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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 6 METHYL 7 1 METHYL 2 OXO PROPOXY 2 3 DIHYDRO 1H CYCLOPENTA C CHROMEN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... and 2-3 μL of the freshly phase-separated sample was placed into a chamber made on a glass slide (Fisher Scientific 3” × 1” × 1 mm). The chamber made by using double-sided tape was then sealed with a square coverslip to avoid evaporation of the sample ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Cell Biology 2019Quote: ... The GST-fused protein was eluted with 1 ml GST elution buffer and the eluted sample was dialyzed overnight at 4°C in 2 l 1x PBS in a Slide-A-Lyzer Dialysis Cassette G2 10 000 MWCO (Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Jurkats were treated with 150mJ of UV energy and incubated for 2-4 hours at 37°C in complete media consisting of RPMI 1640 Media (Gibco, 11875101), 10% FBS (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of the polar phase was collected and vacuum-dried at 4°C and subsequently derivatized with 2% (w/v) methoxyamine hydrochloride (Thermo Scientific) in pyridine for 60 min following by 30 min sialyation N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed for 15 min at 37°C in 4% paraformaldehyde and incubated with 2 μM Bodipy 493/503 (Bodipy 493, Molecular Probes) and 1 μg/ml of DAPI as published (9) ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining 50 μL were incubated at 4 °C for 2 h with 40 μg of HisPur Ni–NTA magnetic beads (Thermo Scientific) pre-equilibrated in binding buffer ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
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bioRxiv - Physiology 2022Quote: ... The corpus was incubated in 50% betaisodona/PBS (Mundipharma GmbH) for 30 min at 4°C before being washed in different solutions for 2 min each: PBS (ThermoFisher Scientific), 0.1% octenidin in ddH2O (Serva Electrophoresis) ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20) replacing the buffer every 2 hours and incubated overnight at 4°C with the appropriate secondary antibodies (ThermoFisher Scientific), anti-Rabbit-Alexa 488 (RA3) ...
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Systems Biology 2020Quote: Similar to our recent report,16 1 mg nuclear protein extracted from Raw 264.7 macrophage cells was incubated overnight at 4°C with 2 nmole UNC0965 pre-coupled to 50 μl neutravidin-agarose (Thermo Fisher), and washed three times with 1 ml lysis buffer to remove non-specific bound proteins ...
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bioRxiv - Genetics 2021Quote: ... The lysate was incubated for 2 hours in a rotating wheel at 4°C with Dynabeads Protein A (#10002D Dynabeads Protein A, ThermoFisher Scientific) as a preclearing step ...
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bioRxiv - Developmental Biology 2020Quote: ... while the remainder was immunoprecipitated for 2 hr at 4°C in the following antibodies: rabbit anti-GFP (Invitrogen, A-11122), rabbit anti-Cen ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Thermo Scientific, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... The lysate was cleared by centrifugation and incubated for 2 hours at 4°C with Tag specific magnetic beads (Dynabeads™ M-280 Streptavidin/Invitrogen for immunoprecipitation of Avitag-ZFP462 and Avitag-GLP ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were dissociated into single cells with TrypLETM for 4-5 minutes at 37 C and seeded into 2 % Geltrex coated 96- or 12-well plates (Fisher Scientific) in Essential 8TM Medium (Gibco ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated at 4°C covered from light for 30 minutes with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher) while in PBS and then resuspended in rat anti-mouse CD16/32 (Fc block ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated at 4°C covered from light for 30 minutes with 2 ul/ml Fixable Viability Dye eFluor506 (Thermo Fisher) while in FACS buffer (PBS with 2% newborn calf serum ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VIPR particles were centrifuged at 14,500 xg for 3 minutes at 4°C and washed three times in PBS while the beads were immobilized in an Invitrogen DynaMag-2 magnet (Thermo Scientific). VIPR particles were resuspended in sterile PBS at a concentration of 5×108 particles per ml and stored at 4°C.
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 50 μL were incubated at 4°C for 2 h with 40 μg of pre-equilibrated HisPur Ni–NTA magnetic beads (Thermo Scientific) in a final volume of 200 μL ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded for 2 h at 4°C on a bi-directional rotator with 2 mM Ca2+ medium containing drugs 30 μM pancuronium (Fisher Scientific), or 38.9 μM curare (Sigma-Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... Cells were loaded at 37°C for 60 minutes with 4 μM Fura-2-AM in the presence of 1X Power Load permeabilizing reagent (Thermo Fisher). Injection of AngII (10-8M) ...
