Array Xba 240 (Affymetrix, Santa Clara, CA). Sample processing and labelling were performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were washed 3 times in DPBS and incubated for 2 hours with Alexa Fluor 647-conjugated anti-mouse secondary antibody (Thermo Fisher Scientific, 1:1000) in blocking buffer at RT ...
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bioRxiv - Systems Biology 2023Quote: ... 1 vein graft slide containing 2-4 cross sections was double stained with the general macrophage marker Mac-3 (rat anti-mouse, Fisher Scientific, B550292, 1:600) and either iNOS (rabbit anti-mouse ...
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bioRxiv - Microbiology 2023Quote: ... determined by preparing a slurry with 1 part soil to 2 parts deionized water and measuring (n=3) with an Orion Star A111 pH Meter (Thermo Scientific, Waltham, MA, USA), was 5.87 ± 0.42 in November 2020 and 6.28 ± 0.07 in April 2021.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... was performed with Hieff™ qPCR SYBR® Green Master Mix (Yeasen, China) in a QuantStudio 6&7 Flex Real Time PCR System (Applied Biosystems, Life Technologies, Milan, Italy). The relative expression levels of IDH among the three body regions were calculated using the 2−ΔΔCt method [71] ...
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bioRxiv - Microbiology 2020Quote: ... epidermidis (Thermo scientific; 3 μg/ ml in 1% BSA). MSC’s were labelled with anti-vimentin monoclonal antibody (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... 1.1ul (4U) FastDigest-HindIII (prediluted 1/3) (Thermo Fisher) and 11.1ul 20X primer-probe mix ...
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bioRxiv - Biophysics 2021Quote: ... Mirius (1:3) in Opti-MEM media (Thermo scientific). Cell confluency was allowed to reach 70-80% prior to transfection ...
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bioRxiv - Bioengineering 2021Quote: ... or rabbit anti-caspase-3 (1:500, ThermoFisher #700182) followed by Alexa-conjugated anti-mouse or anti-rabbit secondary antibodies for 8 hours each at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... or (3) 1:20 AlexaFluor 647 phalloidin (ThermoFisher #A22287) (Figure S2F ...
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bioRxiv - Cell Biology 2021Quote: ... in a 1:3 ratio using lipofectamine (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... in a 1:3 ratio using lipofectamine (Life Technologies) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... PBS containing 1 µM TO-PRO 3 (Thermo Scientific) was applied to each section followed by 3 washes with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... with TO-PRO-3 (#T3605, ThermoFisher, 1:1000 dilution).
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bioRxiv - Neuroscience 2021Quote: ... 1-hexanol (99%, ACROS Organics, CAS 111-27-3), 2-oxovaleric acid (>98% ...
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bioRxiv - Microbiology 2021Quote: ... 3-cholamidopropyl dimethylammonio 1-propanesulfonate (CHAPS; zwitterionic; Thermo Scientific). In addition ...
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bioRxiv - Developmental Biology 2022Quote: IF blocking buffer: 1/3 Blocker Casein (ThermoFisher 37528), 2/3 HEPM with 0.05% Triton X-100
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bioRxiv - Molecular Biology 2021Quote: ... containing 1 M sodium chloride (S671-3, Fisher Scientific). The labelling protocol was carried out at pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-Neuroligin-3 (1:1000, Thermo Fisher Scientific), rabbit anti-COX-2 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and TO-PRO-3 (1:10,000, Thermo Fisher Scientific). Images were taken using a 20x oil immersion and a 10x dry objective on a Leica TCS SP8 confocal microscope.
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bioRxiv - Developmental Biology 2023Quote: ... Immunofluorescence blocking solution: 1/3 Blocker Casein (ThermoFisher Scientific), 2/3 HEPM with 0.05% TX-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... and benzonase (1:100, Fisher Scientific, 70-664-3) on ice for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... 1 μg/ml -CD28 and 1:50 diluted conditioned supernatant from COS-7 cells transfected with a pcDNA1 vector (Invitrogen) encoding for recombinant chicken IL-2 (a kind gift from prof ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Microbiology 2019Quote: ... DAPI (1:1000, 2% BSA:PBS) (Invitrogen) and phalloidin-Alexa647 (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1-2 μl of GlycoBlue (Invitrogen) was used as co-precipitant ...
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bioRxiv - Bioengineering 2020Quote: ... 1:2 Neurobasal (Thermo Fisher 21103049), 0.5X B17 (Invitrogen 17504044) ...
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bioRxiv - Microbiology 2021Quote: ... Enhancers 1 and 2 (Thermofisher Scientific) were added to the culture 18 h post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... induced with 1% N-2 (Gibco) and 0.1% B-27 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM GlutaMAX-1 (ThermoFisher #35050061), 0.1 mM MEM nonessential amino acids and 100 U/mL penicillin and 100 µg/mL streptomycin ...
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bioRxiv - Neuroscience 2020Quote: ... Cox-2 (1:500, Thermo Fisher); and β-actin (1:10,000 ...