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bioRxiv - Molecular Biology 2023Quote: ... Inoculated cells were incubated for 1 h at 37°C and then overlaid with a 1:1 mixture of 1.2% Oxoid agar and 2× DMEM (Gibco) with 10% (vol/vol ...
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bioRxiv - Biophysics 2020Quote: All the in vitro transcription experiments were performed in NEB’s transcription buffer (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine) with 1 mM NTPs (R1481, Thermo Scientific), 22 wt% glycerol ...
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bioRxiv - Cancer Biology 2024Quote: ... an additional 150 µL of a 1:1 mixture of NK-Xpander complete medium:OncoPro complete medium containing 500 U/mL rhIL-2 and 10 µM Invitrogen™ CellEvent™ Caspase-3/7 Red Detection Reagent (Thermo Fisher Scientific, cat# C10430) was added to each well ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or 10 mM 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA, Thermo Fisher Scientific, Waltham, MA, USA). The cells stained with TMRE or NAO were incubated for 15 minutes at 37◦C whereas cells stained with HE and DCFH-DA were incubated for 30 minutes at 37◦C in the dark ...
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bioRxiv - Immunology 2022Quote: ... The cells were incubated in presence of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, 25 mM) (Invitrogen) for 30min at 37 °C and 5% of CO2 ...
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bioRxiv - Microbiology 2021Quote: ... was assessed by the cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (CFDA) (10 µg/ml, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with 2 μM propidium iodide (PI) and 7 μM Hoechst 33342 (ThermoFisher) for 5-10 min and imaged under a Zeiss Observer Z1 microscope ...
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bioRxiv - Physiology 2023Quote: ... followed by 7 days of decalcification with Shandon’s TBD-2 (Thermo Scientific, Waltham, MA, USA) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... SuperSignal Chemiluminescent substrate and 2’,7’-dichlorodihydrofluorescein diacetate acetyl ester (H2DCFDA) were from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cellular reactive oxygen species (ROS) levels were detected using 2’,7’ dichlorodihydrofluorescein diacetate (H2DCFDA; Invitrogen). Cells were treated in the absence or presence of drugs for 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Neuroscience 2021Quote: ... The nuclei of astrocytes and autaptic neurons were visualized by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI)-containing mounting medium (ProLong® Gold Antifade Reagent with DAPI, Thermo Fisher Scientific). Only neurons with one nucleus were analyzed when counting synapse numbers.
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bioRxiv - Microbiology 2022Quote: ... 0.1% Triton X-100 in PBS for 20 minutes, then were actin stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) nucleic acid stain (Thermo Fisher Scientific, USA) in PBS for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The cells were again washed and incubated for 10 min in a solution of 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific). Cells were imaged using a Leica DM IRB epifluorescence microscope ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... sections were rinsed three times for 10 min each and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Immunology 2020Quote: ... A viability dye was included in all Duraclone panels (Live/dead fixable dead cell stain kit from ThermoFisher Scientific or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate from ThermoFisher Scientific (Courtabœuf, France)) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa-Fluor 594 labelled goat anti-rabbit IgG, ProLong Gold antifade reagent with DAPI (4’, 6-diamidino-2-phenylindole) (Life technologies-Invitrogen, USA), ATP Affinity kit (no ...
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bioRxiv - Molecular Biology 2022Quote: ... All sections were mounted with 50μl of ProLong Gold Antifade mounting media containing a fluorescent nucleic acid dye 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, P36931) before imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were rinsed three times with 1X PBS and coated with ProLong Gold antifade mounting medium with DAPI (4’,6-diamidino-2-phenylindole, 100 ng/ml) (Life Technologies, P-36931) on slides ...
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bioRxiv - Bioengineering 2023Quote: ... These slices were then fixed in 4’,6-diamidino-2-phenylindole (DAPI) mounting media (Fluoromount-G® with DAPI, Thermo Fisher Scientific, USA), and washed twice with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then stained with 4′,6-diamidino-2-phenylindole (DAPI) to stain cell nuclei and mounted with Immu-Mount mountant (Thermo Scientific, Cat# 9990402) onto microscope slides.
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bioRxiv - Microbiology 2022Quote: ... and blocked for 45 min in 2% bovine serum albumin (BSA) in PBS prior to staining with DAPI (4=,6-diamidino-2-phenylindole) and mounted in Prolong Diamond antifade (Molecular Probes, Life Technologies). Images were captured using a Nikon Eclipse Ti confocal microscope (Nikon Instruments ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
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bioRxiv - Neuroscience 2021Quote: ... and 1 mM of the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (ThermoFisher Scientific). The samples were further ruptured using a syringe and centrifuged at 14,000g for 30 min at 4°C ...